scholarly journals About changes in peripheral blood in sporadic typhus

2021 ◽  
Vol 43 (3) ◽  
pp. 75-75
Author(s):  
Z. V. Kryukova
Keyword(s):  
Peripheral Blood ◽  
Leukocyte Count ◽  
Agglutination Reaction

For a number of years, we have observed patients with sporadic typhus, in whom the number of erythrocytes, leukocytes, platelets, leukocyte count, ROE and Waldman's can test were examined at least 3 times in dynamics. The diagnosis in all was confirmed by a positive agglutination reaction with Provacek's rickettsia or by binding of complement with an antigen from Provachek's rickettsia in titers 1: 40-1: 1280.

Outcomes of Peripheral Blood Eosinophilia in Hospitalized Exacerbations of COPD

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Samar El Sharkawy ◽  
Riham Hazem Raafat ◽  
Reem Osama Mohamed Ahmed Qassem
Keyword(s):  
Peripheral Blood ◽  
Diagnostic Performance ◽  
Leukocyte Count ◽  
Airflow Limitation ◽  
Total Leukocyte Count ◽  
Blood Eosinophil ◽  
Copd Exacerbation ◽  
Peripheral Blood Eosinophil ◽  
Copd Exacerbations ◽  
Cutoff Points

Abstract Background The Global Initiative for Chronic Obstructive Lung Disease (GOLD) guidelines define COPD as a disease state characterized by airflow limitation that is not fully reversible, is usually progressive, and is associated with an abnormal inflammatory response of the lungs to inhaled noxious particles or gases. Objective To identify outcomes of patients with eosinophilic COPD exacerbations requiring hospital admission. Patients and Methods This study is a prospective cohort study that was conducted on two groups of total 60 patients recruited from Ain Shams University hospitals between October 2019 and July 2020. Group 1: Eosinophilic COPD exacerbation if the peripheral blood eosinophil on admission is ≥ 200 cells/µL and/or ≥2% of the total leukocyte count Group 2: Non-eosinophilic COPD exacerbation if the peripheral blood eosinophil on admission is < 200 cells/µL and/or < 2% of the total leukocyte count. Results There was significant high diagnostic performance in predicting readmission at 6-month among eosinophilic group. Eosinophils count, percent (%) and NLR cutoff points had high characteristics (highest in NLR ≥3.1 at discharge) in predicting readmission at 6-month among eosinophilic group. Diagnostic performance of Eosinophils count, percent (%) and NLR were assessed. Eosinophils count, percent (%) and NLR had significant high diagnostic performance in predicting readmission at 6-month among eosinophilic group. Eosinophils count, % and NLR cutoff points had high characteristics (highest in NLR ≥2.1 at discharge) in predicting readmission at 6month among non-eosinophilic group. Conclusion Eosinophils can be used as a prognostic marker in non-infective COPD exacerbations. Validity of eosinophil count and percent as a prognostic parameter in COPD exacerbation can be increased by combining with other parameters for example NLR.

scholarly journals Immunostimulatory properties of bigroot geranium (Geranium macrorrhizum L.) extract

Medicina ◽  
2007 ◽  
Vol 43 (1) ◽  
pp. 60 ◽  
Author(s):  
Vilma Jurkštienė ◽  
Anatolijus Kondrotas ◽  
Egidijus Kėvelaitis
Keyword(s):  
T Lymphocytes ◽  
B Lymphocytes ◽  
Peripheral Blood ◽  
Leukocyte Count ◽  
Ethanol Extract ◽  
Food Supplement ◽  
Total Leukocyte Count ◽  
Control Group ◽  
Case Group ◽  
Cell Counts

