scholarly journals Perlakuan Rizobakteri Pada Dua Varietas Tomat (Lycopersicum esculentum Mill.) Terhadap Patogen Terbawa Benih dan Peranannya Dalam Pemacu Pertumbuhan Tanaman

2020 ◽  
Vol 4 (1) ◽  
pp. 239-248
Author(s):  
Amelia Syahdani ◽  
Siti Hafsah ◽  
Syamsuddin Syamsuddin
Keyword(s):  
Plant Growth ◽  
Rhizoctonia Solani ◽  
Seed Treatment ◽  
Sclerotium Rolfsii ◽  
Seed Viability ◽  
Test Method ◽  
Lycopersicum Esculentum ◽  
Randomized Design ◽  
Completely Randomized Design

Abstrak. Tomat merupakan salah satu produk hortikultura yang sangat banyak diminati di kalangan masyarakat karena dapat dimanfaatkan sebagai bumbu masakan, pewarna makanan, kosmetik dan obat-obatan, namun tanaman ini sering diserang oleh organisme pengganggu tanaman khususnya penyakit. Perlakuan benih secara hayati (Biological Seed Treatment) menggunakan rizobakteri merupakan salah satu inovasi yang dikembangkan untuk pengendalian penyakit dan pemacu pertumbuhan tanaman. Penelitian ini telah dilaksanakan di Laboratorium Ilmu dan Teknologi Benih Program Studi Agroteknologi Fakultas Pertanian Unsyiah. Penelitian dimulai sejak bulan Mei hingga Agustus 2017. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola non faktorial pada percobaan I terdiri dari 18 perlakuan yang diulang sebanyak 3 kali sehingga didapatkan masing-masing 54 satuan percobaan terhadap patogen Rhizoctonia solani dan 54 satuan percobaan terhadap patogen Sclerotium rolfsii sehingga didapatkan 108 total satuan percobaan. Pada perobaan II menggunakan Rancangan Acak Lengkap (RAL) pola faktorial terdiri dari 19 perlakuan rizobakteri dan 2 perlakuan varietas yang diulang sebanyak 2 kali Sehingga terdapat 38 kombinasi perlakuan yang terdiri dari 48 unit percobaan dengan 25 unit tanaman di setiap perlakuan. Hasil pengujian in vitro percobaan I dengan menggunakan metode uji kultur ganda dari 18 isolat rizobakteri terdapat lima isolat yang berpotensi sebagai agens biokontrol dengan memiliki daya hambat paling efektif yaitu HWI 4(1), HWI 5(1), HWI 5(4), HWI 8(6) dan BS3 4(5) terhadap Rhizoctonia solani namun memiliki daya hambat yang relatif rendah terhadap patogen Sclerotium rolfsii. Sedangkan pada Hasil penelitian percobaan II menunjukkan bahwa isolat yang efektif sebagai rizobakteri pemacu pertumbuhan tanaman (RPPT) terhadap proses perkecambahan benih tomat dalam meningkatkan viabilitas dan vigor benih adalah isolat SRK 5(3) dan HWI 4(5). Pada parameter pertumbuhan bibit isolat SRK 5(5) dan HWI 5(4) cenderung menunjukkan pengaruh yang efektif dibandingkan perlakukan lainnya. Varietas Servo lebih baik dibandingkan varietas Lentana terhadap viabilitas dan vigor benih. Treatment of Rizobakteri In Two Tomato Varieties (Lycopersicum esculentum Mill.) to pathogen carried seed and its role plant growth promotingAbstract. Tomato is one of the most popular horticultural products in the community because it can be used as food spices, food coloring, cosmetics and medicines, but this plant is often attacked by plant pest organisms, especially diseases. Biological Seed Treatment using rhizobacteria is one of the innovations developed for disease control and growth of plants. This research has been carried out in Agricultural Science and Technology Laboratory of Agrotechnology Majors Faculty of Agriculture Unsyiah. The study was conducted from May to August 2017. This study used a Completely Randomized Design (RAL) of non factorial pattern in experiment I consisted of 18 repeated treatments 3 times so that each of 54 experiments on pathogenic Rhizoctonia solani and 54 units of experiments Sclerotium rolfsii pathogens to obtain 108 total unit experiments. In the second experiment using Completely Randomized Design (RAL) the factorial pattern consisted of 19 rhizobacterial treatments and 2 repeated treatments of varieties 2 times. Thus there were 38 treatment combinations consisting of 48 experimental units with 25 plant units in each treatment. In vitro experimental results of experiment I using double culture test method from 18 rizobakteri isolates, there are five isolates that have potential as biocontrol agent with the most effective inhibition of HWI 4 (1), HWI 5 (1), HWI 5 (4), HWI 8 (6) and BS3 4 (5) to Rhizoctonia solani but have relatively low inhibitory resistance to Sclerotium rolfsii pathogens. While the results of the experimental study II showed that the effective isolates as plant growth rhizobacteria (RPPT) on the tomato seed germination process in increasing seed viability and vigor are isolates SRK 5 (3) and HWI 4 (5). In the growth parameters of seed isolates SRK 5 (5) and HWI 5 (4) tended to show an effective effect compared to other treatments. Servo variety is better than Lentana varieties on seed viability and vigor.

