scholarly journals Effect of resveratrol on diabetic neuropathy in wistar albino rats

2019 ◽  
Vol 9 (1) ◽  
pp. 16
Author(s):  
Smita Das ◽  
Jayanti Prava Behera ◽  
Y. Rojaramani ◽  
Rashmi Ranjan Mohanty
Keyword(s):  
Diabetic Neuropathy ◽  
Lipid Profile ◽  
Plasma Glucose ◽  
Fasting Plasma Glucose ◽  
High Fat Diet ◽  
Albino Rats ◽  
High Fat ◽  
Multiple Comparison Test ◽  
Wistar Albino Rats

Background: Type 2 diabetes mellitus (DM) is a common chronic disease with increasing prevalence worldwide. Prolonged uncontrolled hyperglycemia, dyslipidemia are major risk factor for its complication like neuropathy. Since there is no definite treatment for diabetic neuropathy, this study aims to evaluate the effect of resveratrol on diabetic neuropathy in high fat diet with low dose streptozotocin induced type-2 DM model in wistar albino rats.Methods: First type 2 diabetic rat model was established. Wistar albino rats, fed with high-fat diet (HFD) rendered diabetic with streptozotocin, were divided into 6 groups, disease control (DC) treated with vehicle, standard control (SC) which received metformin, test groups treated with 5, 10, and 20 mg/kg b.w. of resveratrol and combination of half dose of metformin and resveratrol (10 mg/kg) (TC). A group of six normal animals served as normal control (NC), another six as HFD control. Fasting plasma glucose, lipid profile were measured one week after induction of diabetes. The animals were then treated orally for 2 weeks after which the same parameters were repeated. Behavioral biomarkers for neuropathy are measured in 4 weeks and 6 weeks of treatment. The in-vivo results were analyzed by one way ANOVA followed by Tukey’s multiple comparison test for biochemical parameters and Kruskal Wallis test followed by Dun’s multiple comparison test for behavioral biomarkers.Results: Increase in fasting plasma glucose (FPG), deranged lipid profile, increased neuropathy in DC compared to NC, HFD control while a significant decrease in FBG, improved pain behavior with SC, test groups (p<0.05) as compared to the DC group.Conclusions: Resveratrol prevents diabetic neuropathy.

scholarly journals Fetal programming by high-fat diet promoted the decreased of the prostate in adult Wistar albino rats

2020 ◽  
Vol 164 ◽  
pp. 103649
Author(s):  
Pamella Campos-Silva ◽  
Angelo Fernandes ◽  
Waldemar Costa ◽  
Francisco Jose Sampaio ◽  
Bianca Gregorio
Keyword(s):  
Fetal Programming ◽  
High Fat Diet ◽  
Albino Rats ◽  
High Fat ◽  
Wistar Albino Rats

scholarly journals EFFECT OF SILYMARIN ON INTESTINAL ALKALINE PHOSPHATASE LEVEL IN A RAT MODEL OF TYPE 2 DIABETES MELLITUS: STREPTOZOTOCIN AND HIGH-FAT DIET TREATED WISTAR RATS

2018 ◽  
Vol 11 (11) ◽  
pp. 304
Author(s):  
Lalitha V ◽  
Sivakumar T
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Alkaline Phosphatase ◽  
Lipid Profile ◽  
High Fat Diet ◽  
Wistar Rats ◽  
Diabetic Rats ◽  
High Fat ◽  
Intestinal Alkaline Phosphatase

Objective: This research elucidated the role of silymarin on intestinal alkaline phosphatase (IAP) level in type 2 diabetic rats.Methods: The type 2 diabetes mellitus was induced by a high-fat diet (HFD - 58% calories fat) for 2 weeks, and rats were intraperitoneally injected with streptozotocin (STZ) 35 mg/kg. Wistar rats were divided into four groups. Group I served as a non-diabetic (normal), Group II served as diabetic, Group III diabetic animals treated glibenclamide 600 μg/kg for 14 days, and Group IV diabetic animal treated with glibenclamide and silymarin 50 mg/kg/twice/d for 14 days. At the end of the study, blood glucose, lipid profile, and IAP level were measured.Results: A significant decrease in IAP, elevated levels of blood glucose, and lipid profile was seen in diabetic rats when compared with normal. The silymarin treatment showed a significant increase in IAP level, a significant reduction in glucose and lipid profile than diabetic rats.Conclusion: The present study concludes that silymarin treatment enhances the IAP levels which protect against hyperglycemia, hyperlipidemia, and vascular complications in diabetic rats.

