scholarly journals Cyanobacteria and Glutathione Applications Improve Productivity, Nutrient Contents, and Antioxidant Systems of Salt-Stressed Soybean Plant

2019 ◽  
Vol 76 ◽  
pp. 72-85
Author(s):  
Safinaz S. Zaki ◽  
Eman E.E. Belal ◽  
Mostafa M. Rady
Keyword(s):  
Glycine Max ◽  
Soluble Sugars ◽  
Foliar Spray ◽  
Plant Performance ◽  
Sand Culture ◽  
Antioxidant Systems ◽  
Guaiacol Peroxidase ◽  
Soybean Plant ◽  
Seed Inoculation ◽  
Antioxidant Defense Systems

Salt stress restricts plant performance by disrupting various physio-biochemical processes like photosynthesis. Plants growing in saline substrates show deficiencies in absorption of some essential elements due to the presence of excessive sodium (Na+) in the rhizosphere, which antagonizes beneficial cations and causing toxicity in metabolism. Cyanobacteria (CB; a natural biofertilizer) play a fundamental role in building-up soil fertility, thus increasing plant performance. Glutathione (GSH) is a well-known antioxidant, which contributes to increase salt tolerance in the plant. This work was conducted as a pot experiment (sand culture) in 2017 to study the combined effect of CB, applied as seed inoculation, and GSH, applied as foliar spray, on growth, pods and seed yields, the contents of antioxidants, osmoprotectants, and nutrients, and the antioxidative enzymes activities of soybean (Glycine max L., cv. Giza 111) plants grown under saline conditions. At fourth leaf stage (21 days after sowing; DAS), CB-pretreated seedlings were supplemented with NaCl (150 mM) along with Hoagland′s nutrient solution, and at the same time seedlings were sprayed with 1 mM GSH. Samples were taken at 60 DAS to assess morphological, physio-biochemical and antioxidant defense systems attributes. Results showed that the integrative application of CB and GSH under saline conditions was effective in improving significantly the growth characteristics, yield components, photosynthetic efficiency (pigments contents and chlorophyll fluorescence), membrane stability index, relative water content, contents of soluble sugars, free proline, ascorbic acid, glutathione, α-tocopherol, and protein, and activities of superoxide dismutase, catalase, and guaiacol peroxidase. The contents of macronutrients (N, P, K+, and Ca2+) were also increased significantly in Glycine max plants compared to the stressed control. In contrast, Na+ content and electrolyte leakage were significantly reduced. Our results recommend using the combined CB (as seed inoculation) and GSH (as foliar spray) application for soybean plantss to grow well under saline conditions.

scholarly journals Silicon Seed Priming Combined with Foliar Spray of Sulfur Regulates Photosynthetic and Antioxidant Systems to Confer Drought Tolerance in Maize (Zea mays L.)

Silicon ◽  
2021 ◽  
Author(s):  
Muhammad Farman ◽  
Fahim Nawaz ◽  
Sadia Majeed ◽  
Hafiz Muhammad Rashad Javeed ◽  
Muhammad Ahsan ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Sandy Loam ◽  
Foliar Spray ◽  
Seed Priming ◽  
Development Stage ◽  
Soil Drought ◽  
Antioxidant Systems ◽  
Guaiacol Peroxidase ◽  
Maize Seeds ◽  
The Individual

AbstractThe present study evaluated the effect of silicon (Si) seed priming and sulfur (S) foliar spray on drought tolerance of two contrasting maize hybrids viz. drought tolerant Hi-Corn 11 and susceptible P-1574. The maize seeds were primed with (3 mM Na2SiO3) or without Si (hydropriming) and later sown in pots filled with sandy loam soil. Drought stress (25–30% water holding capacity or WHC) was initiated at cob development stage (V5) for two weeks, whereas the well-watered plants were grown at 65–70% WHC. On appearance of drought symptoms, foliar spray of S was done using 0.5% and 1.0% (NH4)2SO4, whereas water spray was used as a control. The drought-stressed plants were grown for further two weeks at 25–30% WHC before the final harvest. The results showed a marked effect of Si seed priming and foliar S spray on biomass, physiological and enzymatic processes as well as macronutrient concentrations of maize. In comparison to control, the highest increase in leaf relative water content (25%), chlorophyll a content (56%), carotenoids (26%), photosynthetic rate (64%), stomatal conductance (56%) and intercellular CO2 concentration (48%) was observed by Si seed priming + S foliar spray (Si + S) under water deficit conditions. Also, Si + S application stimulated the activity of catalase (45%), guaiacol peroxidase (38%) and superoxide dismutase (55%), and improved NPK concentrations (40–63%) under water limitations. Our results suggest that Si seed priming + foliar spray of S is more effective than the individual application of these nutrients to enhance drought tolerance in maize.

scholarly journals Foliar fortification of Copper (Cu) in Glycine max L. for the protection against Asian Soybean Rust (Phakopsora pachyrhizi Syd. & P.Syd.)

