scholarly journals Effect of bromfenac on free radical oxidation in model systems

2019 ◽  
Vol 100 (4) ◽  
pp. 636-641 ◽  
Author(s):  
E F Galimova ◽  
Z G Khaibullina ◽  
D A Enikeev ◽  
Yu L Bortsova ◽  
K S Mochalov ◽  
...  

Aim. The study of free radical oxidation processes in an experiment on model systems using the anti-inflammatory drug bromfenac (nakwan) widely used in ophthalmology for the treatment of infectious and inflammatory diseases of the anterior chamber of the eye. Methods. The antioxidant capacity of the drug was evaluated by chemiluminescence registration and analysis of model systems that generate reactive oxygen species and reproduce lipid peroxidation processes using the chemiluminomer CL-003. The following parameters of spontaneous and induced chemiluminescence were determined: light sum and maximum luminescence amplitude, duration of latent period, amplitude of fast and slow flash. Results. When tested in vitro in two different model systems, a high antioxidant activity of the studied drug was established, up to complete suppression of chemiluminescence when 90 μg of bromfenac was added to the incubation medium, which characterizes the inhibition of the generation of reactive oxygen species. A significant increase in total antioxidant activity with bromfenac was also demonstrated, which is reflected by the integral parameter of chemiluminescence — light sum which decreased with the introduction of 10 μg of the drug by 1.2 times, and with 90 μg by 1.5 times. A comparative analysis of the antioxidant properties of various nonsteroidal anti-inflammatory drugs used in ophthalmic practice demonstrated a more pronounced efficacy of bromfenac compared to ketorolac, the use of which was not accompanied by statistically significant changes in chemiluminescence. A very important mechanism of the positive effect of bromfenac is the direct dependence of the action on its quantity in the reaction medium, which opens up prospects for the controlled correction of free radical phenomena and the excessive activation of lipid peroxidation in the imbalance of the pro- and antioxidant processes in biological systems. Conclusion. It is suggested that the protective effects of the drug in various infectious-inflammatory lesions of the eye can be determined, along with previously known properties, its antioxidant activity, restriction of increased production of reactive oxygen species and oxidative stress phenomena.

Nanoscale ◽  
2018 ◽  
Vol 10 (34) ◽  
pp. 15911-15917 ◽  
Author(s):  
Erik Jacques ◽  
Manuel Ahumada ◽  
Brianna Rector ◽  
Goonay Yousefalizadeh ◽  
Constanza Galaz-Araya ◽  
...  

Free radical oxidation of CLP-Trp peptides@AgNPs was studied using steady state & ultrafast spectroscopy and molecular dynamics.


Molecules ◽  
2020 ◽  
Vol 25 (14) ◽  
pp. 3244
Author(s):  
Katarzyna Naparło ◽  
Mirosław Soszyński ◽  
Grzegorz Bartosz ◽  
Izabela Sadowska-Bartosz

The inhibitory effects a range of synthetic and natural antioxidants on lipid peroxidation of egg yolk and erythrocyte membranes induced by a free radical generator 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH) was compared, with significant differences being found between both systems. When the protection by selected antioxidants against the effects of AAPH on erythrocytes (hemolysis, oxidation of hemoglobin and glutathione (GSH) and generation of reactive oxygen species (ROS)) was studied, most antioxidants were protective, but in some tests (oxidation of hemoglobin and GSH) some acted as prooxidants, inducing oxidation in the absence of AAPH and enhancing the AAPH-induced oxidation. These results demonstrate a diversified action of antioxidants in different systems and point to a need for careful extrapolation of any conclusions drawn from one parameter or experimental system to another.


Author(s):  
Dontha Sunitha

<p>ABSTRACT<br />To provide an outlook of the various available methods of antioxidant activity. Various available in vitro and in vivo methods are listed and the<br />procedure to perform the method, its mechanism is also explained in brief. 1,1-diphenyl-2-picrylhydrazyl method was found to be used mostly for the<br />in vitro antioxidant activity evaluation purpose while lipid peroxidation was found as mostly used in vivo antioxidant assay. An ethanol was with the<br />highest frequency as a solvent for extraction purpose. Summarized information on the various methods available provides with reliable information<br />to confirm the benefits of antioxidant effects.<br />Keywords: Antioxidant activity, Reactive oxygen species, Free radical, 1,1-diphenyl-2-picrylhydrazyl, Flavonoid.</p>


2020 ◽  
Vol 61 (3) ◽  
pp. 126-130
Author(s):  
Larisa V. Pilip ◽  
◽  
Olga V. Byakova ◽  
Maria E. Kazakova ◽  
◽  
...  

