The effects of sex, age and cigarette smoking on micronucleus and degenerative nuclear alteration frequencies in human buccal cells of healthy Bosnian subjects

Introduction: This study was performed to establish a baseline value of micronucleus frequency in buccal cells and to estimate the impact of the most common factors (sex and age, and smoking) on micronucleus and degenerative nuclear alteration frequencies in the sample of healthy Bosnian subjects.Methods: The Buccal Micronucleus Cytome (BMCyt) assay, based on scoring not only micronucleus frequency but also other genome damage markers, dead or degenerated cells, provides a measure of cytotoxic and genotoxic effects.Results: Our results showed the baseline buccal micronucleus frequency was 0.135% or 1.35‰, as well as positive correlations between micronucleus frequencies and formations of degenerative nuclear alterations (nuclear buds, karyolytic and karyorrhectic cells). The number of micronuclei in buccal cells was significantly higher in females than in males. There was positive association between the age and frequency of analysed cytogenetic biomarkers. Buccal cell micronuclei and degenerative nuclear alternations were more frequent among cigarette smokers than non-smokers and significantly higher in female smokers than in male smokers. Cytogenetic damages showed significantly positive correlation between intensity of smoking and the number of nuclear alterations. The years of smoking had a significant influence not only on the number of nuclear alterations but also in micronuclei and nuclear buds in buccal cells.Conclusions: The sex influences the number of micronuclei in human buccal cells. The ageing increased the number of micronuclei and other biomarkers of DNA damage. The cigarette smoking significantly increases the frequencies of micronuclei and nuclear buds, pyknotic, karyolytic and karyorrhectic cells.

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Exposure to Point-of-Sale Marketing of Cigarettes and E-Cigarettes as Predictors of Smoking Cessation Behaviors

Nicotine & Tobacco Research ◽

10.1093/ntr/ntx246 ◽

2017 ◽

Vol 21 (2) ◽

pp. 212-219 ◽

Cited By ~ 3

Author(s):

Dale S Mantey ◽

Keryn E Pasch ◽

Alexandra Loukas ◽

Cheryl L Perry

Keyword(s):

Smoking Cessation ◽

Cigarette Smoking ◽

Cue Reactivity ◽

Cigarette Smokers ◽

Point Of Sale ◽

Cigarette Advertising ◽

Advertising Exposure ◽

Cigarette Cessation ◽

Product Displays ◽

The Impact

Abstract Introduction Cue-reactivity theory suggests that smoking-related visual cues such as point-of-sale (POS) marketing (eg, advertising, product displays) may undermine cessation attempts by causing an increase in nicotine cravings among users. This study examined the relationship between recall of exposure to POS marketing and subsequent cessation behaviors among young adult cigarette smokers. Methods Participants included 813, 18–29 year old (m = 21.1, SD = 2.70), current cigarette smokers attending 24 Texas colleges. Multivariable logistic regression models examined the impact of baseline self-reported exposure to cigarette and e-cigarette advertising and product displays, on using e-cigarettes for cessation and successful cigarette cessation at 6-month follow-up. Two-way interactions between product-specific advertising and between product-specific displays were examined to determine if the marketing of one product strengthened the cue reactivity of the other. Baseline covariates included sociodemographic factors, past quit attempts, intentions to quit smoking, and nicotine dependence. Results Exposure to e-cigarette displays was associated with lower odds of cigarette smoking cessation, controlling for covariates and conventional cigarette display exposure. E-cigarette advertising was positively associated with the use of e-cigarettes for cigarette cessation among participants exposed to low (ie, at least 1 SD below the mean) levels of cigarette advertising. Cigarette advertising was associated with the use of e-cigarettes for cigarette cessation only among those exposed to low levels of e-cigarette advertising. Exposure to cigarette displays was not associated with either outcome. Conclusion Smoking-related cues at POS may undermine successful cigarette cessation. Exposure to product displays decrease odds of cessation. Advertising exposure increased odds for using e-cigarettes for cessation attempts, but may have guided smokers towards an unproven cessation aid. Implications By examining the interaction of conventional cigarette and e-cigarette marketing exposure, this study adds a unique insight into the impact of retail tobacco marketing on cigarette smoking cessation behavior among young adults. These findings suggest that policies that balance encouraging cigarette smoking cessation while limiting marketing strategies should be considered, such as POS product displays, that may undermine successful cessation attempts.

