β-sitosterol Mediated Silver Nanoparticles Induce Cytotoxicity in Human Colon Cancer HT-29 Cells

Background: Silver nanoparticles (AgNP) are commonly used metallic nanoparticles in health care systems. Colon cancer incidence is increasing worldwide. In this study, AgNP was synthesized using β-sitosterol and its cytotoxic potential was evaluated in human colon cancer (HT-29) cells. Methods: Characterization of AgNP was analyzed by TEM and spectrophotometry analysis. HT-29 cells were treated with different concentrations (2, 4, 6, 8 and 10 ng/ml) of AgNPs and cytotoxicity was evaluated by MTT assay. The apoptosis was analyzed by the flow cytometry. The expression of p53 protein was analyzed by western blotting. Results: β-sitosterol mediated AgNP are spherical in shape and induced concentration-dependent cytotoxicity in HT-29 cells. AgNP caused apoptosis related morphological changes as evidenced by annexin positive staining. AgNP treatments also induced the p53 expression in HT-29 cells. Conclusion: Our present result suggests that β-sitosterol mediated AgNP induce apoptosis in colon cancer cells and this finding may pave the way for further experimental analysis in vivo.

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Antineoplastic Effect of PAC Capped Silver Nanoparticles Promote Apoptosis in HT-29 Human Colon Cancer Cells

Journal of Cluster Science ◽

10.1007/s10876-019-01510-1 ◽

2019 ◽

Vol 30 (2) ◽

pp. 483-493 ◽

Cited By ~ 3

Author(s):

Suganya Mani ◽

Mythili Gnanamangai Balasubramanian ◽

Ponmurugan Ponnusamy ◽

Paranthaman Vijayan

Keyword(s):

Colon Cancer ◽

Silver Nanoparticles ◽

Cancer Cells ◽

Human Colon ◽

Colon Cancer Cells ◽

Human Colon Cancer ◽

Antineoplastic Effect ◽

Human Colon Cancer Cells ◽

Ht 29

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Biogenic Synthesis, Characterization and Evaluation of Silver Nanoparticles from Aspergillus niger JX556221 Against Human Colon Cancer Cell Line HT-29

Journal of Nanoscience and Nanotechnology ◽

10.1166/jnn.2018.15364 ◽

2018 ◽

Vol 18 (5) ◽

pp. 3673-3681 ◽

Cited By ~ 10

Author(s):

Wang Chengzheng ◽

Wen Jiazhi ◽

Chen Shuangjiang ◽

Mallappa Kumara Swamy ◽

Uma Rani Sinniah ◽

...

Keyword(s):

Colon Cancer ◽

Silver Nanoparticles ◽

Cancer Cell Line ◽

Human Colon ◽

Colon Cancer Cell Line ◽

Colon Cancer Cell ◽

Human Colon Cancer Cell ◽

Biogenic Synthesis ◽

Human Colon Cancer ◽

Ht 29

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Cytotoxic potentials of silibinin assisted silver nanoparticles on human colorectal HT-29 cancer cells

Bioinformation ◽

10.6026/97320630016817 ◽

2020 ◽

Vol 16 (11) ◽

pp. 817-827

Author(s):

Kiren Jackson ◽

Keyword(s):

Silver Nanoparticles ◽

Cancer Cells ◽

Morphological Changes ◽

P53 Protein ◽

P53 Expression ◽

P53 Protein Expression ◽

Transmission Electron ◽

Colorectal Cancer Cells ◽

Ht 29 ◽

Apoptotic Change

It is of interest to study the cytotoxicity of silibinin assisted silver nanoparticles in human colorectal (HT-29) cancer cells. Silver nanoparticles were synthesized using silibinin as a reducing agent. The synthesized silibinin assisted silver nanoparticles (SSNPs) were characterized and analyzed using a transmission electron microscope and spectrophotometer. The SSNPs synthesized in this study are spherical and their size ranges from 10 to 80 nm. HT-29 cells were treated with different concentrations (2, 4, 6, 8 and 10 ng/mL) of SSNPs and cytotoxicity was evaluated. The apoptosis was using flow cytometry. p53 protein expression using western blot. SSNPs are induced a decrease in viability and increased concentration-dependent cytotoxicity in HT-29 cells. SSNPs treatment also caused apoptosis-related morphological changes. SSNPs treatments at 8 and 16 ng/ml showed a prominent apoptotic change i.e., 70.3% and 83.6% respectively, and decreased viability of HT-29 cells 20% and 11.2% respectively as compared to control cells. SSNPs treatments induced p53 expression in HT-29 cells. Data shows that SSNPs have the potential to induce apoptosis in colorectal cancer cells. This provides insights for the further evaluation of SSNPs in fighting colon cancer.

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