2020 ◽  
Vol 7 (4) ◽  
Author(s):  
Edevan Bedin ◽  
Andréia Caverzan ◽  
Diógenes Cecchin Silveira ◽  
Geraldo Chavarria
Keyword(s):  
Glycine Max ◽  
Grain Yield ◽  
Lignin Content ◽  
Foliar Spray ◽  
Plant Performance ◽  
Soybean Rust ◽  
Phakopsora Pachyrhizi ◽  
Asian Soybean Rust ◽  
Glycine Max L ◽  
Rust Severity

The Asian Soybean Rust caused by the fungus Phakopsora pachyrhizi is one of the serious phytosanitary problems faced by soybean [Glycine max (L.) Merrill], which cause up to 80% yield loss. An alternative for the integrated management of the disease is the use of mineral nutrition together with phytosanitary treatments. Thus, the objective of this study is to understand the effect of foliar fortification with copper (Cu) along with phytosanitary treatments in the soybean reaction to Rust by lignin content variation in leaf tissues, and how it reflects the yield. The experimental design was a randomized block with four replicates. Four concentrations Cu (30, 60, 90, 120 g Cu ha-1) were tested in two distinct sources (cuprous oxide and copper carbonate) together with phytosanitary treatments. Evaluations were made to determine the progression of Asian Rust severity, micronutrient content in leaves and grains, as well as lignin content in leaves. The grain yield components and productivity were also evaluated. The Cu contents in the soybean leaves and grains were influenced by foliar spraying. Foliar spray with Cu retarded the disease progression, reducing the severity of Asian Rust and positively impacting grain yield. The amount of lignin present in the leaves was altered considerably with the application of the Cu associated with phytosanitary treatments. The results suggest that the leaf nutrition with copper together with phytosanitary treatments, may reduce the rust severity and improvement the plant performance. Future research with Cu application and analysis of specific enzymes, secondary metabolites and cell wall thickness may further contribute to the understanding of the role of Cu in defence against Asian Soybean Rust.

Bark in Woody Plants: Understanding the Diversity of a Multifunctional Structure

2019 ◽  
Vol 59 (3) ◽  
pp. 535-547 ◽  
Author(s):  
Julieta A Rosell
Keyword(s):  
Woody Plants ◽  
Fire Regime ◽  
Soluble Sugars ◽  
Plant Performance ◽  
Functional Ecology ◽  
Bark Thickness ◽  
Outer Bark ◽  
Plant Parts ◽  
Selective Forces ◽  
Plant Ecological

Abstract Most biological structures carry out multiple functions. Focusing on only one function to make adaptive inferences overlooks that manifold selection pressures and tradeoffs shape the characteristics of a multifunctional structure. Focusing on single functions can only lead to a partial picture of the causes underlying diversity and the evolutionary origin of the structure in question. I illustrate this discussion using bark as a study case. Bark comprises all the tissues surrounding the xylem in woody plants. Broadly, bark includes an inner and mostly living region and an outer, dead one. Of all plant structures, bark has the most complex anatomical structure and ontogenetic origin involving two (and often three) different meristems. Traditionally, the wide diversity in bark traits, mainly bark thickness, has been interpreted as the result of the selective pressures imposed by fire regime. However, recent research has shown that explanations based on fire regime cannot account for salient patterns of bark variation globally including the very strong inner bark thickness–stem diameter scaling, which is likely due to metabolic needs, and the very high intracommunity variation in total, inner, and outer bark thickness, and in inner:outer proportions. Moreover, explanations based on fire disregard that in addition to fire protection, bark carries out several other crucial functions for plants including translocation of photosynthates; storage of starch, soluble sugars, water, and other compounds; protection from herbivores, pathogens, and high temperatures; wound closure, as well as mechanical support, photosynthesis, and likely being involved in xylem embolism repair. All these functions are crucial for plant performance and are involved in synergistic (e.g., storage of water and insulation) and trade-off relationships (e.g., protection from fire vs photosynthetic activity). Focusing on only one of these functions, protection from fire has provided an incomplete picture of the selective forces shaping bark diversity and has severely hindered our incipient understanding of the functional ecology of this crucial region of woody stems. Applying a multifunctional perspective to the study of bark will allow us to address why we observe such high intracommunity variation in bark traits, why some bark trait combinations are ontogenetically impossible or penalized by selection, how bark is coordinated functionally with other plant parts, and as a result, to understand how bark contributes to the vast diversity of plant ecological strategies across the globe.