The aim of the study was to investigate the immunostimulatory properties of bigroot geranium. Material and methods. Possible nonspecific characteristics of bigroot geranium were evaluated by the total leukocyte count in the peripheral blood, and qualitative changes of blood were assessed using Shilling’s formula by evaluating changes in lymphocyte counts. In addition, we also studied changes in the counts of Tcell precursors in the thymus and B lymphocytes in the spleen. Ethanol extract of the leaves of bigroot geranium was produced at the Department of Food Technology, Kaunas University of Technology. Studies were performed on mice Bl 57 (n=21). The control group (n=7) received distilled water at a dose of 1 mL/day. The second and third groups received 1% and 10% extract of bigroot geranium, respectively, as a food supplement. Changes in cell counts were investigated after 4 weeks following the initiation of the trial. Results. After a 4-week administration of 1% extract of bigroot geranium (1 mL/day) (mice group, n=7), leukocyte count in the peripheral blood increased to 6.1×109 cells/L, and lymphocyte count – to 70%, but changes were not statistically significant. The other case group of mice (n=7) received 10% extract of bigroot geranium for 4 weeks at a dose of 1 mL/day. In this group, leukocyte count in the peripheral blood increased statistically significantly from 4.4×109 cells/L to 7.2×109 cells/L (p<0.01), and lymphocyte percentage – from 52% to 80% (p<0.001), as compared to control. Thymocyte (T lymphocytes) counts in thymus and splenocyte (B lymphocytes) counts in the spleen showed a tendency to increase after the administration of 1% and 10% extracts. After a 4-week administration of 1% extract of bigroot geranium, thymocyte and splenocyte counts increased from 0.342×106 cells to 0.372×106 cells per mg of tissue and from 0.395×106 cells to 0.405×106 cells per mg of tissue, respectively, as compared to control group (p>0.1). After the administration of 10% extract of bigroot geranium, thymocyte count increased to 0.488×106 cells per mg of tissue (p<0.01), and splenocyte count – to 0.504×106 cells per mg of tissue (p<0.01). Conclusion. The extracts of the leaves of bigroot geranium increased leukocyte count and lymphocyte percentage in the peripheral blood, and after a 4-week administration of 10% extract of bigroot geranium, a statistically significant increase in the counts of T lymphocytes (in the thymus) and B lymphocytes (in the spleen) was observed. The immunostimulatory effect depends on the dose of the extract.

Linear Relationship Between Lymphocyte Subpopulations Counts in Peripheral Blood and Buffy-Coat during Extracorporeal Photopheresis in Patients with Graft-Versus-Hot-Disease Using an Off-Line System

Blood ◽  
2014 ◽  
Vol 124 (21) ◽  
pp. 5826-5826
Author(s):  
Cristina Pascual ◽  
Mari­a Jose Penalva ◽  
Ana Pérez-Corral ◽  
Laura Solán ◽  
Sara Redondo ◽  
...  
Keyword(s):  
Linear Relationship ◽  
Peripheral Blood ◽  
Conflicts Of Interest ◽  
Leukocyte Count ◽  
Surrogate Marker ◽  
Buffy Coat ◽  
Lymphocyte Subpopulations ◽  
Extracorporeal Photopheresis ◽  
Line System ◽  
The Mean

