Does ectoin affect release of allergens from pollen of wind-blown plants?

2021 ◽  
Vol 20 (6) ◽  
pp. 109-114
Author(s):  
T. M. Zheltikova ◽  
◽  
I. G. Akhapkina ◽  
M. A. Mokronosova ◽  
◽  
...  
Keyword(s):  
Nasal Cavity ◽  
Grass Pollen ◽  
Barrier Function ◽  
Light Microscope ◽  
Birch Pollen ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Enzyme Linked Immunosorbent Assay ◽  
Morphological Examination ◽  
Orchard Grass

The effect of ectoin on the release of allergens from pollen grain of wind-blown plants was studied. The birch, alder, hazel, timothy, and orchard grass pollen grains were used for this experiment. Enzyme-linked immunosorbent assay (ELISA) and morphological examination of birch pollen grains utilizing the light microscope were used. The findings indicated that ectoin does not promote the allergen release from pollen. Thus, ectoin not only improves the barrier function of the nasal mucosa and washes pollen from the nasal cavity, but also does not disturb their integrity and does not affect the release of allergens.

scholarly journals Pollen morphology of Plantago species native to Poland and their taxonomic implications

2011 ◽  
Vol 73 (4) ◽  
pp. 315-325 ◽  
Author(s):  
Małgorzata Klimko ◽  
Krystyna Idzikowska ◽  
Mariola Truchan ◽  
Anna Kreft
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscope ◽  
Pollen Morphology ◽  
Pore Diameter ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Prolate Spheroidal ◽  
Taxonomic Implications ◽  
Scanning Electron

Pollen grains of 9 species of the genus <em>Plantago</em> (Plantaginaceae), including 8 taxa native to Poland, were observed under a light microscope and a scanning electron microscope. Descriptions of grain sculpture are illustrated only SEM micrographs. The studied pollen grains were medium-sized or small, spherical or prolate spheroidal. Their sculpture was always verrucate with granulation. In the studied taxa, internal apertures had the form of pores. Their number ranged from (4)5-9(14). The pores were scattered on the surface of pollen grains. Identification features of individual taxa include: presence or absence of an annulus around each pore, annulus structure, ornamentation of the pollen grain and operculum, type of aperture membrane, number of internal pores, and pore diameter. We suggest that two new pollen grain types, characteristic of <em>P. intermedia</em> and <em>P. arenaria</em>, should be distinguished, and that <em>P. alpina</em> should be assigned to the <em>P. coronopus</em> type.

scholarly journals Pollen and sperm nuclei development in rye

2015 ◽  
Vol 46 (3) ◽  
pp. 449-457 ◽  
Author(s):  
Agnieszka Mostowska ◽  
Maria Charzyńska
Keyword(s):  
Secale Cereale ◽  
Light Microscope ◽  
Generative Cell ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Sperm Nuclei

The development of rye (<i>Secale cereale</i> L. <i>Gramineae</i>) pollen grains. was followed in the light microscope from the differentiating division in the microspore to pollen shedding. Particular attention was given to the localization of the generative cell before and during mitosis and to the change in the structure of sperm nuclei during pollen grain maturation.

Fine Structure of Pollen Grain Ektexine of Maize, Teosinte and Tripsacum

1972 ◽  
Vol 30 ◽  
pp. 226-227
Author(s):  
Umesh C. Banerjee ◽  
Elso S. Barghoorn
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Zea Mays ◽  
Fine Structure ◽  
Light Microscope ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Wild Relatives ◽  
Distilled Water ◽  
Scanning Electron

Using light microscope (LM) it was found very difficult to distinguish the ektexine (outer sculptured layer of exine) pattern of maize (Zea mays L.) pollen grains from that of its wild relatives teosinte (Euchlaena mexicana Schrad.) and tripsacum (Tripsacum spp.). At the magnifications obtained by LM, the pollen grain ektexine is faintly granular or netted. By the use of electron microscopy, however, it is possible to characterize their pollen ektexine patterns.For scanning electron microscopy (SEM) acetolysed pollen grains were used. After acetolysis the pollen samples were washed several times in glass-distilled water to remove traces of acids. Each sample was dispersed in a drop of distilled water placed on specimen holders.

