NFLUENCE OF THE DEGREE OF CALCIUM SUBSTITUTION BY ARGENTUM IN TRICALCIUM PHOSPHATE ON ITS BIOLOGICAL PROPERTIES IN VITRO

Results of in vitro study of argentum containing tricalcium phosphate (TCPh-Ag) are presented. It is shown that biomaterial does not possess radical forming activity. Argentum containing forms of TCPh render bacteriostatic effect upon Staphylococcus haemolyticus and Escherichia coli. Dose-dependent effect of TCPh-Ag in relation to antibacterial and cytotoxic properties is demonstrated. It is determined that TCPh with rated substitution 0.5 is characterized by moderate cytotoxicity with preservation of antibacterial properties.

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Dose-dependent effect of hydrogen peroxide on the level of transcription factor NRF2 in vitro

Problems of Biological Medical and Pharmaceutical Chemistry ◽

10.29296/25877313-2020-10-02 ◽

2020 ◽

Vol 23 (10) ◽

pp. 12-17

Author(s):

Yu.V. Abalenikhina ◽

A.V. Shchulkin ◽

P.D. Erokhina ◽

I.V. Chernykh ◽

E.N. Yakusheva

Keyword(s):

Hydrogen Peroxide ◽

Transcription Factor ◽

Dependent Effect ◽

Dose Dependent ◽

Dose Dependent Effect

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Liposomes produced by reverse phase evaporation: in vitro and in vivo efficacy of diminazene aceturate against Trypanosoma evansi

Parasitology ◽

10.1017/s0031182013002114 ◽

2014 ◽

Vol 141 (6) ◽

pp. 761-769 ◽

Cited By ~ 11

Author(s):

CAMILA BELMONTE OLIVEIRA ◽

LUCAS ALMEIDA RIGO ◽

LUCIANA DALLA ROSA ◽

LUCAS TREVISAN GRESSLER ◽

CARINE ELOISE PRESTES ZIMMERMANN ◽

...

Keyword(s):

Culture Medium ◽

Similar Efficacy ◽

Trypanosoma Evansi ◽

Diminazene Aceturate ◽

Reverse Phase ◽

Dependent Effect ◽

Dose Dependent ◽

Dose Dependent Effect

SUMMARYThis study aimed to develop and test the in vitro and in vivo effectiveness of diminazene aceturate encapsulated into liposomes (L-DMZ) on Trypanosoma evansi. To validate the in vitro tests with L-DMZ, the efficacy of a commercial formulation of diminazene aceturate (C-DMZ) was also assessed. The tests were carried out in culture medium for T. evansi, at concentrations of 0·25, 0·5, 1, 2 and 3 μg mL−1 of L-DMZ and C-DMZ. A dose-dependent effect was observed for both formulations (L-DMZ and C-DMZ), with the highest dose-dependent mortality of trypomastigotes being observed at 1 and 3 h after the onset of tests with L-DMZ. The results of in vivo tests showed the same effects in the animals treated with L-DMZ and C-DMZ in single doses of 3·5 mg kg−1 and for 5 consecutive days (3·5 mg kg−1 day−1). It was possible to conclude that T. evansi showed greater in vitro susceptibility to L-DMZ when compared with C-DMZ. In vivo tests suggest that treatment with the L-DMZ and C-DMZ showed similar efficacy in vivo. The potential of the formulation developed in this study was clearly demonstrated, as it increased the efficacy of the treatment against trypanosomosis, but more studies are needed to increase the effectiveness in vivo.

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Dexamethasone dose-dependent effect on nTreg generation in vitro in thymocytes co-cultures with JAWSII cells

Frontiers in Immunology ◽

10.3389/conf.fimmu.2013.02.00950 ◽

2013 ◽

Vol 4 ◽

Author(s):

Kowalik Anna ◽

Gugala Katarzyna ◽

Kiernozek Ewelina ◽

Kozlowska Ewa ◽

Drela Nadzieja

Keyword(s):

Dependent Effect ◽

Dose Dependent ◽

Dose Dependent Effect

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Dose-dependent effect of plants of the Lamiaceae family on the concentration of methane, fatty acids and nitrogen in the ecosystem in vitro

BIO Web of Conferences ◽

10.1051/bioconf/20224201016 ◽

2022 ◽

Vol 42 ◽

pp. 01016

Author(s):