scholarly journals Perlakuan Rizobakteri Terhadap Patogen Terbawa Benih dan Peranannya Sebagai Pemacu Pertumbuhan Tanaman Terhadap Viabilitas dan Vigor Benih Pada Dua Varietas Cabai Merah (Capsicum annuum L.)

2020 ◽  
Vol 4 (1) ◽  
pp. 219-228
Author(s):  
Malikul Mulki ◽  
Halimursyadah Halimursyadah ◽  
Syamsuddin Syamsuddin
Keyword(s):  
Plant Growth ◽  
Capsicum Annuum ◽  
Seed Treatment ◽  
Phytophthora Capsici ◽  
Seed Viability ◽  
Agricultural Science ◽  
Colletotrichum Capsici ◽  
Capsicum Annuum L ◽  
Randomized Design ◽  
Completely Randomized Design

Abstrak. Rendahnya produksi tanaman cabai merah di Indonesia antara lain disebabkan oleh serangan penyakit dan tidak tersedianya benih yang bermutu tinggi atau memiliki viabilitas yang rendah. Perlakuan benih secara hayati (Biological Seed Treatment) menggunakan rizobakteri merupakan salah satu inovasi yang dikembangkan untuk pengendalian penyakit dan pemacu pertumbuhan tanaman. Penelitian ini telah dilaksanakan di Laboratorium Ilmu dan Teknologi Benih Program Studi Agroteknologi Fakultas Pertanian Unsyiah. Penelitian dimulai sejak bulan Mei hingga Juli 2017. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola non faktorial pada percobaan I terdiri dari 18 perlakuan yang diulang sebanyak 3 kali sehingga didapatkan masing-masing 54 satuan percobaan terhadap patogen Phytophthora capsici dan 54 satuan percobaan terhadap patogen Colletotrichum capsici sehingga didapatkan 108 total satuan percobaan. Pada perobaan II menggunakan Rancangan Acak Lengkap (RAL) pola faktorial terdiri dari 19 perlakuan rizobakteri dan 2 perlakuan varietas yang diulang sebanyak 2 kali Sehingga terdapat 38 kombinasi perlakuan yang terdiri dari 48 unit percobaan dengan 25 unit tanaman di setiap perlakuan. Hasil penelitian percobaan I menunjukkan bahwa isolat SRK 5(1) yang berasal dari Desa Serulee Kayu, Kecamatan Bukit, Kabupaten Bener Meuriah mampu menekan pertumbuhan patogen C. capsici dengan nilai daya hambat 82,22% dan terhadap patogen P. capsici 71,11%. Hasil percobaan II menunjukkan bahwa rizobakteri yang efektif sebagai pemacu pertumbuhan tanaman (RPPT) terhadap proses perkecambahan benih cabai merah dalam meningkatkan viabilitas dan vigor benih terhadap potensi tumbuh maksimum dan daya berkecambah adalah isolat KTK 8(5) dimana varietas PM999 lebih baik dibandingkan varietas Taro, namun isolat yang berbeda yaitu SRK 5(1), HWI 4(1) dan BS3 5(3) mampu meningkatkan indeks vigor pada varietas Taro yang lebih tinggi dibandingkan dengan varietas PM999. Sedangkan pengaruh perlakuan rizobakteri terhadap pertumbuhan bibit cabai merah belum menunjukkan peningkatan yang signifikan terhadap dua varietas yang dicobakan.Effectiveness of rizobacteria inhibition to Pathogens Carried Seed In vitro and its role as plant growth promoting to seed viability and vigor in two varieties red chili pepper (Capsicum annuum L.)Abstract. The low production of red pepper plants in Indonesia is partly caused by disease attacks and unavailability of high quality seeds or have low viability. Biological Seed Treatment using rhizobacteria is one of the innovations developed for disease control and growth of plants. This research has been carried out in Agricultural Science and Technology Laboratory of Agrotechnology Study Program Faculty of Agriculture Unsyiah. The study was conducted from May to July 2017. This study used a Completely Randomized Design (RAL) of non factorial pattern in experiment I consisted of 18 treatments repeated 3 times so that each of 54 experiments on pathogenic Phytophthora capsici and 54 units of experiments was obtained pathogen Colletotrichum capsici to obtain 108 total unit of experiment. In the second experiment using Completely Randomized Design (RAL) the factorial pattern consisted of 19 rizobacterial treatments and 2 repeated treatments of varieties 2 times. Thus there were 38 treatment combinations consisting of 48 experimental units with 25 plant units in each treatment. The result of experiment I showed that isolate SRK 5 (1) from Serulee Kayu Village, Bukit Subdistrict, Bener Meuriah Regency was able to suppress the growth of C. capsici pathogens with 82.22% inhibition and against P. capsici 71.11% . The results of experiment II showed that rizobakteri effective as plant growth enhancer (RPPT) to germination process of red chilli seedlings in increasing seed viability and vigor to maximum growth potential and germination is isolate KTK 8 (5) where the varieties of PM999 is better than Taro varieties, but the different isolates of SRK 5 (1), HWI 4 (1) and BS3 5 (3) were able to increase the vigor index on higher Taro varieties compared to the PM999 varieties. While the effect of rizobacterial treatment on the growth of red chili seedlings has not shown a significant increase in the two varieties tested.