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The profile of menthol cigarette smokers in the months following the removal of these products from the market in England

10.21203/rs.3.rs-578480/v1 ◽

2021 ◽

Author(s):

Loren Kock ◽

Lion Shahab ◽

Ilze Bogdanovica ◽

Jamie Brown

Keyword(s):

Cigarette Smoking ◽

Age Groups ◽

Transitional Period ◽

Cross Sectional ◽

Cigarette Smokers ◽

Menthol Cigarette ◽

Menthol Cigarettes ◽

Chi Squared ◽

Cigarette Dependence ◽

The Impact

Abstract Introduction: In May 2020 the EU Tobacco Products Directive (TPD) ban on the sale of menthol cigarettes was implemented after a four-year transitional period. This study examined the prevalence of menthol cigarette smoking in the months following the ban, and according to sociodemographic and smoking characteristics.Methods: Cross-sectional data came from a representative survey of current smokers (18+) in England (unweighted n=1,577) during seven months (July-January) in 2020/2021. The weighted prevalence of menthol cigarette smoking as a proportion of total cigarette smoking was calculated, and chi-squared statistics assessed the relationship between menthol smoking, sociodemographic and smoking characteristics. Sources of purchase of menthol cigarettes were also explored.Results: The proportion of current smokers who smoked menthol cigarettes was similar in July to October 2020 (17.5%, 95% CI 15.3%-20.1%) compared with November 2020 to January 2021 (16.3%, 95% CI 13.9%-19.2%) (χ2(1)=0.38, P=0.56)). Menthol cigarette smoking was more common among younger age groups (16-24 = 29.0%; 25-34 = 23.3%) and women (21.1%). Menthol cigarette smokers showed lower cigarette dependence compared with other cigarette smokers. Purchases of menthol cigarettes from any illicit source in the past six months declined from 34.5% in July-October 2020 to 19.0% in November 2020–January 2021.Conclusions: A substantial proportion (just under a fifth) of current smokers in England reported menthol cigarette smoking between July-January 2020/2021 despite the ban. There was no decline in this proportion across the period, suggesting that smokers mitigated the impact of the ban by a variety of means, such as with legal menthol accessories.

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Waterpipe Smoking Is Associated with Changes in Fibrinogen, FVII, and FVIII Levels

Acta Haematologica ◽

10.1159/000492740 ◽

2018 ◽

Vol 140 (3) ◽

pp. 159-165 ◽

Cited By ~ 2

Author(s):

Abdel Rahim Mahmoud Muddathir ◽

Mozfar Idrees Abd Alla ◽

Omar F. Khabour

Keyword(s):

Cigarette Smoking ◽

Factor Viii ◽

Plasma Levels ◽

Coagulation Factors ◽

Factor Vii ◽

Waterpipe Smoking ◽

Cigarette Smokers ◽

Never Smokers ◽

The Impact ◽

Apparently Healthy

Cigarette smoking has been shown to be associated with changes in coagulation factors in the circulation and the subsequent thrombosis development. In this study, the impact of waterpipe smoking on the levels of fibrinogen, factor VII (FVII), and factor VIII (FVIII) was investigated. In addition, the effects of waterpipe smoking were compared to those of cigarette smoking and never smokers. A total of 80 male smokers (40 cigarette smokers and 40 waterpipe smokers) and 40 apparently healthy never smokers were recruited in the study. Both waterpipe smoking and cigarette smoking induced significant increases in the plasma levels of fibrinogen, factor VII, and factor VIII (p < 0.01). The magnitude of the increase in fibrinogen levels induced by waterpipe smoking was higher than that induced by cigarette smoking (p < 0.01), while similar increases were observed in other factors (p > 0.05). In addition, in the waterpipe group, the magnitude of the increase in fibrinogen and factor VIII was higher in the smokers with more than 3 years of use (p < 0.05). In conclusion, similar to cigarette smoking, waterpipe smoking modulates the levels of coagulation factors, suggesting its thrombotic potential.

