scholarly journals Chlorpyrifos removal by Thiobacillus sp. and Clostridium sp. in liquid medium

2018 ◽  
Vol 197 ◽  
pp. 13012
Author(s):  
Thalia Sunaryo ◽  
Hilarion Widyatmoko ◽  
Astri Rinanti
Keyword(s):  
Gas Chromatography ◽  
Liquid Medium ◽  
Temperature Variation ◽  
Mixed Culture ◽  
Removal Efficiency ◽  
Salt Solution ◽  
Contact Time ◽  
Mineral Salt ◽  
Ms Analysis

The bioremediation research of chlorpyrifos has been done to remove the concentration of chlorphyrifos using the natural bacterial Thiobacillus sp. and Clostridium sp.. The efficiency of chlorpyrifos removal was determined by the temperature and contact time. The research was done by adding 100 ppm of chlorpyrifos into the Stone Mineral Salt solution (SMSs) under controlled condition, then each bacterial are added as much as 10% (v/v) with pH 7. To obtain optimum efficiency, this study was conducted with temperature variation (°C) 25, 30, 35, 40 and contact time (hours) 12, 24, 36, 48. Based on Gas Chromatography Mass Spektrofotometer (GC-MS) analysis, the efficiency of removal at temperature (°C) 25, 30, 35, 40 are 43%, 68%, 71% and 52% respectively, while the removal efficiency at contact time (hours) 12, 24, 36, 48 are 43%, 49%, 74%, and 36%. The result showed that 74% removal efficiency by Thiobacillus sp. and Clostridium sp. obtained at 35°C in 36 hours of contact time. Thus, the mixed culture of Thiobacillus sp. and Clostridium sp. are able to synergize for removing the chlorpyrifos at 100 ppm.

scholarly journals Potential of indigenous bacteria to remove cyanide with variations of contact time and temperature

2018 ◽  
Vol 197 ◽  
pp. 13013
Author(s):  
Kezia Eunike ◽  
Astri Rinanti ◽  
Ratnaningsih Ratnaningsih
Keyword(s):  
Gas Chromatography ◽  
Mixed Culture ◽  
Salt Solution ◽  
Contact Time ◽  
Incubation Temperature ◽  
Growth Media ◽  
Initial Condition ◽  
Temperature Variations ◽  
Indigenous Bacteria ◽  
Research Operation

The research on environmental biotechnology has been done in aims to study the ability of mixed culture indigenous bacteria to remove cyanide (KCN-) concentration which is determined by environment conditions of temperature and contact time. The research has been done using the artificial pollutants that contains 500 ppm of KCN- which is exposed to the SMSS (Stone Mineral Salt solution) as a growth media for mixed culture bacteria TBS and CTS. The effect of different environment conditions including temperature and contact time were studied on cyanide removal. Gas Chromatography - Mass Spectrophotometer (GC - MS) method is used to analysed the concentrations of KCN-. Initial condition of research operation is determined to be pH 7 and contact time of 48 hours. The removal of 500 ppm KCN- increases to 71% at incubation temperature 35°C and contact time of 36 hours. This research showed that indigenous bacteria TB1 and CS2 can remove 500 ppm KCN- on all temperature variations between (°C) 25 to 45 and the highest removal occur when contact time is 36 hours.

scholarly journals Removing cyanide by mixed culture at liquid media with variation in pH and cyanide concentration

2018 ◽  
Vol 197 ◽  
pp. 13016
Author(s):  
Corry Valentina ◽  
Rositayanti Hadisoebroto ◽  
Astri Rinanti
Keyword(s):  
Gas Chromatography ◽  
Mixed Culture ◽  
Salt Solution ◽  
Media Analysis ◽  
Contaminated Water ◽  
Liquid Media ◽  
Ph Variation ◽  
Cyanide Concentration ◽  
Preliminary Study ◽  
Cyanide Removal

This preliminary study aims to investigate removal efficiency of cyanide by mixed culture Thiobacillus sp. and Clostridium sp. in a liquid media stone mineral salt solution (SMSs) with controlled condition. This research variation were pH 5, 7, and 9 and cyanide concentrate (ppm) 100, 300, and 500 within erlenmeyer 300 ml, for 48 hours at 30°C. In this research, cyanide artificial (KCN) was presented in liquid media. Analysis of cyanide concentration on liquid medium was done by Gas Chromatography - Mass Spectrometer (GC-MS) method. From the research, it was found that the cyanide removal was 50%, 55%, 53% for cyanide pH variation 5, 7, 9, respectively. In pH 7 was obtained a cyanide removal result of 55% and while for concentration (ppm) 100, 300, 500 each resulted in allowance of 45%, 57% 60%. The higher removal 60% is established at concentration of 500 ppm and pH 7. It could be concluded that mixed culture Thiobacillus sp. and Clostridium sp. could be an alternative to remove cyanide from contaminated water until concentration of 500 ppm with removal percentage of 60%.