Abstract Introduction: Extracorporeal photopheresis (ECP) is a treatment modality that entails leukapheresis followed by mixing the buffy coat with 8-methoxypsoralen (8-MOP) and exposing it to UVA light. The buffy coat is then returned to the patient. ECP may be performed employing two different techniques: an on-line and off-line procedure. The off-line system includes two steps: a processing of buffy coat before reinfused to the patient. The treatment is thought to have an immunomodulatory effect and is most commonly used to treat cutaneous T cell lymphoma, acute and chronic graft-versus-host disease (GVHD), and heart and lung allograft rejection. The exact mechanism and optimal cells dose to be treated is unknown. At the present time, a standard protocol is used generally without considering peripheral blood (PB) leukocytes counts or lymphocyte subpopulations (LP) of the patient. With the off-line system, PB leukocytes counts and LP analysis may be useful to choose the amount and distribution of cells to be infused. The first objective of this study is to examine the quantitative correlation between PB LP and the buffy-coat in order to set individualized guidelines of treatment. Once this relationship is understood, the PB LP may serve as a surrogate marker for cell dose treated and help predicting the efficiency of ECP. The second objetive of this study is to examine the mean performance of the buffy coat LP categorized according to PB leukocytes counts (<1.5 x 109/L and >1.5x 109/L). Patients and methods: Twenty two consecutive patients with refractory GVHD were prospectively studied, from november 2009 to may 2014. Apheresis procedures were perfomed with COBE Spectra system (Terumo BCT®, Lakewood, CO, USA; version 7.0) by processing 1.5-2 times the patient blood volume. The product was transferred to a UVA-permeable bag (UVA, Macopharma, France), added 5 mL (0.1 mg) of 8-methoxypsoralen (8-MOP) aqueous solution (S.A.L.F.®, Cenate Sotto, Italy), exposed to UVA irradiation (Macogenic G2, Macopharma®), and then reinfused. Peripheral blood sample was drawn before ECP. Just before reinfused, buffy coat sample was drawn. Spearman correlation analysis was performed between the LP CD3+CD4+, CD3+CD8+, CD19+, NK of the preapheresis peripheral blood patient and the buffy coat infused analyzed by multiparameter flow cytometry 5 colors (FC500-Beckman Coulter®) in the total sample and in three groups according to preapheresis leukocytes counts (<2.5, 2.5-7.5, >7.5x 109/L). The mean performance was calculated: CD3+CD4+, CD3+CD8+, CD19+, NK+ LP count in buffy coat/ CD3+CD4+, CD3+CD8+, CD19+, NK+ PB /ml x treatment volume (ml) x 100. The mean performance of the buffy coat LP categorized according to preapheresis leukocytes counts (<1.5x 109/L and >1.5 109/L) were compared by Mann-Whitney test. Results: A total of 22 patients and 136 procedures were included in the final analysis. CD3+CD4+, CD3+CD8+, CD19+, NK LP in peripheral blood significantly correlated with those in buffy coat collected by COBE Spectra system, r= 0,74, 0.78, 0,92, 0,39 respectively (table 1). The LP mean doses and mean performance in buffy coat infused are specified in Table 1. The correlations was stronger in all LP with PB leukocytes counts <2.5 x 109/L (table2). We have not found any statistical correlation between the performance of the LP CD3+CD4+, CD3+CD8+, CD19+, NK according to PB leukocytes counts (<1.5x109/L and >1.5x109/L). Conclusions: The buffy coat contains great variability in lymphocyte subpopulations with predominant levels of CD3+CD8+. There is a robust linear relationship between all PB and buffy coat LP. The mean performance LP was around 40% and it was not related to very low PB leukocyte count (<1.5x109/L). The correlation was stronger with lower leukocytes counts in PB. If we could demonstrate a relationship between cell doses infused and clinical response, we could plan the necessary dose for each patient according to the PB leukocyte count and LP preapheresis. Figure 1 Figure 1. Figure 2 Figure 2. Disclosures No relevant conflicts of interest to declare.

scholarly journals HASIL HITUNG NORMOBLAS ANTARA SEDIAAN HAPUSAN DARAH TEPI PENDERITA AML DENGAN ALL

2018 ◽  
Vol 16 (1) ◽  
pp. 39
Author(s):  
Hidayat . ◽  
Nina Susana Dewi ◽  
Nadjwa Zamalek Dalimoenthe
Keyword(s):  
Peripheral Blood ◽  
Leukocyte Count ◽  
Lymphoblastic Leukemia ◽  
Clinical Pathology ◽  
Mean Value ◽  
Blood Smears ◽  
Immature Form ◽  
The Subject ◽  
The Mean ◽  
Peripheral Blood Smears

Normoblast is an immature form of erythrocyte in erythropoietin system. Normally, normoblast can be found in peripheral blood healthy neonates. The existence of normoblast in peripheral blood might be the sign of pathologic conditions such as hemolytic anemia,acute blood loss, and ischemia and bone marrows abnormalities like malignancy or leukemia. In acute leukemia (Acute MyeloblasticLeukemia and Acute Lymphoblastic Leukemia), normoblast existence in peripheral blood may due to erythropoietin system suppression.The aim of this study is to compare normoblast count between AML and ALL, and also to find out the correlation between leukocyte andnormoblast count in AML and ALL. The subject of this study were patient diagnosed as AML (30) and ALL (30) in Hematology Divisionof Clinical Pathology Department at Dr.Hasan Sadikin Hospital Bandung in July 2006–August 2008. In this study we examined 30peripheral blood smears from AML and 30 peripheral blood smears from ALL. Leukocyte count result was derived from CBC performedwith Sysmex KX-21. The mean value of normoblast count from AML blood smear patients is 1930.60 (3.60/100 WBC) while ALL bloodsmear patients is 309.60 (0.43/100 WBC). Statistically this difference is significant (p < 0.001). There are strong correlation betweenleukocyte count and normoblast count within both group (r = 0.851, r = 0.948; p < 0.001).

