In Vitro Evaluation of Anthelmintic Activity of Ethanolic Extract of Ipomoea Aquatica Forsk. In Indian Adult Earthworms

2021 ◽  
Vol 09 (07) ◽  
Author(s):  
Paonam Shyamasakhi Devi ◽  
Keyword(s):  
Anthelmintic Activity ◽  
Ethanolic Extract ◽  
In Vitro Evaluation ◽  
Ipomoea Aquatica

scholarly journals IN VITRO ANTHELMINTIC AND ANTI-AMYLASE PROPERTIES OF GARCINIA PEDUNCULATA ROXB. ETHANOLIC EXTRACT

2016 ◽  
Vol 9 (1) ◽  
pp. 189
Author(s):  
Pinky Sarmah ◽  
Nako Kobing ◽  
Jyotchna Gogoi ◽  
Ananta Madhab Dutta
Keyword(s):  
Anthelmintic Activity ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Biological Properties ◽  
Death Time ◽  
Control Sets ◽  
Point Analysis ◽  
Soxhlet Apparatus ◽  
And Control

<p><strong>Objective</strong>:<strong> </strong>The present study aims to investigate <em>in vitro</em> anthelmintic and anti-amylase properties of the ethanolic fruit extract of <em>Garcinia pedunculata</em>.</p><p><strong>Methods</strong>:<strong> </strong>For the study, mature fruits of <em>G. pedunculata</em> were collected from local markets during April-May. Fleshy pericarps of fruits were chopped into small pieces, dried and extracted by using a Soxhlet apparatus. Ethanol extract of <em>G. pedunculata</em> was used for evaluation of <em>in vitro</em> anthelmintic and anti-amylase activities. <em>In vitro</em> anthelmintic activity was evaluated in animal models, <em>Pheretima posthuma</em>, an earthworm species. <em>In vitro</em> anti-amylase activity was evaluated by using zymographic, achromic point analysis (Starch-Iodine method) and spectrophotometric method [Di Nitro Salicylic acid (DNS)–Maltose method].</p><p><strong>Results</strong>:<strong> </strong>Ethanolic extract of <em>G. pedunculata</em> showed anthelmintic activity at a concentration of 75 mg/ml, paralysis and death timing was reported at 0.62±0.26 min and 1.42±0.07 min, respectively. The reference standard (Albendazole) showed paralysis time: 2.13±0.28 min and death time: 5.12±0.29 min. In the anti-amylase study, a zymographic density analysis of <em>G. pedunculata</em> showed significant variation in band intensity as compared to Starch–Iodine achromic point analysis and DNS–Maltose method. A concentration of 1.5 mg/ml of extract showed inhibition of amylase: 67.65±1.53 % as compared to other concentrations and control sets.</p><strong>Conclusion</strong>:<strong> </strong>It could be concluded that ethanolic extract of <em>G. pedunculata</em>has biological properties which could be utilised in medicine by characterising its bioactive components.<p> </p>

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTHELMINTIC ACTIVITY OF ETHANOLIC EXTRACT OF LEAF RACHIS OF AZADIRACHTA INDICA Linn. (NEEM)

2017 ◽  
Vol 03 (01) ◽  
pp. 8-12
Author(s):  
Gavalapu Venkateswara Rao ◽  
Abhinav kanagarla ◽  
Allamraju Satyakeertana ◽  
Vinukonda Alekhya ◽  
Dammu Saipriya ◽  
...  
Keyword(s):  
Azadirachta Indica ◽  
Anthelmintic Activity ◽  
Ethanolic Extract ◽  
Phytochemical Screening

scholarly journals IN VITRO ASSESSMENT OF ANTHELMINTIC AND ALPHA-AMYLASE INHIBITION OF SCHLEICHERA OLEOSA (LOUR.) OKEN LEAF EXTRACTS

