scholarly journals Utility of rapid diagnostic kit tests for diagnosis of suspected dengue fever

2019 ◽  
Vol 7 (5) ◽  
pp. 1670
Author(s):  
Sarita Otta ◽  
Bichitrananda Swain
Keyword(s):  
Platelet Count ◽  
Dengue Fever ◽  
Rapid Test ◽  
Febrile Illness ◽  
Serum Samples ◽  
Serological Tests ◽  
Ns1 Antigen ◽  
Elisa Technique ◽  
Low Platelet Count ◽  
Sensitivity Specificity

Background: Dengue fever often presents as an undifferentiated febrile illness requiring a laboratory test for identification. Serological tests particularly on rapid kits for the detection of NS1Antigen, IgG and IgM antibodies are the most commonly performed test across the country.Methods: The serum samples of suspected dengue cases were tested by Rapid test kits for assessing all the three parameters as well as by ELISA for NS1 antigen test. The platelet count of the patients was obtained from automated coulter counter. The results thus obtained were analyzed in Excel format.Results: The serum samples from 304 suspected Dengue fever cases were received in the lab, of which 190 samples were positive either by rapid or ELISA and 176 when rapid card test was considered alone Highest seropositivity of dengue cases were observed in the age group of ≥60 years (79.2%) followed by 45-59 years (70.7%). On rapid test, 78 cases were NS1 antigen positive of which 60 cases were positive only for NS1 antigen. When NS1 rapid and ELISA tests when compared, 16 kit negative tests were positive on ELISA while 34 kit positive tests were ELISA negative.  Sensitivity, specificity, PPV and NPV when only NS1 was considered on rapid test kits when compared with ELISA were 78.9%, 87.8%, 63.8% and 93.8%. 33.5% of serologically positive cases of Dengue had low platelet count on admission while only among negative cases 17.2% had a low platelet.Conclusions: Rapid kits often show variable results thus needing a validation of them from end user. As a positive dengue test result is an essential prerequisite for diagnosis thus it is essential that for serological tests ELISA technique should be used for reporting. Thus, it also mandates more efforts at decentralization of NVBDCP to include both government and non government institutions.

scholarly journals Serum Ferritin: A Backstage Weapon in Diagnosis of Dengue Fever

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Soumyabrata Roy Chaudhuri ◽  
Subhayan Bhattacharya ◽  
Mainak Chakraborty ◽  
Kingshuk Bhattacharjee
Keyword(s):  
Platelet Count ◽  
Retrospective Study ◽  
Dengue Fever ◽  
Serum Ferritin ◽  
Ferritin Level ◽  
Dengue Infection ◽  
Surrogate Marker ◽  
Febrile Illness ◽  
Dengue Outbreak ◽  
Ns1 Antigen

Aims. This retrospective study evaluates ferritin as a surrogate marker for dengue infection (NS1 and IgM negative stage) as opposed to other febrile illnesses of infective or inflammatory etiology (OFI). Methodology. Data of all patients admitted to medical ward and medical ITU during the dengue outbreak were collected. Patients admitted between 5 and 10 days of febrile illness without a diagnosis were included. Patients with NS1 positivity (Days 2–8) and/or positive IgM for dengue (Days 6–10) were considered to be dengue cases and those with other confirmed diagnoses were considered in the OFI group. Ferritin, CRP, TC of WBC, platelet count, SGOT, SGPT, and albumin levels were analysed for both groups. Results. We examined 30 cases of clinically and serologically confirmed dengue fever and 22 cases of OFI. Ferritin level in dengue cohort was significantly higher than the OFI group (p<0.0001). The best cut-off for ferritin level to differentiate dengue from OFI was found to be 1291. The sensitivity at this cut-off is 82.6% and the specificity at this cut-off is 100%. Conclusion. Ferritin may serve as a significant marker for differentiating between dengue fever and OFI, in absence of a positive NS1 antigen or a positive IgM antibody for dengue.

