scholarly journals Comparative proteomic analysis of Bombyx mori hemolymph and fat body after calorie restriction.

2010 ◽  
Vol 57 (4) ◽  
Author(s):  
Huiqing Chen ◽  
Yijia Li ◽  
Keping Chen ◽  
Qin Yao ◽  
Guohui Li ◽  
...  
Keyword(s):  
Life Span ◽  
Bombyx Mori ◽  
Calorie Restriction ◽  
Growth And Development ◽  
Fat Body ◽  
Silkworm Larvae ◽  
Proteomic Approach ◽  
Extend Life Span ◽  
Regulation Of Growth ◽  
Insight Into

Calorie restriction (CR) is known to extend life span from yeast to mammals. To gain an insight into the effects of CR on growth and development of the silkworm Bombyx mori at protein level, we employed comparative proteomic approach to investigate proteomic differences of hemolymph and fat body of the silkworm larvae subjected to CR. Thirty-nine differentially expressed proteins were identified by MALDI TOF/TOF MS. Among them, 19 were from the hemolymph and 20 from the fat body. The hemolymph of the CR group contained two down-regulated and 17 up-regulated proteins, whereas the fat body contained 15 down-regulated and five up-regulated ones. These proteins belonged to those functioning in immune system, in signal transduction and apoptosis, in regulation of growth and development, and in energy metabolism. Our results suggest that CR can alter the expression of proteins related to the above four aspects, implying that these proteins may regulate life span of the silkworm through CR.

scholarly journals Chromatin-modifying genetic interventions suppress age-associated transposable element activation and extend life span in Drosophila

2016 ◽  
Vol 113 (40) ◽  
pp. 11277-11282 ◽  
Author(s):  
Jason G. Wood ◽  
Brian C. Jones ◽  
Nan Jiang ◽  
Chengyi Chang ◽  
Suzanne Hosier ◽  
...  
Keyword(s):  
Life Span ◽  
Dietary Restriction ◽  
Fat Body ◽  
Transcriptase Inhibitor ◽  
Dna Double Strand Breaks ◽  
Expression Of Genes ◽  
Age Related ◽  
Extend Life Span ◽  
Genetic Interventions ◽  
Heterochromatin Structure

Transposable elements (TEs) are mobile genetic elements, highly enriched in heterochromatin, that constitute a large percentage of the DNA content of eukaryotic genomes. Aging in Drosophila melanogaster is characterized by loss of repressive heterochromatin structure and loss of silencing of reporter genes in constitutive heterochromatin regions. Using next-generation sequencing, we found that transcripts of many genes native to heterochromatic regions and TEs increased with age in fly heads and fat bodies. A dietary restriction regimen, known to extend life span, repressed the age-related increased expression of genes located in heterochromatin, as well as TEs. We also observed a corresponding age-associated increase in TE transposition in fly fat body cells that was delayed by dietary restriction. Furthermore, we found that manipulating genes known to affect heterochromatin structure, including overexpression of Sir2, Su(var)3–9, and Dicer-2, as well as decreased expression of Adar, mitigated age-related increases in expression of TEs. Increasing expression of either Su(var)3–9 or Dicer-2 also led to an increase in life span. Mutation of Dicer-2 led to an increase in DNA double-strand breaks. Treatment with the reverse transcriptase inhibitor 3TC resulted in decreased TE transposition as well as increased life span in TE-sensitized Dicer-2 mutants. Together, these data support the retrotransposon theory of aging, which hypothesizes that epigenetically silenced TEs become deleteriously activated as cellular defense and surveillance mechanisms break down with age. Furthermore, interventions that maintain repressive heterochromatin and preserve TE silencing may prove key to preventing damage caused by TE activation and extending healthy life span.

scholarly journals Long-lived Indy and calorie restriction interact to extend life span

2009 ◽  
Vol 106 (23) ◽  
pp. 9262-9267 ◽  
Author(s):  
P.-Y. Wang ◽  
N. Neretti ◽  
R. Whitaker ◽  
S. Hosier ◽  
C. Chang ◽  
...  
Keyword(s):  
Life Span ◽  
Calorie Restriction ◽  
Extend Life Span

scholarly journals Calorie Restriction: Is AMPK a Key Sensor and Effector?