Vitaliy Ryazanov ◽

Galimzhan Duskaev ◽

Konstantin Denisenko

Keyword(s):

Fatty Acids ◽

Volatile Fatty Acids ◽

In Vitro Study ◽

Scutellaria Baicalensis ◽

Origanum Vulgare ◽

Main Indicator ◽

Dose Dependent ◽

Endogenous Substances ◽

Dose Dependent Effect

Fermentation processes in the rumen of ruminants determine how much final metabolites and their derivatives will be formed, which are necessary for the full development of the organism, the level of productivity, and also affect the level of formation of endogenous substances, namely, greenhouse gas emissions. These criteria lead us to the search for new feed products that improve the metabolic processes of the rumen and the digestive system as a whole, so phyto-substances can serve as an alternative. The article presents the results of in vitro study of the influence of Salviae folia, Scutellaria baicalensis, Oríganum vulgáre on formation of methane, synthesis of volatile fatty acids and nitrogen, as the main indicator parameters of the enzymatic activity of the rumen of ruminants. It was found that when using phyto- substances: Salviae folia and Scutellaria baicalensis, more acetic and propionic acid was formed, Oríganum vulgare in various dosages shifted towards propionic and valeric acid. Formation of a larger amount of microbial protein (P≤0.05) with use of Salviae folia, Scutellaria baicalensis, Oríganum vulgáre in various dosages was established. Methane production decreased with use of Oríganum vulgáre.

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Dose-dependent effect of daptomycin on the artificial prolongation of prothrombin time in coagulation abnormalities: in vitro verification

BMC Pharmacology and Toxicology ◽

10.1186/s40360-017-0180-3 ◽

2017 ◽

Vol 18 (1) ◽

Author(s):

Hideki Hashimoto ◽

Makoto Saito ◽

Naoki Kanda ◽

Takehito Yamamoto ◽

Makiko Mieno ◽

...

Keyword(s):

Prothrombin Time ◽

Dependent Effect ◽

Coagulation Abnormalities ◽

Dose Dependent ◽

Dose Dependent Effect

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Effect of Precursor Deficiency Induced Ca/P Ratio on Antibacterial and Osteoblast Adhesion Properties of Ag-Incorporated Hydroxyapatite: Reducing Ag Toxicity

Materials ◽

10.3390/ma14123158 ◽

2021 ◽

Vol 14 (12) ◽

pp. 3158

Author(s):

Ozkan Gokcekaya ◽

Celaletdin Ergun ◽

Thomas J. Webster ◽

Abdurrahman Bahadir ◽

Kyosuke Ueda ◽

...

Keyword(s):

Bacterial Infections ◽

Antibacterial Properties ◽

In Vitro Study ◽

Processing Parameters ◽

Biological Properties ◽

Precipitation Method ◽

Adhesion Properties ◽

Carbonate Substitution ◽

Osteoblast Adhesion

Ag-containing hydroxyapatite (HA) can reduce risks associated with bacterial infections which may eventually require additional surgical operations to retrieve a failed implant. The biological properties of HA in such applications are strongly affected by its composition in terms of dopants as well as Ca/P stoichiometry, which can be easily controlled by altering processing parameters, such as precursor concentrations. The objective of this in vitro study was to understand the effect of variations in HA precursor solutions on antibacterial properties against Escherichia coli (E. coli) and for promoting osteoblast (bone-forming cell) adhesion on Ag incorporated HA (AgHA) which has not yet been investigated. For this, two groups of AgHAs were synthesized via a precipitation method by adjusting precursor reactants with a stoichiometric value of 1.67, being either (Ca + Ag)/P (Ca-deficient) or Ca/(P + Ag) (P-deficient), and were characterized by XRD, FTIR, and SEM-EDS. Results showed that Ag+ incorporated into the Ca2+ sites was associated with a corresponding OH− vacancy. Additional incorporation of CO32− into PO43− sites occurred specifically for the P-deficient AgHAs. While antibacterial properties increased, osteoblast adhesion decreased with increasing Ag content for the Ca-deficient AgHAs, as anticipated. In contrast, significant antibacterial properties with good osteoblast behavior were observed on the P-deficient AgHAs even with a lower Ag content, owing to carbonated HA. Thus, this showed that by synthesizing AgHA using P-deficient precursors with carbonate substitution, one can keep the antibacterial properties of Ag in HA while reducing its toxic effect on osteoblasts.

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