scholarly journals Seed treatment for management of Meloidogyne javanica in soybean

2017 ◽  
Vol 38 (5) ◽  
pp. 2995 ◽  
Author(s):  
Adriely Alves de Almeida ◽  
Vinicius Hicaro Frederico Abe ◽  
Ricardo Marcelo Gonçalves ◽  
Maria Isabel Balbi-Peña ◽  
Débora Cristina Santiago
Keyword(s):  
Seed Treatment ◽  
Meloidogyne Javanica ◽  
Vitro Assay ◽  
Juvenile Mortality ◽  
Second Stage ◽  
Final Population ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Soybean Plants

The objective of this study was to evaluate the efficacy of the active ingredient abamectin, either in isolation or in combination with fungicides and insecticides formulated for the industrial seed treatment, on the population of Meloidogyne javanica, tested under greenhouse and in vitro conditions. In both tests, the combination of the following treatments was assessed: abamectin; thiamethoxam; fludioxonil + metalaxyl-M + thiabendazole. Water was used as control for the in vitro assay, whereas under greenhouse conditions, controls were inoculated and not inoculated with M. javanica. The tests were conducted in a completely randomized design with six (in vitro) and ten (greenhouse) replicates. For in vitro studies the effect on hatching, motility, and mortality of juveniles of M. javanica was evaluated. Under greenhouse conditions, the soybean ‘BRS 133’ seeds were treated, and at 15, 30, and 60 days after inoculation (DAI) with M. javanica, plant measurements were recorded. The penetration of second stage juveniles (J2) was also evaluated at 15 DAI. At 30 DAI, galls, egg masses, nematodes/g of root, and final population were evaluated. At 60 DAI, the final population of nematodes in the roots was quantified. The treatments containing abamectin were the most effective in diminishing the hatching of juveniles. All treatments had an effect on nematode motility when compared to the control, and in the treatment containing only abamectin, total juvenile mortality was observed. In greenhouse conditions, at 15 DAI, the treated soybean plants had the highest root mass and shoot length, differing statistically from the inoculated control. All treatments reduced the number of nematodes per gram of root, differing from the control. At 30 DAI, treatment efficiency was observed in reducing the final population of M. javanica, particularly the treatments using abamectin, and abamectin + thiamethoxam + fludioxonil + metalaxyl-M + thiabendazole. However, at 60 DAI, the effect of the treatments on the population of M. javanica did not persist.

scholarly journals Eksplorasi Rizobakteri Indigenous Dan Uji Antagonis Terhadap Patogen Rigidoporus microporus Dan Phellinus noxius Pada Tanaman Karet (Hevea brasiliensis) Secara In Vitro