scholarly journals Action of growth regulators on flowering and pod production of soybean cultivar Davis

1981 ◽  
Vol 38 (1) ◽  
pp. 99-112
Author(s):  
Paulo R.C. Castro ◽  
Roberto S. Moraes
Keyword(s):  
Glycine Max ◽  
Gibberellic Acid ◽  
Growth Regulators ◽  
Lower Number ◽  
Soybean Cultivar ◽  
Soybean Plant ◽  
Trimethylammonium Chloride ◽  
Triiodobenzoic Acid ◽  
Soybean Plants

This research deals with the effects of growth regulators on flowering and pod formation in soybean plant (Glycine max cv. Davis). Under greenhouse conditions, soybean plants were sprayed with 2,3,5-triiodobenzoic acid (TIBA) 20 ppm, Agrostemmin (1g/10 ml/3 l) gibberellic acid (GA) 100 ppm, and (2-chloroethyl) trimethylammonium chloride (CCC) 2,000 ppm. Application of TIBA increased number of flowers. 'Davis' soybean treated with CCC and TIBA presented a tendency to produce a lower number of pods.

scholarly journals Effect of Seed Inoculation on Alfalfa Tolerance to Water Deficit Stress

2017 ◽  
Vol 45 (1) ◽  
pp. 82-88
Author(s):  
Mahnaz ZAFARI ◽  
Ali EBADI ◽  
Sodabeh JAHANBAKHSH GODEHKAHRIZ
Keyword(s):  
Water Deficit ◽  
Osmotic Potential ◽  
Mycorrhizal Fungi ◽  
Soluble Sugars ◽  
Compatible Solutes ◽  
Membrane Stability ◽  
Forage Yield ◽  
Water Deficit Stress ◽  
Cell Membrane Stability ◽  
Seed Inoculation

Water deficit is one of the most important environmental stresses that adversely affect crop growth and production and mycorrhizal fungi and symbiotic bacteria have important role in resistance to drought stress. The effect of biofertilizers on alfalfa stress tolerance was studied at the greenhouse condition. Treatments comprised three water-deficit stresses (35%, 55% and 75% of field capacity) and four seeds inoculations (Glomus mosseae, Sinorhizobium meliloti, G. mosseae + S. meliloti and non-inoculated). Water-deficit stress decrease cell membrane stability (39%), total Chl (24.05%), carotenoid (35.55%), quantum yield (50.64%) and forage yield (28.20%), while increased the proline and soluble sugars content (68.55 and 46.53% respectively) and osmotic potential (45.84%). The inoculation of seeds increased the capability of the plants in counteracting the stress, so that the production of compatible solutes was increased and the photosynthetic indices, proline, osmotic potential, membrane stability and forage yield were improved by seed inoculation. Mycorrhiza improved photosynthetic indexes and proline, but bacteria had more efficacy on membrane stability and forage yield. However, double inoculation due to the synergistic effect of mycorrhiza and Sinorhizobium, had the greatest effect than Solitary inoculation. Our results suggest that biofertilized alfalfa plants were better adapted than non- biofertilized ones to cope with water deficit.

scholarly journals Mechanisms of Response to Salt Stress in Oleander (Nerium oleander L.)

2016 ◽  
Vol 73 (2) ◽  
pp. 249
Author(s):  
Dinesh Kumar ◽  
Mohamad Al Hassan ◽  
Oscar Vicente ◽  
Veena Agrawal ◽  
Monica Boscaiu
Keyword(s):  
Salt Stress ◽  
Abiotic Stress Tolerance ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Nerium Oleander ◽  
Antioxidant Systems ◽  
Change Scenario ◽  
Antioxidant Compounds ◽  
Stress Dependent