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Death Anxiety: Extraversion, Neuroticism, and Cigarette Smoking

OMEGA - Journal of Death and Dying ◽

10.2190/wnku-jw13-8mfu-xxx5 ◽

1972 ◽

Vol 3 (1) ◽

pp. 53-56 ◽

Cited By ~ 27

Author(s):

Donald I. Templer

Keyword(s):

Cigarette Smoking ◽

Negative Correlation ◽

Death Anxiety ◽

Significant Negative Correlation ◽

Cigarette Smokers ◽

Eysenck Personality Inventory ◽

The Mean ◽

Smoking Group ◽

Positive Correlations ◽

Neuroticism Scale

There were significant positive correlations between the Death Anxiety Scale (DAS) and the Neuroticism Scale of the Eysenck Personality Inventory but not with the Extraversion Scale of this inventory. The mean DAS score of cigarette smokers was neither significantly different from that of non-smokers nor from that of ex-smokers. However, within the cigarette smoking group, there was a significant negative correlation (r = −.25) between DAS score and number of cigarettes smoked per day.

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Differences in Levels of Biomarkers of Potential Harm among Users of a Heat-not-burn Tobacco Product, Cigarette Smokers, and Never-Smokers in Japan: A Post-Marketing Observational Study

Nicotine & Tobacco Research ◽

10.1093/ntr/ntab014 ◽

2021 ◽

Author(s):

Chikako Sakaguchi ◽

Yasufumi Nagata ◽

Akira Kikuchi ◽

Yuki Takeshige ◽

Naoki Minami

Keyword(s):

Cigarette Smoking ◽

Tobacco Smoke ◽

Cigarette Smokers ◽

Potential Harm ◽

Actual Use ◽

Post Marketing ◽

Harmful Substances ◽

Never Smokers ◽

The Impact

Abstract Introduction Cigarette smoking is associated with the risk of certain diseases, but non-combustible products may lower these risks. The potential long-term health effects of the next-generation non-combustible products (heat-not-burn tobacco products (HNBP) or electronic vapor products) have not been thoroughly studied. The present study aimed to investigate the impact of biomarkers of potential harm (BoPH) of one of HNBP (a novel vapor product: NTV), under the conditions of actual use. Methods This study was an observational, cross-sectional, three-group, multi-center study. Exclusive NTV users (NTV, n = 259), conventional cigarette smokers (CC, n = 100) and never-smokers (NS, n=100) were enrolled. Biomarkers of tobacco smoke exposure (cotinine and total NNAL) and BoPH including parameters of physical pulmonary functions relevant to smoking-related diseases were examined, and subjects answered a questionnaire on cough-related symptoms (J-LCQ) and health-related quality of life (SF-36v2®). Results Levels of cotinine, total NNAL and BoPH (HDL-cholesterol, triglyceride, sICAM-1, WBC count, 11-DHTXB2, 2,3-d-TXB2, 8-epi-PGF2α, FEV1, %FEV1 and FEF25-75) were significantly different in the NTV group as compared to levels in CC group (p<0.05). Significantly higher levels of cotinine, total NNAL, and 2,3-d-TXB2, and lower levels of FEV1 and %FEV1, were observed among NVT users compared to the NS group. Conclusion In a post-marketing study under actual use conditions, BoPH associated with smoking-related disease examined in exclusive NTV users were found to be favorably different from those of CC smokers, a finding attributable to a reduction in exposure to harmful substances of tobacco smoke. Implications Cigarette smoking is associated with increased risk of pulmonary diseases like COPD, cardiovascular diseases, and certain cancers. There is a growing body of evidence that HNBP reduces the exposure associated with smoking and that there is a favorable change in BoPH. However, long-term effects regarding the relative health risks to HNBP users compared to CC smokers have not been examined. This study provides post-marketing data under actual use conditions of the effects on biomarkers of potential harm in NTV, one of HNBP, exclusive users compared to CC smokers and never-smokers. The evidence suggests that exclusive NTV users have favorable levels of BoPH compared to CC smokers, and that is result from a sustained reduction in exposure to harmful substances of tobacco smoke.

