scholarly journals Comparison of NS1 Antigen Detection by Rapid Immunochromatography Test and ELISA for Early Diagnosis of Dengue at a Government General Hospital, Ananthapuramu

2021 ◽  
pp. 378-381
Author(s):  
Adireddi Paradesi Naidu ◽  
Chitralekha Saikumar ◽  
G. Sumathi ◽  
Kalavathy Victor ◽  
N. S. Muthiah
Keyword(s):  
Rapid Test ◽  
Dengue Shock Syndrome ◽  
Hemorrhagic Fever ◽  
Clinical History ◽  
Elisa Test ◽  
Microbiology Laboratory ◽  
Serum Samples ◽  
Test Sensitivity ◽  
Ns1 Antigen ◽  
History Of

Background: The incidence of Dengue hemorrhagic fever, Dengue shock syndrome associated with Dengue can be reduced by diagnosing Dengue early and by initiating early treatment to Dengue patients. This study was conducted to compare results of NS1 antigen rapid test and ELISA in clinically suspected dengue patients. Materials and Methods: Present study was a comparative study conducted on 100 Patients presented with clinical history of Dengue. At Microbiology Laboratory, serum of all samples was assessed for NS1 detection using antigen Rapid test and ELISA.  Sensitivity & specificity values were calculated for NS1 antigen rapid test, compared with ELISA. Results: Out of 100 serum samples collected from suspected cases of Dengue in and around Anantapuramu, 30 (30%) were positive for ELISA and 28 (28%) were positive for Rapid diagnostic test. Sensitivity & specificity when only NS1 was considered on rapid test kits when compared with ELISA were 93.33%, 98.57%, Conclusion: It is concluded that ELISA test was superior in the diagnosis of Dengue and recommended on improvement in sensitivity of RDTs.

scholarly journals Utility of rapid diagnostic kit tests for diagnosis of suspected dengue fever

2019 ◽  
Vol 7 (5) ◽  
pp. 1670
Author(s):  
Sarita Otta ◽  
Bichitrananda Swain
Keyword(s):  
Platelet Count ◽  
Dengue Fever ◽  
Rapid Test ◽  
Febrile Illness ◽  
Serum Samples ◽  
Serological Tests ◽  
Ns1 Antigen ◽  
Elisa Technique ◽  
Low Platelet Count ◽  
Sensitivity Specificity

Background: Dengue fever often presents as an undifferentiated febrile illness requiring a laboratory test for identification. Serological tests particularly on rapid kits for the detection of NS1Antigen, IgG and IgM antibodies are the most commonly performed test across the country.Methods: The serum samples of suspected dengue cases were tested by Rapid test kits for assessing all the three parameters as well as by ELISA for NS1 antigen test. The platelet count of the patients was obtained from automated coulter counter. The results thus obtained were analyzed in Excel format.Results: The serum samples from 304 suspected Dengue fever cases were received in the lab, of which 190 samples were positive either by rapid or ELISA and 176 when rapid card test was considered alone Highest seropositivity of dengue cases were observed in the age group of ≥60 years (79.2%) followed by 45-59 years (70.7%). On rapid test, 78 cases were NS1 antigen positive of which 60 cases were positive only for NS1 antigen. When NS1 rapid and ELISA tests when compared, 16 kit negative tests were positive on ELISA while 34 kit positive tests were ELISA negative.  Sensitivity, specificity, PPV and NPV when only NS1 was considered on rapid test kits when compared with ELISA were 78.9%, 87.8%, 63.8% and 93.8%. 33.5% of serologically positive cases of Dengue had low platelet count on admission while only among negative cases 17.2% had a low platelet.Conclusions: Rapid kits often show variable results thus needing a validation of them from end user. As a positive dengue test result is an essential prerequisite for diagnosis thus it is essential that for serological tests ELISA technique should be used for reporting. Thus, it also mandates more efforts at decentralization of NVBDCP to include both government and non government institutions.

scholarly journals Antibodies against canine distemper virus, parvovirus and Ehrlichia spp. in wild captive carnivores in midwestern Brazil