2020 ◽  
Vol 3 (4) ◽  
pp. 43-52
Author(s):  
Murtadha Murtadha ◽  
M. Abduh Ulim ◽  
Syamsuddin Syamsuddin
Keyword(s):  
Plant Growth ◽  
Hevea Brasiliensis ◽  
Growth Promoter ◽  
Randomized Design ◽  
Pathogen Control ◽  
Completely Randomized Design ◽  
Experimental Stage ◽  
Phellinus Noxius ◽  
Banda Aceh

Abstrak.  Penelitian bertujuaan untuk mendapatkan isolat rizobakteri yang mampu berperan sebagai agens biokontrol terhadap pengendalian patogen R. microporus dan P. noxius secara in vitro serta sebagai agen rizobakteri pemacu pertumbuhan tanaman (RPPT). Penelitian dilaksanakan di Laboratorium Ilmu dan Teknologi Benih Jurusan Agroteknologi, Fakultas Pertanian, Universitas Syiah Kuala, Darussalam Banda Aceh, mulai Oktober sampai Desember 2017. Penelitian ini menggunakan Rancangan Acak Lengkap non faktorial. Faktor yang diteliti yaitu isolat rizobakteri, taraf yang dicobakan terdiri dari 15 isolat dan dua patogen antagonis R. microporus dan P. noxius, yang diulang sebanyak 3 kali sehingga terdapat 90 unit satuan percobaan. Hasil penelitian menunujukkan isolat rizobakteri mampu menekan pertumbuhan koloni cendawan patogen. Pada patogen uji R. microporus terdapat 3 rizobakteri yang paling baik yaitu isolat DLG5/3 dengan persentase penghambatan 68,33%, DLG4/1 dengan persentase penghambatan 66,66% dan DLG4/7 dengan persentase penghambatan 63,33%. Pada patogen uji P.noxius terdapat dua rizobakteri yang paling baik dalam menghambat pertumbuhan koloni cendawan patogen yaitu isolat DLG5/1 dengan persentase penghambatan 60,33%, dan DKP6/3 dengan persentase penghambatan 52,50%. Pada laju penghambatan isolat rizobakteri yang paling baik pada patogen R. microporus yaitu isolat DLG6/4 dan DKP4/1 dengan nilai rerata laju penghambatan 20,33 mm/hari. Pada patogen P. noxius menunjukan isolat rizobakteri yang paling baik yaitu isolat DLG4/1 dengan nilai laju penghambatan 12,05 mm/hari.Exploration of Indigenous Rizobacteria and Antagonistic Test against Patogen Rigidoporus microporus And Phellinus noxius In Rubber Plant (Hevea brasiliensis) In VitroAbstract. The research was conducted to obtain rhizobacteria isolates capable of acting as biocontrol agents on pathogen control of R. microporus and P. noxius in vitro and as plant growth promoter rhizobacteria (PGPR). The research was conducted at the Science and Technology of seed Laboratory, Department of Agrotechnology, Faculty of Agriculture, Syiah Kuala University, Darussalam Banda Aceh, starts from October to December 2017. The research using Completely Randomized Design non factorial. Factors researched were rhizobacteria isolates, the experimental stage consisted of 15 isolates and 2 antagonist were R. microporus dan P. noxius, and 3 time repeated until be found 90 units of treatment. The results showed that rhizobacteria isolates capable to inhibith growth of colonies pathogenic. In the pathogen of R. microporus test, there are 3 better rhizobacteria were DLG5/3 isolate with 68.33% inhibition percentage, DLG4/1 with 66.66% inhibition percentage and DLG4/7 with 63,33% inhibition percentage. In the pathogen of P. noxius there are 2 best rhizobacteria inhibiting growth, DLG5/1 isolate with 60.33% inhibition percentage, and DKP6/3 with 52,50% inhibition percentage. In the pathogen of R. microporus test, there are 2 better rhizobacteria were DLG6/4 and DKP4/1 with 20,33 mm/day inhibition percentage. In the pathogen of P. noxius test,  the best rhizobacteria were DLG4/1  with 12,05 mm/day inhibition percentage. 

scholarly journals Efektivitas Limbah Cengkih dalam Menekan Perkembangan In Vitro Sclerotium rolfsii, Jamur Penyebab Damping Off Kacang Tanah

2021 ◽  
Vol 17 (1) ◽  
pp. 36-42
Author(s):  
Handry R D Amanupunyo ◽  
Nace E Tahitu ◽  
Gratiana N C Tuhumury
Keyword(s):  
Sclerotium Rolfsii ◽  
Plant Diseases ◽  
Damping Off ◽  
Flower Stalk ◽  
Colony Diameter ◽  
Randomized Design ◽  
Leaf Powder ◽  
Peanut Production ◽  
Completely Randomized Design

