scholarly journals DOES Beta vulgaris L. HAVE LCYB GENE?

2015 ◽  
Vol 2 (1) ◽  
pp. 92
Author(s):  
Yulius Dedy Irawan ◽  
Ilvi Chamalia ◽  
Dahlia , ◽  
Dwi Listyorini
Keyword(s):  
Arabidopsis Thaliana ◽  
Phylogenetic Relationship ◽  
Beta Vulgaris ◽  
Beta Carotene ◽  
Taraxacum Officinale ◽  
Pigment Synthesis ◽  
Plant Model ◽  
Blast Analysis ◽  
Lilium Lancifolium ◽  
Forward Primer

Beta carotene is a pigment that generally shared by all plants. This pigment synthesis is catalized by lycopene beta cyclase enzime. This enzyme is encoded by the LCYB gene. This research aimed to obtain the sequence of LCYB gene isolated from beet (Beta vulgaris L.) leaves. Several experiments have been done to isolate the LCYB gene. In the first experiment, the gene primers are designed using Pick Primer software based on conserved region of Taraxacum officinale (accession no.AB247456.1) and Lilium lancifolium LCYB (accession no. GU471230.1).The forward primer is (5'-TGTCGTGGTGGATCTTGTGG-3') and the reverse is (5'-ACACCTGTTGAGCGACAGAC-3').At the first experiment, the gene target’s sequence was not obtained as the phylogenetic relationship between Beta vulgaris, Taraxacum officinale, and Lilium lancifolium were too far.In the second experiment, the primers are designed using LCYB gene in Arabidopsis thaliana (accsession no.NM_111858.2), with considering Arabidopsis thaliana as a plant model. The primers are (5’­TGGGACAGCAGGAATGGTTC-3’) as the forward primer and (5’-GAGAAGAGCGACAACCCGAA-3’) the reverse. By using BLAST analysis in the second experiment, it suggests that the fragment amplified by forward primer has similarity with trnL gene.This study seems to amplify the trnL gene instead of LCYB gene. This facts leads to bigger question whether or not the Beta vulgaris L has the LCYB gene.Keywords : Beta carotene, Lycopene Beta Cyclase, Beta vulgaris L, LCYB gene

scholarly journals Mapping-based genome size estimation

2019 ◽  
Author(s):  
Boas Pucker
Keyword(s):  
Arabidopsis Thaliana ◽  
Zea Mays ◽  
Vitis Vinifera ◽  
Genome Size ◽  
Solanum Lycopersicum ◽  
Beta Vulgaris ◽  
Brachypodium Distachyon ◽  
Size Estimation ◽  
Short Read ◽  
Alternative Approach

AbstractWhile the size of chromosomes can be measured under a microscope, the size of genomes cannot be measured precisely. Biochemical methods and k-mer distribution-based approaches allow only estimations. An alternative approach to predict the genome size based on high contiguity assemblies and short read mappings is presented here and optimized onArabidopsis thalianaandBeta vulgaris.Brachypodium distachyon,Solanum lycopersicum,Vitis vinifera, andZea mayswere also analyzed to demonstrate the broad applicability of this approach. Mapping-based Genome Size Estimation (MGSE) and additional scripts are available on github:https://github.com/bpucker/MGSE.

Growth and persistence of Listeria monocytogenes isolates on the plant model Arabidopsis thaliana

2008 ◽  
Vol 25 (5) ◽  
pp. 698-704 ◽  
Author(s):  
Sara R. Milillo ◽  
Jasmine M. Badamo ◽  
Kathryn J. Boor ◽  
Martin Wiedmann
Keyword(s):  
Arabidopsis Thaliana ◽  
Listeria Monocytogenes ◽  
Plant Model

Comparison of the toxicity of silver nanoparticles and silver ions on the growth of terrestrial plant model Arabidopsis thaliana

2013 ◽  
Vol 25 (9) ◽  
pp. 1947-1956 ◽  
Author(s):  
Haifeng Qian ◽  
Xiaofeng Peng ◽  
Xiao Han ◽  
Jie Ren ◽  
Liwei Sun ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Arabidopsis Thaliana ◽  
Silver Ions ◽  
Plant Model ◽  
Terrestrial Plant

scholarly journals NATURAL DYES USED IN TINCTORIAL PRACTICE

1970 ◽  
Vol 63 ◽  
Author(s):  
Crinela Dumitrescu ◽  
Laura Monica Gorghiu ◽  
R. L. Olteanu ◽  
M. Bumbac
Keyword(s):  
Beta Vulgaris ◽  
Hypericum Perforatum ◽  
Current Knowledge ◽  
Taraxacum Officinale ◽  
Natural Dyes ◽  
Origanum Vulgare ◽  
Sambucus Nigra ◽  
Rubia Tinctorum ◽  
Wool Fibers ◽  
Brassica Oleraceae

The utilization of natural dyes became more important in the last years. On this basis we propose to present a series of ways to paint in the form of “modernized” recipe on the basis of current knowledge regarding the fixation process. It was achieved dying of wool fibers with dyes extracted from Hypericum perforatum, Origanum vulgare, Rubia tinctorum, Quercus pedunculata, Fraxinus excelsior, Sambucus nigra, Allium cepa, Taraxacum officinale, Vaccinium myrtillus, Beta vulgaris, Brassica oleraceae, Hyancinthus orientalis.

scholarly journals Comparative genomic analysis of the PKS genes in five species and expression analysis in upland cotton

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3974 ◽  
Author(s):  
Xueqiang Su ◽  
Xu Sun ◽  
Xi Cheng ◽  
Yanan Wang ◽  
Muhammad Abdullah ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Gene Family ◽  
Cotton Fiber ◽  
Upland Cotton ◽  
Polyketide Synthase ◽  
Genomic Analysis ◽  
Pcr Analysis ◽  
Comparative Genomic ◽  
Type Iii Polyketide Synthase ◽  
Pigment Synthesis

Plant type III polyketide synthase (PKS) can catalyse the formation of a series of secondary metabolites with different structures and different biological functions; the enzyme plays an important role in plant growth, development and resistance to stress. At present, the PKS gene has been identified and studied in a variety of plants. Here, we identified 11 PKS genes from upland cotton (Gossypium hirsutum) and compared them with 41 PKS genes inPopulus tremula,Vitis vinifera,Malus domesticaandArabidopsis thaliana. According to the phylogenetic tree, a total of 52 PKS genes can be divided into four subfamilies (I–IV). The analysis of gene structures and conserved motifs revealed that most of the PKS genes were composed of two exons and one intron and there are two characteristic conserved domains (Chal_sti_synt_N and Chal_sti_synt_C) of the PKS gene family. In our study of the five species, gene duplication was found in addition toArabidopsis thalianaand we determined that purifying selection has been of great significance in maintaining the function of PKS gene family. From qRT-PCR analysis and a combination of the role of the accumulation of proanthocyanidins (PAs) in brown cotton fibers, we concluded that five PKS genes are candidate genes involved in brown cotton fiber pigment synthesis. These results are important for the further study of brown cotton PKS genes. It not only reveals the relationship between PKS gene family and pigment in brown cotton, but also creates conditions for improving the quality of brown cotton fiber.

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