scholarly journals Dose-dependent effect of betamethasone on the articular cartilage (experimental study)

2021 ◽  
Vol 27 (1) ◽  
pp. 80-86
Author(s):  
A.N. Nuriakhmetov ◽  
◽  
I.F. Akhtiamov ◽  
D.E. Tsyplakov ◽  
A.M. Abdullah ◽  
...  
Keyword(s):  
Subchondral Bone ◽  
Inflammatory Cell ◽  
Left Knee ◽  
Knee Joints ◽  
Control Group ◽  
Rabbit Knee ◽  
Frequency Of Use ◽  
The Right ◽  
Dose Dependent ◽  
Dose Dependent Effect

Introduction Glucocorticosteroid injections have been widely used in clinical practice. Betamethasone is one of the agents of this group of drugs. Its efficacy and therapeutic effect with intra-articular administration are undeniable. There are special instructions on the dosage and frequency of use of the drug but unfortunately there are cases of its wrong administration. There is also an evidence of an adverse effect on cartilage both of the drug itself and its combination with local anesthetics. Aim Evaluation of the results of different weekly intra-articular protocols of betamethasone administration on histological preparations of rabbit knee joints. Methods Histological preparations of the right knee joints of three groups of rabbits were studied: after one, three, and six administrations of betamethason per week and the control intact left knee joints. Results Histological preparations of the control group and the group with a single weekly administration of the drug did not have any changes in the structure of diarthrosis. Dystrophic and necrotic changes affecting all morphological components were observed in the joints of animals that received intra-articular injections of betamethason three times a week (compared to a single injection, the area of dystrophy and necrosis of the cartilage was greater by 10.05 ± 0.75 % (p < 0.05), of subchondral bone by 8.11 ± 0.5 % (p < 0.001), and of synovium by 6.25 ± 0.32 % (p < 0.05). The group with six injections of the drug per week had the most pronounced changes. The area of necrotic changes of the cartilage was greater by 6.39 ± 0.75 % than in the group with three injections per week (p < 0.001), of subchondral bone by 11.18 ± 0.5 % (p < 0.001), of synovium by 6.12 ± 0.32 % (p < 0.001). Discussion Inflammatory cell infiltration of joint structures was absent in all cases. It indicates an aseptic nature of tissue necrosis. Evidence has been obtained between the increase in the frequency of intra-articular injections of betamethasone and the severity of dystrophic and necrotic changes in all morphological components of the joint.

Enlargement of cerebrospinal fluid spaces in long-term benzodiazepine abusers

1987 ◽  
Vol 17 (4) ◽  
pp. 869-873 ◽  
Author(s):  
C. Schmauss ◽  
J.-C. Krieg
Keyword(s):  
Low Dose ◽  
Matched Control ◽  
Control Group ◽  
High Dose ◽  
Withdrawal Therapy ◽  
The Mean ◽  
Dose Dependent ◽  
Dose Dependent Effect ◽  
Age And Sex

SynopsisIn 17 benzodiazepine (BDZ) dependent in-patients a CT scan was performed before initiation of withdrawal therapy. The evaluation of the ventricular to brain ratio (VBR) by standardized and computerized measurements revealed significantly higher mean VBRs for both high-and low-dose BDZ-dependent patients compared to the mean VBR of an age- and sex-matched control group. In addition, the mean VBR of high-dose BDZ-dependent patients (N = 8) was significantly higher than the mean VBR of low-dose BDZ-dependent patients (N = 9). This difference could not be accounted for by the age of the patients or duration of BDZ-dependency and, therefore, suggests a dose-dependent effect of BDZs on the enlargement of internal CSF-spaces. On the other hand, higher values for the width of external CSF-spaces were found to be related to increasing age of the patients and duration of BDZ-dependency.

scholarly journals How does intraarticular dexmedetomidine injection effect articular cartilage and synovium? An animal study

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Başak Akça ◽  
Aysun Ankay Yılbaş ◽  
Filiz Üzümcügil ◽  
Berkem Büyükakkuş ◽  
Elham Bahador Zırh ◽  
...  
Keyword(s):  
Pain Relief ◽  
Knee Joint ◽  
Animal Study ◽  
Left Knee ◽  
Control Group ◽  
Sprague Dawley ◽  
Adult Rats ◽  
Arthroscopic Knee ◽  
The Right

