scholarly journals Clinical and laboratory features of infectious mononucleosis caused by the Epstein-Barr virus

2014 ◽  
Vol 95 (5) ◽  
pp. 722-725 ◽  
Author(s):  
R A Gileva ◽  
Z A Hohlova ◽  
Yu S Chechet ◽  
A S Arsenenko ◽  
L M Povolotskaya
Keyword(s):  
G Proteins ◽  
Infectious Mononucleosis ◽  
High Frequency ◽  
Epstein Barr Virus ◽  
Mononuclear Cells ◽  
Severe Form ◽  
Immunoglobulin M ◽  
Standard Laboratory ◽  
Barr Virus ◽  
Epstein Barr

Aim. To study the clinical and laboratory characteristics of patients with infectious mononucleosis caused by the Epstein-Barr virus. Methods. In infectious hospital under observation there were 108 patients aged from 11 months to 31 years with a laboratory confirmed diagnosis of «infectious mononucleosis caused by the Epstein-Barr virus». Standard laboratory tests for blood serum immunoglobulin M to the capsid protein of Epstein-Barr virus, immunoglobulin class G proteins to early Epstein-Barr virus and the index of their avidity by ELISA were carried out. Tests for other infections were conducted if it is required. Results. Patients dominated by children aged 1-7 years - 73.1%. Adult patients was 1.8% (2 patients). Only 4.6% of the patients were sent to the hospital with a diagnosis of «infectious mononucleosis» or suspected of having the disease. The typical clinical picture of infectious mononucleosis was observed in 75% of cases, atypical - in 25%. Mononuclear cells in peripheral blood were found in less than half of the patients (44.4%), in 53.1% of cases with typical and in 18.5% of cases with atypical clinic. In the remaining patients, the diagnosis of infectious mononucleosis was verified by ELISA. In 90.4% of the cases the disease was in the moderate, and in 9.6% - in the severe form. Conclusion. The high frequency of atypical course of infectious mononucleosis caused by the Epstein-Barr virus justifies the need to include it into differential diagnosis process in case of unclear infectious disease, to identify the antibodies to the Epstein-Barr virus proteins, (immunoglobulin M and G) and the index of avidity antibodies (immunoglobulin G).

scholarly journals THE VALUE OF THE IMMUNE RESPONSE IN PATIENTS WITH EBV INFECTIOUS MONONUCLEOSIS IN PREDICTING THE COURSE AND EFFICACY OF ANTIVIRAL AND IMMUNO IMMUNOCORRECTING THERAPY

2013 ◽  
Vol 18 (1) ◽  
pp. 7-14
Author(s):  
T. A. Svintsova ◽  
D. M. Sobchak ◽  
O. V. Korochkina ◽  
G. A Kravchenko ◽  
V. V Novikov
Keyword(s):  
Immune Response ◽  
Infectious Mononucleosis ◽  
Epstein Barr Virus ◽  
Mononuclear Cells ◽  
Polyclonal Antibodies ◽  
Severity Of Illness ◽  
Control Group ◽  
Differentiation Antigens ◽  
Barr Virus ◽  
Epstein Barr