scholarly journals Chikungunya Fever in Southern Thailand, 2018

2021 ◽  
Author(s):  
Natthaphon Nanakorn ◽  
Theerakamol Pengsakul ◽  
Kannika Bunrod ◽  
Suwich Thammapalo ◽  
Pathomporn Prikchoo ◽  
...  
Keyword(s):  
South African ◽  
Rapid Test ◽  
Febrile Illness ◽  
Positive Sequence ◽  
Rt Pcr ◽  
Serum Samples ◽  
Serological Tests ◽  
Recent Infection ◽  
Southern Thailand ◽  
The Indian Ocean

Infection by the mosquito-borne chikungunya virus (CHIKV) causes acute febrile illness and debilitating arthralgia. Outbreaks are sometimes not recognized because of its clinical resemblance to the more common dengue fever ubiquitous in tropical countries. An upsurge of dengue-like illness was reported in Satun province located in southern Thailand during the rainy season in 2018. We investigated probable outbreak of CHIKV disease. We collected serum samples from 127 patients and tested for CHIKV infection based on nucleic acid and serological tests. CHIKV RNA amplified by real-time reverse-transcription polymerase chain reaction (RT-PCR) and IgM antibody against CHIKV were determined by immunochromatographic rapid test. Mosquitoes in the community were also trapped and tested for CHIKV. Conventional RT-PCR on initially positive samples was performed to obtain nucleotide sequences for subsequent phylogenetic analysis. In all, 39% (50/127) of the samples tested positive for CHIKV RNA, IgM, or both. Of these, CHIKV RNA was identified in 17% (21/127) of the samples. Fourteen percent (18/127) of the samples were simultaneously positive for both IgM and IgG, which suggest recent infection. One sample tested positive for both CHIKV IgM and RNA. Several samples from Aedes aegypti and Aedes albopictus mosquitoes were also CHIKV RNA-positive. Sequence analysis revealed that the Satun CHIKV belonged to the Indian Ocean lineage within the East/Central/South African (ECSA) clade with residues K211E and A226 in the E1 gene, and G205S and V264A in the E2 gene. The ECSA strain of CHIKV continues to evolve and possesses virulent potential despite causing prior outbreaks in the region.

A PROSPECTIVE STUDY ON VARIOUS SEROLOGICAL MARKERS AND PLATELET COUNT IN DENGUE INFECTION IN AND AROUND KANCHEEPURAM.

2021 ◽  
pp. 45-46
Author(s):  
Hamsadwani K P ◽  
Sneka P ◽  
Sangamithra V
Keyword(s):  
Platelet Count ◽  
Dengue Infection ◽  
Febrile Illness ◽  
Antibody Detection ◽  
Serological Markers ◽  
Serum Samples ◽  
Platelet Counts ◽  
Acute Viral Infection ◽  
Ns1 Antigen ◽  
A Prospective Study

INTRODUCTION: Dengue is an acute viral infection with potential fatal complications, presenting with non-specic fever that mimics other febrile illness. Specic antibody detection has been the mainstay of diagnosis which is prone for false positive and negative reactions. The newer parameter NS1 appears to be highly specic and reliable for diagnosis. Therefore, we tried to evaluate the association of platelet counts against NS1 and IgM / IgG in dengue infections. OBJECTIVE: To study the association of thrombocytopenia with NS1 antigen and specic antibodies in dengue infection in kancheepuram population. MATERIALS AND METHODS: Serum samples from clinically suspected dengue cases were tested for NS1,IgM,IgG by immunochromatography – based test. Platelet counts were obtained from all positive cases. Test results of dengue-specic parameters and platelet counts were compared. RESULTS: Of the 612 samples tested, 150 were positive for one or more dengue serological markers. Of the 150, 27(18%) were positive for NS1only, 45(30%)were positive for IgM, 54(36%) were positive for IgG only. Of the 150, 39(26%) showed platelet count less than 1 lakh. CONCLUSION: Inclusion of NS1 in the diagnosis of dengue increases the detection and specicity rate. Thus correlation of platelet count, NS1,IgM and ,IgG gives detailed picture of the stage and prognosis of the disease

scholarly journals Emergence and Autochthonous Transmission of Dengue Virus Type I in a Low-Epidemic Region in Southeast China

2021 ◽  
Vol 11 ◽  
Author(s):  
Yi Zhang ◽  
Hongyi Chen ◽  
Jingen Wang ◽  
Shumei Wang ◽  
Jing Wu ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Dengue Fever ◽  
Rapid Test ◽  
Febrile Illness ◽  
Severe Dengue ◽  
Type I ◽  
Dengue Outbreaks ◽  
Ns1 Antigen ◽  
And Control ◽  
Autochthonous Transmission