Elucidating the mechanisms of abiotic stress tolerance in different species will help to develop more resistant plant varieties, contributing to improve agricultural production in a climate change scenario. Basic responses to salt stress, dependent on osmolyte accumulation and activation of antioxidant systems, have been studied in Nerium oleander, a xerophytic species widely used as ornamental. Salt strongly inhibited growth, but the plants survived one-month treatments with quite high NaCl concentrations, up to 800 mM, indicating the the species is relatively resistant to salt stress, in addition to drought. Levels of proline, glycine betaine and soluble sugars increased only slightly in the presence of salt; however, soluble sugar absolute contents were much higher than those of the other osmolytes, suggesting a functional role of these compounds in osmotic adjustment, and the presence of constitutive mechanisms of response to salt stress. High salinity generated oxidative stress in the plants, as shown by the increase of malondialdehyde levels. Antioxidant systems, enzymatic and non-enzymatic, are generally activated in response to salt stress; in oleander, they do not seem to include total phenolics or flavonoids, antioxidant compounds which did not accumulate significantly in salt-trated plants

scholarly journals Action of growth regulators on production of soybean cultivar Davis

1981 ◽  
Vol 38 (1) ◽  
pp. 127-138 ◽  
Author(s):  
Paulo R.C. Castro ◽  
Roberto S. Moraes
Keyword(s):  
Glycine Max ◽  
Gibberellic Acid ◽  
Growth Regulators ◽  
Seed Weight ◽  
Dry Weight ◽  
Soybean Plant ◽  
Trimethylammonium Chloride ◽  
Seed Number ◽  
Exogenous Growth ◽  
Soybean Plants

This research deals with the effects of exogenous growth regulators on production of soybean plant (Glycine max cv.. Davis) under greenhouse conditions, At the flower anthesis, 2,3,5-triiodobenzoic acid (TIBA) 20 ppm was applied. Other two applications with TiBA, with intervals of four days, were realized. Before flowering, Agrostemin (1 g/10 ml/3 1), gibberellic acid (GA) 100 ppm, and (2-chloroethyl) trimethylammonium chloride (CCC) 2,000 ppm were applied. It was observed that CCC and TIBA reduced stem dry weight. Soybean plants treated with TIBA reduced weight of pods without seeds , seed number and seed weight.

scholarly journals Methionine-induced regulation of growth, secondary metabolites and oxidative defense system in sunflower (Helianthus annuus L.) plants subjected to water deficit stress

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0259585
Author(s):  
Gull Mehak ◽  
Nudrat Aisha Akram ◽  
Muhammad Ashraf ◽  
Prashant Kaushik ◽  
Mohamed A. El-Sheikh ◽  
...  
Keyword(s):  
Drought Stress ◽  
Helianthus Annuus ◽  
Water Deficit ◽  
Soluble Sugars ◽  
Foliar Spray ◽  
Shoot Length ◽  
Stress Conditions ◽  
Growth And Yield ◽  
Water Deficit Stress ◽  
Growth Stages

Optimum water availability at different growth stages is one the major prerequisites of best growth and yield production of plants. Exogenous application of plant growth regulators considered effective for normal functioning of plants under water-deficit conditions. A study was conducted to examine the influence of exogenously applied L-methionine on sunflower (Helianthus annuus L.) plants grown under water-deficit conditions. Twenty-five-day old seedlings of four sunflower cultivars, FH331, FH572, FH652 and FH623 were exposed to control (100% F.C.) and drought stress (60% F.C.) conditions. After 30-day of drought stress, L-methionine (Met; 20 mg/L) was applied as a foliar spray to control and drought stressed plants. Water deficit stress significantly reduced shoot fresh and dry weights shoot and root lengths, and chlorophyll a content in all four cultivars. While a significant increase was observed due to water deficiency in relative membrane permeability (RMP), malondialdehyde (MDA), total soluble proteins (TSP), total soluble sugars (TSS), ascorbic acid (AsA) and activity of peroxidase (POD). Although, exogenously applied Met was effective in decreasing RMP, MDA and H2O2 contents, it increased the shoot fresh weight, shoot length, chlorophyll a, chlorophyll a/b ratio, proline contents and the activities of SOD, POD and CAT enzymes in all four cultivars under water deficit stress. No change in AsA and total phenolics was observed due to foliar-applied Met under water stress conditions. Of all sunflower cultivars, cv. FH-572 was the highest and cv. FH-652 the lowest of all four cultivars in shoot fresh and dry weights as well as shoot length under drought stress conditions. Overall, foliar applied L-methionine was effective in improving the drought stress tolerance of sunflower plants that was found to be positively associated with Met induced improved growth attributes and reduced RMP, MDA and H2O2 contents under water deficit conditions.

scholarly journals Identification of genes associated with productivity traits and salinity tolerance from activation tagged lines of rice

