scholarly journals Effectiveness of CBNAAT in the diagnosis of extrapulmonary tuberculosis

2018 ◽  
Vol 6 (12) ◽  
pp. 3925
Author(s):  
Shivprasad Kasat ◽  
Mahendra Biradar ◽  
Ashish Deshmukh ◽  
Sunil Jadhav ◽  
Hafiz Deshmukh
Keyword(s):  
Extrapulmonary Tuberculosis ◽  
Tertiary Care ◽  
Gastric Lavage ◽  
Clinical Problem ◽  
Turnaround Time ◽  
Nucleic Acid Amplification ◽  
Tertiary Care Centre ◽  
Cystic Fluid ◽  
Major Health Problem ◽  
Tuberculosis Patients

Background: Tuberculosis is still a major health problem worldwide. It is estimated that about one-third of the world's population is infected with mycobacterium tuberculosis. Whilepulmonary tuberculosis is most common presentation; extrapulmonary tuberculosis is also an important clinical problem. CBNAAT is cartridge based nucleic acid amplification test with a well-established role in the diagnosis of pulmonary tuberculosis (PTB). We determined the effectiveness of CBNAAT in the diagnosis of extrapulmonary tuberculosis (EPTB) cases in comparison to AFB smear.Methods: Retrospective study of suspected extrapulmonary tuberculosis patients in a tertiary care centre of the study area was conducted. The study period was from January 2017 to July 2018. Data of 166 consecutive suspected extrapulmonary tuberculosis patients was retrieved. Effectiveness of CBNAAT in the diagnosis of EPTB was assessed as compared to that of AFB smear.Results: Samples collected from 166 suspected EPTB patients were subjected to AFB smear and CBNAAT. Samples collected included lymph node, pus, pleural fluid, tissue, CSF, gastric lavage, cystic fluid, peritoneal fluid, ascitic fluid, colonic fluid, synovial fluid, urine. In AFB smear results, 17 cases were positive for TB bacilli and 149 were negative for the same. In CBNAAT results, 25 cases were positive for TB bacilli and 141 cases were negative. In comparative analysis, 8 cases were AFB smear negative but CBNAAT positive.Conclusions: CBNAAT is a useful tool in the diagnosis of EPTB cases because of its simplicity and rapid turnaround time. CBNAAT is more effective as compared to AFB smear in the diagnosis of EPTB cases.

A RETROSPECTIVE ANALYSIS OF EXTRAPULMONARY TUBERCULOSIS PATIENTS WITH INVOLVEMENT OF DIFFERENT ANATOMICAL SITES ATTENDING TERTIARY CARE CENTRE

2021 ◽  
pp. 35-36
Author(s):  
Ravinuthala Purnima ◽  
Hafiz Deshmukh ◽  
Ashish Deshmukh ◽  
Sunil Jadhav ◽  
Shiv Prasad Kasat ◽  
...  
Keyword(s):  
Tertiary Care Hospital ◽  
Extrapulmonary Tuberculosis ◽  
Tertiary Care ◽  
Age Groups ◽  
Anatomical Site ◽  
Care Hospital ◽  
Hospital Data ◽  
Tertiary Care Centre ◽  
Tuberculosis Patients ◽  
Medical College

OBJECTIVE: To assess the number of new cases of Extrapulmonary tuberculosis, by characterizing patients based on anatomical site. METHODS: This is a retrospective, descriptive study of diagnosed patients of EPTB of all age groups and was conducted in the MGM medical college and hospital, Aurangabad, a tertiary care hospital. Data for this study was obtained from JAN 2018 to DEC 2020. RESULTS: This study showed that out of all EPTB cases, the incidence of Lymph node T.B is higher followed by Pleural T.B. The incidence of EPTB is higher in younger population. CONCLUSION: Our study points out the population which is at higher risk of EPTB. Since diagnosing EPTB is difcult as the samples taken from the affected organs are paucibacillary giving rise to higher chance of misdiagnosing such patients. Astrong clinical suspicion plays a crucial role in the early diagnosis and treatment of such patients.

scholarly journals Diabetes mellitus and smoking among tuberculosis patients in a tertiary care centre in Karnataka, India

2013 ◽  
Vol 3 (1) ◽  
pp. 51-53 ◽  
Author(s):  
M. V. Jali ◽  
V. K. Mahishale ◽  
M. B. Hiremath ◽  
S. Satyanarayana ◽  
A. M. V. Kumar ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Tertiary Care ◽  
Care Centre ◽  
Tertiary Care Centre ◽  
Tuberculosis Patients

scholarly journals CLINICOPATHOLOGICAL STUDY OF CARCINOMA ORAL CAVITY & PATTERN OF METASTASIS- EXPERIENCE IN A TERTIARY CARE CENTRE