scholarly journals The Effects of Triethylene Melamine and Related Compounds on the Leukocytes of Mouse Leukemia

Blood ◽  
1951 ◽  
Vol 6 (6) ◽  
pp. 504-512 ◽  
Author(s):  
JOSEPH H. BURCHENAL ◽  
LORRAINE F. WEBBER ◽  
JUNE L. BIEDLER ◽  
G. MARIE MEIGS ◽  
GODFREY D. STOBBE
Keyword(s):  
Bone Marrow ◽  
Survival Time ◽  
Peripheral Blood ◽  
Leukocyte Count ◽  
Total Leukocyte Count ◽  
Therapeutic Dosage ◽  
Mouse Leukemia ◽  
Massive Dose ◽  
Rapid Fall ◽  
Related Compounds

Abstract 1. In the usual therapeutic dosage in mice with the Ak4 strain of leukemia, TEM caused a prolongation of survival time, held the total leukocyte count down at severely leukopenic levels, delayed the appearance of immature lymphocytes in the peripheral blood, and caused a marked delay in the appearance of leukemic infiltrations of the liver, spleen, bone marrow and kidney. 2. A single massive dose of TEM, administered on the seventh day of the disease when the total leukocyte count was high, caused a rapid fall in the count and depressed the number of prolymphocytes in the peripheral blood. 3. Single massive doses of such structurally related compounds as hexamethylene diethylenurea, 2,4-diethylenimino-6-amino-s-triazine, and to a lesser degree, 2-ethylenimino-4,6-dimethoxy-s-triazine caused a similar fall in the total leukocyte count when administered on the seventh day of the disease.

scholarly journals The Relative Biologic Effectiveness of 200 KV and 23.5 MEV (Beatatron) X-Radiation

Blood ◽  
1952 ◽  
Vol 7 (8) ◽  
pp. 826-835 ◽  
Author(s):  
T. P. TING ◽  
H. E. JOHNS ◽  
L. B. JAQUES
Keyword(s):  
Single Dose ◽  
Peripheral Blood ◽  
Leukocyte Count ◽  
Whole Body ◽  
Similar Dose ◽  
Different Strains ◽  
Relative Biologic Effectiveness

Abstract 1. The leukocyte value in the peripheral blood of three different strains of mice was followed after a single dose of whole body x-radiation. The maximum leukocyte depression was used as a basis for comparing the biologic effectiveness of the betatron radiation and the 200 KV x-radiation. 2. The ratio of effectiveness in producing 70 per cent leukocyte reduction, for betatron radiation to 200 KV x-radiation, for the three strains was 0.65. 3. The onset of recovery from leukopenia for equal doses of radiation occurred earlier in animals treated with betatron radiation than in those treated with a similar dose of 200 KV x-radiation. 4. The degree of leukopenia and delay in recovery was found to increase somewhat with age of animals treated with 200 KV x-radiation and to a less extent in animals treated with betatron radiation. 5. At a dose of 600 r, over 90 per cent of the animals treated with 200 KV died within a few days, while none of the animals exposed to the same dose of betatron radiation died in the same period. The leukocyte count immediately before death was about 2 per cent of control values. 6. For a dose of 600 r, the lymphocytes were equally reduced by 200 KV and betatron radiation, while the granulocytes were much less reduced by betatron than by 200 KV radiation.

Peripheral Blood HPC Counts Are Augmented in CML Patients with Cytogenetic Findings of Disease as Compared to Patients in Cytogenetic Remission.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 4691-4691
Author(s):  
Ernesto de Meis ◽  
Rita C.B. Morais ◽  
Luize O. Carvalho ◽  
Arthur Moellmann-Coelho ◽  
Maria M.K. Martinez ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Myeloid Leukemia ◽  
Leukocyte Count ◽  
Philadelphia Chromosome ◽  
Cell Disease ◽  
Differential Leukocyte Count ◽  
Non Invasive ◽  
Cd34 Cells ◽  
High Prevalence ◽  
Cytogenetic Remission