Physiology ◽  
2011 ◽  
Vol 26 (4) ◽  
pp. 214-224 ◽  
Author(s):  
Carles Cantó ◽  
Johan Auwerx
Keyword(s):  
Protein Kinase ◽  
Life Span ◽  
Calorie Restriction ◽  
Energy Availability ◽  
Beneficial Effects ◽  
Extend Life Span ◽  
Energy Sensor ◽  
External Cues ◽  
Amp Activated Protein Kinase

Dietary restriction can extend life span in most organisms tested to date, suggesting that mechanisms sensing nutrient and energy availability might regulate longevity. The AMP-activated protein kinase (AMPK) has emerged as a key energy sensor with the ability to transcriptionally reprogram the cell and metabolically adapt to external cues. In this review, we will discuss the possible role of AMPK in the beneficial effects of calorie restriction on health and life span.

scholarly journals Dietary Interventions to Extend Life Span and Health Span Based on Calorie Restriction

2010 ◽  
Vol 65A (7) ◽  
pp. 695-703 ◽  
Author(s):  
R. K. Minor ◽  
J. S. Allard ◽  
C. M. Younts ◽  
T. M. Ward ◽  
R. de Cabo
Keyword(s):  
Life Span ◽  
Calorie Restriction ◽  
Dietary Interventions ◽  
Health Span ◽  
Extend Life Span

The effect of calorie restriction on growth and development in silkworm,Bombyx mori

2009 ◽  
Vol 71 (3) ◽  
pp. 159-172 ◽  
Author(s):  
Yijia Li ◽  
Keping Chen ◽  
Qin Yao ◽  
Jun Li ◽  
Yong Wang ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Calorie Restriction ◽  
Growth And Development ◽  
Silkworm Bombyx Mori

BmNPV alters NADP-dependent malate dehydrogenase activity and associated macromolecules and retards growth and development of the mulberry silkworm, Bombyx mori L., during the final instar

2002 ◽  
Vol 80 (8) ◽  
pp. 1451-1459 ◽  
Author(s):  
Natraj Krishnan ◽  
Anathbandhu Chaudhuri
Keyword(s):  
Bombyx Mori ◽  
Malate Dehydrogenase ◽  
Growth And Development ◽  
Fat Body ◽  
Post Inoculation ◽  
Body Tissues ◽  
Baculovirus Infection ◽  
Bombyx Mori L ◽  
Mulberry Silkworm ◽  
Silkworm Bombyx Mori

Changes in tissue-specific NADP-dependent malate dehydrogenase (MDH) activity and protein and cholesterol contents and retardation of growth and development in the mulberry silkworm, Bombyx mori L., induced by infection with a baculovirus, the B. mori nucleopolyhedrovirus (BmNPV), were investigated. The study revealed that the relative growth rate and development of the fifth-instar larva was significantly inhibited during the progression of nuclear polyhedrosis disease at various times post inoculation (p.i.). Percent pupation and reproductive output were also adversely affected. NADP-dependent MDH activity in the hemolymph peaked sharply at 6 h p.i. and then gradually decreased, reaching a minimum at 264 h p.i., with exceptions at 30 and 72 h p.i. These levels of MDH activity were different from those in non-infected controls. In fat-body tissues, NADP-dependent MDH activity was significantly higher in infected insects than in non-infected controls of the same age. These results indicate that baculovirus infection causes significant changes in intermediary metabolic pathways, causing a significant fall and rise in protein and cholesterol contents in tissues during development of fifth-instar larvae.