The indices of immune response were studied in 68 patients with infectious mononucleosis caused by the Epstein-Barr virus (35 males, 33 females) aged 18 to 30 years. Materials and methods. The content of soluble forms of differentiation antigens (sCD95, sCD18, sCD50, sHLAI, sCD54) has been studied with enzyme immunoassay using monoclonal antibodies Mab IC0-20 and polyclonal antibodies to the antigens of the mononuclear cells of the peripheral blood. The control group included 60 healthy volunteers matched for age and sex with the main group. The aim of this study is the assessment of the content of soluble forms of differentiation antigens in patients with infectious mononucleosis caused by Epstein-Barr virus, depending on gender, age, severity of illness, comorbidities, laboratory values, the presence of viral DNA, as well as a demonstration of their value in predicting the course and outcome of the disease and the efficacy of antiviral and immunocorrecting therapy. In patients with negative results of DNA indication of EBV a significant increase in the content of soluble forms of differentiation antigens characterizing the adhesion of leukocytes (sCD18), the activity of T-lymphocytes (sCD50), the recognition of foreign antigens (sHLAI) in the blood in comparison with patients with a positive DNA indication of EBV was determined. Conclusion. According to the results of this performed work the criterion for an adequate immune response in patients with infectious mononucleosis caused by the Epstein-Barr virus was found to be the increase of the content of soluble forms of differentiation antigens (sCD95, sCD18, sCD50 sHLAI, sCD54). In patients with exanthema, tonsillar syndrome, leukocytosis, elevation of transaminases and the presence of antibodies to capsid antigen (a/VCAIgM) the content of soluble forms of differentiation antigens (sCD95, sCD18, sCD50 sHLAI, sCD54), was higher than in patients without such symptoms. In the treatment with cycloferon in patients with cyclic course of EBV infectious mononucleosis the content of sHLAI and sCD54 at 2nd-4th weeks of treatment increased by 1.5-2 times compared with the corresponding values before treatment. In patients with reactivation of the disease monotonically low indices of all studied soluble forms of differentiation antigens persisted over the 4 weeks during patients following up. In patients with infectious mononucleosis caused by Epstein-Barr virus, the dynamics of sHLAI and sCD54 after 2-4 weeks of treatment serves as secondary efficacy endpoint of antiviral, immunomodulatory therapy and the formation of the cyclic course of the disease.

Epstein-Barr Virus Infectious Mononucleosis in Children

PEDIATRICS ◽  
1985 ◽  
Vol 75 (6) ◽  
pp. 1011-1019 ◽  
Author(s):  
Ciro Valent Sumaya ◽  
Yasmin Ench
Keyword(s):  
Infectious Mononucleosis ◽  
Epstein Barr Virus ◽  
Immunoglobulin M ◽  
Antibody Responses ◽  
Adult Patients ◽  
Nuclear Antigen ◽  
Barr Virus ◽  
Age Related ◽  
Slide Test ◽  
Epstein Barr

An investigation was performed to address the need to establish the rate of positive heterophil antibody responses, oropharyngeal isolation of Epstein-Barr virus (EBV), and the evolving pattern of EBV-specific antibody responses among children with documented EBV-infectious mononucleosis. Findings showed that the rate of heterophil antibody responses appeared to increase progressively with advancing age from infancy up to 4 years, after which the rates approached values similar to that reported in young adult patients. The rapid slide test detected a heterophil antibody response as frequently as the Paul-Bunnell-Davidsohn horse cell test, except in children less than 4 years old. The decreased sensitivity found with the rapid slide test in the very young was associated with their less intense heterophil response. The younger group of children also developed a lower acute mean titer and, as a result, a decreased persistence of immunoglobulin M antibody to EBV-capsid antigen, whereas they had more frequent responses to EBV-early antigen directed to restricted component than both the older subjects and adults reported elsewhere. Antibodies to EBV-nuclear antigen, characteristically a late-onset antibody, tended to develop earlier than noted in adult patients. In contrast, the prevalence and continued excretion of EBV from oropharyngeal secretions was similar to that reported in adult patients. It is speculated that these age-related differences in host responses are associated with the ontogeny of the immunologic system.

scholarly journals Persistent Infection of Epstein-Barr Virus-Positive B Lymphocytes by Human Herpesvirus 8

1998 ◽  
Vol 72 (10) ◽  
pp. 8143-8149 ◽  
Author(s):  
Stefanie Kliche ◽  
Elisabeth Kremmer ◽  
Wolfgang Hammerschmidt ◽  
Ulrich Koszinowski ◽  
Jürgen Haas
Keyword(s):  
B Lymphocytes ◽  
Peripheral Blood ◽  
Epstein Barr Virus ◽  
Mononuclear Cells ◽  
Human Herpesvirus ◽  
Immunoglobulin M ◽  
Human Herpesvirus 8 ◽  
Herpesvirus 8 ◽  
Barr Virus ◽  
Epstein Barr

