Further Micro-scaled MEDI (Macronutrient Extraction and Determination from Invertebrates) v1

2021 ◽  
Author(s):  
Jordan Cuff
Keyword(s):  
Low Cost ◽  
Dry Mass ◽  
Sequential Process ◽  
Ecological Studies ◽  
Ecological Processes ◽  
Individual Level ◽  
Macronutrient Content ◽  
Colorimetric Assays ◽  
Cost Determination

Macronutrients, comprising carbohydrates, proteins and lipids, underpin many ecological processes, but their quantification in ecological studies is often inaccurate and laborious, requiring large investments of time and bulk samples, which make individual‐level studies impossible. This is a protocol for the direct, rapid and relatively low‐cost determination of macronutrient content from single small macroinvertebrates. Macronutrients are extracted by a sequential process of soaking in 1:12 chloroform:methanol solution to remove lipid and then solubilising tissue in 0.1 M NaOH. Proteins, carbohydrates and lipids were determined by colorimetric assays from the same individual specimens. Macronutrient Extraction and Determination from Invertebrates (MEDI) can directly and rapidly determine macronutrient content in tiny (dry mass <1 mg). Using MEDI, the total macronutrient content of over 50 macroinvertebrates can be determined within around 3 days of collection at a cost of ~$1.35 per sample.

scholarly journals Paper-based platforms with coulometric readout for ascorbic acid determination in fruit juices

The Analyst ◽  
2020 ◽  
Vol 145 (9) ◽  
pp. 3431-3439
Author(s):  
Estefanía Nunez-Bajo ◽  
M. Teresa Fernández-Abedul
Keyword(s):  
Ascorbic Acid ◽  
Low Cost ◽  
Fruit Juices ◽  
Ascorbic Acid Determination ◽  
Cost Determination ◽  
Acid Determination

Paper-based electrochemical platforms with coulometric readout are employed for fast and low cost determination of ascorbic acid in commercial juice samples.

scholarly journals Miniaturized Fluorimetric Method for Quantification of Zinc in Dry Dog Food

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Rute C. Martins ◽  
Ana M. Pereira ◽  
Elisabete Matos ◽  
Luisa Barreiros ◽  
António J. M. Fonseca ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Rapid Determination ◽  
Low Cost ◽  
Food Samples ◽  
Dog Food ◽  
Fluorimetric Assay ◽  
Relative Standard ◽  
The Stability ◽  
Cost Determination

Zinc is an essential trace element for animals in several biological processes, particularly in energy production, and it is acquired from food ingestion. In this context, a microplate-based fluorimetric assay was developed for simple, fast, and low-cost determination of zinc in pet food using 2,2′-((4-(2,7-difluoro-3,6-dihydroxy-4aH-xanthen-9-yl)-3-methoxyphenyl)azanediyl)diacetic acid (FluoZin-1) as fluorescent probe. Several aspects were studied, namely, the stability of the fluorescent product over time, the FluoZin-1 concentration, and the pH of reaction media. The developed methodology provided a limit of detection of 1 μg L−1 in sample acid digests, with a working range of 10 to 200 μg L−1, corresponding to 100–2000 mg of Zn per kg of dry dog food samples. Intraday repeatability and interday repeatability were assessed, with relative standard deviation values < 3.4% (100 μg L−1) and <11.7% (10 μg L−1). Sample analysis indicated that the proposed fluorimetric assay provided results consistent with ICP-MS analysis. These results demonstrated that the developed assay can be used for rapid determination of zinc in dry dog food.

A fast and low-cost method for determination of melamine in soil and sediment using high performance liquid chromatography

2014 ◽  
Vol 6 (12) ◽  
pp. 4124-4129 ◽  
Author(s):  
Yanfeng Nong ◽  
Xiaoguo Ma ◽  
Shaofeng Fan ◽  
Yumian Yu
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Low Cost ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction ◽  
Cost Determination ◽  
Soil And Sediment

Ultrasonic-assisted extraction with high performance liquid chromatography was used for rapid and low-cost determination of melamine in soil and sediment.

scholarly journals Analysis of 15N-NO3− Via Anoxic Slurries Coupled to MIMS Analysis: An Application to Estimate Nitrification by Burrowing Macrofauna

Water ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 2310
Author(s):  
Carpintero Moraes ◽  
Arroyave Gòmez ◽  
Vincenzi ◽  
Castaldelli ◽  
Fano ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Stable Isotope ◽  
Microbial Reduction ◽  
Ecological Studies ◽  
Anoxic Sediment ◽  
Ecological Processes ◽  
Membrane Inlet Mass Spectrometry ◽  
Wide Range ◽  
Analytical Approaches

The increasing use of the stable isotope 15N-NO3− for the quantification of ecological processes requires analytical approaches able to distinguish between labelled and unlabeled N forms. We present a method coupling anoxic sediment slurries and membrane inlet mass spectrometry to quantify dissolved 15N-NO3− and 14N-NO3−. The approach is based on the microbial reduction of 14N-NO3− and 15N-NO3− mixed pool, the determination of the produced 29N2 and 30N2, and the calculation of the original 15N-NO3− and 14N-NO3− concentrations. The reduction is carried out in 12 mL exetainers containing 2 mL of sediment and 10 mL of water, under anoxia. To validate this approach, we prepared multiple standard solutions containing 15N-NO3− alone or in combinations with 14N-NO3−, with final concentrations varying from 0.5 to 3000 µM. We recovered nearly 90% of the initial 14N-NO3− or 15N-NO3−, over a wide range of concentrations and isotope ratios in the standards. We applied this method to a 15N-NO3− dilution experiment targeting the measurement of nitrification in sediments with and without the burrower Sparganophilus tamesis. The oligochaete did not stimulate nitrification, likely due to limited ventilation and unfavorable conditions for nitrifiers growth. The proposed method is reliable, fast, and could be applied to multiple ecological studies.

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