BackgroundDengue fever is a mosquito-borne febrile illness. Southeast Asia experienced severe dengue outbreaks in 2019, and over 1000 cases had been reported in Jiangxi, a previously known low-epidemic region in China. However, the emergence of a dengue virus epidemic in a non-epidemic region remains unclear.MethodsWe enrolled 154 dengue fever patients from four hospitals in Jiangxi, from April 2019 to September 2019. Real-time PCR, NS1 antigen rapid test, and IgM, IgG tests were performed, and 14 samples were outsourced to be sequenced metagenomically.ResultsAmong the 154 cases, 42 were identified as imported and most of them returned from Cambodia. A total of 113 blood samples were obtained and 106 were identified as DENV-1, two as DENV-2, and five were negative through RT-PCR. All DENV-1 strains sequenced in this study were all classified to one cluster and owned a high similarity with a Cambodia strain isolated in 2019. The evolutionary relationships of amino acid were consistent with that of nucleotide genome result. The sequence-based findings of Jiangxi strains were consistent with epidemiological investigation.ConclusionEpidemiological analysis demonstrated that the emergence of dengue cases led to autochthonous transmission in several cities in Jiangxi, a low-epidemic region before. This study emphasized future prevention and control of dengue fever in both epidemic and non-epidemic regions.

scholarly journals Proportion and Clinical Outcome of Bleeding Manifestations in Confirmed Dengue Fever Cases – A Descriptive Longitudinal Study from Ernakulam, Kerala, India

2021 ◽  
Vol 10 (26) ◽  
pp. 1921-1925
Author(s):  
Amrish Thannickal Thazhath ◽  
John Sajith ◽  
Jacob K. Jacob ◽  
George Jilse ◽  
Samuel Suma ◽  
...  
Keyword(s):  
Platelet Count ◽  
Dengue Fever ◽  
Tertiary Care ◽  
Age Groups ◽  
Previous History ◽  
Study Data ◽  
Medical College ◽  
Ns1 Antigen ◽  
History Of ◽  
Low Platelet Count

BACKGROUND Dengue fever is expanding in newer areas globally, with greater morbidity and mortality. We wanted to determine the proportion of bleeding manifestations among the confirmed dengue fever patients and evaluate the clinical outcomes in those with bleeding manifestations. METHODS 230 Dengue confirmed patients admitted in Government Medical College, Ernakulam from January 2018 to December 2018 were enrolled for the study. Data was recorded and entered in Microsoft Excel, and analysed using SPSS. RESULTS In the current study out of 230 subjects, bleeding manifestations were more common in 21 - 30 & 41 - 50 age groups. Out of 230 subjects, 102 had bleeding manifestations (44.3 %) like petechiae (34 %), purpura (24 %), echymosis (19.1 %), gum bleeding (17.8 %), menorrhagia (10.9 %), melena (10.4 %), conjunctival haemorrhage (9.1 %), haematuria (4.8 %), epistaxis (3.5 %), haemoptysis (2.2 %) and hematemesis (0.9 %). 86 subjects (37.4 %) received platelet transfusion on an average of 4 units. Age group, fever, previous history of dengue, IgG positivity, NS1 positivity were significantly (P < 0.0001) associated with bleeding. Most of the bleeding manifestations were significant in patients who had lower platelet counts on the first day of admission when it was less than ten thousand. CONCLUSIONS 44.3 % had bleeding manifestations with significant association between age, fever and IgG positivity. A low platelet count on admission especially less than 10,000 was significantly associated with these bleeding manifestations. These significant observations are important for both the primary care and tertiary care physicians in meticulous management and referral of dengue patients. KEY WORDS Dengue Fever, Bleeding Manifestations, Ns1 Antigen, Platelet Count.

scholarly journals Investigation of the rapid immunochromatographic test performance in the diagnosis of syphilis; comparison of four serological methods