Utilisation of Biomass and Hybrid Biochar from Elephant Grass and Low Density Polyethylene for the Competitive Adsorption of Pb(II), Cu(II), Fe(II) and Zn(II) from Aqueous Media

2020 ◽  
Vol 13 ◽  
Author(s):  
Joshua O. Ighalo ◽  
Lois T. Arowoyele ◽  
Samuel Ogunniyi ◽  
Comfort A. Adeyanju ◽  
Folasade M. Oladipo-Emmanuel ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Removal Efficiency ◽  
Contact Time ◽  
Competitive Adsorption ◽  
Adsorption Process ◽  
Aqueous Media ◽  
Polluted Water ◽  
Batch Adsorption ◽  
Order Kinetic ◽  
Elephant Grass

Background: The presence of pollutants in polluted water is not singularized hence pollutant species are constantly in competition for active sites during the adsorption process. A key advantage of competitive adsorption studies is that it informs on the adsorbent performance in real water treatment applications. Objective: This study aims to investigate the competitive adsorption of Pb(II), Cu(II), Fe(II) and Zn(II) using elephant grass (Pennisetum purpureum) biochar and hybrid biochar from LDPE. Method: The produced biochar was characterised by Scanning Electron Microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FTIR). The effect of adsorption parameters, equilibrium isotherm modelling and parametric studies were conducted based on data from the batch adsorption experiments. Results: For both adsorbents, the removal efficiency was >99% over the domain of the entire investigation for dosage and contact time suggesting that they are very efficient for removing multiple heavy metals from aqueous media. It was observed that removal efficiency was optimal at 2 g/l dosage and contact time of 20 minutes for both adsorbent types. The Elovich isotherm and the pseudo-second order kinetic models were best-fit for the competitive adsorption process. Conclusion: The study was able to successfully reveal that biomass biochar from elephant grass and hybrid biochar from LDPE can be used as effective adsorbent material for the removal of heavy metals from aqueous media. This study bears a positive implication for environmental protection and solid waste management.

scholarly journals The Analysis of Bayer Liquor by SPME-GC-MS of Derivatized Organic Poisons

Proceedings ◽  
2021 ◽  
Vol 57 (1) ◽  
pp. 101
Author(s):  
Virgil Badescu ◽  
Raluca Senin
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Bayer Liquor ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Silylating Agent

The aim of this article was the gas chromatography–mass spectrometry (GC-MS) analysis oforganic matter from a residual liquor sample (S.C. Alum S.A., Tulcea), extracted by the solid-phasemicroextraction method (SPMA) and derivatized with N-(tert-butyldimethylsilyl)-Nmethyltrifluoroacetamide(MTBSTFA) as the silylating agent. [...]

scholarly journals Response surface methodology approach for the optimisation of adsorption of hydrolysed polyacrylamide from polymer-flooding wastewater onto steel slag: a good option of waste mitigation

2017 ◽  
Vol 76 (4) ◽  
pp. 776-784 ◽  
Author(s):  
Mijia Zhu ◽  
Jun Yao ◽  
Zhonghai Qin ◽  
Luning Lian ◽  
Chi Zhang
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Removal Efficiency ◽  
Contact Time ◽  
Interactive Effect ◽  
Steel Slag ◽  
Oxygen Demand ◽  
Polymer Flooding ◽  
High Concentration ◽  
Adsorbent Dosage

Wastewater produced from polymer flooding in oil production features high viscosity and chemical oxygen demand because of the residue of high-concentration polymer hydrolysed polyacrylamide (HPAM). In this study, steel slag, a waste from steel manufacturing, was studied as a low-cost adsorbent for HPAM in wastewater. Optimisation of HPAM adsorption by steel slag was performed with a central composite design under response surface methodology (RSM). Results showed that the maximum removal efficiency of 89.31% was obtained at an adsorbent dosage of 105.2 g/L, contact time of 95.4 min and pH of 5.6. These data were strongly correlated with the experimental values of the RSM model. Single and interactive effect analysis showed that HPAM removal efficiency increased with increasing adsorbent dosage and contact time. Efficiency increased when pH was increased from 2.6 to 5.6 and subsequently decreased from 5.6 to 9.3. It was observed that removal efficiency significantly increased (from 0% to 86.1%) at the initial stage (from 0 min to 60 min) and increased gradually after 60 min with an adsorbent dosage of 105.2 g/L, pH of 5.6. The adsorption kinetics was well correlated with the pseudo-second-order equation. Removal of HPAM from the studied water samples indicated that steel slag can be utilised for the pre-treatment of polymer-flooding wastewater.

scholarly journals Development and validation of the method for the detection of glimepiride via derivatization employing N-methyl-N-(trimethylsilyl) trifluoroacetamide using gas chromatography-mass spectrometry

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Priyanka Verma ◽  
Atul Bajaj ◽  
R. M. Tripathi ◽  
Sudhir K. Shukla ◽  
Suman Nagpal
Keyword(s):  
Diabetes Mellitus ◽  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Drug Users ◽  
Coefficient Of Determination ◽  
Gas Chromatography Mass Spectrometry ◽  
Biological Matrices ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
The Stability