Various problems have caused the decline in peanut production, among others the low resistance of peanut plants to various diseases. One of the obstacles in peanut production is the attack of plant diseases, such as the fungus Sclerotium rolfsii Sacc. The use of plant-based insecticides can be an alternative to disease control in peanuts. This study aimed to obtain the appropriate and effective dose of clove leaves and flower stalks in suppressing the in vitro growth of the fungus S. rolfsii Sacc causing damping off in peanuts, and to test the effectiveness of clove leaves and flower stalks on the formation of sklerotia of the fungus. This study used ‘tuni’ clove powder from Waai Village, Salahutu District. This experimental study employed a completely randomized design (CRD) with 8 treatments and 3 replications. The results showed that the growth of the fungus S. rolfsii Sacc. was inhibited by clove flower stalk powder at a dose of 1.5 g per100 mL of PDA, which was indicated by the number of sklerotia formed of 13.67% and propagule weight of 0.12 g, followed by a dose of clove leaf powder of 1.5 g per100 mL PDA, with amount of sklerotia formed of 27.33% and propagule weight of 0.17 g. These results were statistically shown on the parameters: colony diameter, Sklerotia formation time, number of Sklerotia, and weight of propagules. Keywords: Clove powder, dosage, in vitro, Sclerotium rolfsii Sacc.   ABSTRAK Berbagai masalah telah menyebabkan turunnya produksi kacang tanah, diantaranya masih rendahnya ketahanan tanaman kacang tanah terhadap berbagai penyakit. Salah satu kendala dalam produksi kacang tanah adalah serangan penyakit tanaman, seperti jamur Sclerotium rolfsii Sacc. Penggunaan insektisida nabati dapat menjadi alternatif pengendalian penyakit pada kacang tanah. Penelitian ini bertujuan untuk memperoleh dosis daun dan tangkai bunga cengkih yang tepat dan efektif dalam menekan pertumbuhan jamur S. rolfsii Sacc penyebab damping off pada kacang tanah secara in vitro, serta menguji efektifitas daun dan tangkai bunga cengkih terhadap pembentukan sklerotia jamur. Penelitian ini menggunakan bubuk cengkih tuni, dari Desa Waai, Kecamatan Salahutu. Penelitian eksperimental ini menggunakan Rancangan Acak Lengkap (RAL) dengan 8 perlakuan dan 3 ulangan. Hasil penelitian menunjukkan bahwa pertumbuhan jamur S. rolfsii Sacc. terhambat oleh bubuk tangkai bunga cengkih pada dosis 1,5 g per 100 mL PDA yang ditunjukan oleh jumlah sklerotia terbentuk 13,67% dan berat propagul 0,12 g dan diikuti oleh dosis bubuk daun cengkih 1,5 g per 100 mL PDA, dengan jumlah sklerotia 27,33% dan berat propagul 0,17 g. Hasil ini secara statistik ditunjukkan pada parameter: diameter koloni, waktu terbentuk sklerotia, jumlah sklerotia, dan berat propagul. Kata kunci: bubuk cengkih, dosis, in vitro, Sclerotium rolfsii Sacc.

scholarly journals Efficacy of Fungicides Against Rhizoctonia solani Inciting Rhizome Rot Diseases on Large Cardamom (Amomum subulatum Roxb.)

2020 ◽  
Vol 8 (1) ◽  
pp. 61-64
Author(s):  
Ajaya Karkee ◽  
Dhanik Lal Mandal
Keyword(s):  
Rhizoctonia Solani ◽  
Agricultural Research ◽  
Research Station ◽  
Active Ingredients ◽  
Randomized Design ◽  
Mycelia Growth ◽  
Completely Randomized Design ◽  
Amomum Subulatum ◽  
Large Cardamom