The chemical processes occurring in the animal body under pathological conditions are of interest to researchers. The objective of the research was to study the indicators of lipid peroxidation and antioxidant activity in horses infested with helminthiases and treated with anthelmintic paste with an antioxidant. The study has proved the role of parasitic infestation in accelerating the processes of free radical oxidation, which is manifested by a decrease in the light sum of radicals (36.62-39.21), the maximum luminous intensity (6.13-6.98) and antioxidant activity (5.65-6.2) at the start of the research. Chronic invasion increases the concentration of under-oxidized products up to 38.59, and the rate of initiating free radical oxidation – up to 6.95, the values of antioxidant activity being stably low (5.51-5.91). An important role in the regulation of lipid peroxidation in horses during helminthiases, as well as in the process of deworming, is played by the antioxidants contained in anthelmintic preparations. The antioxidant santohin included as a compound in the «Alezan» preparation suppresses enhanced peroxidation and activates the antioxidant protection of the body, which is reflected in a decrease in the light sum of the radicals to as low as 26.73, a maximum luminous intensity to – 3.76, and an increase in antioxidant activity – up to 7.16.


Author(s):  
І.Б. Івануса

<p>FEATURES OF FREE RADICAL OXIDATION AND ANTIOXIDANT SYSTEM PARAMETRS OF ANIMALS AT ACUTE TOXIC AND SUBCHRONIC POISONING BY ACETAMINOPHEN WITH PROLONGED USAGE OF ESTROGEN AND PROGESTIN AT THE CORRECTION BY THIOTRIAZOLINE AND HEPADIF</p><p align="center">I.B. Ivanusa</p><p align="center">TernopilStateMedicalUniversity</p><p> </p><p>Activation of lipid peroxidation of animals at poisoning by acetaminophen with prolonged usage of estrogen and progestin are investigated. This is evidenced by increased concentration of reactive oxygen species by mononuclear leukocytes, the content of lipid peroxidation products: malondialdehyde, diene and triene conjugates. The ability of the corrective factors inhibits the formation of these products in the plasma and homogenate can indirectly evidenced about the antioxidant properties of the drug. So it is interested to investigate the influence of thiotriazoline and hepadif on activity of these processes in rats, which are poisoned by acetaminophen with prolonged usage of estrogen and progestin.</p><p> </p><p>KEY WORDS: acetaminophen, thiotriazoline, hepadif, free radical oxidation of lipids and proteins, antioxidant system.</p><p>INTRODUCTION. Acetaminophen is present in more than 200 drugs at various brand names. Almost all of them are used as analgesic and antipyretics. Poisoning may be at usages a big single dose of pure drug or combined drugs containing acetaminophen. Hepatoprotectors are one of the most widely using drugs for the treatment of liver diseases. These drugs are used for metabolism normalization, increase resistance to pathogenic factors, normalization of functional activity and stimulation of reparative - regenerative processes in the liver.</p><p>Therefore, we set a goal to investigate the effect of acetaminophen on parameters of free radical oxidation and antioxidant system of animals at poisoning by acetaminophen with prolonged usage of estrogen and progestin at the correction by Thiotriazoline and Hepadif.</p><p>INVESTIGATION METHODS. The experiments were performed on white female rats weighing 200 ±20 g, are kept on a standard diet of vivarium and free access to water.</p><p>We did three series of experiments. In the first experiment: toxic damage of rats caused by a single intragastric administration of acetaminophen suspension in 2% starch solution at a dose of 1250 mg/kg body weight (1/2 LD<sub>50</sub>), the second - a suspension of acetaminophen in 2% starch solution at a dose of 55 mg/kg (highest therapeutic dose) is administered within 7 days. Levonorgestrel in 2% solution of starch is administered both series intragastric at a dose of 1.17 mg/kg, and ethinylestradiol - a dose of 0.23 mg/kg within 40 days. Thiotriazoline was injected intraperitoneally at a dose of 100 mg/kg; Hepadyf - intraperitoneally at a dose of 8.6 mg/kg.</p><p>In 1 series of experiments, the experimental rats were divided into 3 groups: 1st - intact (control); 2nd – poisoning by acetaminophen after 40-day administration of levonorgestrel and ethinylestradiol, 3rd - poisoning by acetaminophen after 40-days administration of levonorgestrel, ethinylestradiol and once Thiotriazoline administration.</p><p>In 2 series of experiments, the experimental rats were divided into 3 groups: 1st - intact (control); 2nd - rats with acetaminophen administration within 7 days after 40-day administration of levonorgestrel and ethinylestradiol, 3rd - poisoning by acetaminophen after 40-days administration of levonorgestrel, ethinylestradiol and 7-days Thiotriazoline administration.</p><p>In 3 series of experiments, the experimental rats were divided into 3 groups: 1st - intact (control); 2nd - rats with acetaminophen administration within 7 days after 40-day administration of levonorgestrel and ethinylestradiol, 3rd - poisoning by acetaminophen after 40-days administration of levonorgestrel, ethinylestradiol and 7-days Hepadif administration.</p><p>RESULTS AND DISCUSSION. Concentration of diene conjugates decreased in blood plasma of rats with single and 7-days administration of acetaminophen after 40-days administration of estrogen and progestin and Thiotriazoline administration. Decrease intensity generation of reactive oxygen by blood mononuclear leukocytes is observed at Hepadif action, and Hepadif almost neutralized its toxic effect at seven-times administration of acetaminophen at therapeutic doses. Concentration diene (respectively 2.8 and 2.6 times), and triene conjugates (3.7 and 3.2 times) decreased in plasma of affected animals. Similarly effect was in liver homogenate.</p><p>Amount of oxidized modified proteins in blood plasma and liver of affected animals decreased at correction of lesion by Thiotriazoline and Hepadif. The administration of corrective drugs is caused decrease of malonic aldehyde concentration in plasma and liver of affected rats. Peroxidase activity in blood at correction by Thiotriazoline decreased respectively on 159 and 162% compared with affected animals. Greater effect is observed at action of Hepadif, administration of which normalizated of the peroxidase activity of blood and concentration of ceruloplasmin. Administration of Thiotriazoline and Hepadif has protective effects on glutathione system.</p><p>CONCLUSIONS. Usage Thiotriazoline and Hepadif in rats with acetaminophen toxic damage against prolonged administration of estrogens and progestins inhibit reactive oxygen species formation by mononuclear leukocytes, decreas intensity of lipid peroxidation and protein, and normalization of enzyme and nonenzyme links of antioxidant system.</p>