ABSTRACT In patients with Kaposi’s sarcoma (KS), human herpesvirus 8 (HHV-8) can invariably be detected in KS tumor tissue and, at a lower frequency, in prostate tissue and peripheral blood B lymphocytes. Whereas the majority of KS spindle cells are latently infected by HHV-8, linear HHV-8 genomes characteristic for lytic infection are found predominantly in the peripheral blood cells of KS patients. In this study, we show that HHV-8 can stably infect B lymphocytes in vitro in the presence of Epstein-Barr virus (EBV). We were able to generate immortalized HHV-8+/EBV+ lymphoblastoid cell lines (LCLs) derived from peripheral blood mononuclear cells (PBMC) of EBV− and EBV+ donors. In HHV-8+/EBV+ LCLs, which have the phenotype of activated B lymphocytes (CD19+, surface immunoglobulin M, CD23+, CD30+, CD80+), HHV-8 was still present after more than 25 passages (more than 9 months of culture). Latent viral transcripts and proteins were present in nonstimulated HHV-8+/EBV+ LCLs. After induction by phorbol ester and n-butyrate, HHV-8+/EBV+ LCLs expressed lytic HHV-8 transcripts and proteins. Moreover, HHV-8 could be serially passaged from HHV-8+/EBV+ LCLs to fresh PBMC.

The effectious mononucleosis assotiated with the epsteinibarr virus in children

2014 ◽  
Vol 20 (30) ◽  
pp. 64-67
Author(s):  
Ревазова ◽  
Asya Revazova ◽  
Албегова ◽  
B. Albegova ◽  
Тадеева ◽  
...  
Keyword(s):  
Infectious Mononucleosis ◽  
Clinical Picture ◽  
Epstein Barr Virus ◽  
Blood Count ◽  
Mononuclear Cells ◽  
Total Blood ◽  
Barr Virus ◽  
Epstein Barr ◽  
North Ossetia

In recent years, it is noted a widespread growth of incidence of infectious mononucleosis in children. Recorded cases do not reflect the state of the disease, as numerous inapparent mild clinical forms, cases, causing doctors questions and not registered as infectious mononucleosis. The disease is diagnosed only while a typical clinical picture and the presence of specific changes in the total blood count as atypical mononuclear cells. This disease does not have a typical clinical picture, in children, at first three years of life and adolescents. The article describes the clinical and laboratory features of infectious mononucleosis caused by the Epstein - Barr virus in children in North Ossetia Alania. The results of research will improve the quality of diagnosis of infectious mononucleosis in children in modern conditions.

Infectious Mononucleosis and Epstein Barr Virus in Childhood

PEDIATRICS ◽  
1974 ◽  
Vol 53 (3) ◽  
pp. 330-335
Author(s):  
Dov Tamir ◽  
Abraham Benderly ◽  
Joseph Levy ◽  
Edna Ben-Porath ◽  
Ami Vonsover
Keyword(s):  
Antibody Titer ◽  
Infectious Mononucleosis ◽  
Strong Correlation ◽  
Epstein Barr Virus ◽  
Blood Smear ◽  
Mononuclear Cells ◽  
Peripheral Blood Smear ◽  
Control Group ◽  
Barr Virus ◽  
Epstein Barr

The Epstein-Barr virus (EBV) antibody titer was examined in 22 children whose atypical mononuclear cells appeared in the peripheral blood smear. Paul-Bunnell and mononucleosis tests were negative in all these children, but antibodies to EBV were demonstrated in 21 of them. Only five children in a control group of 27 had antibodies to EBV. We conclude that the presence of EBV antibodies with a rising titer supports a diagnosis of infectious mononucleosis. It is of particular importance in children under 1 year of age in whom the finding of atypical mononuclear cells in the absence of positive Paul-Bunnell and mononucleosis tests shows a strong correlation with EBV antibody titer. This finding frequently occurs in the absence of typical clinical features of infectious mononucleosis.

Clinical features of infectious mononucleosis in children, depending on the etiological factor

2019 ◽  
pp. 70-73
Author(s):  
G. P. Martynova ◽  
L. A. Ikkes ◽  
Ya. A. Bogvilenе
Keyword(s):  
Infectious Mononucleosis ◽  
Epstein Barr Virus ◽  
Mononuclear Cells ◽  
Etiological Factor ◽  
Disease Etiology ◽  
Barr Virus ◽  
Immunoenzyme Method ◽  
Lymphoproliferative Syndrome ◽  
Polymerase Chain ◽  
Epstein Barr