An in-vitro experiment was carried out for testing the efficacy of various fungicides by adopting poisoned bait method against Rhizoctonia solani (isolated from large cardamom) in Agricultural Research Station, Pakhribas. The experiments were conducted with Completely Randomized Design (CRD) during 2016 with four replications. Two level of concentration of the fungicides (10 ppm and 100 ppm) were used and concentration was calculated based on active ingredients (a.i.) of the pesticides. At lower concentration (10 ppm), Nativo (Tubeconazole 50% + Trifloxystrobin 25 WG) and Dhanustan (Carbendazim 50 % WP) whereas at higher concentration (100 ppm), Folicure (Tubeconazole 25.9 %) and Saaf (Carbendazim 12 % + Mancozeb 63 % WP) found effective for the inhibitions of mycelia growth of R. solani. For the control of R. solani, Nativo and Dhanustan showed greatest inhibition of mycelia growth at 10 ppm concentration as compared to other fungicides. The fungicides containing Tubeconazole and Carbendazim as active ingredients, shows highest degree of mycelia inhibitions as compared to others. The fungicides found effective for inhibition of mycelia growth of R. solani in this study should be further tested in field conditions to verify their efficacy as well as to determine their optimum doses of application. Int. J. Appl. Sci. Biotechnol. Vol 8(1): 61-64

scholarly journals Daya Hambat Minyak Serai Wangi (Andropogon nordus L.) terhadap Pertumbuhan Koloni Patogen Terbawa Benih secara In Vitro dan Pengaruhnya Terhadap Viabilitas dan Vigor Benih Terung (Solanum melongena L.)

2017 ◽  
Vol 2 (4) ◽  
Author(s):  
Aldina Cahaya ◽  
Hasanuddin Hasanuddin ◽  
Syamsuddin Syamsuddin
Keyword(s):  
Seed Treatment ◽  
Design Method ◽  
Sclerotium Rolfsii ◽  
Solanum Melongena ◽  
Inhibitory Effects ◽  
Citronella Oil ◽  
Randomized Design ◽  
Oil Concentration

Abstrak. Penelitan tentang efektifitas minyak serai wangi dalam menghambat pertumbuhan koloni patogen terbawa benih secara in vitro dan pengaruhnya terhadap viabilitas dan vigor benih terung dilaksanakan di Laboratorium Ilmu dan Teknologi Benih Program Studi Agroteknologi Fakultas Pertanian Universitas Syiah Kuala yang berlangsung pada bulan Juni sampai dengan Agustus 2017. Penelitian ini bertujuan untuk mengetahui pengaruh jenis patogen dan konsentrasi minyak serai wangi serta interaksi antara jenis patogen dengan konsentrasi minyak serai wangi terhadap daya hambat pertumbuhan koloni patogen terbawa benih secara in vitro.  Penelitian ini juga bertujuan untuk mengetahui konsentrasi minyak serai wangi yang efektif terhadap viabilitas dan vigor benih terung secara in vivo. Percobaan I menggunakan Rancangan Acak Lengkap (RAL) pola faktorial terdiri atas dua faktor, faktor pertama yang diteliti terdiri dari 6 taraf konsentrasi minyak serai wangi, faktor kedua adalah 2 taraf jenis isolat patogen dengan 3 ulangan sehingga diperoleh 36 satuan percobaan. Percobaan II menggunakan Rancangan Acak Lengkap (RAL) pola non faktorial yang terdiri dari 4 perlakuan dengan 5 ulangan sehingga diperoleh 20satuan unit percobaan. Hasil percobaan I menunjukkan bahwa Daya hambat pertumbuhan Rhizoctonia solani secara sempurna (100%) diperoleh dengan pemberian minyak serai wangi pada konsentrasi 0,08%. Sementara daya hambat 100% pada patogen Sclerotium rolfsii, diperoleh dengan pemberian minyak serai wangi pada konsentrasi 0,10%. Hasil percobaan II menunjukkan bahwa perlakuan benih dengan minyak serai wangi tidak memberikan pengaruh negatif terhadap viabilitas dan vigor benih apabila konsentrasi yang digunakan dibawah 0,02% Effectiveness of In Vitro Inhibitory Effects of citronella oil (Andropogon nordus L.) on Controlling the growth of seed borne pathogens Colonies and the effect for viability and vigor of eegplant seed (Solanum melongena L).Abstract. The research of citronella oil effectivity on controlling the growth of seed borne pathogens under in vitro conditions and the effect for viability and vigor of eggplant seed.has been carried out in the laboratorium of seed, Departement of Agroteknologi, Agriculure Faculty, Syiah Kuala University was started from June until August  2017. The purpose of this study was to find out about the effect of pathoghen with citronella oil concentration and interact between pathogen with citronella oil concentration on controlling the growth of seed borne pathogens under in vitro conditions.The second purpose of this research was to find out about effectiveness of citronella oil to viability and vigor of eggplant seed under in vivo conditions. The first research used double randomize design method concisting with  two standards,the first was using  6 concentrations of citronella oil, seconf was two kinds of pathogens and repeated 3 times to obtain36 units of experiment. The second research used single randomized design method (CRD) consisting of 4 treatments and repeated 5 times to obtain 20 units of experiment. The results of the first research showed that the solubility of Rhyzoctonia solani growth (100%) was obtained by administering citronella oil at a concentration of 0.08%. While 100% inhibitory in Sclerotium rolfsii pathogens, obtained by administration of citronella oil at a concentration of 0.10%. The results of experiment II showed that the seed treatment with citronella oil did not negatively affect the viability and vigor of the seeds when the concentration used was 0.02%