2020 ◽  
pp. 1-3
Author(s):  
Bijan Basak ◽  
Soutrik Kumar ◽  
Kaustuv Das Biswas ◽  
Sayan Hazra ◽  
Debarshi Jana
Keyword(s):  
Indian Subcontinent ◽  
Treatment Options ◽  
Tertiary Care ◽  
Neck Surgery ◽  
Tertiary Care Centre ◽  
Major Health Problem ◽  
Node Metastases ◽  
Long Term Prognosis ◽  
Head Neck Surgery ◽  
Head Neck

Oral cancer (code 145.9, ICD 9) encompasses all malignancies originating in oral tissues & it is a major health problem in many parts of the world. Although incidence is relatively low in the western countries, in the Indian subcontinent & other parts of Asia it remains one of the commonest cancers. The study was conducted on the patients attending the ENT & HEAD-NECK SURGERY OPD at the INSTITUTE OF OTORHINOLARYNGOLOGY & HEAD-NECK SURGERY, IPGME&R, Kolkata during a period of 1 year from 1st March 2019 to 29th February 2020. Buccal mucosa was the commonest site & most cases presented in late stage with cervical lymph node metastases entailing poorer prognosis. People should be made aware of the warning symptoms, need for early diagnosis & treatment options available through IEC (information, education & communication programmes) in order to provide better treatment outcomes, improved long term prognosis & thereby reducing the morbidity & mortality of people at large.

scholarly journals Utility of GeneXpert in Diagnosis of Multidrug-resistant Extrapulmonary Tuberculosis

2017 ◽  
Vol 03 (02) ◽  
pp. 085-087
Author(s):  
Ankush Chaudhary ◽  
Ketaki Utpat ◽  
Unnati Desai ◽  
Jyotsna Joshi
Keyword(s):  
Lymph Node ◽  
Psoas Abscess ◽  
Extrapulmonary Tuberculosis ◽  
Tertiary Care ◽  
False Negative ◽  
Multidrug Resistant ◽  
Nucleic Acid Amplification ◽  
Lower Sensitivity ◽  
Care Hospital ◽  
Gluteal Abscess

ABSTRACT Background: The Xpert Mycobacterium tuberculosis (MTB)/rifampicin (RIF) assay (GeneXpert) is a rapid semi-quantitative nucleic acid amplification test with established role in the diagnosis of pulmonary tuberculosis (PTB) and multidrug-resistant (MDR) PTB. We determined the performance of the GeneXpert assay for the diagnosis of extrapulmonary tuberculosis (EPTB) MDR cases. Aims and objectives: To study the role of GeneXpert in the diagnosis of EPTB MDR. Materials and methods: A retrospective study was conducted over a period of 2 years at a tertiary care hospital after Ethics Committee permission. Data of 44 consecutive patients of diagnosed EPTB MDR were retrieved for GeneXpert and culture drug susceptibility test (DST). Sensitivity of GeneXpert in the diagnosis of EPTB MDR was calculated comparing culture DST results. Results: Various EPTB MDR cases studied were lymph node TB (n = 23) 51%, pleural effusion (n = 14) 32%, central nervous system TB/spinal TB/psoas abscess/gluteal abscess (n = 7) 17%. Sensitivity for GeneXpert was found to be 91.30, 57, 100% respectively. True positive and false negative were 36 and 8 cases respectively. The overall sensitivity of GeneXpert in diagnosing EPTB MDR was 81.80%. The sensitivity among lymph node, pleural effusions, and spinal/psoas abscess/gluteal abscess was 91.30, 57.14, and 100% respectively. Conclusion: GeneXpert sensitivity for the diagnosis of EPTB MDR varied with site of extrapulmonary involvement, with lower sensitivity in pleural fluid as compared with higher sensitivity among lymph node and spine TB. Nevertheless, given the rapid turnaround time and simplicity, it is a useful tool in the diagnosis of EPTB MDR when used in correct clinical context. Subsequent confirmation with culture DST, however, is recommended to diagnose false negatives.