2018 ◽  
Vol 38 (8) ◽  
pp. 1681-1684
Author(s):  
Isis I.G.G. Taques ◽  
Thaís O. Morgado ◽  
Ísis A. Braga ◽  
Regina C.R. Paz ◽  
Sandra H.R. Corrêa ◽  
...  
Keyword(s):  
Canine Distemper Virus ◽  
Clinical History ◽  
Neutralization Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Canine Distemper ◽  
Serum Samples ◽  
Virus Neutralization Assay ◽  
Hemagglutination Inhibition Test ◽  
History Of ◽  
Indirect Immunofluorescent

ABSTRACT: The occurrence of antibodies against canine distemper virus (CDV), parvovirus and Ehrlichia spp. in wild captive carnivores was evaluated in a zoological park in midwestern Brazil. Serum samples were collected between 2007 and 2014 from 45 carnivores. Antibodies were evaluated by virus neutralization assay for CDV, hemagglutination inhibition test for parvovirus, indirect immunofluorescent and Enzyme-linked immunosorbent assay for Ehrlichia spp. Antibodies against CDV and parvovirus were detected in 75% of Canidae and Felidae. Procyonidae were negative for CDV, although one Mustelidae was positive. TwoCanidae presented antibodies reactive to E. canis antigens. The high antibodies rates to CDV and parvovirus suggest the contact with both pathogens, however since no clinical history of disease are registered in the Zoo-UFMT, we can presume that carnivores have responded satisfactorily against the antigens. The low serological rates observed against Ehrlichia spp. may be resulted to the low occurrence of ticks among carnivores.

scholarly journals Comparative Analysis of the Serological Reactivity of Individuals with Clinical History of Malaria using Two Different ELISA Tests

Diagnostics ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 168
Author(s):  
Yorleydy Ruiz Moreno ◽  
Silvia Tavares Donato ◽  
Fátima Nogueira ◽  
Marcelo Sousa Silva
Keyword(s):  
Clinical History ◽  
Negative Control ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serological Response ◽  
Serum Samples ◽  
Serological Tests ◽  
Serological Reactivity ◽  
History Of ◽  
Low Levels ◽  
Antigenic Reactivity

Early diagnosis of malaria reduces disease, prevents deaths, and contributes to decreased malaria transmission. The use of specific and sensitive antigens in the execution of serological diagnostics may have an impact on the transmission of the disease. However, many individuals cannot be easily diagnosed by serological tests due to low levels of antibodies in the serum. Using two different Enzyme-Linked Immunosorbent Assay (ELISA) tests (a commercial and an in-house ELISA), a total of 365 serum samples from individuals with a clinical history of malaria were analyzed. From the serum samples analyzed, 192 (53%) samples from the commercial ELISA and 219 (60%) samples from the in-house ELISA presented positive serological reactivity to malaria. The concordance of the samples tested (n = 365) between both ELISAs was of 67% (n = 242), and with the negative control was 100% (n = 17). We demonstrated that the in-house ELISA showed high antigenic reactivity to Plasmodium falciparum antigens when compared with the commercial ELISA. The degree of concordance of both ELISAs suggested the possibility of existence of other P. falciparum antigens present in the crude extract of P. falciparum that are important in the serological response during malaria infection.

scholarly journals Reproductive Disorders and Seroprevalence of Brucellosis in Yak

2014 ◽  
Vol 8 ◽  
pp. 130-132 ◽  
Author(s):  
Sitaram Aryal ◽  
Krishna P. Paudel
Keyword(s):  
Adult Female ◽  
Brucella Abortus ◽  
Random Sampling ◽  
Elisa Test ◽  
Reproductive Disorders ◽  
Serum Samples ◽  
Retained Placenta ◽  
History Of ◽  
And Control