Identification and characterization of novel short-type BmPGRP-S4 from the silkworm, Bombyx mori, involved in innate immunity

2020 ◽  
Vol 75 (1-2) ◽  
pp. 13-21 ◽  
Author(s):  
Qiang Wang ◽  
Meijia Ren ◽  
Xiaoyong Liu ◽  
Hengchuan Xia ◽  
Keping Chen
Keyword(s):  
Immune Response ◽  
Bombyx Mori ◽  
Innate Immune Response ◽  
Fat Body ◽  
Innate Immune ◽  
Luciferase Reporter ◽  
Sequence Length ◽  
Silkworm Larvae ◽  
Gene Assay ◽  
Short Type

AbstractPeptidoglycan recognition proteins (PGRPs) are pattern recognition receptors that can recognize bacterial peptidoglycans and trigger the innate immune response of insects. Here, we identified and characterized a novel short-type Bombyx mori peptidoglycan recognition proteins short-4 (BmPGRP-S4) in a lepidopteran insect, Bombyx mori. BmPGRP-S4 exhibited a cDNA sequence length of 600 bp, encoding 199 aa with a protein molecular weight of 22 kDa. Multiple sequence alignment revealed that BmPGRP-S4 contains a conserved PGRP domain. Quantitative real-time polymerase chain reaction analysis showed that BmPGRP-S4 is highly expressed in the early developmental stages of silkworm larvae and presents tissue-specific expression in hemocytes. Interestingly, BmPGRP-S4 expression is significantly induced by bacterial infection in the midgut, fat body, and hemocytes. Furthermore, a dual luciferase reporter gene assay revealed that BmPGRP-S4 can activate the expression of the antimicrobial peptide genes lebocin, moricin, cecropin D, cecropin B, and attacin. Taken together, these results suggest that BmPGRP-S4 plays an important role in the innate immune response of silkworms.

Positive growth and development: From distress to coping across the life-span

2010 ◽  
Author(s):  
Erica Frydenberg ◽  
Krys Kaniasty ◽  
Erica Frydenberg ◽  
Barbara Jones ◽  
Esther Greenglass ◽  
...  
Keyword(s):  
Life Span ◽  
Growth And Development ◽  
Positive Growth

scholarly journals Transcriptome analysis reveals potential function of long non-coding RNAs in 20-hydroxyecdysone regulated autophagy in Bombyx mori

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Huili Qiao ◽  
Jingya Wang ◽  
Yuanzhuo Wang ◽  
Juanjuan Yang ◽  
Bofan Wei ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Target Gene ◽  
Fat Body ◽  
Expression Profiles ◽  
Functional Characterization ◽  
Differentially Expressed ◽  
Post Injection ◽  
Biological Processes ◽  
Potential Target ◽  
Non Coding Rnas

Abstract Background 20-hydroxyecdysone (20E) plays important roles in insect molting and metamorphosis. 20E-induced autophagy has been detected during the larval–pupal transition in different insects. In Bombyx mori, autophagy is induced by 20E in the larval fat body. Long non-coding RNAs (lncRNAs) function in various biological processes in many organisms, including insects. Many lncRNAs have been reported to be potential for autophagy occurrence in mammals, but it has not been investigated in insects. Results RNA libraries from the fat body of B. mori dissected at 2 and 6 h post-injection with 20E were constructed and sequenced, and comprehensive analysis of lncRNAs and mRNAs was performed. A total of 1035 lncRNAs were identified, including 905 lincRNAs and 130 antisense lncRNAs. Compared with mRNAs, lncRNAs had longer transcript length and fewer exons. 132 lncRNAs were found differentially expressed at 2 h post injection, compared with 64 lncRNAs at 6 h post injection. Thirty differentially expressed lncRNAs were common at 2 and 6 h post-injection, and were hypothesized to be associated with the 20E response. Target gene analysis predicted 6493 lncRNA-mRNA cis pairs and 42,797 lncRNA-mRNA trans pairs. The expression profiles of LNC_000560 were highly consistent with its potential target genes, Atg4B, and RNAi of LNC_000560 significantly decreased the expression of LNC_000560 and Atg4B. These results indicated that LNC_000560 was potentially involved in the 20E-induced autophagy of the fat body by regulating Atg4B. Conclusions This study provides the genome-wide identification and functional characterization of lncRNAs associated with 20E-induced autophagy in the fat body of B. mori. LNC_000560 and its potential target gene were identified to be related to 20-regulated autophagy in B. mori. These results will be helpful for further studying the regulatory mechanisms of lncRNAs in autophagy and other biological processes in this insect model.

Sign in / Sign up

Export Citation Format

Share Document