scholarly journals Pertumbuhan Tanaman Nanas pada Berbagai Konsentrasi IBA Secara In Vitro

2014 ◽  
Vol 4 (2) ◽  
pp. 287-292
Author(s):  
Zarmiyeni Zarmiyeni ◽  
Siti Munawarah Munawarah
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Butyric Acid ◽  
Market Demand ◽  
Indole Butyric Acid ◽  
The People ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
A New Technique

       The people of Hulu Sungai Utara Regency are very fond of pineapple, this can be seen from the continued increase in market demand for pineapple. Pineapple production in Hulu Sungai Utara Regency from 2010-2013 continues to increase. However, the availability of pineapple is still widely supplied from other regions. This is due to limited land and the availability of superior seeds. Therefore we need a new technique to overcome this problem by multiplying it in vitro. This study aims (i) to determine the response of pineapple plant growth to various concentrations of IBA (Indole Butyric Acid) in vitro and (ii) to obtain the best IBA (Indole Butyric Acid) concentration for pineapple plant growth in vitro. The study was conducted in the Amuntai STIPER tissue culture laboratory from March to June 2014, using a Completely Randomized Design (CRD) with 5 treatment levels, 4 replications. The factor tested was the IBA concentration, i1 = 1.5 ppm, i2 = 2 ppm, i3 = 2.5 ppm, i4 = 3 ppm, and i5 = 3.5 ppm. The results showed no known response and the best concentration of IBA on pineapple plant growth.

scholarly journals UJI DAN SELEKSI ISOLAT KONSORSIUM MIKROB FILOSFER DAN RIZOSFER TERHADAP PERKECAMBAHAN BENIH PADI

2018 ◽  
Vol 8 (2) ◽  
pp. 62-72
Author(s):  
Aris Aksarah Pas ◽  
Didy Sopandie ◽  
Trikoesoemaningtyas Trikoesoemaningtyas ◽  
Dwi Andreas Santosa
Keyword(s):  
Seed Treatment ◽  
Microbial Consortium ◽  
Dry Weight ◽  
Negative Influence ◽  
Test Method ◽  
Rice Seed ◽  
Randomized Design ◽  
Wet Weight ◽  
Completely Randomized Design ◽  
Selection Of

The application of microbes on the seeds of rice plants, microbes can enter into the rice seed tissues and growth in seed tissues, these microbes can colonize and survive and develop in the seeds. Colonization will continue when the plant grows. This study aims to select the phyllosphere and rhizosphere microbial consortium which can increase plant growth. Furthermore, choosing the best phyllosphere and rhizosphere microbial consortium to test its effectiveness in increasing rice growth. The test method was carried out by soaking the rice seeds (seed treatment) on each mixture suspension for 24 hours. Sprout power was observed after 2 days of incubation and root length, shoot length, wet weight and dry weight of seedlings were observed after 5 days. The method uses a Completely Randomized Design one treatment factor. The selection of the eight best consortium isolates was chosen based on the ranking after each parameter was given the weight multiplied by the scoring. The explored samples from various ecosystems in Sigi Regency contain the phyllosphere and rhizosphere microbial consortium. The isolates of the phyllosphere and rhizosphere microbial consortium have a positive, neutral and negative influence on rice seed germination. Can be selected for each of the eight best phyllosphere and rhizosphere microbial consortiums

scholarly journals Efektivitas Rizobakteri Isolat Tanaman Tomat (Lycopersicum esculentum Mill.) terhadap Daya Hambat Pertumbuhan Koloni Patogen Fusarium oxysporum Tanaman Terung (Solanum melongena L.) secara in vitro.