2021 ◽  
Author(s):  
Kota Vamsee Raja ◽  
Kalva Madhanasekhar ◽  
Vudem Dashavantha Reddy ◽  
Attipalli Ramachandra Reddy ◽  
Khareedu Venkateswara Rao
Keyword(s):  
Stress Tolerance ◽  
Salinity Stress ◽  
Soluble Sugars ◽  
Crop Productivity ◽  
Antioxidant Systems ◽  
Wild Type ◽  
Stress Symptoms ◽  
Effective Antioxidant ◽  
Use Efficiency ◽  
Tolerance To Salinity

AbstractWorld-wide crop productivity is hugely impacted by diverse eco-environmental conditions. In the present investigation, activation tagged (AT) lines of rice endowed with improved agronomic attributes have been analyzed for tolerance to salinity stress besides identification of genes associated with these attributes. Under salinity stress conditions, AT lines exhibited increased seed germination rates, improved plant growth and development at vegetative and reproductive stages as compared to wild-type (WT) plants. Furthermore, AT lines disclosed enhanced plant water content, photosynthetic efficiency, stomatal conductance, water use efficiency and maximum quantum yield when compared to WT plants, leading to improved yields and delayed onset of stress symptoms. Moreover, AT lines revealed effective antioxidant systems causing decreased accumulation of reactive oxygen species and delayed salinity stress symptoms compared to WT plants. Reduced accumulation of malondialdehyde with concomitant increases in proline and soluble sugars of AT lines further endorsing their improved stress tolerance levels. TAIL and qRT-PCR analyses of AT lines revealed Ds element integrations at different loci and respective overexpression of identified candidate genes involved in various aspects of plant development and stress tolerance. Accordingly, the AT lines plausibly serve as a rare genetic resource for fortifying stress tolerance and productivity traits of elite rice cultivars.HighlightActivation tagged lines of rice endowed with improved agronomic attributes have been analyzed for tolerance to salinity stress besides identification and expression analysis of genes associated with these attributes.

scholarly journals Sucrose-Enzyme Relationships in Immature Sugarcane Treated With Variable Molybdenum, Calcium, Iron, Boron, Lead, Trichloroacetic Acid, Beta-glycerophosphate, and Starch

1969 ◽  
Vol 49 (4) ◽  
pp. 443-461
Author(s):  
Alex G. Alexander
Keyword(s):  
Trichloroacetic Acid ◽  
Foliar Spray ◽  
Sand Culture ◽  
Acid Phosphatases ◽  
Starch Phosphorylase ◽  
High Iron ◽  
High Calcium ◽  
Calcium Iron

Variable levels of the elements molybdenum, calcium, iron, lead, and boron, as well as trichloroacetic acid, ß-glycerophosphate, and starch, were supplied to immature sugarcane grown in the greenhouse. Molybdenum, calcium, and iron were provided in factorial combination to plants in sand culture. Molybdenum, lead, and starch were applied as foliar sprays to a second group of plants grown in soil, and boron, ß-glycerophosphate, plus trichloroacetic acid were likewise applied to the foliage of plants grown in soil. The objectives of these experiments were to determine whether any of the applied materials could alter the action of specific enzymes, and, if so, whether significantly greater sucrose content would result. Leaf and meristem tissues were assayed for sugars, and for the enzymes amylase, invertase, acid phosphatases, starch phosphorylase, peroxidase, and polyphenol oxidase. Molybdenum significantly increased sucrose when applied as a foliar spray (80 p.p.m.), and as a nutrient in sand culture (1 p.p.m.). The molybdenum effect was retarded or reversed when either high calcium (9 p.p.m.) or high iron (6 p.p.m.) was supplied concurrently. Acid phosphatases and amylase were suppressed by high molybdenum, although these effects were greatly dependent upon calcium and iron supply. When applied as a foliar spray, molybdenum suppressed amylase and the phosphatase hydrolyzing glucose-1-phosphate, but not ATP-ase or ß-glycerophosphatase. Invertase was suppressed by high iron (6 p.p.m.) when molybdenum and calcium were low, but was stimulated when molybdenum was high. Lead, when applied to leaves at the rate of 50 p.p.m., caused moderate sucrose increases. Glucose-1-phosphate phosphatase was suppressed by lead in leaves and meristem, as was starch phosphorylase in the leaves. Foliar starch application failed to stimulate amylase, while ß-glycerophosphate failed to inhibit starch phosphorylase or to induce greater phosphatase activity. A number of enzyme responses were obtained which do not happen in vitro, and known in vitro effects did not always appear when specific materials were applied to living plants. Trichloroacetic acid, in particular, appeared to stimulate rather than inhibit enzyme action in vivo. This and other consequences of applying enzyme-regulating materials are discussed in detail.

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