Development Of ELISA Kit For Detection Of Glyphosate-Resistant Genetically Modified Soybean

The goal of research was development of competitive ELISA kit for detection of genetically modified plants resistant to glyphosate. As the source for gene isolation we have used leaves of GM soybean RoundUp Ready, line 40-3-2 (Monsanto). Using polymerase chain reaction the gene encoding the enzyme 5-enolpiruvilshykimat-3-phosphate synthase (CP4 EPSPS) was isolated, which provides resistance to the herbicide glyphosate. Isolated gene has been cloned into plasmid vector pET24(a), creating genetic construct pET-CP4EPSPS. E.coli (BL 21(DE3)) has been transformed by genetic construct and over expressed after induction by 0.5 mM IPTG. Recombinant enzyme CP4 EPSPS with molecular weight 45 kD was purified as inclusion bodies and used as antigen for 96-well polystyrene plates immobilization. Monoclonal antibodies CP4 EPSPS mAb3 (Artron BioResearch Inc., Canada) was used for synthesis of horseradish peroxidase conjugate and receiving diagnostic reagent. We have shown that in the competitive ELISA we can detect at least 0.1% of GM plants resistant to glyphosate, on the example of soybean (line RR 40-3-2), moreover we can also detect rapeseed (line GT73) and sugar beet (line H7-1).

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Proteomics analysis reveals that foreign cp4-epsps gene regulates the levels of shikimate and branched pathways in genetically modified soybean line H06-698

GM crops & food ◽

10.1080/21645698.2021.2000320 ◽

2021 ◽

Vol 12 (1) ◽

pp. 497-508

Author(s):

Longguo Jin ◽

Daoping Wang ◽

Yongying Mu ◽

Yong Guo ◽

Yangjie Lin ◽

...

Keyword(s):

Genetically Modified ◽

Soybean Line ◽

Proteomics Analysis ◽

Genetically Modified Soybean ◽

Epsps Gene ◽

Cp4 Epsps Gene ◽

Cp4 Epsps

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Analysis of gm crops allowed for using in the european union

Naukovì dopovìdì Nacìonalʹnogo unìversitetu bìoresursiv ì prirodokoristuvannâ Ukraïni ◽

10.31548/dopovidi2021.05.004 ◽

2021 ◽

Author(s):

T. Tkachenko ◽

◽

V. Tsedyk ◽

V. Kornienko ◽

V. Ischenko ◽

...

Keyword(s):

European Union ◽

Raw Materials ◽

Genetically Modified ◽

Genetically Modified Crops ◽

Gm Crops ◽

The European Union ◽

Genetically Modified Soybean ◽

Gm Plants ◽

Tiny Amount ◽

Food And Feed

The development of biotechnology in the field of GMOs requires states to take specific decisions to regulate the spread of genetically modified crops. In the European Union all GM crops that circulation are subject to mandatory registration, which regulates the placing on the market and circulation of genetically modified raw materials, food and feed. The article presents systematized data about the registration of genetically modified soybean, maize and rapeseed in the European Union. It was established that most of the GM crops have introduced genes that give them tolerance to herbicides of different groups. The register of the European Union currently includes 12 events of soybean (GTS 40-3-2, A2704-12, Mon 89788, MON87705, DP 356043, A5547-127, FG 72, SYHTOH 2, DAS-44406-6, DAS-68416- 4, Mon 87708, BPS-CV127-9), 5 events of maize (MZHG0JG, DAS-40278-9, GA 21, NK 603, T 25), 3 events of rapeseed (GT 73, T45, TOPAS 19/2) with tolerance to herbicides. It has been shown that a significant number of registered GM plants have a combination of several events, including tolerance to herbicides and resistance to certain insects or improving quality features of crops. Among them are one event of soybean (DP305423-1), 9 events of maize (TC 1507, DP 4114-3, MON 87411-9, MON 87427, MON 88017, DAS59122-7, Bt 176, Bt 11, DAS 1507) and one event of rapeseed (MS8xRF3). Many GM crops (one event of soybean and 6 events of maize) have introduced genes that determine the plant's tolerance to insects. Only a tiny amount of GM crops are being with altered consumer or technological qualities. In the register of genetically modified crops, all events of GM crops are currently authorized for usage for food, supplements, feed and other product. А single event of maize (Mon 810), that was allowed for cultivation at the time of this analysis was at the stage of renewal of the permit.

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Changes of masometric indexs of internal organs rats in the exercised of native and genetically modified soybean in the composition of feed

Naukovij vìsnik Nacìonalʹnogo unìversitetu bìoresursìv ì prirodokoristuvannâ Ukraïni Serìâ Bìologìâ bìotehnologìâ ekologìâ ◽

10.31548/biologiya2018.287.045 ◽

2018 ◽

Vol 2018 (287) ◽

pp. 44-52

Author(s):

N. OMELCHENKO ◽

◽

H. DRONYK ◽

V. KUCHERIAVA ◽

◽

...

Keyword(s):

Genetically Modified ◽

Internal Organs ◽

Genetically Modified Soybean

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Faculty Opinions recommendation of Evidence for landscape-level, pollen-mediated gene flow from genetically modified creeping bentgrass with CP4 EPSPS as a marker.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1021834.244560 ◽

2004 ◽

Author(s):

Niklaus Ammann

Keyword(s):

Gene Flow ◽

Genetically Modified ◽

Creeping Bentgrass ◽

Landscape Level ◽

Cp4 Epsps

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Safety verification of genetically modified rice morphology, hereditary nature, and quality

Environmental Sciences Europe ◽

10.1186/s12302-021-00516-9 ◽

2021 ◽

Vol 33 (1) ◽

Author(s):

Dong Won Jeon ◽

Jae-Ryoung Park ◽

Yoon-Hee Jang ◽

Eun-Gyeong Kim ◽

Taehun Ryu ◽

...

Keyword(s):

Climate Change ◽

Gene Flow ◽

Negative Impact ◽

Genetically Modified ◽

Germination Rate ◽

Future Climate Change ◽

Yield Reduction ◽

Drought Tolerant ◽

Gm Plants ◽

Gm Rice

Abstract Background The drought environment occurs frequently due to the unpredictable future climate change, and drought has a direct negative impact on crops, such as yield reduction. Drought events are random, frequent, and persistent. Molecular breeding can be used to create drought-tolerant food crops, but the safety of genetically modified (GM) plants must be demonstrated before they can be adopted. In this research, the environmental risk of drought-tolerant GM rice was explored by assessing phenotype and gene flow. Drought resistance genes CaMsrB2 inserted HV8 and HV23 were used as GM rice to analyze the possibility of various agricultural traits and gene flow along with non-GM rice. Results When the traits 1000-grain weight, grain length/width, and yield, were compared with GM rice and non-GM rice, all agricultural traits of GM rice and non-GM rice were the same. In addition, when the germination rate, viviparous germination rate, pulling strength, and bending strength were compared to analyze the possibility of weediness, all characteristic values of GM rice and non-GM rice were the same. Protein, amylose, and moisture, the major nutritional elements of rice, were also the same. Conclusions The results of this research are that GM rice and non-GM rice were the same in all major agricultural traits except for the newly assigned characteristics, and no gene mobility occurred. Therefore, GM rice can be used as a means to solve the food problem in response to the unpredictable era of climate change in the future.

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