Objective: The objective is to analyze clinical and laboratory features of infectious mononucleosis (IM) depending on disease etiology.Methods: 102 children with IM aged from 1 to 15 y.o. were examined. Polymerase chain reaction and immunoenzyme method were used to verify disease etiology.Results: In patients with Epstein–Barr virus and mixed infection, the most pronounced manifestations of lymphoproliferative syndrome and tonsillitis, high content of atypical mononuclear cells in peripheral blood are detected. Aspects of IM of cytomegaloviral etiology were notable for the prevalence of toxic syndrome along with other less pronounced clinical and laboratory manifestations of the disease.Conclusions: The detected features of the aspects of IM depending on etiological factor allow to prognosticate a variant and a character of the disease course and to early improve the therapy.

scholarly journals Evaluation of an Immunofiltration Assay That Detects Immunoglobulin M Antibodies against the ZEBRA Protein for the Diagnosis of Epstein-Barr Virus Infectious Mononucleosis in Immunocompetent Patients

2009 ◽  
Vol 16 (6) ◽  
pp. 885-888 ◽  
Author(s):  
Dayana Bravo ◽  
Beatriz Muñoz-Cobo ◽  
Elisa Costa ◽  
M. Angeles Clari ◽  
Nuria Tormo ◽  
...  
Keyword(s):  
Infectious Mononucleosis ◽  
Epstein Barr Virus ◽  
Parvovirus B19 ◽  
Human Herpesvirus ◽  
Immunoglobulin M ◽  
Nuclear Antigen ◽  
Barr Virus ◽  
Ebv Infection ◽  
Epstein Barr ◽  
Immunocompetent Patients

ABSTRACT The performance of an immunofiltration assay (IMFA) that detects immunoglobulin M (IgM) antibodies to the Epstein-Barr virus (EBV) ZEBRA (BamHI Z EBV replication activator) protein was evaluated for the diagnosis of EBV infectious mononucleosis (IM) in immunocompetent patients. The test panel consisted of 47 sera displaying an EBV-specific antibody profile compatible with an acute primary EBV infection from patients with clinical and biological features of EBV IM, 20 sera from healthy individuals either with a past EBV infection or who were EBV seronegative, 20 sera displaying an equivocal EBV antibody pattern (viral capsid antigen IgG positive [VCA IgG+], VCA IgM+, and EBV nuclear antigen-1 IgG+), and 15 sera obtained from patients with a mononucleosis-like syndrome owing to cytomegalovirus, human herpesvirus 6, or parvovirus B19. Overall, the sensitivity and the specificity of the assay were found to be 92.5%, and 97.3%, respectively. The sensitivity of the assay for the diagnosis of heterophile antibody-negative EBV IM was 86.2%. The IMFA is rapid, easy to perform, and, thus, suitable for point-of-care testing, and it may be used as a first-line test for the diagnosis of acute EBV IM in immunocompetent patients.

The Nature and Clinical Significance of Atypical Mononuclear Cells in Infectious Mononucleosis Caused by the Epstein-Barr Virus in Children

2020 ◽  
Author(s):  
Olga S Fedyanina ◽  
Anna E Filippova ◽  
Olga I Demina ◽  
Olga A Zhuliabina ◽  
Dmitry S Tikhomirov ◽  
...  
Keyword(s):  
Infectious Mononucleosis ◽  
Peripheral Blood ◽  
Epstein Barr Virus ◽  
Mononuclear Cells ◽  
Lower Percentage ◽  
Antibody Microarray ◽  
Peripheral Blood Mononuclear ◽  
Barr Virus ◽  
Ebv Infection ◽  
Epstein Barr

Abstract Atypical mononuclear cells (AM) appear in significant numbers in peripheral blood of patients with Epstein-Barr virus (EBV)-associated infectious mononucleosis (IM). We investigated the number and lineage-specific clusters of differentiation (CD) expression of atypical mononuclear cells in 110 children with IM using the anti-CD antibody microarray for panning leukocytes by their surface markers prior to morphology examination. The AM population consisted primarily of CD8+ T cells with a small fraction (0%–2% of all lymphocytes) of CD19+ B lymphocytes. AM amount in children with mononucleosis caused by primary EBV infection was significantly higher than for IM caused by EBV reactivation or other viruses and constituted 1%–53% of all peripheral blood mononuclear cells compared to 0%–11% and 0%–8%, respectively. Children failing to recover from classic IM associated with primary EBV infection within 6 months had significantly lower percentage of CD8+ AM compared to patients with normal recovery rate.

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