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The Effect of Cigarette Smoking on Blood and Biochemical Parameters: A Comparative Study Among Male Smokers and Non-Smokers In Baghdad City

Iraqi Journal of Science ◽

10.24996/ijs.2020.61.4.3 ◽

2020 ◽

pp. 727-731

Author(s):

Noor Sadiq Jaafar

Keyword(s):

Cigarette Smoking ◽

Biochemical Parameters ◽

Serum Bilirubin ◽

Blood Parameters ◽

Total Serum ◽

Cigarette Smokers ◽

Critical Problems ◽

Serum Biochemical ◽

Age Range ◽

The Impact

The impact of smoking on human health is remarkable and can lead to death. This research was performed to test the effects of cigarette smoking on some parameters that are considered as signs of critical problems in human body. The study was carried out on fifty Iraqi male smokers in Baghdad city, who smoked at least 10 cigarettes per day for at least 15 years. The group includes 25 male smokers with an age range of 20 -55 years and 25 male non-smokers who were collected with the same range of age for statistical comparison. The results of the study revealed significant increases in blood parameters, including hemoglobin (Hb, 16.0917 (g/dl) , packed cell volume (PCV, 49.2%), red blood cells (RBC, 5.4763 X1012/L), white blood cell (WBC, 12.5565 X109/L), and platelet (PLT, 430.000 x1012/L). Similar effects were observed in relation to the serum biochemical parameters o of kidney function (urea, 53.2400 mg/ dl; creatinine, 1.5480 mg/ dl) as well as liver function (alanine aminotransferase, ALT, 104.9200 U/l; aspartate transaminase, AST, 122.3040 U/l; alkaline phosphatase, ALP, 337.4000U/l); total serum bilirubin, TSB, 0.6780 mg/ dl). However, significantly decreased levels of total protein (60.6800 mg/ dl) and uric acid (4.2400 mg/ dl) were recorded in cigarette smokers when compared with non-smokers group.

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Subtle right ventricular dysfunction in asymptomatic chronic heavy cigarette smokers: a speckle tracking case-control study

The Egyptian Heart Journal ◽

10.1186/s43044-021-00151-y ◽

2021 ◽

Vol 73 (1) ◽

Author(s):

Mohammad Iqbal Janhangeer ◽

Ghada Youssef ◽

Weal El Naggar ◽

Dalia El Remisy

Keyword(s):

Cigarette Smoking ◽

Right Ventricular ◽

Ventricular Function ◽

Right Ventricular Function ◽

Speckle Tracking ◽

Longitudinal Strain ◽

Global Longitudinal Strain ◽

Cigarette Consumption ◽

Cigarette Smokers ◽

The Right

Abstract Background Chronic heavy cigarette smoking can affect the right ventriclular function. The standard echocardiography may not show early right ventricular functional changes, and a more sensitive measure is needed. The aim of this work was to evaluate the subtle subclinical effects of chronic heavy cigarette smoking on the right ventricular function. The study included 55 healthy asymptomatic chronic heavy cigarette smokers (smoking history of at least 5 pack-years and a daily cigarette consumption of at least 1 pack) and 35 healthy non-smoking control subjects. Patients underwent a full clinical assessment and a conventional as well as a 2D-speckle tracking transthoracic echocardiography of the right ventricle and data was compared between the 2 groups. Results The mean age was 32.9 ± 7.2 years in smokers and 30.9 ± 7.9 years in non-smokers (p = 0.227). The 2 groups showed comparable conventional right ventricular systolic and diastolic functions. Smokers showed a significantly lower (less negative) right ventricular global longitudinal strain (− 19.0 ± 3.2% vs. − 24.5 ± 3.5%, p < 0.001). Patients with a higher daily cigarette consumption showed a poorer right ventricular global longitudinal strain (p = 0.014). Conclusion Chronic heavy cigarette smoking can adversely affect the right ventricular function, a finding that can be easily missed by conventional echocardiography and can be better detected by the right ventricular speckle tracking.