Abstract Chronic Myeloid Leukemia (CLM) is a stem cell disease characterized by inhibition of apoptosis and increased proliferation of bone marrow (BM) compounds. Evaluation of hematopoetic precursors cells on the peripheral blood (PB) of these patients show a high prevalence of leukemic CD34+ cells. Recently it was demonstrated that measurement of HPC by IMI channel on Sysmex showed an acceptable correlation between Hematopoetic Progenitors CD34+ cells counts. The aim of this work was to compare precursor cells (HPC) numbers in the PB of CML patients with disease and under remission. For this we studied 61 CML patients that had been treated with different protocols. From these 22 were in cytogenetic remission but 39 still presented more than 5% BM cells with Philadelphia chromosome (Ph+). Although the normal levels of CD34+ HPC in PB is considered to be very low, we considered less than 0.05% of leukocytes as normality. HPC in PB were counted using an automated peripheral blood counter (Sysmex XE-2100). We found that Ph+ CML patients had a significant increased number of PB HPC compared to CML patients under remission (with a confidence interval of 95%, and a corrected Yates analyses with X2 16.78 and p<0.0001). The increase in PB HPC was not related with the number of white cells counts at moment of the exam. These findings suggest that the presence of a high number of PB HPC in CML can be used as a non-invasive marker of the disease, more reliable than total and differential leukocyte count.

scholarly journals Prevalence of Allergic and Non-allergic Asthma in Kumaun Region of Uttarakhand, India: A Cross-sectional Study

2020 ◽  
Author(s):  
Poonam Kumari ◽  
Ram Gopal Nautiyal ◽  
HK Thakar
Keyword(s):  
Blood Cell ◽  
Allergic Asthma ◽  
Peripheral Blood ◽  
Eosinophil Count ◽  
Leukocyte Count ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Asthma Patients ◽  
Lung Impairment

Introduction: Asthma is a heterogenic disease, commonly divided into allergic and nonallergic asthma. It affects people of all age group and is associated with impaired lung function. Previously, it was thought that asthma is a disease of developed countries but at present, world scenario has changed and its prevalence in developing countries is rapidly increasing. Asthma shows large geographical variations in terms of prevalence, severity and mortality. Aim: To establish the prevalence of allergic and nonallergic asthma in adults of Kumaun region of Uttarakhand (India) and to evaluate whether the peripheral blood cell count is associated with the severity of lung impairment in both the subtypes (allergic and nonallergic) of asthma. Materials and Methods: This cross-sectional study was carried out jointly in Department of Physiology and Department of Respiratory Medicine, Government Medical College, Haldwani, Uttarakhand (India), during the period from October 2015 to January 2017. A total of 125 patients of both sexes age above 18 years, who attended the OPD of Respiratory Medicine and diagnosed asthma by the physician were enrolled in the study. The patients associated with tuberculosis, worm infestations, other allergic diseases and systemic diseases were excluded. History of Allergic Rhino-Conjunctivitis (ARC) was used for allergic sensitisation and to define allergic asthma. Classification of severity of airflow limitation was done according to Global Initiative for Asthma (GINA) guidelines. Absolute Leukocyte Counts was determined by using the formula: Absolute leukocyte count=Differential leukocyte count/100×Total leukocyte count. One-way ANOVA followed by Tukey HSD post-hoc analysis and Chi-square was done. Level of statistical significance was set at p-value <0.05. Results: The prevalence of allergic asthma was more in 79 patients (63.2%), in comparison to nonallergic asthma in 46 (36.8%) and it (allergic asthma) was more common in males than females (54:25). Mean age of allergic and nonallergic group asthma patients were 51.00±14.31 and 56.11±13.14 years, respectively. In allergic asthma patients, blood eosinophil count increased significantly (p=0.001) the severity of lung impairment increased and no significant changes were observed in other blood cell counts while in nonallergic asthma over-all neutrophil count was significantly high (p=0.044) with increase in severity. Nonallergic asthma also showed increasing trend of eosinophil count as the severity of airway obstruction increased but there were no significant changes in other counts. Conclusion: The prevalence of allergic asthma is high in comparison to nonallergic asthma among adults of Kumaun region of Uttarakhand, India and it is more prevalent among males in both the groups. Peripheral blood leukocyte count might be used as biological marker to differentiate allergic asthma from nonallergic asthma.

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