scholarly journals Synergistic Effect of Coconut Water and Garlic Tincture on Lipids and Oxidative Profile of Albino Rats Treated with High Fat Diet and Alcohol

2021 ◽  
pp. 1-13
Author(s):  
Holy Brown ◽  
Davies G. Tamuno-Emine ◽  
Omuruka Sweeten
Keyword(s):  
Oxidative Stress ◽  
Lipid Profile ◽  
Study Design ◽  
High Fat Diet ◽  
Colorimetric Method ◽  
Alcohol Treatment ◽  
Coconut Water ◽  
Control Group ◽  
Albino Rats ◽  
High Fat

Aim: This study evaluated the combined effect of coconut water and garlic tincture on lipid and antioxidants profile of albino rats fed with high fat diet and alcohol. Study Design: This study is a non-randomized experimental study design. Place and Duration of Study: Rivers State University Chemical Pathology Laboratory, Department of Medical Laboratory Science, the study was done between 20th November, 2018-30th June 2019. Methodology: A total number of 45 Wister albino rats were used with the weight ranged from 120-200 grams. The animals were grouped into two major groups, the control Group A and the test Group B. Blood samples were collected via cardiac puncture into heparinized bottle for standard laboratory investigation of lipid profile, and Superoxide dismutase (SOD), Total antioxidant capacity (TAC) and Malondialdehide (MDA). Plasma SOD and MDA were determined using ELISA methods, TAC was determined using FRAP Colorimetric method while lipid profile were determined using enzymatic method. Results: The results revealed that, alcohol induced oxidative stress group exhibited significant differences in MDA levels amongst the groups, and no significance differences in SOD and TAC levels as compared with negative control groups. There were significant differences in the Total Cholesterol and low density lipoprotein levels, amongst the groups. However, these changes appear to improve with coconut water and garlic tincture treatment. Treatment with coconut water alone following 30% alcohol treatment, showed a significant decrease in MDA level, no significant increase in SOD and TAC. Similar observation was recorded for the garlic tincture treatment alone. Treatment with low dose of combined coconut water with garlic tincture following 30% alcohol treatment, shows significant decrease in MDA level, significant increase in SOD, no significant increase in TAC. Treatment with low and higher doses of combined coconut water with garlic tincture following HFD treatment showed similar results, no significant decrease in TG levels, a significant decrease in MDA, TC and LDL levels, a significant increase in TAC and HDL levels and no significant increase in SOD. However, no difference was observed at higher dose. Histological findings revealed changes in hepatocellular architecture, such as inflammatory cell aggregates, dilation of sinosidal space, fatty droplet after treatment with alcohol and high fat diet. However, upon garlic tincture and coconut water treatment, there was amelioration of these abnormalities. Conclusion: The mixture of coconut water and garlic tincture seem to exerted an antioxidant and antiatherogenic effect on alcohol-induced oxidative stress and HFD-induced dyslipidaemia in rats. 

scholarly journals Cinnamomum burmannii (Nees & T. Nees) Blume and Eleutherine palmifolia (L.) Merr. extract combination ameliorate lipid profile and heart oxidative stress in hyperlipidemic mice

2020 ◽  
Vol 13 (7) ◽  
pp. 1404-1409
Author(s):  
Retno Susilowati ◽  
Abdul Malik Setiawan
Keyword(s):  
Oxidative Stress ◽  
Lipid Profile ◽  
High Fat Diet ◽  
Cholesterol Oxidase ◽  
Colorimetric Method ◽  
Thiobarbituric Acid ◽  
Stress Disorders ◽  
Control Group ◽  
Cinnamomum Burmannii ◽  
Range Test

Background and Aim: Hyperlipidemia is an important risk factor for cardiovascular disease. The use of statins has adverse side effects that result in oxidative stress disorders. The objective of this study was to investigate the antihyperlipidemic effect of a combination of Cinnamomum burmannii and Eleutherine palmifolia extract in high-fat diet (HFD)-induced hyperlipidemia mice. Materials and Methods: Mice were divided into eight groups (n=4): Control group or healthy mice (normal), HFD-induced hyperlipidemic mice without any treatment (CE0), HFD-induced hyperlipidemic mice treated with 3.6 mg/kg body weight (BW) atorvastatin (atorvastatin), and HFD-induced hyperlipidemic mice treated with a combination of C. burmannii and E. palmifolia in the following ratios: 300:0 (C300), 225:75 (C225), 150:150 (CE150), 75:225 (E225), and 0:300 (E300). Mice were fed a HFD for 4 months to induce hyperlipidemia. Total cholesterol, cholesterol oxidase-peroxidase aminophenazone (CHOD-PAP), triglyceride-glycerine, and fat serum were analyzed with colorimetric method. The measurement of superoxide dismutase was done with the xanthine oxidase method and malondialdehyde measurement was done with the thiobarbituric acid method. Results: Results showed an increase in antihyperlipidemic characteristics as the concentration of E. palmifolia extract (p<0.05) increased. Duncan's multiple range test also showed an increase in anti-stress oxidation as the concentration of C. burmannii extract (p<0.05) increased. Conclusion: The E225 group showed the most potential as a safe, antihyperlipidemic agent characterized by improvement in lipid profile and antioxidant balance.

