Assessment of apoptosis and appearance of hepatocyte growth factor in placenta at different gestational ages: A cross-sectional study

Background: Fetal growth is determined by the interaction between mother and fetus using the placental interface throughout the pregnancy. Objective: To research apoptosis and appearance of hepatocyte growth factor (HGF) in placentas of different gestational ages and to describe the anthropometrical and clinical indices of mothers and newborns. Materials and Methods: The study material was obtained from 53 human immunodeficiency virus negative pregnant women of legal age without systemic diseases. The staining of placental apoptotic cells was processed by a standard in situ cell death detection kit. The detection of HGF was provided by the ImmunoCruz goat ABC Staining System protocol sc-2023. Relative distribution of positive structures was evaluated using the semiquantitative counting method. Results: The mean rank value of the amount of HGF-containing cells (cytotrophoblasts, syncytiotrophoblasts, extravillous trophoblasts, Höfbauer cells, and cells of extraembryonic mesoderm) was 1.61 ± 0.94. Apoptotic cells (cytotrophoblasts, syncytiotrophoblasts, extravillous trophoblasts, and cells of extraembryonic mesoderm) were found in all placental samples of various gestational ages (term 13.00 ± 13.05 and preterm 27.00 ± 18.25); in general, their amount decreased with advancing gestational age of the placenta (p < 0.01). Conclusion: Weight of a placenta directly depends on the gestational age and correlates with the main fetal anthropometrical parameters (weight, length, and head and chest circumferences). The decrease in HGF-containing and apoptotic cells with advancing gestation depends on the adaptation potential of the placenta, proving the other ways of cellular disposition. Key words: Pregnancy, Placenta, Gestational age, Apoptosis, Immunohistochemistry.

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Association of hemoglobin concentration with handgrip strength in relation to hepatocyte growth factor levels among elderly Japanese men aged 60–69 years: a cross-sectional study

Environmental Health and Preventive Medicine ◽

10.1186/s12199-018-0744-x ◽

2018 ◽

Vol 23 (1) ◽

Author(s):

Yuji Shimizu ◽

Hirotomo Yamanashi ◽

Yuko Noguchi ◽

Jun Koyamatsu ◽

Mako Nagayoshi ◽

...

Keyword(s):

Growth Factor ◽

Hepatocyte Growth Factor ◽

Handgrip Strength ◽

Hemoglobin Concentration ◽

Cross Sectional Study ◽

Sectional Study ◽

Cross Sectional ◽

Japanese Men ◽

Elderly Japanese ◽

Hepatocyte Growth

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Favorable Cardiovascular Health Is Associated With Lower Hepatocyte Growth Factor Levels in the Multi-Ethnic Study of Atherosclerosis

Frontiers in Cardiovascular Medicine ◽

10.3389/fcvm.2021.760281 ◽

2022 ◽

Vol 8 ◽

Author(s):

Olatokunbo Osibogun ◽

Oluseye Ogunmoroti ◽

Richard A. Ferraro ◽

Chiadi E. Ndumele ◽

Gregory L. Burke ◽

...

Keyword(s):

Growth Factor ◽

Hepatocyte Growth Factor ◽

Cardiovascular Health ◽

Sociodemographic Factors ◽

Endothelial Injury ◽

Cvd Risk ◽

Cross Sectional ◽

Ideal Metrics ◽

Potential Biomarker ◽

Hepatocyte Growth

Introduction: Hepatocyte growth factor (HGF) is a cytokine released in response to endothelial injury and a potential biomarker of cardiovascular disease (CVD) risk. We examined the association between cardiovascular health (CVH) and HGF in a multi-ethnic cohort of adults free from CVD at baseline.Methods: This cross-sectional study conducted between 2020 and 2021 used MESA baseline examination data (2000–2002) from 6,490 US adults aged 45–84 years. The independent variable was CVH measured by the CVH score and number of ideal metrics. The score was derived from seven metrics: smoking, body mass index, physical activity, diet, total cholesterol, blood pressure and blood glucose. Each metric was scored 0 points (poor), 1 point (intermediate) and 2 points (ideal). The total CVH score ranged from 0 to 14. An inadequate score was 0–8, average, 9–10 and optimal, 11–14. The dependent variable was logarithmically transformed HGF. We used regression analyses to estimate associations between CVH and HGF adjusting for sociodemographic factors.Results: Participants' mean (SD) age was 62 (10) years. Fifty-three percent were female. A one-unit increment in the CVH score was significantly associated with 3% lower HGF levels. Average and optimal CVH scores were significantly associated with 8% and 12% lower HGF levels, respectively, compared to inadequate scores. Additionally, a greater number of ideal metrics was associated with lower HGF levels.Conclusion: Favorable CVH was significantly associated with lower HGF levels in this ethnically diverse cohort. Interventions aimed at promoting and preserving favorable CVH may reduce the risk of endothelial injury as indicated by lower serum HGF levels.

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Changes in Maternal Serum Transforming Growth Factor Beta-1 during Pregnancy: A Cross-Sectional Study

BioMed Research International ◽

10.1155/2013/318464 ◽

2013 ◽

Vol 2013 ◽

pp. 1-5 ◽

Cited By ~ 10

Author(s):

Mandeep Singh ◽

Ngozi C. Orazulike ◽

Jill Ashmore ◽

Justin C. Konje

Keyword(s):

Growth Factor ◽

Gestational Age ◽

Transforming Growth Factor Beta ◽

Transforming Growth Factor ◽

Cross Sectional Study ◽

Maternal Serum ◽

Cross Sectional ◽

Appropriate For Gestational Age ◽

Growth Factor Beta ◽

Circulating Levels

Changes in circulating levels of maternal serum transforming growth factor beta-1 (TGF-β1), collected from 98 women (AGA) at different gestational ages (10–38 weeks) were measured and comparisons were made between levels in pregnant and nonpregnant controls and also between 10 women with small-for-gestational age (SGA) and 7 with appropriate-for-gestational age (AGA) fetuses. Maternal serum TGF-β1 levels at all stages of pregnancy were higher than those in normal healthy nonpregnant adults. The mean TGF-β1 levels in SGA pregnancies at 34-week gestation (32.5 + 3.2 ng/mL) were significantly less than those in AGA pregnancies (39.2 + 9.8 ng/mL) while at 38-week gestation, the levels were similar in the two groups (36.04 + 4.3 versus 36.7 + 7.0 ng/mL). This differential change in TGF-β1 levels is probably an important modulating factor in the aetiopathogenesis of abnormal intrauterine fetal growth.

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