A study on reproductive disorders among yaks in Mustang and Solukhumbu districts and seroprevalence of brucellosis among yaks in Mustang and Myagdi districts was conducted. Eleven farmers having 383 female and 72 male yaks in Mustang and 129 female and 27 male yaks in Solukhumbu districts were taken by purposive random sampling for the study on reproductive disorders. Likewise Sixty-seven serum samples were also collected from adult female yaks having history of abortion and retained placenta, and from male yaks used for breeding purpose from Mustang and Myagdi districts. An average of 12.63% of female yaks aborted, 7.26% had had retention of placenta and 6.6% repeat breeders in Mustang district. Similarly, 6.3% had abortion, 2.09% retained placenta and 6.5% repeat breeders in Solukhumbu district. However, all the 67 serum samples examined were found negative to the Brucella abortus antibody on ELISA test suggesting that the yak population was free from brucellosis in these areas. Thus abortion and retention of placenta in Yak in Mustang district may not be due to brucellosis. A detail study should be done to find the specific cause of abortion and repeat breeding to recommend preventive and control measures.Nepal Agric. Res. J. Vol. 8, 2007, pp. 130-132 DOI: http://dx.doi.org/10.3126/narj.v8i0.11608

scholarly journals CLINICAL AND HEMATOLOGICAL PROFILE OF PATIENTS WITH DENGUE FEVER AT A TERTIARY CARE HOSPITAL – AN OBSERVATIONAL STUDY

2018 ◽  
Vol 10 (1) ◽  
pp. e2018021 ◽  
Author(s):  
Vishal Vishnu Tewari ◽  
Kunal Tewari ◽  
Ritu Mehta
Keyword(s):  
Dengue Fever ◽  
Tertiary Care ◽  
Dengue Shock Syndrome ◽  
Hemorrhagic Fever ◽  
Annual Data ◽  
Care Hospital ◽  
Igg Antibody ◽  
Hematological Profile ◽  
Ns1 Antigen ◽  
Duration Of Hospitalization

AbstractBackground: Dengue is a major health issue with seasonal rise in dengue fever cases imposing an additional burden on hospitals, necessitating bolstering of services in the emergency department, laboratory with creation of additional dengue fever wards.Objectives: To study the clinical and hematological profile of dengue fever cases presenting to a hospital.Methods: Patients with fever and other signs of dengue with either positive NS1 antigen test or IgM or IgG antibody were included. Age, gender, clinical presentation, platelet count and hematocrit were noted and patients classified as dengue fever (DF), dengue hemorrhagic fever (DHF) or dengue shock syndrome (DSS). Duration of hospitalization, bleeding manifestations, requirement for platelet component support and mortality were recorded.Results: There were 443 adults and 57 children between 6 months to 77 year age. NS1 was positive in 115 patients (23%). Fever (99.8%) and severe bodyache (97.4%) were the commonest presentation. DF was seen in 484 (96.8 %), DHF in 10 (2%) and DSS in 6 cases (1.2%). OPD treatment was needed in 412 (82%) and hospitalization in 88 (18%). Intravenous fluid resuscitation was needed in 16 (3.2%) patients. Thrombocytopenia was seen in 335 (67%) patients at presentation. Platelet transfusion was needed in 46 (9.2%). PRC transfusion was given in 3 patients with DF and 10 of DHF. Death occurred in 03 DSS and 2 DHF patients. Conclusions: Majority of DF cases can be managed on OPD basis. DHF and DSS carry high mortality. Hospitals can analyze annual data for resource allocation for capacity expansion.

scholarly journals A Human Case of Lumbosacral Canal Sparganosis in China

2021 ◽  
Vol 59 (6) ◽  
pp. 635-638
Author(s):  
Jian-Feng Fan ◽  
Sheng Huang ◽  
Jing Li ◽  
Ren-Jun Peng ◽  
He Huang ◽  
...  
Keyword(s):  
Human Case ◽  
Elisa Test ◽  
Hunan Province ◽  
Surgical Removal ◽  
Lumbosacral Spine ◽  
Serum Samples ◽  
Gadolinium Contrast ◽  
Inferior Margin ◽  
History Of ◽  
Magnetic Resonance Imaging Mri