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Does parental permissiveness toward cigarette smoking and alcohol use influence illicit drug use among adolescents? A longitudinal study in seven European countries

Social Psychiatry and Psychiatric Epidemiology ◽

10.1007/s00127-021-02118-5 ◽

2021 ◽

Author(s):

Emina Mehanović ◽

Federica Vigna-Taglianti ◽

Fabrizio Faggiano ◽

Maria Rosaria Galanti ◽

Barbara Zunino ◽

...

Keyword(s):

Alcohol Use ◽

Drug Use ◽

Cigarette Smoking ◽

Longitudinal Data ◽

Illicit Drug ◽

Large Population ◽

Illicit Drug Use ◽

European Countries ◽

The Impact

Abstract Purpose Adolescents’ perceptions of parental norms may influence their substance use. The relationship between parental norms toward cigarette and alcohol use, and the use of illicit substances among their adolescent children is not sufficiently investigated. The purpose of this study was to analyze this relationship, including gender differences, using longitudinal data from a large population-based study. Methods The present study analyzed longitudinal data from 3171 12- to 14-year-old students in 7 European countries allocated to the control arm of the European Drug Addiction Prevention trial. The impact of parental permissiveness toward cigarettes and alcohol use reported by the students at baseline on illicit drug use at 6-month follow-up was analyzed through multilevel logistic regression models, stratified by gender. Whether adolescents’ own use of cigarette and alcohol mediated the association between parental norms and illicit drug use was tested through mediation models. Results Parental permissive norms toward cigarette smoking and alcohol use at baseline predicted adolescents’ illicit drug use at follow-up. The association was stronger among boys than among girls and was mediated by adolescents’ own cigarette and alcohol use. Conclusion Perceived parental permissiveness toward the use of legal drugs predicted adolescents’ use of illicit drugs, especially among boys. Parents should be made aware of the importance of norm setting, and supported in conveying clear messages of disapproval of all substances.

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Heart Rate and Neurological Outcomes in Patients Undergoing Targeted Temperature Management

Journal of Intensive Care Medicine ◽

10.1177/0885066620982502 ◽

2020 ◽

pp. 088506662098250

Author(s):

Chad M. Conner ◽

William H. Perucki ◽

Andre Gabriel ◽

David M. O’Sullivan ◽

Antonio B. Fernandez

Keyword(s):

Heart Rate ◽

Positive Association ◽

Targeted Temperature Management ◽

Temperature Management ◽

Neurological Outcomes ◽

Neurologic Outcomes ◽

Resuscitation Guidelines ◽

The Mean ◽

The Impact ◽

The Relationship

Introduction: There is a paucity of data evaluating the impact of heart rate (HR) during Targeted Temperature Management (TTM) and neurologic outcomes. Current resuscitation guidelines do not specify a HR goal during TTM. We sought to determine the relationship between HR and neurologic outcomes in a single-center registry dataset. Methods: We retrospectively studied 432 consecutive patients who completed TTM (33°C) after cardiac arrest from 2008 to 2017. We evaluated the relationship between neurologic outcomes and HR during TTM. Pittsburgh Cerebral Performance Categories (CPC) at discharge were used to determine neurological recovery. Statistical analysis included chi square, Student’s t-test and Mann-Whitney U. A logistic regression model was created to evaluate the strength of contribution of selected variables on the outcome of interest. Results: Approximately 94,000 HR data points from 432 patients were retrospectively analyzed; the mean HR was 82.17 bpm over the duration of TTM. Favorable neurological outcomes were seen in 160 (37%) patients. The mean HR in the patients with a favorable outcome was lower than the mean HR of those with an unfavorable outcome (79.98 bpm vs 85.67 bpm p < 0.001). Patients with an average HR of 60-91 bpm were 2.4 times more likely to have a favorable neurological outcome compared to than HR’s < 60 or > 91 (odds ratio [OR] = 2.36, 95% confidence interval [CI] 1.61-3.46, p < 0.001). Specifically, mean HR’s in the 73-82 bpm range had the greatest rate of favorable outcomes (OR 3.56, 95% CI 1.95-6.50), p < 0.001. Administration of epinephrine, a history of diabetes mellitus and hypertension all were associated with worse neurological outcomes independent of HR. Conclusion: During TTM, mean HRs between 60-91 showed a positive association with favorable outcomes. It is unclear whether a specific HR should be targeted during TTM or if heart rates between 60-91 bpm might be a sign of less neurological damage.