scholarly journals Cinnamomum burmannii (Nees & T. Nees) Blume and Eleutherine palmifolia (L.) Merr. extract combination ameliorate lipid profile and heart oxidative stress in hyperlipidemic mice

2020 ◽  
Vol 13 (7) ◽  
pp. 1404-1409
Author(s):  
Retno Susilowati ◽  
Abdul Malik Setiawan
Keyword(s):  
Oxidative Stress ◽  
Lipid Profile ◽  
High Fat Diet ◽  
Cholesterol Oxidase ◽  
Colorimetric Method ◽  
Thiobarbituric Acid ◽  
Stress Disorders ◽  
Control Group ◽  
Cinnamomum Burmannii ◽  
Range Test

Background and Aim: Hyperlipidemia is an important risk factor for cardiovascular disease. The use of statins has adverse side effects that result in oxidative stress disorders. The objective of this study was to investigate the antihyperlipidemic effect of a combination of Cinnamomum burmannii and Eleutherine palmifolia extract in high-fat diet (HFD)-induced hyperlipidemia mice. Materials and Methods: Mice were divided into eight groups (n=4): Control group or healthy mice (normal), HFD-induced hyperlipidemic mice without any treatment (CE0), HFD-induced hyperlipidemic mice treated with 3.6 mg/kg body weight (BW) atorvastatin (atorvastatin), and HFD-induced hyperlipidemic mice treated with a combination of C. burmannii and E. palmifolia in the following ratios: 300:0 (C300), 225:75 (C225), 150:150 (CE150), 75:225 (E225), and 0:300 (E300). Mice were fed a HFD for 4 months to induce hyperlipidemia. Total cholesterol, cholesterol oxidase-peroxidase aminophenazone (CHOD-PAP), triglyceride-glycerine, and fat serum were analyzed with colorimetric method. The measurement of superoxide dismutase was done with the xanthine oxidase method and malondialdehyde measurement was done with the thiobarbituric acid method. Results: Results showed an increase in antihyperlipidemic characteristics as the concentration of E. palmifolia extract (p<0.05) increased. Duncan's multiple range test also showed an increase in anti-stress oxidation as the concentration of C. burmannii extract (p<0.05) increased. Conclusion: The E225 group showed the most potential as a safe, antihyperlipidemic agent characterized by improvement in lipid profile and antioxidant balance.

Swimming training combined with chitosan supplementation reduces the development of obesity and oxidative stress in high fat diet fed mice

2021 ◽  
pp. 1-31
Author(s):  
Zahra Zalaqi ◽  
Farshad Ghazalian ◽  
Mohammad Javad Khodayar ◽  
Atefeh Raesi Vanani ◽  
Layasadat Khorsandi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Profile ◽  
High Fat Diet ◽  
Dietary Intervention ◽  
The Body ◽  
Control Group ◽  
Standard Diet ◽  
High Fat ◽  
Swimming Training ◽  
Fat Diets

Abstract Obesity is often introduced as one of the metabolic disorders caused by imbalance between energy consumption and metabolisable energy intake. Experts in the field considered obesity as one of the robust risk factors for the life-style associated diseases. The present research examined interventional effects of marine chitosan (CS), swimming training (ST) and combination of CS and ST (CS+ST) in the mice fed with high fat diets (HFD). In this study, sample size was considered more than 3 in groups. Forty mice were randomly divided into 5 groups (n = 8 per group) including control group (received the standard diet), HFD group (received high fat food with 20% fat), HFD+CS group (treated with high fat food with 5% CS), HFD+ST group (treated with high fat diet and ST) and HFD+CS+ST group (treated with high fat food with 5% CS & ST). After 8 weeks the blood glucose, oxidative stress (OS), and lipid profile were measured. Results showed more efficiency of CS+ST in the control of body weight with the increased concentration of HDL-C, OS inhibition via enhancing the body antioxidant capacity in comparison to the ST or CS alone in obese mice. Moreover, lipid profile was improved in CS + ST-treated mice compared with HFD-treated mice, and OS inhibition correlated to the greater activities of the antioxidant enzyme, enhance the lipid oxidation, cholesterol and fatty acid homeostasis. The results suggested that a dietary intervention with a combined ST and CS can be a feasible supplementary for human prevention of obesity.

scholarly journals High-fat diet-induced obesity and impairment of brain neurotransmitter pool