scholarly journals Optimization of Turnaround Time for Group A Streptococcus PCR

2019 ◽  
Vol 57 (9) ◽  
Author(s):  
Thomas J. S. Durant ◽  
Jacob Merwede ◽  
Jesse Reynolds ◽  
David R. Peaper
Keyword(s):  
Tertiary Care ◽  
Night Shift ◽  
Diagnostic Testing ◽  
Final Analysis ◽  
Turnaround Time ◽  
Nucleic Acid Amplification ◽  
Microbiology Laboratory ◽  
Workflow Optimization ◽  
Group A

ABSTRACT The use of some nucleic acid amplification tests (NAATs) for the diagnosis of group A Streptococcus (GAS) pharyngitis allows laboratories to adopt single-tiered testing without reflex culture. However, centralization may delay the delivery of actionable information to the bedside, particularly in the outpatient setting. We describe two novel workflows at our institution and their effect on in-lab turnaround time (TAT) at a tertiary care microbiology lab. Laboratory records were extracted, and relevant data were analyzed after the implementation of qualitative in vitro diagnostic testing for GAS with the Xpert Xpress Strep A assay, performed using the GeneXpert Infinity-48s. Workflow optimization steps studied included: (i) direct specimen submission to the microbiology laboratory via the pneumatic tube system and (ii) autoverification of GAS NAAT results in the laboratory information system. Between April 2018 and October 2018, 2,595 unique specimens were tested for GAS by PCR. Of these, 2,523 were included in the final analysis. Linear regression established that the total in-lab TAT was significantly reduced by direct specimen submission to the microbiology laboratory, autoverification, and processing during the night shift. We describe two workflow optimization methods that reduced the in-lab TAT for GAS NAAT. Although microbiology labs historically use manual processes, the advent of total laboratory automation and the adoption of on-demand NAATs will allow for more streamlined processing of microbiology specimens. It may be beneficial to consider instrument interfacing and specimen processing optimization during the early phases of implementation planning for NAATs in the microbiology laboratory.

scholarly journals Clinical profile of cases of dysphagia presenting in ENT department: a study from rural tertiary care center

2017 ◽  
Vol 3 (3) ◽  
pp. 639
Author(s):  
Ginni Datta ◽  
Manish Gupta ◽  
Naiya Rao
Keyword(s):  
Care Center ◽  
Tertiary Care ◽  
Clinical Problem ◽  
Needle Aspiration ◽  
Clinical Profile ◽  
Barium Swallow ◽  
Tertiary Care Centre ◽  
Predominant Symptom ◽  
Alarm Symptom ◽  
A Prospective Study

<p class="abstract"><strong>Background:</strong> Swallowing is a complex motor reflex requiring coordination among the neurologic system and muscles of the oropharynx and oesophagus. Disorders both benign and malignant may interfere with the process and cause dysphagia. We hereby undertake a study in a rural tertiary care centre to study the clinical profile of cases of dysphagia and to find the relative incidence of various etiologies of dysphagia.</p><p class="abstract"><strong>Methods:</strong> A prospective study was conducted upon both out and indoor patients coming to Department of Otorhinolaryngology from January 2016 to January 2017 with predominant symptom of difficulty in swallowing for both solids, liquids or either. Detailed history &amp; examination was done. Further endoscopy, barium swallow, fine needle aspiration cytology (FNAC) &amp; biopsies were done as required. A total of 140 cases were taken into consideration.  </p><p class="abstract"><strong>Results:</strong> The mean age was 52.5 years with 60% patients males and 40% females. The commonest etiology of dysphagia was Gastroesophageal reflux disease (GERD) occupying 28.57%of cases. Among them 65% werefemales majority in the age group of 45-55 years. The 2<sup>nd</sup> common cause of dysphagia was growth pharynx 18.5% of cases. Among them 19 cases were diagnosed as growth oropharynx and 7 cases as growth supraglottis extending to hypopharynx. 88.46% were males and all were smokers. The 3<sup>rd</sup> common etiology of dysphagia was obstructive oesophageal causes which included oesophageal malignancies, oesophageal webs, strictures and diverticula in the frequency of 16%, 3%, 3% and 6% respectively. Oesophageal malignancies were mainly adenocarcinoma, all males who were chronic smokers.</p><p><strong>Conclusions:</strong> Dysphagia is a commonly encountered clinical problem &amp; limited studies exist regarding the prevalence of dysphagia etiologies. It is an alarm symptom, malignancy should be ruled out, and warrants early intervention. </p>

scholarly journals Maternal outcome in thyroid dysfunction in admitted antenatal women in tertiary care centre: an observational study

