scholarly journals Clinical, phenotypic and genotypic profile of carbapenem resistant gram negative infections in intensive care units

2021 ◽  
Vol 8 (1) ◽  
pp. 28-34
Author(s):  
Mustafeed Uddin Mohammed ◽  
Manisha D R ◽  
Nagamani K
Keyword(s):  
Intensive Care ◽  
Intensive Care Units ◽  
Gram Negative ◽  
Carbapenem Resistant ◽  
Genotypic Profile

Predicting acquisition of carbapenem-resistant Gram-negative pathogens in intensive care units

2019 ◽  
Vol 103 (2) ◽  
pp. 121-127 ◽  
Author(s):  
L.F. Dantas ◽  
B. Dalmas ◽  
R.M. Andrade ◽  
S. Hamacher ◽  
F.A. Bozza
Keyword(s):  
Intensive Care ◽  
Intensive Care Units ◽  
Gram Negative ◽  
Carbapenem Resistant

scholarly journals Organ donor screening for carbapenem‐resistant gram‐negative bacteria in Italian intensive care units: the DRI n study

2019 ◽  
Vol 20 (1) ◽  
pp. 262-273
Author(s):  
Francesco Procaccio ◽  
Lucia Masiero ◽  
Francesca Vespasiano ◽  
Paolo A. Grossi ◽  
Carlo Gagliotti ◽  
...  
Keyword(s):  
Intensive Care ◽  
Intensive Care Units ◽  
Organ Donor ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Donor Screening ◽  
Carbapenem Resistant

scholarly journals Molecular screening of carbapenemase-producing Gram-negative strains in Romanian intensive care units during a one year survey

2014 ◽  
Vol 63 (10) ◽  
pp. 1303-1310 ◽  
Author(s):  
Irina Gheorghe ◽  
Ilda Czobor ◽  
Mariana Carmen Chifiriuc ◽  
Elvira Borcan ◽  
Camelia Ghiţă ◽  
...  
Keyword(s):  
Intensive Care ◽  
Intensive Care Units ◽  
Rapid Evolution ◽  
Rapid Identification ◽  
Molecular Screening ◽  
Tissue Samples ◽  
Gram Negative ◽  
Carbapenem Resistant ◽  
High Prevalence ◽  
One Year

This is the first study, to our knowledge, performed on a significant number of strains (79 carbapenem-resistant Enterobacteriaceae and 84 carbapenem-resistant non-fermenting Gram-negative rods, GNRs) isolated from tissue samples taken from patients in the intensive care units of two large hospitals in Bucharest, Romania, between 2011 and 2012. The results revealed a high prevalence and great diversity of carbapenemase genes (CRG), in both fermenting and non-fermenting Gram-negative carbapenem-resistant strains. The molecular screening of carbapenem-resistant GNRs revealed the presence of worldwide-distributed CRGs (i.e. bla OXA-48 and bla NDM-1 in Enterobacteriaceae and bla OXA-23, bla VIM-4, bla OXA-10-like, bla OXA-60-like, bla SPM-like and bla GES-like in non-fermenting GNRs), reflecting the rapid evolution and spread of carbapenemase producers, particularly in hospitals. Rapid identification of the colonized or infected patients is required, as are epidemiological investigations to establish the local or imported origin of the respective strains.

scholarly journals Risk Factors for Gastrointestinal Colonization and Acquisition of Carbapenem-Resistant Gram-Negative Bacteria among Patients in Intensive Care Units in Thailand

2018 ◽  
Vol 62 (8) ◽  
Author(s):  
Anong Kiddee ◽  
Kanit Assawatheptawee ◽  
Anamai Na-udom ◽  
Pornpit Treebupachatsakul ◽  
Apirath Wangteeraprasert ◽  
...  
Keyword(s):  
Risk Factors ◽  
Intensive Care ◽  
Intensive Care Units ◽  
Tertiary Care ◽  
Control Measures ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Content Type ◽  
Predominant Species ◽  
Carbapenem Resistant