2020 ◽  
Vol 11 (1) ◽  
pp. 147-160
Author(s):  
Ranyah Shaker M. Labban ◽  
Hanan Alfawaz ◽  
Ahmed T. Almnaizel ◽  
Wail M. Hassan ◽  
Ramesa Shafi Bhat ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Brain Tissue ◽  
High Fat Diet ◽  
Density Lipoprotein ◽  
Obese Group ◽  
Control Group ◽  
High Fat ◽  
Brain Neurotransmitter ◽  
The Brain ◽  
Lean Group

AbstractObesity and the brain are linked since the brain can control the weight of the body through its neurotransmitters. The aim of the present study was to investigate the effect of high-fat diet (HFD)-induced obesity on brain functioning through the measurement of brain glutamate, dopamine, and serotonin metabolic pools. In the present study, two groups of rats served as subjects. Group 1 was fed a normal diet and named as the lean group. Group 2 was fed an HFD for 4 weeks and named as the obese group. Markers of oxidative stress (malondialdehyde, glutathione, glutathione-s-transferase, and vitamin C), inflammatory cytokines (interleukin [IL]-6 and IL-12), and leptin along with a lipid profile (cholesterol, triglycerides, high-density lipoprotein, and low-density lipoprotein levels) were measured in the serum. Neurotransmitters dopamine, serotonin, and glutamate were measured in brain tissue. Fecal samples were collected for observing changes in gut flora. In brain tissue, significantly high levels of dopamine and glutamate as well as significantly low levels of serotonin were found in the obese group compared to those in the lean group (P > 0.001) and were discussed in relation to the biochemical profile in the serum. It was also noted that the HFD affected bacterial gut composition in comparison to the control group with gram-positive cocci dominance in the control group compared to obese. The results of the present study confirm that obesity is linked to inflammation, oxidative stress, dyslipidemic processes, and altered brain neurotransmitter levels that can cause obesity-related neuropsychiatric complications.

scholarly journals Effects of a Mixed Limosilactobacillus fermentum Formulation with Claimed Probiotic Properties on Cardiometabolic Variables, Biomarkers of Inflammation and Oxidative Stress in Male Rats Fed a High-Fat Diet

Foods ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2202
Author(s):  
Micaelle Oliveira de Luna Freire ◽  
Luciana Caroline Paulino do Nascimento ◽  
Kataryne Árabe Rimá de Oliveira ◽  
Alisson Macário de Oliveira ◽  
Thiago Henrique Napoleão ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
High Fat Diet ◽  
Control Diet ◽  
Male Rats ◽  
Control Group ◽  
Low Grade ◽  
Probiotic Properties ◽  
High Fat ◽  
Low Grade Inflammation ◽  
And Oxidative Stress

High-fat diet (HFD) consumption has been linked to dyslipidemia, low-grade inflammation and oxidative stress. This study investigated the effects of a mixed formulation with Limosilactobacillusfermentum 139, L. fermentum 263 and L. fermentum 296 on cardiometabolic parameters, fecal short-chain fatty acid (SCFA) contents and biomarkers of inflammation and oxidative stress in colon and heart tissues of male rats fed an HFD. Male Wistar rats were grouped into control diet (CTL, n = 6), HFD (n = 6) and HFD with L. fermentum formulation (HFD-Lf, n = 6) groups. The L.fermentum formulation (1 × 109 CFU/mL of each strain) was administered twice a day for 4 weeks. After a 4-week follow-up, biochemical parameters, fecal SCFA, cytokines and oxidative stress variables were evaluated. HFD consumption caused hyperlipidemia, hyperglycemia, low-grade inflammation, reduced fecal acetate and propionate contents and increased biomarkers of oxidative stress in colon and heart tissues when compared to the CTL group. Rats receiving the L. fermentum formulation had reduced hyperlipidemia and hyperglycemia, but similar SCFA contents in comparison with the HFD group (p < 0.05). Rats receiving the L. fermentum formulation had increased antioxidant capacity throughout the colon and heart tissues when compared with the control group. Administration of a mixed L. fermentum formulation prevented hyperlipidemia, inflammation and oxidative stress in colon and heart tissues induced by HFD consumption.

scholarly journals Emblica officinalis (Amla) Ameliorates High-Fat Diet Induced Alteration of Cardiovascular Pathophysiology

2019 ◽  
Vol 17 (1) ◽  
pp. 52-63 ◽  
Author(s):  
Bheemshetty S. Patil ◽  
Pallavi S. Kanthe ◽  
Chandramouli R. Reddy ◽  
Kusal K. Das
Keyword(s):  
Oxidative Stress ◽  
High Fat Diet ◽  
Ethanolic Extract ◽  
Albino Rats ◽  
Histopathological Study ◽  
Histopathological Analysis ◽  
Phytochemical Analysis ◽  
Emblica Officinalis ◽  
High Fat ◽  
Elastic Artery