In this study, we intended to describe a human case of lumbosacral canal sparganosis in People’s Republic of China (China). A 56-year-old man was admitted to Xiangya Hospital Central South University in Changsha, Hunan province, China after having an experience of perianal pain for a week. An enhancing mass, a tumor clinically suggested, was showed at the S1-S2 level of the lumbosacral spine by the examination of magnetic resonance imaging (MRI) with gadolinium contrast. The patient was received the laminectomy from S1 to S2, and an ivory-white living worm was detected in inferior margin of L5. In ELISA-test with cerebrospinal fluid (CSF) and serum samples, anti-sparganum antibodies were detected. He had a ingesting history of undercooked frog meat in his youth. By the present study, a human case of spinal sparganosis invaded in lumbosacral canal at the S1-S2 level was diagnosed in China. Although the surgical removal of larvae is known to be the best way of treatment for sparganosis, we administered the high-dosage of praziquantel, albendazole and dexamethasone to prevent the occurrence of another remain worms in this study.

scholarly journals Risk factors related to seropositivity for Leptospira spp. in horses in the Serra Catarinense region

2020 ◽  
Vol 50 (7) ◽  
Author(s):  
Deise Keli Farias ◽  
Gabriela Dick ◽  
Silvério Bunn ◽  
André Thaler Neto ◽  
Carolina Rech ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Risk Factors ◽  
Animal Species ◽  
Clinical History ◽  
Control Measures ◽  
Serum Samples ◽  
Factors Associated ◽  
Leptospira Spp ◽  
History Of ◽  
And Control

ABSTRACT: Leptospirosis is a zoonotic infectious disease caused by the bacterium Leptospira spp. The consequences of infection in horses are unclear, and the serogroups involved vary depending on the region. This study aimed to evaluate the seropositivity of unvaccinated horses in the Serra Catarinense region and the possible risk factors associated with the infection. We used 207 horses from 26 properties, with no clinical history of infection and unvaccinated for leptospirosis. We submitted serum samples to the microscopic agglutination serum (SAM) to obtain the main reference serogroups. We considered animals with titre ≥100 as infected. The results of the serology revealed seropositivity in 45.4% (94/207) of the animals studied. The most frequent antibodies reportedwere those against the serogroups Australis 16.9% (35/207), Ichterhaemorrhagiae 14.4% (30/207), and Grippotyphosa 5.31% (11/207). Among the primary risk factors associated with the infection we reported extensive rearing system (OR = 1.27; P<0.05) and the presence of other animal species such as cattle (OR = 3.85; P<0.01) and capybaras (OR = 2.07; P=0.06). The findings presented in this study showed that leptospirosis is endemic in horses in the Serra Catarinense region, and revealed the need for emergency surveillance and control measures specific to this important zoonosis.

scholarly journals Seroprevalence of Dengue Infection in a Tertiary Care Hospital in Assam

2018 ◽  
Vol 7 (1) ◽  
pp. 1582 ◽  
Author(s):  
Liza Goswami ◽  
Chowdhury Runumi ◽  
Elmy S. Rasul
Keyword(s):  
Dengue Infection ◽  
Tertiary Care ◽  
Age Groups ◽  
Hemorrhagic Fever ◽  
Health Concern ◽  
Care Hospital ◽  
Serum Samples ◽  
Igm Antibody ◽  
Ns1 Antigen ◽  
Antibody Elisa