ABSTRACTThis study was conducted to investigate the prevalence of and risk factors for colonization and acquisition of carbapenem-resistant (CR) Gram-negative bacteria (GNB) among patients admitted to intensive care units (ICUs) in two tertiary care hospitals in northern Thailand. Screening of rectal swab specimens for CR-GNB was performed on patients at ICU admission and discharge. The phenotypes and genotypes of all isolates were determined. Risk factors were analyzed by logistic regression analysis. The overall carriage rate of CR-GNB at admission was 11.6% (32/275), with the most predominant species carried beingAcinetobacter baumannii(n= 15), followed byKlebsiella pneumoniae(n= 9). The risk factor for CR-GNB colonization was hospitalization within the previous 6 months (P= 0.002). During the ICU stay, the rate of CR-GNB acquisition was 25.2% (52/206), with the most predominant species carried beingA. baumannii(n= 28) andK. pneumoniae(n= 13). Risk factors associated with CR-GNB acquisition were the use of an enteral feeding tube (P= 0.008) and administration of third-generation cephalosporins (P= 0.032) and carbapenems (P= 0.045). The most common carbapenemase genes inA. baumanniiandK. pneumoniaewereblaOXA-23/51andblaNDM, respectively. Patient-to-patient transmission was demonstrated in three cases, resulting in the acquisition of CRA. baumannii(2 cases) andK. pneumoniae(1 case) isolates from other patients who were admitted during the same period of time. This is the first Indochinese study screening patients, examining patients for the carriage of CR-GNB, and further demonstrating the transfer of CR-GNB isolates in ICUs. Our study suggests that effective infection control measures are required to limit the spread of CR-GNB within hospitals.

Comparative Study of Phenotypic Detection Methods of Metallo-beta-lactamase (MβL) Among Carbapenem Resistant Gram-Negative Bacilli (GNB) Isolated from Intensive Care Units (IUC) Patients

2020 ◽  
Vol 29 (3) ◽  
pp. 75-80
Author(s):  
Raghda Hager ◽  
Bassant M. Sayed
Keyword(s):  
Intensive Care ◽  
Intensive Care Units ◽  
Carbapenem Resistance ◽  
Detection Methods ◽  
Diffusion Method ◽  
Beta Lactamase ◽  
Gram Negative ◽  
Carbapenem Resistant ◽  
High Prevalence ◽  
Synergy Test

Background: Metallo-beta-lactamase (MβL) mediated resistance is an emergency threat in health care settings, and its identification is essential for treatment and infection control. Objectives: this study aimed to detect the MβL prevalence in Gram negative bacilli (GNB) and to compare its phenotypic detection methods. Methodology: Ninety six (96) isolates of GNB were isolated from different clinical specimens collected from patients admitted to intensive care units (ICU) of Ain Shams Hospitals, from February 2018 to June 2018. Isolates were screened for carbapenem resistance with imipenem 10 µg and meropenem 10 µg discs. The resistant isolates were tested for antibiotic susceptibility by disc diffusion method, and Meropenem minimum inhibitory concentration (MIC) were determinated, then the production of MβL was detected by imipenem-ethylene diamine tetra-acetic acid (EDTA) combined disc test (IPM-EDTA CDT), ceftazidime -EDTA combined disc test (CAZ-EDTA CDT) and Imipenem - EDTA double disc synergy test (IPM-EDTA DDST).Results: Forty three (43) isolates (44.7%) were resistant to carbapenem. Klebsiella pneumoniae (K.pneumoniae) was the most common isolated species; 29 (67.4%) isolates. Forty (40) isolates (93%) were positive for MβL by IPM-EDTA CDT method, whereas 36 (83.7%) were positive by CAZ -EDTA CDT method and 19 isolates (44.2%) were positive for MβL by IPM-EDTA DDST. Conclusion: High prevalence of MβL was detected among our isolates and IMP-EDTA CDT can be used as a phenotypic test in detection of MβL production.

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