Loss of ARC worsens high fat diet-induced hyperglycemia in mice

2021 ◽  
Author(s):  
Andrew T Templin ◽  
Christine Schmidt ◽  
Meghan F Hogan ◽  
Nathalie Esser ◽  
Richard N Kitsis ◽  
...  
Keyword(s):  
Cell Death ◽  
Body Weight ◽  
Plasma Glucose ◽  
Fasting Plasma Glucose ◽  
High Fat Diet ◽  
Metabolic Adaptation ◽  
High Fat ◽  
Β Cell ◽  
Fasting Plasma

Apoptosis repressor with caspase recruitment domain (ARC) is an endogenous inhibitor of cell death signaling that is expressed in insulin-producing β-cells. ARC has been shown to reduce β-cell death in response to diabetogenic stimuli in vitro, but its role in maintaining glucose homeostasis in vivo has not been fully established. Here we examined whether loss of ARC in FVB background mice exacerbates high fat diet (HFD)-induced hyperglycemia in vivo over 24 weeks. Prior to commencing 24-week HFD, ARC-/- mice had lower body weight than wild type (WT) mice. This body weight difference was maintained until the end of the study and was associated with decreased epididymal and inguinal adipose tissue mass in ARC-/- mice. Non-fasting plasma glucose was not different between ARC-/- and WT mice prior to HFD feeding, and ARC-/- mice displayed a greater increase in plasma glucose over the first 4 weeks of HFD. Plasma glucose remained elevated in ARC-/- mice after 16 weeks of HFD feeding, at which time it had returned to baseline in WT mice. Following 24 weeks of HFD, non-fasting plasma glucose in ARC-/- mice returned to baseline and was not different from WT mice. At this final time point, no differences in plasma glucose or insulin were observed under fasted conditions or following IV glucose administration between genotypes. However, HFD-fed ARC-/- mice exhibited significantly decreased β-cell area compared to WT mice. Thus, ARC deficiency delays, but does not prevent, metabolic adaptation to HFD feeding in mice, worsening transient HFD-induced hyperglycemia.

scholarly journals Relation between Serum Leptin, Lipid Profiles and other biomarkers levels in patients with type 2 diabetic nephropathy

2010 ◽  
Vol 7 (1) ◽  
pp. 678-686
Author(s):  
Baghdad Science Journal
Keyword(s):  
Diabetic Nephropathy ◽  
Serum Creatinine ◽  
Lipid Profile ◽  
Plasma Glucose ◽  
Fasting Plasma Glucose ◽  
Control Group ◽  
Diabetic Patients ◽  
Serum Leptin ◽  
Type 2 Diabetic

Background: In advanced diabetes mellitus, serum levels of the most hormones are altered due to several interplaying mechanisms. Objective: To assess the relation of serum leptin and lipid profile in type 2 diabetic nephropathy. Patients and Method: Serum leptin levels and its relation to lipid profile were estimated in 62 patients with type 2 diabetic nephropathy attending the National Diabetes Center in Al- Mustansiriya University, and (26) healthy individuals considered as control group. The diabetic patients were classified into three groups, (24) pathients with normoalbuminuria (21) patients with microalbuminuria and (17) patients with macroalbuminuria. Fasting plasma glucose, serum creatinine, Hb A1c %, lipid profile (Total cholesterol, LDL- Cholesterol, HDL- Cholesterol and Triglyceride) and urinary albumin, were measured to establish the possibility of using these biomarkers as a supplementary to serum leptin to be a diagnostic test for type 2 diabetic nephropathy. Results: Serum leptin levels showed a significant elevation in microalbuminuria (20.08± 4.50 ng/ml) and macroalbuminuria groups (22.35± 6.89 ng/ml) as compared to nondiabetic normal control group (10.64 ± 3.17 ng/ml). There was no significant differences observed in serum leptin levels between the normoalbuminuria group (13.96 ± 5.73 ng/ml) and healthy controls, but a significant positive differences were noticed in the levels of fasting plasma glucose, serum creatinine, Hb A1c% and lipid profile in the three patient groups in comparison with the control group. While no significant correlation was observed between these biomarkers levels and serum leptin values. Conclusion: It might be concluded that serum leptin levels were elevated in type 2 diabetic patients with microalbuminuria and macroalbuminuria, suggesting that renal leptin degradation is impaired in early stage of kidney damage and this impairment increase with the progression of this disease. Leptin hormone may consider according to these results as a risk factor for progression of kidney disease in diabetic patients.