2018 ◽  
Vol 11 (9) ◽  
pp. 487
Author(s):  
Shambaditya Goswami ◽  
Ravindra Pal Singh
Keyword(s):  
Anthelmintic Activity ◽  
Ethanolic Extract ◽  
Alpha Amylase ◽  
Control Treatment ◽  
Aqueous Extracts ◽  
Leaf Extracts ◽  
Standard Drug ◽  
Isolation And Characterization ◽  
Schleichera Oleosa

Objective: The present study deals with the effects of Schleichera oleosa (Lour.) Oken leaf extracts on helminths and alpha-amylase inhibition. Identification of phytochemicals and physicochemical analysis were also performed.Methods: Different concentrations (25, 50, and 100 mg/ml) of petroleum ether, acetone, chloroform, ethanol, and aqueous extracts of the leaf were used to examine the effects. For the evaluation of in vitro anthelmintic activity, several earthworms (Eisenia fetida, Perionyx excavates, and Pheretima posthuma) and nematode (Ascaridia galli) were taken, while albendazole was used as a standard drug and Tween 80 (3%) in normal saline (0.9% NaCl) was considered as a control treatment. In vitro alpha-amylase inhibition of different extracts (10–100 mg/ml) was done spectrophotometrically by dinitrosalicylic acid - starch azure method.Results: The ethanolic extract showed the maximum presence of phytochemicals among all the extracts, which included alkaloids, tannins, flavonoids, saponin glycosides, phenolic compounds, resins, and amino acids. The outcomes of the determination of physicochemical parameters and fluorescence characters provided the satisfactory results. Significant anthelmintic activity was established by the ethanolic and aqueous extracts of the leaf among all the extracts and the responses, so observed, were dose responsive. Inhibition of alpha-amylase by ethanolic and aqueous extracts was significant with the IC50 value of 36.63 and 73.94 μg/ml, respectively, when compared to standard acarbose.Conclusion: The ethanolic extract was the more potent candidate for both the effects, and the effect of extract was best against A. galli, P. posthuma, and E. fetida at higher concentration. Isolation and characterization of therapeutic constituents would be the future interest.

scholarly journals In vitro Anthelmintic Activity of Aqueous and Ethanolic Extract of Senna italica (Caesalpiniaceae) on Three-stages of Haemonchus contortus

2020 ◽  
pp. 25-34 ◽  
Author(s):  
Gilbert Yongwa ◽  
Belga François Ngnodandi François Ngnoda ◽  
Dieudonné Ndjonka ◽  
Pierre Saotoing
Keyword(s):  
Mortality Rate ◽  
Aqueous Extract ◽  
Haemonchus Contortus ◽  
High Efficiency ◽  
Larval Mortality ◽  
Anthelmintic Activity ◽  
Ethanolic Extract ◽  
Hatching Rate ◽  
Egg Hatching

A phytochemical screening and in vitro anthelminthic activity of aqueous and ethanolic extract of Senna italica on Haemonchus contortus were conducted. Polyphenol, tannin and flavonoid contents were determined by using gallic acid and rutin. egg hatching inhibition test was carried out on fresh eggs; larval mortality test was conducted on infective larvae (L3) and adult worm mortality test was conducted. Eggs, larvae and adults worms were incubated in aqueous and ethanolic extract of S. italica at different concentrations (0.1; 0.3; 0.5; 0.7 and 1 mg/mL). All extracts of plants showed an effect on all stages of H. contortus with high efficiency variations depending on the dose used. The inhibition of eggs hatching rate increased from 8.67±1.53% to 65.67±1.15% and from 24.67±1.53% to 80±1.73% respectively for aqueous and ethanolic extract of S. italic. The larval mortality rate increased from 12.22±2.34% to 56.67±4.9% after 24 h and from 45.28±4.11% to 91.25±3.73% after 48 h for aqueous extract and from 30.07±2.84% to 70.83±6.31% after 24 h and from 48.79±3.73% to 96.25±4.79% after 48 h for ethanolic extract. After 24 h of exposure to aqueous and ethanolic extract of S. italica, the adult mortality rate varies from 55.56±9.62% to 83.33±9.62% for aqueous extract and from 61.11±9.62% to 88.89±9.62%.These in vitro results confirm the use of S. italica in traditional medicine.

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