<p><strong>Background:</strong> Dengue fever (DF) and dengue hemorrhagic fever (DHF) are important arthropod borne viral diseases. Dengue in India has dramatically expanded over the last few decades, with rapidly changing epidemiology. Dengue is emerging as major public health concern in northeast India and spreading with increased morbidity.</p><p><strong>Objective:</strong>This study was carried out to determine the seroprevalence of Dengue infection among patients attending in Fakhruddin Ali Ahmed Medical College and Hospital, Barpeta, Assam during the period 2013-2016.</p><p><strong>Methods:</strong> A retrospective study was done from the year 2013 to 2016. A total of 340 serum samples received in the department of microbiology FAAMCH, were tested for the confirmation of suspected cases of dengue. Dengue NS1 antigen and Dengue IgM antibody ELISA tests were performed for the confirmation of dengue cases. We estimated the incidence by applying age, sex and season adjusted dengue positivity.</p><p><strong>Results:</strong> Out of 340 samples tested, 68 were positive either by NS1 antigen or for IgM antibody ELISA tests. These comprised all age groups of both sexes with higher incidence of cases in young males aged 26– 60 years.</p><p><strong>Conclusion:</strong> From this study, it is apparent that dengue surveillance and control should be enhanced by wider use of laboratory testing to confirm dengue as a cause of fever of unknown origin, especially during the local dengue transmission season.</p>

scholarly journals Standardization of an ELISA test using a recombinant nucleoprotein from the Junin virus as the antigen and serological screening for arenavirus among the population of Nova Xavantina, State of Mato Grosso

2010 ◽  
Vol 43 (3) ◽  
pp. 229-233 ◽  
Author(s):  
Alex Martins Machado ◽  
Glauciane Garcia Figueiredo ◽  
Gelse Maria Campos ◽  
Mario Enrique Lozano ◽  
Aline Rafaela da Silva Rodrigues Machado ◽  
...  
Keyword(s):  
Expression System ◽  
Recombinant Baculovirus ◽  
Hemorrhagic Fever ◽  
Baculovirus Expression System ◽  
Elisa Test ◽  
Serum Samples ◽  
Serological Screening ◽  
Mato Grosso ◽  
Junin Virus ◽  
Junín Virus

INTRODUCTION: Arenavirus hemorrhagic fever is a severe emerging disease. METHODS: Considering that the levels of antibodies against arenavirus in the Brazilian population are completely unknown, we have standardized an ELISA test for detecting IgG antibodies using a recombinant nucleoprotein from the Junin virus as the antigen. This protein was obtained by inserting the gene of the Junin virus nucleoprotein into the genome of Autographa californica nucleopolyhedrovirus, using the Bac-to-Bac baculovirus expression system. This recombinant baculovirus was used to infect S. frugiperda cells (SF9). RESULTS: The infection resulted in synthesis of high concentrations of recombinant protein. This protein was detected on 12.5% polyacrylamide gel and by means of Western blot. Using the standardized ELISA test, 343 samples from the population of Nova Xavantina were analyzed. We observed that 1.4% of the serum samples (five samples) presented antibody titers against arenavirus. CONCLUSIONS: These results show the population studied may present exposure to arenavirus infection.

scholarly journals PREVALENCE OF COXIELLA BURNETII INFECTION IN CATTLE, BLACK BENGAL GOATS AND TICKS IN BANGLADESH

2016 ◽  
Vol 14 (1) ◽  
pp. 65-68 ◽  
Author(s):  
A. Chakrabartty ◽  
P. K. Bhattacharjee ◽  
R. R. Sarker ◽  
A. K. M. A. Rahman ◽  
K. Henning ◽  
...  
Keyword(s):  
Real Time ◽  
Coxiella Burnetii ◽  
Real Time Pcr ◽  
Q Fever ◽  
Elisa Test ◽  
Quantitative Real Time Pcr ◽  
Serum Samples ◽  
Test Kit ◽  
History Of ◽  
Cattle Serum

The objectives of this study were to determine the prevalence of Coxiella burnetii infection in domestic ruminants and to detect Coxiella burnetii DNA from ticks and serum samples. A total of 24 ticks, 91 goats and 81 cattle serum samples with the history of abortion and reproductive disorders were collected from the different areas in Bangladesh. The serum samples were tested by CHEKIT Q-Fever Antibody ELISA Test Kit and Coxiella burnetii DNA was detected by multiplex quantitative real- time PCR. The overall prevalence was 7.6% and 6.1% in goats and cattle, respectively. However, none of seropositive samples and tick samples was positive in quantitative real-time PCR.

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