Background: Dietary high fat possibly causes oxidative stress. Also, it alters the pathophysiology of metabolically active myocardial tissues and vascular architecture. Emblica officinalis contains a potential antioxidant that counteracts oxidative stress and possibly maintains vascular integrity. Objective: To assess the effect of ethanolic extract of Emblica officinalis (EEO) on High Fat Diet (HFD) induced changes in vascular chemistry and histopathology of the cardiovascular system in male albino rats. Materials and Methods: Ethanolic extract of Emblica Officinalis (EEO) was prepared and phytochemical analysis was done. Rats were divided into four groups, having six rats in each group as follows: group 1- Control (20% fat); group 2 (20% fat+ EEO 100 mg/kg/b w); group 3 (30% fat) and group 4 (30% fat + EEO 100 mg/kg/b w). Dietary and EEO supplementation was continued for 21 days. Gravimetric and oxidative stress markers like MDA, NO, antioxidants like Vitamin C and E, and molecular marker (NOS3) were evaluated. Histopathological analysis was done on the myocardium and elastic artery along with measurement of coronary arterial wall thickness and lumen diameter. One way ANOVA was done for analysis of data. Results: High fat diet showed a significant increase in MDA, decrease of NO with unaltered NOS3 protein in rats fed with high fat diet, which indicate possible alteration of vascular pathophysiology. Supplementation of EEO showed an ameliorating effect on high fat diet induced oxidative stress. These results were further corroborated with findings of a histopathological study on the myocardium, elastic artery and coronary arterial architecture. Conclusion: Ethanolic extract of Emblica officinalis (EEO) indicates its cardioprotective efficacy against rats fed with high fat diet.

scholarly journals The effect of high-fat diet and 13-cis retinoic acid application on lipid profile, glycemic response and oxidative stress in female Lewis rats

PLoS ONE ◽  
2020 ◽  
Vol 15 (9) ◽  
pp. e0238600
Author(s):  
Ivana Ilić ◽  
Nada Oršolić ◽  
Edi Rođak ◽  
Dyana Odeh ◽  
Marko Lovrić ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Retinoic Acid ◽  
Lipid Profile ◽  
High Fat Diet ◽  
Glycemic Response ◽  
High Fat ◽  
Lewis Rats ◽  
And Oxidative Stress

Molecular factors involved in the hypolipidemic- and insulin-sensitizing effects of a ginger (Zingiber officinale Roscoe) extract in rats fed a high-fat diet

2017 ◽  
Vol 42 (2) ◽  
pp. 209-215 ◽  
Author(s):  
Natalia de las Heras ◽  
María Valero-Muñoz ◽  
Beatriz Martín-Fernández ◽  
Sandra Ballesteros ◽  
Antonio López-Farré ◽  
...  
Keyword(s):  
Lipid Profile ◽  
Plasma Levels ◽  
High Fat Diet ◽  
Tissue Growth ◽  
Collagen I ◽  
Metabolic Effects ◽  
Control Group ◽  
Standard Diet ◽  
High Fat ◽  
Glucose Transporter 2

Hypolipidemic and hypoglycemic properties of ginger in animal models have been reported. However, information related to the mechanisms and factors involved in the metabolic effects of ginger at a hepatic level are limited. The aim of the present study was to investigate molecular factors involved in the hypoglycemic and hypolipidemic effects of a hydroethanolic ginger extract (GE) in the liver of rats fed a high-fat diet (HFD). The study was conducted in male Wistar rats divided into the following 3 groups: (i) Rats fed a standard diet (3.5% fat), the control group; (ii) rats fed an HFD (33.5% fat); and (iii) rats fed an HFD treated with GE (250 mg·kg−1·day−1) for 5 weeks (HFD+GE). Plasma levels of glucose, insulin, lipid profile, leptin, and adiponectin were measured. Liver expression of glycerol phosphate acyltransferase (GPAT), cholesterol 7 alpha-hydroxylase, peroxisome proliferator-activated receptors (PPAR), PPARα and PPARγ, glucose transporter 2 (GLUT-2), liver X receptor, sterol regulatory element-binding protein (SREBP1c), connective tissue growth factor (CTGF), and collagen I was measured. Data were analyzed using a 1-way ANOVA, followed by a Newman−Keuls test if differences were noted. The study showed that GE improved lipid profile and attenuated the increase of plasma levels of glucose, insulin, and leptin in HFD rats. This effect was associated with a higher liver expression of PPARα, PPARγ, and GLUT-2 and an enhancement of plasma adiponectin levels. Furthermore, GE reduced liver expression of GPAT, SREBP1c, CTGF, and collagen I. The results suggest that GE might be considered as an alternative therapeutic strategy in the management of overweight and hepatic and metabolic−related alterations.

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