Abstract Background Intraarticular injections are widely used to provide pain relief after arthroscopic procedures and minimize the use of opioids. Dexmedetomidine has been proven to potentiate pain relief and postpone the demand for the first analgesic drug when it is used intraarticularly following arthroscopic knee procedures. However, the effects of dexmedetomidine on articular structures have not yet been evaluated. Our aim was to determine the effects of intraarticular dexmedetomidine injection on articular structures such as cartilage and synovium. Design Animal study. Methods Twenty adult rats (Sprague-Dawley) were enrolled in the study. Following appropriate aseptic and anesthetic conditions, dexmedetomidine (100 mcg/ml) (0.25 ml) was injected into the right knee joint (the study group) and normal saline solution (0.25 ml) into the left knee joint (the control group) of the rats. Four rats were sacrificed from each group on days 1, 2, 7, 14, and 21, and knee joint samples were obtained. Histologists evaluated the articular and periarticular regions and the synovium using histological sections, and a five-point scale was used to grade the inflammatory changes in a blinded manner. Results The groups were found to be similar in terms of median congestion scores, edema and inflammation scores, subintimal fibrosis, neutrophil activation and cartilage structure at each of the time intervals. Conclusion In our placebo-controlled, in vivo trial, the intraarticular use of dexmedetomidine seemed to be safe with respect to the studied histopathological parameters. However, complementary studies investigating the histopathological effects, analgesic dosage and adverse effects of dexmedetomidine on damaged articular structure models are needed.

scholarly journals The effectiveness of different regimens of testosterone gel therapy in men with androgen deficiency

2020 ◽  
Vol 10 (1) ◽  
pp. 57-60
Author(s):  
Andrey V. Kuzmenko ◽  
Timur A. Gyaurgiev ◽  
Yulia Yu. Bakutina ◽  
Alena Yu. Zarubayco
Keyword(s):  
Behavioral Therapy ◽  
Control Group ◽  
Total Testosterone ◽  
Androgen Deficiency ◽  
Testosterone Deficiency ◽  
Duration Of Treatment ◽  
Basic Therapy ◽  
Testosterone Gel ◽  
Dose Dependent ◽  
Dose Dependent Effect

The results of androgen replacement therapy with the appointment of a transdermal gel with testosterone in patients with androgen deficiency were presented. 90 men with testosterone deficiency (12 nmol/L) and impaired erectile function were observed. The average age of patients was 58 5.2 years. Patients were divided into 3 groups. Patients of the 1st (control) group underwent basic behavioral therapy, the 2nd group received basic therapy with testosterone gel for a dose of 50 mg, the 3rd group received basic therapy with testosterone gel at a dose of 100 mg. The duration of treatment was 6 months. The use of testosterone in the form of a transdermal gel led to a significant increase in the content of total testosterone in both patients of the 2nd (50 mg) and 3rd groups (100 mg). A dose-dependent effect was noted, in patients of the 3rd group the level of testosterone was significantly higher than in patients 2nd group. An increase in testosterone was accompanied by a decrease in FSH and LH levels.

scholarly journals Radiological Assessment of Femoral Bicondylar Angle among Persons Attending a Tertiary Health Care

2019 ◽  
Vol 21 (1) ◽  
pp. 44-47
Author(s):  
Allin Pradhan ◽  
CP Lama ◽  
S Dhungel ◽  
SK Ghosh
Keyword(s):  
Health Care ◽  
Knee Joint ◽  
Left Knee ◽  
Knee Joints ◽  
Teaching Hospitals ◽  
Radiological Assessment ◽  
Left Femur ◽  
The Mean ◽  
The Difference ◽  
The Right

 Femoral bicondylar angle is the angle between an axis through the shaft of the femur and a line perpendicular to the infracondylar plane. This study aims to assess femoral bicondylar angle measured from radiograph of femur and knee joints obtained from teaching hospitals in Kathmandu Nepal. Total of two hundred AP view radiograph of knee joint were collected, out of which, 50 each were of male right and left knee joint and 50 each were of female right and left knee joint. The mean angle for the right male femur was 7.86° with the range of 5°-10° and mean angle for the right female femur was 8.82° with the range of 6°-11°. On the left side, bicondylar angle ranged in male from 6° -10° with the average of 7.46° and in female range was 6°-11° and average was 8.66°. The bicondylar angle was higher in female on both the side, the difference was statistically significant on the left side (P=0.004) and significant on the right side (P=0.001). The finding of the study showed the femoral bicondylar angles were greater in right femur than left femur in both sexes. The difference in the bicondylar angle between the right and left femur was statistically insignificant in both sexes. (male p=0.144, female p=0.541). The result from this study has shown that femoral bicondylar angles were generally greater amongst the females as compared to the males; greater in right femur than left femur in both sexes.