The effect of high-fat diet-induced obesity on cardiovascular toxicity in wistar albino rats

2010 ◽  
Vol 30 (9) ◽  
pp. 1313-1321 ◽  
Author(s):  
Uma Bhandari ◽  
Vinay Kumar ◽  
Naresh Khanna ◽  
Bibhu Prasad Panda
Keyword(s):  
Dietary Fat ◽  
High Fat Diet ◽  
Lipid Peroxides ◽  
Apolipoprotein A1 ◽  
Albino Rats ◽  
High Fat ◽  
Diet Induced Obesity ◽  
Healthy Control ◽  
Wistar Albino Rats

The consumption of a high-fat diet (HFD) is considered a risk factor for obesity development. Nonetheless, a causal role of dietary fat has never been documented, because of inadequate animal models. In our study, one group of rats was fed with standard rat diet, while other group of rats fed with high-fat diet for 4 weeks. After 4 weeks of feeding, the hemodynamic parameters in the rats fed with HFD were significantly increased as compared with control rats. Rats fed with HFD had elevated levels of serum lipids, insulin, leptin, glucose and apolipoprotein B. Lipid peroxides and caspase-3 levels were increased while serum apolipoprotein A1 and antioxidant enzymes levels in heart tissues were decreased in HFD-induced obesity in rats as compared to normal healthy control rats fed on standard rat pellet diet. This model of diet-induced obesity will be a useful tool for studying the mechanisms by which dietary fat induces the obesity in humans.

scholarly journals Synergistic Effect of Coconut Water and Garlic Tincture on Lipids and Oxidative Profile of Albino Rats Treated with High Fat Diet and Alcohol

2021 ◽  
pp. 1-13
Author(s):  
Holy Brown ◽  
Davies G. Tamuno-Emine ◽  
Omuruka Sweeten
Keyword(s):  
Oxidative Stress ◽  
Lipid Profile ◽  
Study Design ◽  
High Fat Diet ◽  
Colorimetric Method ◽  
Alcohol Treatment ◽  
Coconut Water ◽  
Control Group ◽  
Albino Rats ◽  
High Fat

Aim: This study evaluated the combined effect of coconut water and garlic tincture on lipid and antioxidants profile of albino rats fed with high fat diet and alcohol. Study Design: This study is a non-randomized experimental study design. Place and Duration of Study: Rivers State University Chemical Pathology Laboratory, Department of Medical Laboratory Science, the study was done between 20th November, 2018-30th June 2019. Methodology: A total number of 45 Wister albino rats were used with the weight ranged from 120-200 grams. The animals were grouped into two major groups, the control Group A and the test Group B. Blood samples were collected via cardiac puncture into heparinized bottle for standard laboratory investigation of lipid profile, and Superoxide dismutase (SOD), Total antioxidant capacity (TAC) and Malondialdehide (MDA). Plasma SOD and MDA were determined using ELISA methods, TAC was determined using FRAP Colorimetric method while lipid profile were determined using enzymatic method. Results: The results revealed that, alcohol induced oxidative stress group exhibited significant differences in MDA levels amongst the groups, and no significance differences in SOD and TAC levels as compared with negative control groups. There were significant differences in the Total Cholesterol and low density lipoprotein levels, amongst the groups. However, these changes appear to improve with coconut water and garlic tincture treatment. Treatment with coconut water alone following 30% alcohol treatment, showed a significant decrease in MDA level, no significant increase in SOD and TAC. Similar observation was recorded for the garlic tincture treatment alone. Treatment with low dose of combined coconut water with garlic tincture following 30% alcohol treatment, shows significant decrease in MDA level, significant increase in SOD, no significant increase in TAC. Treatment with low and higher doses of combined coconut water with garlic tincture following HFD treatment showed similar results, no significant decrease in TG levels, a significant decrease in MDA, TC and LDL levels, a significant increase in TAC and HDL levels and no significant increase in SOD. However, no difference was observed at higher dose. Histological findings revealed changes in hepatocellular architecture, such as inflammatory cell aggregates, dilation of sinosidal space, fatty droplet after treatment with alcohol and high fat diet. However, upon garlic tincture and coconut water treatment, there was amelioration of these abnormalities. Conclusion: The mixture of coconut water and garlic tincture seem to exerted an antioxidant and antiatherogenic effect on alcohol-induced oxidative stress and HFD-induced dyslipidaemia in rats. 

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