Author(s):  
O. M. Larycheva

The experiments on male rats demonstrated that under the conditions of pleurisy induced by carrageenan and short melatonin deficit, a significant increase in the level of reactive oxygen species and products of lipid peroxidation in the lungs takes place.


2014 ◽  
Vol 12 (4) ◽  
pp. 13-21 ◽  
Author(s):  
Vasiliy Egorovich Novikov ◽  
Olga Sergeevna Levchenkova ◽  
Yelena Vasilyevna Pozhilova

This review is devoted to analysis of modern scientific investigations about role of reactive oxygen species (ROS) in physiology and pathophysiology of cell. Key issues of ROS generation, their function in signaling pathways, role in the cellular pathology, functioning of antioxidant system are discussed in the paper. The review also summarizes literature data about possibility of pharmacological regulation of the activity of free radical oxidation reactions, which have practical value for effective pharmacotherapy of diseases in pathogenesis of which destructive action of ROS is present.


2021 ◽  
Vol 6 (3) ◽  
pp. 8-14
Author(s):  
A. K. Martusevich ◽  
A. G. Galka ◽  
E. S. Golygina ◽  
A. S. Fedotova

Aim. To assess the effects of reactive oxygen species and nitric oxide on the scar tissue ex vivo.Materials and Methods. The study was performed using fragments of scar tissue (n = 10) removed intraoperatively in patients with Dupuytren's contracture. Each fragment was divided into 3 equal segments: 1) control; 2) treated with singlet oxygen for 5 minutes; 3) treated with nitric oxide (20 ppm) for 5 minutes. Upon the indicated treatment, we evaluated the dielectric properties of the tissue employing nearfield resonant microwave sensing using a customised software package (Institute of Applied Physics, Russian Academy of Sciences). Then, each segment was homogenised and the parameters of oxidative metabolism (intensity of free radical oxidation and total antioxidant activity) were measured in homogenates by Fe-induced biochemiluminescence.Results. Treatment of scar tissue fragments by singlet oxygen and nitric oxide altered the dielectric properties of the tissue and the intensity of free radical oxidation. Singlet oxygen action moderately increased the dielectric permittivity of the tissue and rendered a balanced stimulating effect on proand antioxidant systems. Nitric oxide significantly augmented dielectric permittivity and conductivity and raised the antioxidant potential of the tissue.


Antioxidants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 388
Author(s):  
Xiao Dan Hui ◽  
Gang Wu ◽  
Duo Han ◽  
Xi Gong ◽  
Xi Yang Wu ◽  
...  

In this study, blueberry and blackcurrant powder were chosen as the phenolic-rich enrichments for oat bran. A Rapid Visco Analyser was used to form blueberry and blackcurrant enriched oat pastes. An in vitro digestion process evaluated the changes of phenolic compounds and the in vitro antioxidant potential of extracts of pastes. The anthocyanidin profiles in the extracts were characterised by the pH differential method. The results showed that blueberry and blackcurrant powder significantly increased the content of phenolic compounds and the in vitro antioxidant capacity of pastes, while the total flavonoid content decreased after digestion compared to the undigested samples. Strong correlations between these bioactive compounds and antioxidant values were observed. Lipopolysaccharide-stimulated RAW264.7 macrophages were used to investigate the intracellular antioxidant activity of the extracts from the digested oat bran paste with 25% enrichment of blueberry or blackcurrant powder. The results indicated that the extracts of digested pastes prevented the macrophages from experiencing lipopolysaccharide (LPS)-stimulated intracellular reactive oxygen species accumulation, mainly by the Kelch-like ECH-associated protein 1 (Keap1)/nuclear factor erythroid 2-related factor 2 (Nrf2) signalling pathway. These findings suggest that the bioactive ingredients from blueberry and blackcurrant powder enhanced the in vitro and intracellular antioxidant capacity of oat bran pastes, and these enriched pastes have the potential to be utilised in the development of the functional foods.


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