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Huseyin Agah Terzi ◽  
Ozlem Aydemir ◽  
Engin Karakece ◽  
Huseyin Hatipoglu ◽  
Mehmet Olmez ◽  
...  
Keyword(s):  
Test Performance ◽  
Gold Standard ◽  
Treponema Pallidum ◽  
Rapid Test ◽  
Immunochromatographic Test ◽  
Rapid Plasma Reagin ◽  
Serum Samples ◽  
Hemagglutination Assay ◽  
Predictive Values ◽  
Sensitivity Specificity

AbstractObjectivesTo test the performance of the newly available rapid test for syphilis, we compared it with Treponema pallidum hemagglutination assay (TPHA). Additionally, we investigated the performance of rapid plasma reagin (RPR) and chemiluminescence microparticle immunoassays (CMIA) at our laboratory using TPHA as a gold standard.MethodsThe serum samples of 595 patients with the pre-diagnosis of syphilis were studied by four serological methods. The sensitivity, specificity, and predictive values of RPR, CMIA, and syphilis rapid test were assessed by utilizing TPHA as a gold standard for the diagnosis of syphilis.ResultsOf the patients, 6.2% (37/595) had positive RPR, 5.5% (33/595) had positive CMIA, 5.5% (33/595) had a positive rapid immunochromatographic method and 5% (30/595) had positive TPHA. When TPHA results were taken as the reference, the sensitivity of the rapid test for syphilis was 100%, the specificity was 99.5%, PPV was 90.9%, and NPV was 100.0%.ConclusionsIt was observed that the rapid test for syphilis used in the study was quite successful, its cost was appropriate, and the test was very fast and easy to apply. At the same time, the agreement between syphilis rapid test and TPHA was found to be excellent.

scholarly journals Ethylenediaminetetraacetic Acid‑Dependent Pseudothrombocytopenia in Patient Previously Treated as Dengue Fever

2021 ◽  
Vol 17 (1) ◽  
pp. 65
Author(s):  
Hendra Wana Nur’amin ◽  
Muhammad Darwin Prenggono ◽  
Wivina Riza Devi
Keyword(s):  
Bone Marrow ◽  
Platelet Count ◽  
Dengue Fever ◽  
Complete Blood Count ◽  
Ethylenediaminetetraacetic Acid ◽  
Bone Marrow Aspiration ◽  
Normal Platelet ◽  
Low Platelet Count ◽  
Bleeding Manifestation ◽  
Secondary Hospital

Abstract: One of the most widely used anticoagulants for a complete blood count is ethylenediaminetetraacetic acid (EDTA).  Pseudothrombocytopenia (PTCP) may be caused by EDTA, this condition may lead to inappropriate diagnosis and treatment. We report a 25-year-old female with unspecific headache and joint pain with very low platelet count since 1 month before hospital admission. She was diagnosed with Dengue fever infection and got some platelet transfusion from the previous secondary hospital. She was carried out for a blood test with another anticoagulant (sodium citrate) and bone marrow aspiration. The results showed that she had normal platelet count and bone marrow cellularity. When a patient was identified with thrombocytopenia without any bleeding manifestation, hematology disease, and family history, PTCP should be taken into consideration to prevent unnecessary intervention. Keywords: platelet, pseudothrombocytopenia, ethylenediaminetetraacetic acid, Dengue fever

Maiden outbreaks of dengue virus 1 genotype III in rural central India

2014 ◽  
Vol 143 (2) ◽  
pp. 412-418 ◽  
Author(s):  
P. V. BARDE ◽  
B. K. KORI ◽  
M. K. SHUKLA ◽  
P. K. BHARTI ◽  
G. CHAND ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Phylogenetic Analyses ◽  
Dengue Infection ◽  
Central India ◽  
Febrile Illness ◽  
Control Measures ◽  
Serum Samples ◽  
Serological Tests ◽  
Genotype Iii ◽  
Virus Serotype

