scholarly journals Determination of biologically active substances and antioxidant activity of the aerial parts of Baikal aconite Aconitum baicalense Turcz

Author(s):  
Svetlana S. Kravtsova ◽  
Maria P. Sandu ◽  
Larisa A. Baygulova ◽  
Victor V. Khasanov
ÈKOBIOTEH ◽  
2021 ◽  
Vol 4 (1) ◽  
pp. 33-39
Author(s):  
V.P. Kurchenko ◽  
◽  
N.V. Sushinskaya ◽  
K.I. Maiorava ◽  
E.I. Tarun ◽  
...  

The study of the composition of biologically active substances, alcoholic extracts from flowers of Aeculus hippocastanum L. According to the results of HPLC-MS and GC-MS analyzes, the extract contains the main amounts of phenolic compounds: quercetin, epicatechin, kaempferol. In addition, the extract contains fatty acids and their esters, alcohols, 3-deoxy-d-manno lactone, 1,2,3,5-cyclohexantethrol, α-methyl-mannofuranoside, γ sitosterol. Antioxidant activity of an extract from flowers of A. hippocastanum is associated with the peculiarities of the compositionof biologically active substances. The toxological-hygienic assessment of flowers of this species in acute and subacute experiments showed that it belongs to the 4th hazard class (low hazard).


2017 ◽  
pp. 145-151
Author(s):  
Ефим (Efim) Авраамович (Avraamovich) Краснов (Krasnov) ◽  
Елена (Elena) Евгеньевна (Evgen'evna) Савельева (Savelyeva) ◽  
Надежда (Nadezhda) Кирилловна (Kirillovna) Рыжакова (Ryzhakova) ◽  
Ярослав (Yaroslav) Евгеньевич (Evgen'evich) Решетов (Reshetov) ◽  
Альбина (Al'bina) Равильевна (Ravil'evna) Гатауллина (Gataullina)

To determine the content of the dominant groups of biologically active substances (tannins, polysaccharides, flavonoids) and bioelements in aerial parts of ten species of the Rosaceae family, which grows in Siberia: Potentilla anserina L., P. longifolia Willd., P. canescens Besser, P. bifurca L., P. tergemina Sojak, P. argentea L., P. goldbachii Rupr., P. sericea Dulac., Agrimonia pilosa Ledeb., Filipendula ulmaria (L.) Maxim. To determine the concentrations of essential and conditionally essential trace elements defined by the method of atomic emission spectroscopy. First identified plants of the genus Potentilla (P. canescens, P. tergemina) and Filipendula (F. ulmaria), accumulate significant quantities of lithium – 14,6, and 11,0 13,3 mg/kg, respectively, making them promising for further studies on the isolation and identification of active substances with the aim of creating innovative drugs. Investigated the content of heavy metals (Pb, Hg, As, Cd), the values of which meets regulatory requirements. Useful properties of the examined species family Rosaceae are determined by the content of biologically active substances (flavonoids, polysaccharides, tannins) and the complex of macro - and micronutrients. It is revealed that a dominant position in the spectrum belongs to the macronutrients potassium, calcium and magnesium (4,300 to 27,000 mg/kg). Among micronutrients the leading role is copper, followed by iron, silicon and manganese having important biological value, in particular, involved in the process of hematopoiesis.


Author(s):  
H. Yu. Teslyar ◽  
M. Ya. Smolinska ◽  
I. Ya. Kotsyumbas ◽  
N. M. Chyhyn ◽  
N. G. Rohulia ◽  
...  

An analytical method for the quantitative determination of antioxidant sodium metabisulphite in veterinary drugs has been proposed by spectrophotometric method. Based on the literature data, the optimal conditions of the analytical reaction were selected experimentally. The dependence of the value of the analytical signal on the temperature of the reaction medium, concentration of p-rosaniline and duration of the reaction was investigated to establish the optimal conditions of the analytical reaction and obtain a stable analytical signal. The stability of the colored analytical form in time was also checked and the linear dependence of the value of the analytical signal on the concentration of sodium metabisulphite was investigated. The analytical reaction at room temperature is optimal. The maximum analaytical signal is achieved by carrying out the analytical reaction for 10 minutes and then practically does not change for an hour. To achieve the maximum analytical signal, it is necessary to use a 20-fold excess of dye relative to sodium metabisulphite. The analytical signal remains stable only for the first hour, then gradually begins to decrease. Metrological characteristics of the method of determination of metabisulphite in veterinary drugs are calculated, the limits of spectrophotometric determination are 0.33 – 2.50 μg/ml. The correctness of the developed method was checked on model solutions by the method of "introduced-found" method of comparison in the presence of various biologically active substances that are part of drugs together with sodium metabisulphite. The content of sodium metabisulphite in veterinary drugs of domestic and foreign production at different intervals of their storage time was established. It is shown that the content of sodium metabisulphite in drugs decreases during their storage time, until complete disappearance, which directly affects the content of the active substance, because in the absence of antioxidant oxidative processes with biologically active substances begin to take place.


2021 ◽  
Vol 4 (1) ◽  
pp. 126-130
Author(s):  
V. R. Hamada ◽  

This article shows the possibility of using a biotechnological method to obtain biologically active compounds based on in vitro cultivation of callus cultures. The callus biomass of Adonis vernalis was obtained in vitro by using the biotechnological method. The extracts based on callus biomass of Adonis vernalis were obtained. The content of flavonoids and phenolic compounds was determined. The antioxidant activity of callus biomass of Adonis vernalis has also been studied. The research results show that the content of biologically active substances and biological activity in callus biomass does not differ from plant raw materials.


2019 ◽  
Vol 5 (10) ◽  
pp. 32-39 ◽  
Author(s):  
A. Sarukhanov ◽  
A. Morozova ◽  
N. Vasilyeva ◽  
D. Krylenkin

General information about biologically active substances flavonoids is concisely presented. The need for the quantitative determination of its content in products and the influence of product processing on flavonoid’s content is justified. Methods of qualitative and quantitative determination of flavonoids content in various products, medications and raw materials were studied. Based on this study the method of quantitative determination of flavonoid’s in samples of irradiated coriander is compiled and tested. Further ways to improve and adjust the compiled method for flavonoid’s content determination in irradiated samples via spectrophotometric analysis is considered.


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