Behavioral analysis of anxiolytic action of phenazepam in conditions of an acute psychogenic stress (predator presentation) in Danio rerio

2021 ◽  
Vol 19 (1) ◽  
pp. 71-78
Author(s):  
Andrei A. Lebedev ◽  
Aleksandr S. Devyashin ◽  
Aleksandra A. Blazhenko ◽  
Sergei V. Kazakov ◽  
Viktor A. Lebedev ◽  
...  
Keyword(s):  
Danio Rerio ◽  
Path Length ◽  
Stress Test ◽  
Control Group ◽  
The Novel ◽  
Highly Sensitive ◽  
Psychogenic Stress ◽  
Pharmacological Substance ◽  
Dose Dependent ◽  
Dose Dependent Effect

We studied the effect of benzodiazepine anxiolytic phenazepam after the predator presentation to Danio rerio. The test of novelty was used: the fish was placed first in a beaker with a dissolved pharmacological substance (or H2O) and then into a novel tank for 6 min, where the trajectory, the path length, the number of movements to the upper part of the novel tank, the number and time of the pattern of freezing were measured. It is shown that, in response to the novelty of tank, the fish are reacted by submerging to the bottom, increasing the frizing, and reducing the number of movements to the upper half of the novel tank. After phenazepam administration, the fish were not only in the lower, but also in the upper part of the novel tank. The average path length did not change significantly in the range of doses used. The number and time of the frizing, as well as the time spent in the lower part of the novel tank, decreased more than 2 times compared to the control group of animals and showed a dose-dependent effect. The number of movements to the upper part of the novel tank for the experiment increased significantly from 1 in the control to 57 after phenazepam in a dose of 1 mg/l. At the same time, the number of movements of fish to the upper part of the novel tank significantly increased more than 2 times from 3th min of the experiment with the use of phenazepam in a dose of 1 mg/l. Predator presentation (Hypsophrys nicaraguensis) caused an increase in the number of freezing (temporary immobilization) and a decrease in the length of the trajectory of movement in the novel tank as compared with the Danio rerio control group. Phenazapam at a dose of 1 mg/l removed the effects of a predator, while exhibiting a typical effect: the number of movements to the upper part of the tank during the experiment significantly increased to 30; the time at the bottom of the tank was halved. It was concluded that the novelty stress test and the test with a predator are highly sensitive for studying anxiety-phobic reactions in Danio rerio.

Changes in peptidergic fiber density in the synovium of mice with collagenase-induced acute arthritis

2015 ◽  
Vol 93 (6) ◽  
pp. 435-441 ◽  
Author(s):  
Kohei Murakami ◽  
Hiroshi Nakagawa ◽  
Kazuhiko Nishimura ◽  
Saburo Matsuo
Keyword(s):  
Synovial Tissue ◽  
Left Knee ◽  
Nerve Fibers ◽  
Knee Joints ◽  
Poor Correlation ◽  
Fiber Density ◽  
Pgp 9.5 ◽  
Protein Gene Product ◽  
Collagenase Injection ◽  
The Right

The effect of acute osteoarthritis (OA) on peripheral nerve fibers (NFs) in synovial tissue, and their association with histological changes were investigated in collagenase-induced OA mice. Collagenase (10 U in 5 μL saline) was injected into the right knee, and the same volume of saline was injected into the left knee as the control. Mice were sacrificed 1, 2, 3, and 4 weeks after the collagenase injection. Histopathological changes in the knee joints were evaluated. The numbers of protein gene product (PGP) 9.5-, calcitonin-gene-related peptide (CGRP)-, and substance P (SP)-positive NFs in the synovial tissue were determined, and their densities in the tissue were calculated. The densities of PGP 9.5- and CGRP-positive NFs in the synovium were drastically decreased 1 week after the collagenase injection. However, by week 4, the density of PGP 9.5- and CGRP-positive NFs had recovered to 84% and 79% of their normal levels, respectively. Despite the poor correlation between the synovitis score and the density of CGRP- or SP-positive NFs in the synovium, the ossification rate of chondrophytes in chondro/osteophyte lesions correlated strongly with the density of CGRP-positive NFs (R = 0.855). These results suggest that the ossification of chondrophytes occurred in parallel with the increase in CGRP-positive fiber density in the synovium during the acute phase of collagenase-induced OA.

