scholarly journals Bacterial aggregation in infected root canal

2005 ◽  
Vol 5 (4) ◽  
pp. 35-39 ◽  
Author(s):  
Amela Lačević ◽  
Nurija Bilalović ◽  
Aida Kapić

The aim of this study was to investigate different microbial morphotypes in the root canal infection associated with chronic diffuse periapical lesion. In forty cases of asymptomatic teeth with radiographically diagnosed diffuse periapical lesion we took specimens of infected tissue from the root canals at the beginning of endodontic treatment. Fixation and four different staining methods of the specimens were obtained to provide microscope examination. All examined root canal specimens were heavily infected by bacteria. The most commonly identified were cocci 92 %, small mostly G+ diplococci and large G+cocci in clusters and grapelike groups, bacilli found in 67%, coccobacilli 37%, fungi 17%, and spirochetes in 5%.

2020 ◽  
Vol 75 (10) ◽  
pp. 553-563
Author(s):  
Maheshan Pillay ◽  
Martin Vorster ◽  
Peet J Van der Vyver

Instrument fracture is a serious complication during endodontic treatment of teeth, having an adverse effect on the outcome of the nickel titanium (NiTi) treatment, especially if the fracture prevents apical access to the infected root canal. Despite the advent of NiTi files, the risk of fracture during the endodontic preparation of root canals, especially in severely curved canals, remains a serious concern. The fracture of NiTi files during preparation may result in a compromised prognosis for the tooth. In the presence of periapical lesions, instrument fracture may reduce the chances of successful healing.


2010 ◽  
Vol 9 (1) ◽  
pp. 63
Author(s):  
Indrya Kirana Mattulada

Bacteria and their products play a fundamental role in the initiation, propagation andpersistence of pulpal and periradicular diseases. Consequently one of the major goalsof endodontic treament is to eliminate all bacteria from the root canal space.Elimination of microorganism from infected root canal is a complicated task.Numerous measures have been described to reduce the numbers of root canalmicroorganism, including the use of various instrumentation techniques, irrigationregimens, and intracanal medication. In endodontic treatment, there are severalmedicament used traditionally. They are phenolic, aldehyde, halogen, calciumhydroxide, and antibiotic. The aim of this paper is to discuss about indication,contraindication and effect of each medicament.


2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Sheela B. Abraham ◽  
Farah al Marzooq ◽  
Wan Harun Himratul-Aznita ◽  
Hany Mohamed Aly Ahmed ◽  
Lakshman Perera Samaranayake

Abstract Background There is limited data on the prevalence of Candida species in infected root canal systems of human teeth. We attempted to investigate the prevalence, genotype, virulence and the antifungal susceptibility of Candida albicans isolated from infected root canals of patients with primary and post-treatment infections in a UAE population. Methods Microbiological samples from 71 subjects with infected root canals were aseptically collected, and cultured on Sabouraud dextrose agar, and C. albicans was identified using multiplex polymerase chain reaction, and the isolates were further subtyped using ABC genotyping system. Their relative virulence was compared using further four archival samples of endodontic origin from another geographical region, and four more salivary isolates, as controls. The virulence attributes compared were biofilm formation, and production of phospholipase and haemolysin, and the susceptibility to nystatin, amphotericin B, ketoconazole, and fluoconazole was also tested. Results 4 out of 71 samples (5.6%) yielded Candida species. On analysis of variance among the groups, the intracanal isolates, mainly Genotype A, possessed a high degree of phospholipase and haemolysin activity (p < 0.05). The UAE and Finland isolates were stronger biofilm formers and had superior phospholipase production capacity compared with the salivary isolates. All isolates were sensitive to the antifungal chemicals used. The salivary isolates were more sensitive to fluoconazole compared to other groups (p < 0.05). Conclusion The prevalence of C. albicans in infected root canals of patients attending a dental hospital in UAE is low. The strains isolated were good biofilm formers, possessed strong phospholipase and haemolysin activity and were mostly of the genotype A. The fact that the root canal isolates possessed significant hydrolase activity, imply that they are equipped with virulence attributes necessary for survival within a harsh intracanal ecosystem.


Materials ◽  
2021 ◽  
Vol 14 (24) ◽  
pp. 7594
Author(s):  
Zhejun Wang ◽  
Ya Shen ◽  
Markus Haapasalo

Microbes are prevalent in the root canals of necrotic teeth, and they are the cause of primary and post-treatment apical periodontitis. Bacteria can dwell within the infected root canal system as surface-adherent biofilm structures, which exhibit high resistance to antimicrobial agents. Bioceramic materials, with their biocompatible nature and excellent physico-chemical properties, have been widely used in dental applications, including endodontics. This review focuses on the application of bioceramic technology in endodontic disinfection and the antibiofilm effects of endodontic bioceramic materials. Different bioceramic materials have shown different levels of antibiofilm effects. New supplements have emerged to potentially enhance the antibiofilm properties of bioceramics aiming to achieve the goal of microbial elimination in the root canal system.