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Patterns of non-cigarette tobacco and nicotine use among current cigarette smokers and recent quitters: Findings from the 2020 ITC Four Country Smoking and Vaping Survey

Nicotine & Tobacco Research ◽

10.1093/ntr/ntab040 ◽

2021 ◽

Author(s):

Lin Li ◽

Ron Borland ◽

K Michael Cummings ◽

Shannon Gravely ◽

Anne C K Quah ◽

...

Keyword(s):

Smoking Cessation ◽

Cigarette Smoking ◽

Smoking Status ◽

Tobacco Product ◽

Cigarette Tobacco ◽

Cigarette Smokers ◽

Tobacco Products ◽

Patterns Of Use ◽

Levels Of Use ◽

Current Cigarette

Abstract Introduction This study explores patterns of use of non-cigarette tobacco and nicotine products among adult cigarette smokers and recent ex-smokers. Along with cigarette smoking status we explore differences as a function of countries with different product regulations, gender and age. Methods Data came from the ITC Four Country Smoking and Vaping Wave 3 Survey conducted between February-June 2020. The analytic sample consisted of 9112 current cigarette smokers (at least monthly) and 1184 recent ex-smokers (quit cigarettes ≤ 2 years) from Australia, Canada, England, and the US. Respondents were asked about their cigarette smoking and current use of the following non-cigarette products: combustible tobacco (cigars, cigarillos, pipe, waterpipe); non-combustible tobacco (smokeless tobacco, and heated tobacco products (HTPs)); and non-tobacco nicotine products (nicotine vaping products (NVPs), nicotine replacement therapy (NRT), and nicotine pouches)). Results Overall, NVPs (13.7%) and NRT (10.9%) were the most reported nicotine products used, followed by cigars (5.3%), cigarillos (4.2%), and HTPs (3.5%). More than 21% current and recent ex-smokers of cigarettes reported using a non-tobacco nicotine product and non-combustible product, with respondents in England reporting the highest levels of use (>26%). Males, younger respondents, and current non-daily cigarette smokers were more likely to use non-cigarette nicotine products. Notably, 11.6% of ex-cigarette smokers were using other combustible tobacco. Conclusion Considerable percentages of current cigarette smokers and ex-smokers use non-cigarette nicotine products, and there are unexpectedly high levels of use of other combustible products by those recent ex-smokers of cigarettes which is concerning and has important implications for definitions of smoking cessation. Implications The tobacco product market has evolved to include new products which add to existing non-cigarette tobacco products creating a much more diverse nicotine market. This brief report provides a snapshot of use of various combustible and non-combustible nicotine containing products among current cigarette smokers and recent ex-smokers in four western countries. Our results indicate that use of non-cigarette tobacco and nicotine products among these cigarette smokers and recent ex-smokers is not low, particularly among males, younger and non-daily cigarette smokers. Use of other combustible tobacco among respondents that recently quit cigarette smoking is concerning and has important implications for definitions of smoking cessation. Increased emphasis on researching non-cigarette nicotine product use is warranted in tobacco control generally and smoking cessation in particular.

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