Abstract Background Recent advances in the diversified anti-diabetic drugs have appeared in the startling increase in the count of poisoning cases. The epidemics of diabetes mellitus are increasing; hence, the no. of anti-diabetic drug users raised by 42.9%. The use of glimepiride raised to 24%. As the toxicity and drug cases are also escalating with increasing epidemics of diabetes mellitus, a novel gas chromatography-mass spectrometry (GC-MS) method for detecting glimepiride in biological matrices is developed. Results Liquid-liquid extraction method was employed by using 1-butanol: hexane (50:50, v/v) under an alkaline medium, and then back extraction was done via acetic acid. Distinct derivatization techniques were employed for the sample preparation for GC-MS analysis, i.e., silylation and acylation. Derivatization approaches were optimized under different parameters, i.e., reaction temperature and reaction time. N-Methyl-N-(trimethylsilyl) trifluoroacetamide [MSTFA] was found to be the best sound derivatization reagent for the GC-MS analysis of glimepiride. Total ion current (TIC) mode was selected for the monitoring of ions of trimethylsilyl (TMS) derivative of glimepiride with an m/z ratio of 256. Distinct parameters like specificity, carryover, stability, precision, and accuracy were evaluated for validating the identification method. The GC-MS method is found to be linear and illustrated within the range 500 to 2500 ng/ml with the value of R2 (coefficient of determination) at 0.9924. The stability of the extracted and derivatized glimepiride was accessed with regard to processed/extracted sample conditions and autosampler conditions, respectively. Accuracy at each concentration level was within the + 15% of the nominal concentration. Precision (%) for the interday and intraday analysis was found to be in the respectable spectrum. Conclusion Henceforth, the proposed GC-MS method can be employed for the determination of glimepiride in biological matrices.

scholarly journals From Waste to Biosorbent: Removal of Congo Red from Water by Waste Wood Biomass

Water ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 279 ◽  
Author(s):  
Marija Stjepanović ◽  
Natalija Velić ◽  
Antonela Galić ◽  
Indira Kosović ◽  
Tamara Jakovljević ◽  
...  
Keyword(s):  
Removal Efficiency ◽  
Congo Red ◽  
Contact Time ◽  
Synthetic Wastewater ◽  
Wood Biomass ◽  
Waste Wood ◽  
Biosorption Capacity ◽  
Biosorption Process ◽  
Cr Concentration ◽  
Cr Removal

The aim of the study was to screen the waste wood biomass of 10 wood species as biosorbents for synthetic dye Congo Red (CR) removal from water and to single out the most efficient species for further batch biosorption experiments. Euroamerican poplar (EP), the most efficient species achieving 71.8% CR removal and biosorption capacity of 3.3 mg g−1, was characterized by field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy (FTIR). Different factors affecting the biosorption process were investigated: initial biosorbent concentration (1–10 g dm−3), contact time (5–360 min), initial CR concentration (10–100 mg dm−3), and the initial pH (pH = 4–9). The results showed that CR removal efficiency increased with the increase of biosorbent concentration and contact time. Increase of initial CR concentration led to an increase of the biosorption capacity, but also a decrease of CR removal efficiency. The highest CR removal efficiency was achieved at pH = 4, while at pH = 9 a significant decrease was noticed. The percentage of CR removal from synthetic wastewater was 18.6% higher than from model CR solution. The Langmuir model fitted well the biosorption data, with the maximum biosorption capacity of 8 mg g−1. The kinetics data were found to conform to the pseudo-second-order kinetics model.

scholarly journals PHYTOCHEMICAL EVALUATION OF TILIACORA RACEMOSA COLEBR. USING GAS CHROMATOGRAPHY - MASS SPECTROMETRY (GC-MS)

2018 ◽  
Vol 11 (2) ◽  
pp. 350
Author(s):  
Yogeshwari C ◽  
Kumudha P
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Bioactive Compounds ◽  
Quinic Acid ◽  
Physicochemical Analysis ◽  
Drug Formulation ◽  
Gas Chromatography Mass Spectrometry ◽  
Isolation And Characterization ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis

 Objective:The objective of this study is to characterize the phytoconstituents of Tiliacora racemosa Colebr. using gas chromatography mass spectrometry (GC-MS).Methods: Preliminary phytochemical and physicochemical analysis was carried out using standard procedures. GC-MS analysis of methanolic extract was carried out using Thermo GC-Trace Ultra version: 5.0, Thermo MS DSQ with a DB 35MS capillary standard non-polar column and gas chromatograph interfaced to a mass selective detector (MS DSQ II) with Xcalibur software.Results: Preliminary phytochemical screening revealed the presence of alkaloids, flavonoids, phenols, tannins, triterpenoids, steroids, proteins and amino acids, carbohydrates, saponins and coumarin. Quinones, anthraquinones, glycosides and fixed oil were absent. GC-MS analysis revealed the presence of 28 compounds of which quinic acid (retention times [RT]: 15.65) and inositol, 1-deoxy-(CAS) (RT: 19.24) was observed as abundant compounds.Conclusion: The presence of various bioactive compounds confirms the medicinal importance and it’s application for curing various diseases by traditional practitioners. However, isolation and characterization of potential bioactive compounds would lead to drug formulation.

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