Author(s):  
Aashima Puri ◽  
Jyoti Nagpal ◽  
Mayank Mall ◽  
Pinki Narwal ◽  
Deepti Garg ◽  
...  

Biofilm can be defined as a sessile multi-cellular microbial community characterized by cells that are firmly attached to a surface and enmeshed in a self produced matrix of extracellular polymeric substances.1,2 These are very prevalent in the apical root canals of teeth with primary and post-treatment apical periodontitis.3 It can be categorised as intracanal biofilms, extra radicular biofilms, periapical biofilms and biomaterial centered infections.4 The infected root canal harbors a polymicrobial population of aerobic, anaerobic, Gram-positive, and Gram-negative bacteria in a biofilm mode of growth. Bacterias involved are E. faecalis, Coagulase–negative Staphylococcus, S. aureus, Streptococci, P.aeruginosa, fungi, F. nucleatum, P. gingivalis, T. forsythensis, Actinomyces species and P. Propionicum. These are commonly isolated by culture, microscopy, immunological methods and molecular biology methods.3 The microbial communities grown in biofilm are remarkably difficult to eradicate with antimicrobial agents. Therefore, different antimicrobials ranging from antimicrobial irrigants to advanced antimicrobial methods such as lasers, photoactivated disinfection, and nanoparticles are employed in the management of infected root canal systems.4


2019 ◽  
Vol 53 (6) ◽  
Author(s):  
Cendranata Wibawa Ongkowijoyo ◽  
Adioro Soetojo

A male elderly patient came with buccal mucosa swelling associated with tooth 46 and 47. Periapical radiograph confirmed the presence of periapical lesion and narrow root canals on both teeth. Challenging impediments were sensed upon negotiating most of the narrow root canals which were common in elderly patients. Glide path creation involved pre-flaring, K-file bending, watch winding movement, and very gentle strokes. After achieving patency, shaping, cleaning, and sealing phase can be performed.


2020 ◽  
Vol 1 (12) ◽  
pp. 40-42
Author(s):  
F. Yu. Daurova ◽  
D. I. Tomaeva ◽  
S. V. Podkopaeva ◽  
Yu. A. Taptun

Relevance: the reason for the development of complications in endodontic treatment is poor-quality instrumental treatment root canals.Aims: a study of the animicrobial action and clinical efficacy of high-frequency monopolar diathermocoagulation in the treatment of chronic forms of pulpitis.Materials and methods: 102 patients with various chronic forms of pulpitis were divided into three groups of 34 patients each. In the first two groups, high-frequency monopolar diathermocoagulation was used in endodontic treatment in different modes. In the third group, endodontic treatment was carried out without the use of diathermocoagulation (comparison group). The root canal microflora in chronic pulpitis in vivo was studied twice-before and after diathermocoagulation.Results: it was established that high-frequency monopolar diathermocoagulation in the effect mode is 3, power is 4 (4.1 W) and effect is 4, power is 4 (5.4 W) with an exposure time of 3 seconds, it has a pronounced antibacterial effect on all presented pathogenic microflora obtained from the root canals of the teeth.


PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3583 ◽  
Author(s):  
Andreas Bartols ◽  
Bernt-Peter Robra ◽  
Winfried Walther

Background Reciproc instruments are the only contemporary root canal instruments where glide path preparation is no longer strictly demanded by the manufacturer. As the complete preparation of root canals is associated with success in endodontic treatment we wanted to assess the ability and find predictors for Reciproc instruments to reach full working length (RFWL) in root canals of maxillary molars in primary root canal treatment (1°RCTx) and retreatment (2°RCTx) cases. Methods This retrospective study evaluated 255 endodontic treatment cases of maxillary molars. 180 were 1°RCTx and 75 2°RCTx. All root canals were prepared with Reciproc instruments. The groups were compared and in a binary logistic regression model predictors for RFWL were evaluated. Results A total of 926 root canals were treated with Reciproc without glide path preparation. This was possible in 885 canals (95.6%). In 1°RCTx cases 625 of 649 (96.3%) canals were RFWL and in 2°RCTx cases 260 of 277 (93.9%). In second and third mesiobuccal canals (MB2/3) 90 out of 101 (89.1%) were RFWL with Reciproc in 1°RCTx and in the 2°RCTx treatment group 49 out of 51 cases (96.1%). In mesio-buccal (MB1) canals “2°RCTx” was identified as negative predictor for RFWL (OR 0.24 (CI [0.08–0.77])). In MB2/3 canals full working length was reached less often (OR 0.04 (CI [0.01–0.31])) if the tooth was constricted and more often if MB2/3 and MB1 canals were convergent (OR 4.60 (CI [1.07–19.61])). Discussion Using Reciproc instruments, the vast majority of root canals in primary treatment and retreatment cases can be prepared without glide path preparation.


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