2020 ◽  
Vol 9 (8) ◽  
pp. 3402
Author(s):  
Juhi Agarwal ◽  
Deepika Kesaraju ◽  
Poorva Badkur
Keyword(s):  
Health Problem ◽  
Thyroid Dysfunction ◽  
Tertiary Care ◽  
First Trimester ◽  
Health Care Facilities ◽  
Adverse Outcomes ◽  
Endocrine Disorders ◽  
Tertiary Care Centre ◽  
Major Health Problem ◽  
Gandhi Medical College

Background: Thyroid disorders constitute one of the most common endocrine disorders in pregnancy. Aim of this study was to identify thyroid dysfunction as a maternal health problem. Objective of this study was to study the maternal outcomes in these patients.Methods: The main source of data are the patients admitted in Sultania Zanana Hospital, GMC Bhopal. The study was an observational prospective study conducted from March 2018 to February 2019.Results: The present study was conducted in department of obstetrics and gynecology, Gandhi Medical College and associated Sultania Zanana Hospital Bhopal for the period of 1 year. The study could enroll 400 females fulfilling the inclusion criteria during the study period.Conclusions: In developing countries like India where there is prominent micronutrient deficiency, iodine deficiency further aggravates the condition and carries a great potential to show deleterious impacts on maternal and fetal outcomes, thus serving a major health problem. Considering the substantial increase in short term as well as long term perinatal morbidity, a felt need for creating awareness not only on adverse outcomes but also detection of the disorder in first trimester is required. The desired perinatal outcome can be achieved by early detection and prompt treatment of the disorder. Henceforth, universal screening of all antenatal women in the first trimester rather than targeted case finding should be recommended at all the health care facilities.

scholarly journals A Prospective Study of Clinical Profile in Febrile Illness with Thrombocytopenia in Children Aged 1 to 12 Years Admitted in a Tertiary Care Centre in Telangana

2021 ◽  
Vol 8 (35) ◽  
pp. 3247-3251
Author(s):  
Sreelatha Martha ◽  
Goutham Deeti ◽  
Chaitanya Jyothi Ravula ◽  
Nirmala Cherukuri ◽  
Srinivasa Suresh Nadavapalli
Keyword(s):  
Dengue Fever ◽  
Enteric Fever ◽  
Lymphoblastic Leukemia ◽  
Tertiary Care ◽  
Febrile Illness ◽  
Clinical Problem ◽  
Severe Thrombocytopenia ◽  
Tertiary Care Centre ◽  
Female Ratio ◽  
Gi Symptoms

BACKGROUND Fever with thrombocytopenia is a common clinical problem in paediatric wards. Significant number of acute febrile illnesses have an infectious aetiology and are often associated with thrombocytopenia. The objective of the study was to determine the clinico-etiological profile and outcome of children admitted with febrile thrombocytopenia, especially in those with infective aetiology. METHODS The study design is a prospective observational study. It was conducted from September 2017 to August 2019 in the Department of Paediatrics, Niloufer Institute of Women and Child Health, Hyderabad. A total of hundred (100) children in the age group of 1 year to 12 years presented with fever, and thrombocytopenia were included in the study. Newborns, infants, children with febrile thrombocytopenia, known ITP (idiopathic thrombocytopenic purpura), already diagnosed haematological malignancy and children on antiplatelet drugs like aspirin were excluded from the study. After informed written consent, detailed history was elicited, clinical examination and necessary laboratory investigations were carried out, and the data was captured in a pre-structured proforma. Study parameters were analysed using Statistical Package for Social Sciences (SPSS) version 16 software. RESULTS The study included 100 children. A ratio of 1.4 : 1 was observed in male to female ratio. As of the clinical features, gastrointestinal (GI) symptoms such as nausea, vomiting and pain abdomen were more common, followed by headache and myalgia. On examination, two-thirds of the children had hepatomegaly, and onethird had splenomegaly. Among 100 children with febrile thrombocytopenia, 38 children had bleeding manifestations (cutaneous bleeds > GI bleeds > other bleeds) in those with moderate to severe thrombocytopenia. In the etiological profile, dengue fever was more common, followed by undiagnosed fever, enteric fever, ALL (acute lymphoblastic leukemia), scrub typhus, malaria and leptospira, respectively. Out of 100 children, 94 were discharged, and 6 children with ALL were referred to the haemato-oncology center for further management. CONCLUSIONS Clinical presentation of cases with febrile thrombocytopenia is varied. Common causes of febrile thrombocytopenia observed in this study were dengue fever followed by un diagnosed fever and enteric fever. KEYWORDS Fever, Thrombocytopenia, Platelet count, Bleeding

Sign in / Sign up

Export Citation Format

Share Document