Interactions in Entomology: Enhanced Infectivity of Entomopathogenic Viruses by Fluorescent Brighteners
Fluorescent brighteners or optical brighteners, chemicals that absorb energy from ultraviolet light (UV) and emit it as visible light, have been studied as UV protectants for entomopathogens to extend their effectiveness in the field as biocontrol agents. Some stilbene fluorescent brighteners have been demonstrated both to provide some protection from UV degradation and to enhance infectivity independently of the UV protection for several entomopathogenic viruses. Although the mode of action of this enhanced infectivity is not fully understood, enhanced infectivity of 10 nucleopolyhedroviruses and a granulovirus (Baculoviridae), a cypovirus (Reoviridae), an iridovirus (Iridoviridae), and an entomopoxvirus (Poxviridae) has been demonstrated. The enhanced infectivity produced by the fluorescent brighteners generally resulted in earlier mortality and in some cases extended infectivity of the virus to older instars. In a few cases the host range was extended, i.e., viruses which were not infective for a particular species under normal conditions were infective with the addition of a fluorescent brightener. Thus, if proper formulations can be made, the fluorescent brighteners have the potential to increase the effectiveness of certain entomopathogenic viruses for biocontrol of certain lepidopterous pests of forest and agricultural crops.