scholarly journals Assessment of dose-dependent effects of anxiolytics of benzodiazepine structure with diazepam as an example in Danio Rerio

2020 ◽  
Vol 18 (1) ◽  
pp. 43-49
Author(s):  
Aleksandr S. Devyashin ◽  
Aleksandra A. Blazhenko ◽  
Viktor A. Lebedev ◽  
Andrei A. Lebedev ◽  
Eugenii R. Bychkov ◽  
...  
Keyword(s):  
Danio Rerio ◽  
Stress Test ◽  
Dose Range ◽  
Control Group ◽  
The Novel ◽  
Gaba A ◽  
Small Doses ◽  
Pharmacological Substance ◽  
Dose Dependent ◽  
Dose Dependent Effect

Abstract. The effect of benzodiazepine anxiolytic diazepam in Danio rerio was investigated. Methods. A stress test on novelty situation was used: a fish was placed first in a beaker with a dissolved pharmacological substance (or water) and then into a novel tank for 6 min, where the trajectory, the path length, the number of movements to the upper part of the novel tank, the number and time of the pattern of freezing of the experiment were measured. Results. In response to the novelty of tank, the fish was shown to react by submerging to the bottom, increasing the freezing, and reducing the number of movements to the upper half of the novel tank. After diazepam exposure (administration), the fish was not only in the lower, but also in the upper part of the novel tank. A pharmacological analysis of diazepam effect in Danio rerio showed that in a certain dose range of 110 mg/l anxiolytic reduces (in comparison with the control group of fish) the number and time of freezing, increases the number of movements in the upper half of the tank and the swimming time in upper part of the tank. Diazepam causes a disinhibition of motor activity at doses of 1 and 5 mg/l, which may be explained by the effect of small doses of tranquilizers on presynaptic GABA-A autoreceptors. Diazepam 20 mg/l has a depriving effect. Conclusion. Diazepam acts in a higher dose range (110 mg/l) than phenazepam (0.11 mg/l) in Danio rerio. At the same time, diazepam is characterized by a domed dose-dependent effect, in contrast to the action of phenazepam. The prospect of using Danio rerio as an animal model in behavioral pharmacology is not inferior to studies in rodents.

scholarly journals Study of thermo-regulation as a worsening marker of experimental sepsis in an animal model

2020 ◽  
Vol 28 ◽  
Author(s):  
André Luiz Thomaz de Souza ◽  
Marcelo Eduardo Batalhão ◽  
Evelin Capellari Cárnio
Keyword(s):  
Body Temperature ◽  
Saline Solution ◽  
Control Group ◽  
Experimental Sepsis ◽  
Plasma Lactate ◽  
Model Method ◽  
Sepsis Model ◽  
Plasma Nitrate ◽  
Dose Dependent ◽  
Dose Dependent Effect

Objective: to analyze variations in body temperature and in plasma nitrate and lactate concentrations in rats submitted to the experimental sepsis model. Method: a total of 40 rats divided equally into five groups. The induction of endotoxemia was performed with intravenous administration of lipopolysaccharide, 0.5 mg/Kg, 1.5 mg/Kg, 3.0 mg/Kg, and 10 mg/Kg, respectively. The control group received 0.5 mL of saline solution. The experiment lasted six hours, with evaluations performed at 0 (baseline data), 2nd, 4th, and 6thhours. Results: The animals that received doses up to 3.0 mg/kg showed a significant increase in body temperature compared to the group with 10 mg/kg, which showed a decrease in these values. The increase in plasma nitrate and lactate concentrations in the groups with lipopolysaccharide was significantly higher than in the group that received the saline solution and was correlated with the increase in body temperature. Conclusion: the variations in body temperature observed in this study showed the dose-dependent effect of lipopolysaccharide and were correlated with the increase in the concentrations of nitrate and plasma lactate biomarkers. The implications of this study are the importance of monitoring body temperature, together with the assessment of these pathophysiological markers, which suggest worsening in the prognosis of sepsis.