2018 ◽  
Vol 3 (4) ◽  
pp. 149-158
Author(s):  
Rivayani Ahyamaqvirah ◽  
Nanda Mayani ◽  
Nanda Mayani ◽  
Syamsuddin Syamsuddin ◽  
Syamsuddin Syamsuddin
Keyword(s):  
Fusarium Oxysporum ◽  
High Activity ◽  
Tomato Plant ◽  
Solanum Melongena ◽  
Lycopersicum Esculentum ◽  
In Vitro Method ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Banda Aceh

Abstrak.  Penelitian ini bertujuan untuk mendapatkan rizobakteri isolat tanaman tomat yang mampu berperan sebagai agen biokontrol dalam menghambat pertumbuhan koloni patogen Fusarium oxysporum tanaman terung secara in vitro. Penelitian ini dilaksanakan di Laboratorium Ilmu dan Teknologi Benih Jurusan Agroteknologi, Fakultas Pertanian, Universitas Syiah Kuala, Darussalam Banda Aceh dari bulan Mei sampai Juli 2018. Rancangan yang digunakan pada uji antagonisme rizobakteri dengan patogen adalah Rancangan Acak Lengkap (RAL) non faktorial. Parameter yang diamati adalah persentase penghambatan pertumbuhan koloni patogen dan laju penghambatan pertumbuhan koloni patogen. Hasil penelitian menunjukkan persentase daya hambat rizobakteri yang tertinggi dalam menghambat pertumbuhan koloni patogen yaitu isolat SRK 5/3 dengan nilai 61,97% dengan aktivitas tinggi dan laju penghambatan isolat rizobakteri yang menekan pertumbuhan koloni, hifa, dan spora yaitu isolat SRK 5/3 dengan nilai 11,50  mm/hari.EFFECTIVENESS OF RIZOBACTERIAL TOMATO PLANT (Lycopersicum esculentum Mill.) ISOLATES ON PATHOGENIC COLUMN GROWTH POWER OF Fusarium oxysporum EGGPLANT (Solanum melongena L.) IN VITROAbstract. This study aims to obtain rhizobacteria from tomato isolates which are able to act as biocontrol agents for inhibiting the growth of pathogenic colonies of Fusarium oxysporum in eggplant plants by in vitro method. This research was carried out at the Laboratory of Seed Science and Technology, Department of Agrotechnology, Faculty of Agriculture, Syiah Kuala University, Darussalam Banda Aceh, from May to July 2018. The test of rhizobacterial antagonism with pathogens used non-factorial Completely Randomized Design (CRD). The parameters of this research were the percentage of inhibition of the pathogenic colonies growth and the rate of inhibition of the pathogenic colonies growth. The results showed that the highest percentage of rhizobacterial inhibition for hampering the growth of pathogenic colonies, SRK 5/3 isolates with 61.97% values with high activity and the rate of inhibition of rhizobacterial isolates which suppressed the growth of colony, hyphae, and spores, is 5/3 SRK isolates with the value of 11.50 mm / day.

scholarly journals ORGANOGENESIS TANAMAN KACANG HIJAU (Vigna radiata L.) PADA BEBERAPA KONSENTRASI ZAT PENGATUR TUMBUH SITOKININ DAN GIBERELIN SECARA IN VITRO

2019 ◽  
Vol 1 (2) ◽  
pp. 51-56
Author(s):  
Sartia Hama ◽  
Lisa Widianti
Keyword(s):  
Plant Growth ◽  
Vigna Radiata ◽  
Concentration Level ◽  
Average Value ◽  
Randomized Design ◽  
Bean Plants ◽  
Number Of Leaves ◽  
Stimulate Plant Growth ◽  
Completely Randomized Design

This study aims to determine the organogenesis of mung bean plants (Vigna radiata L.) in several concentrations of cytokine and gibberellin growth regulators In Vitro. The method used was a Completely Randomized Design (CRD) with 6 treatments, 4 replications so that there were 24 experimental units. The concentration level used was P0 = no treatment, P1 = 2.5 ppm BAP + 2 ppm GA, P2 = 3.5 ppm BAP + 3 ppm GA, P3 = 4.5 ppm BAP + 4 ppm GA, P4 = 5.5 ppm BAP + 5 ppm GA, P5 = 6.5 ppm BAP + 6 ppm GA. The results showed that the growth regulator BAP and GA that given to each treatment had no significant effect on all parameters, this was due to the lack of elements of nitrogen to stimulate plant growth. The most effective treatment was found in P4 with a concentration 5.5 ppm BAP + 5 ppm GA which had the average value of germination age of 2 days after planting, then the average value of plantlets height was 10.07 cm, the average number of roots and number of leaves were as much as 2 strands, while the number of plantlet weight was 0.35.

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