scholarly journals UV irradiation/cold shock-mediated apoptosis is switched to bubbling cell death at low temperatures

Oncotarget ◽  
2015 ◽  
Vol 6 (10) ◽  
pp. 8007-8018 ◽  
Author(s):  
Szu-Jung Chen ◽  
Pei-Wen Lin ◽  
Hsin-Ping Lin ◽  
Shenq-Shyang Huang ◽  
Feng-Jie Lai ◽  
...  
2019 ◽  
Vol 72 (2) ◽  
pp. 155
Author(s):  
Aya Saruwatari ◽  
Kei Hashimoto ◽  
Ryota Tamate ◽  
Ryoji Usui ◽  
Hisashi Kokubo ◽  
...  

We report the photocontrollable micelle–cluster transition of an ABC-type triblock copolymer in an ionic liquid (IL). Polystyrene-b-poly(ethylene oxide)-b-poly(4-phenylazobenzyl acrylamide-r-N-isopropylacrylamide) (PSt-b-PEO-b-P(AzoBnAm-r-NIPAm)) was synthesised, where PSt is IL-phobic, PEO is IL-philic, and P(AzoBnAm-r-NIPAm) is photo- and thermoresponsive in the IL. At high temperatures, the triblock copolymer forms micelles with PSt cores; furthermore, at low temperatures, micelles self-assemble into clusters induced by the aggregation of P(AzoBnAm-r-NIPAm). Under UV irradiation, the micelles form clusters at lower temperatures than that in the dark because of the change in the solubility of P(AzoBnAm-r-NIPAm) induced by photoisomerisation of the azobenzene groups, indicating that this triblock copolymer has a photocontrollable micelle–cluster transition temperature.


Author(s):  
Francisco Alarcón Elvira ◽  
Violeta T. Pardío Sedas ◽  
David Martínez Herrera ◽  
Rodolfo Quintana Castro ◽  
Rosa María Oliart Ros ◽  
...  

Expression of the regulatory stress rpoS gene controls the transcription of cspA genes, which are involved in survival and adaptation to low temperatures. The purpose of this study was to assess the growth kinetics of naturally occurring V. parahaemolyticus in shellstock oysters and in vitro and the cold-shock-induced expression of the rpoS and cspA gene response in vitro during postharvest refrigeration. Naturally contaminated eastern oysters (Crassostrea virginica) and pathogenic (Vp-tdh) and nonpathogenic (Vp-tlh) isolates were stored at 7 ± 1 °C for 168 h and 216 h, respectively. The regulatory stress (rpos) and cold-shock (cspA) gene expressions were determined by reverse transcription PCR. At 24 h, the (Vp-tdh) strain grew faster (p < 0.05) than the (Vp-tlh) strain in oysters (λ = 0.33, 0.39, respectively) and in vitro (λ = 0.89, 37.65, respectively), indicating a better adaptation to cold shock for the (Vp-tdh) strain in live oysters and in vitro. At 24 h, the (Vp-tdh) strain rpoS and cspA gene expressions were upregulated by 1.9 and 2.3-fold, respectively, but the (Vp-tlh) strain rpoS and cspA gene expressions were repressed and upregulated by −0.024 and 1.9-fold, respectively. The V. parahaemolyticus strains that were isolated from tropical oysters have adaptive expression changes to survive and grow at 7 °C, according to their virulence.


2015 ◽  
Vol 51 (9) ◽  
pp. 1736-1738 ◽  
Author(s):  
Ryuhei Kodama ◽  
Kimio Sumaru ◽  
Kana Morishita ◽  
Toshiyuki Kanamori ◽  
Kengo Hyodo ◽  
...  

A thermally stable closed-ring isomer of a diarylethene having a sulfone group works as the SO2 gas generator upon UV irradiation; photoisomerization followed by generation of SO2 gas to induce cell death.


2006 ◽  
Vol 119 (23) ◽  
pp. 4841-4849 ◽  
Author(s):  
S. Braun ◽  
M. Krampert ◽  
E. Bodo ◽  
A. Kumin ◽  
C. Born-Berclaz ◽  
...  

1999 ◽  
Vol 181 (18) ◽  
pp. 5557-5562 ◽  
Author(s):  
T. Atlung ◽  
F. G. Hansen

ABSTRACT Expression of the dnaA gene continues in the lag phase following a temperature downshift, indicating that DnaA is a cold shock protein. Steady-state DnaA protein concentration increases at low temperatures, being twofold higher at 14°C than at 37°C. DnaA protein was found to be stable at both low and high temperatures. Despite the higher DnaA concentration at low temperatures, the mass per origin, which is proportional to the initiation mass, was the same at all temperatures. Cell size and cellular DNA content decreased moderately below 30°C due to a decrease in the time from termination to division relative to generation time at the lower temperatures. Analysis of dnaA gene expression and initiation of chromosome replication in temperature shifts suggests that a fraction of newly synthesized DnaA protein at low temperatures is irreversibly inactive for initiation and for autorepression or that all DnaA protein synthesized at low temperatures has an irreversible low-activity conformation.


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