Modification of Patients’ Sensitivity to Galvanic Current After the Administered Systemic Cryotherapy

2021 ◽  
Vol 63 (2) ◽  
pp. 88-92
Author(s):  
Joanna Łuczak ◽  
Joanna Klonowska ◽  
Joanna Michalik
Keyword(s):  
Cycle Ergometer ◽  
Left Knee ◽  
Wilcoxon Test ◽  
Knee Joints ◽  
Electrode Placement ◽  
Study Group ◽  
Upper Limbs ◽  
Galvanic Current ◽  
Test Position ◽  
The Right

Aim: The purpose of the study was to evaluate the sensitivity of patients to galvanic current after systemic cryotherapy. Material and Methods: Study group: 77 physiotherapists – hospital employees, aged 21-64 (AVG 29.4±9.5). The sensitivity was evaluated four times: before and immediately after the systemic cryotherapy procedure (1 session, 2 minutes, temperature -120°C), after exercising on a vertical cycle ergometer (20 minutes) and 4 hours after the procedure. The intensity of the galvanic current, which caused a slight tingling, was recorded. Two electrode placements were used: longitudinal on the upper limbs and transverse over the knee joints. Results: Statistical analysis revealed that the mean sensitivity to the galvanic current measured on both upper limbs or over the knee joints increases statistically significantly after systemic cryotherapy treatments. This effect persists even after 4 hours (Friedman’s ANOVA, p<0.001). Statistically significant increases in sensitivity to galvanic current were found compared to initial values for each pair of measurements, irrespective of electrode placement and test position (Wilcoxon test, p<0.01). The increase in sensitivity according to the measuring position concerned 68.8; 63.6; 72.7% of the participants on the left upper limb, on the right 61.0; 68.8; 74.0%, over the left knee joint 61.0; 68.8; 72.7% and over the right 58.4, 75.3 and 80.5% of the subjects in the study group. Conclusions: 1. After the administered systemic cryotherapy, sensitivity to galvanic current increases in most patients. 2. The increase in sensitivity to galvanic current depends on the time difference between treatments and is individually variable.

scholarly journals Intra-articular Adenosine, Lidocaine and Magnesium (ALM) solution decreases postoperative joint fibrosis in an experimental knee implant model

2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Jodie L. Morris ◽  
Hayley L. Letson ◽  
Peter McEwen ◽  
Erik Biros ◽  
Constantin Dlaska ◽  
...  
Keyword(s):  
Therapeutic Potential ◽  
Expression Profiles ◽  
Left Knee ◽  
Joint Capsule ◽  
Infrapatellar Fat Pad ◽  
Control Group ◽  
Sprague Dawley ◽  
Implant Surgery ◽  
Replacement Surgery ◽  
The Right

Abstract Background There is currently no drug therapy to prevent arthrofibrosis following knee surgery. We aimed to determine if the anti-ischemic and anti-inflammatory drug adenosine, lidocaine and Mg2+ (ALM), reduces surgery-related arthrofibrosis in a rat model of knee implant surgery. Methods Male Sprague-Dawley rats (n = 24) were randomly divided into ALM or saline groups. The right knee of each animal was implanted with custom titanium (femur) and polyethylene (tibia) implants, and the left knee served as a non-operated control. An intra-articular ALM or saline bolus (0.1 ml) was administered at the end of surgery, and animals monitored for 4 weeks. Fibrotic changes were assessed by macroscopic examination, histopathology, and expression of key inflammatory and fibrotic markers in the joint capsule and infrapatellar fat pad (IFP). Results Knee swelling was evident in both groups at 4 weeks. However, range of motion was 2-fold higher in the ALM-treated knees, and differences in macroscopic pathology indicated improved healing, compared to the control group. Histologically, ALM treatment also led to significantly decreased synovitis and fibrotic pathology in the joint capsule and IFP compared to saline controls. RNA and protein expression profiles of pro-fibrotic mediators (α-SMA, TGF-β1, FGF1, PDGFA) were also significantly lower in knees from ALM-treated animals. In addition, the expression of inflammatory mediators was lower in plasma (IL-1β, IL-10) and joint tissue (NFκB, IL-1β, IL-12), 4 weeks after surgery. Conclusion We show that intra-articular administration of a single ALM bolus significantly decreased fibrotic pathology and synovitis in an experimental model of knee implant surgery, by blunting inflammation and modulating essential genes of fibrosis. ALM has the therapeutic potential for translation into humans undergoing knee replacement surgery.

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