SUMMARYDengue is regarded as the most important arboviral disease. Although sporadic cases have been reported, serotypes responsible for outbreaks have not been identified from central India over the last 20 years. We investigated two outbreaks of febrile illness, in August and November 2012, from Korea district (Chhattisgarh) and Narsinghpur district (Madhya Pradesh), respectively. Fever and entomological surveys were conducted in the affected regions. Molecular and serological tests were conducted on collected serum samples. Dengue-specific amplicons were sequenced and phylogenetic analyses were performed. In Korea and Narsinghpur districts 37·3% and 59% of cases were positive, respectively, for dengue infection, with adults being the worst affected. RT–PCR confirmed dengue virus serotype 1 genotype III as the aetiology. Ninety-six percent of infections were primary. This is the first time that dengue virus 1 outbreaks have been documented from central India. Introduction of the virus into the population and a conducive mosquitogenic environment favouring increased vector density caused the outbreak. Timely diagnosis and strengthening vector control measures are essential to avoid future outbreaks.

scholarly journals Evaluation of the performance of three serological tests for diagnosis of Leishmania infantum infection in dogs using latent class analysis

2020 ◽  
Vol 29 (4) ◽  
Author(s):  
Asier Basurco ◽  
Alda Natale ◽  
Katia Capello ◽  
Antonio Fernández ◽  
María Teresa Verde ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Leishmania Infantum ◽  
Latent Class ◽  
Rapid Test ◽  
Antibody Test ◽  
Latent Class Models ◽  
Canine Leishmaniasis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Serological Tests

Abstract Canine leishmaniasis (CanL) is a disease caused by Leishmania infantum. Serological methods are the most common diagnostic techniques used for the diagnosis of the CanL. The objective of our study was to estimate the sensitivity and specificity of one in-house ELISA kit (ELISA UNIZAR) and three commercially available serological tests (MEGACOR Diagnostik GmbH) including an immunochromatographic rapid test (FASTest LEISH®), an immunofluorescent antibody test (MegaFLUO LEISH®) and an enzyme-linked immunosorbent assay (MegaELISA LEISH®), using latent class models in a Bayesian analysis. Two hundred fifteen serum samples were included. The highest sensitivity was achieved for FASTest LEISH® (99.38%), ELISA UNIZAR (99.37%), MegaFLUO LEISH® (99.36%) followed by MegaELISA LEISH® (98.49%). The best specificity was obtained by FASTest LEISH® (98.43%), followed by ELISA UNIZAR (97.50%), whilst MegaFLUO LEISH® and MegaELISA LEISH® obtained the lower specificity (91.94% and 91.93%, respectively). The results of present study indicate that the immunochromatographic rapid test evaluated FASTest LEISH® show similar levels of sensitivity and specificity to the quantitative commercial tests. Among quantitative serological tests, sensitivity and specificity were similar considering ELISA or IFAT techniques.

scholarly journals Comparison of NS1 Antigen Detection by Rapid Immunochromatography Test and ELISA for Early Diagnosis of Dengue at a Government General Hospital, Ananthapuramu

2021 ◽  
pp. 378-381
Author(s):  
Adireddi Paradesi Naidu ◽  
Chitralekha Saikumar ◽  
G. Sumathi ◽  
Kalavathy Victor ◽  
N. S. Muthiah
Keyword(s):  
Rapid Test ◽  
Dengue Shock Syndrome ◽  
Hemorrhagic Fever ◽  
Clinical History ◽  
Elisa Test ◽  
Microbiology Laboratory ◽  
Serum Samples ◽  
Test Sensitivity ◽  
Ns1 Antigen ◽  
History Of

Background: The incidence of Dengue hemorrhagic fever, Dengue shock syndrome associated with Dengue can be reduced by diagnosing Dengue early and by initiating early treatment to Dengue patients. This study was conducted to compare results of NS1 antigen rapid test and ELISA in clinically suspected dengue patients. Materials and Methods: Present study was a comparative study conducted on 100 Patients presented with clinical history of Dengue. At Microbiology Laboratory, serum of all samples was assessed for NS1 detection using antigen Rapid test and ELISA.  Sensitivity & specificity values were calculated for NS1 antigen rapid test, compared with ELISA. Results: Out of 100 serum samples collected from suspected cases of Dengue in and around Anantapuramu, 30 (30%) were positive for ELISA and 28 (28%) were positive for Rapid diagnostic test. Sensitivity & specificity when only NS1 was considered on rapid test kits when compared with ELISA were 93.33%, 98.57%, Conclusion: It is concluded that ELISA test was superior in the diagnosis of Dengue and recommended on improvement in sensitivity of RDTs.

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