scholarly journals Plants used by Megachile (Moureapis) sp. (Hymenoptera: Megachilidae) in the provisioning of their nests

2009 ◽  
Vol 69 (4) ◽  
pp. 1187-1194 ◽  
Author(s):  
MLT. Buschini ◽  
J. Rigon ◽  
J. Cordeiro
Keyword(s):  
Light Microscope ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Activity Period ◽  
Nesting Site

This study was carried out in the Parque Municipal das Araucárias, located in the municipality of Guarapuava (PR) from May (06) to April (07). Its aim was to investigate which plants are used by Megachile (Moureapis) sp in provisioning of larvae, and to verify if this bee is oligolectic or polilectic. The collection of the plants was carried out during the bees activity period and in an area covering a 500 m radius from where the nests had been built. A sample of pollens from founded nests and from flowers was used to make permanent slides using the acetolysis method and with the aid of a light microscope, all pollen grains were examined in order to identify the plants used by this bee. A total of 80 pollen grain slides, from 16 nests were analysed. Although 99 plants were collected close to the bee's nesting site, in nest slides, the pollen of Ludwigia peruviana (36%) and Ludwigia sericea (63.6%) (Onagraceae) accounted for 99.6% of the total amount of pollen collected. With these results we are able to conclude that Megachile (Moureapis) sp is an oligolectic species and that these plants species are their important pollen source.

scholarly journals Morphological study of pollen-grains for the wild species of the genus Erysimum L. (Crucifereae) in Iraq

2012 ◽  
Vol 9 (4) ◽  
pp. 569-574
Author(s):  
Baghdad Science Journal
Keyword(s):  
Outer Layer ◽  
Wild Species ◽  
Light Microscope ◽  
Morphological Study ◽  
Pollen Grains ◽  
Pollen Grain ◽  
The Other ◽  
Oblate Spheroidal ◽  
Small Pollen ◽  
Thickened Wall

Pollen grains morphology have been studied for the wild species of the genus Erysimum L. which belong to Crucifereae family in Iraq. These species are E. filifolium Boiss. et Hausskn., E. oleifolium J. Gay, E. repandum L., E. eginense Hausskn. ex Bornm., E. aucheranum J. Gay, E. cheiranthoides L., E. alpestre Ky. ex Boiss., E. kurdicum Boiss. et Hausskn., E. tenellum DC., E. strophades Boiss., E. gladiiferum Boiss. et Hausskn., E. nasturtioides Boiss. et Hausskn. The study was performe by using light microscope . The study reveal that there was only one type of pollen grain named Tricoplate in all studied species . The study also demonstrated that there were differences among pollen grains morphology . The species E. kurdicum , E. alpestre , and E. strophades have shown Oblate form , while the species E. oleifolium , E. repandum , E. filifolium , E. aucheranum , and E. tenellum have Oblate-spheroidal form . On the other hand the species E. eginense , E. gladiiferum , E. cheiranthoides , and E. nasturtioides have Sub-oblate pollen . It was also noticed that the outer layer of pollen grains has more thickened wall than the inner layer . The data also shown that there were differences in pollen grains sizes . The species E. oleifolium , E. strophades , E. filifolium , E. gladiiferum , E. E. kurdicum , E. aucheranum , E. tenellum , E. repandum , and E. nasturtioides have very small pollen grains , while they were small to middle in the other species .The data getting from this study showed important taxonomical characters to classifying the studied species

scholarly journals Species Identification of Pinus Pollen Found in Belukha Glacier, Russian Altai Mountains, Using a Whole-Genome Amplification Method

Forests ◽  
2018 ◽  
Vol 9 (8) ◽  
pp. 444
Author(s):  
Fumio Nakazawa ◽  
Yoshihisa Suyama ◽  
Satoshi Imura ◽  
Hideaki Motoyama
Keyword(s):  
Species Identification ◽  
Whole Genome Amplification ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Pinus Sibirica ◽  
Whole Genome ◽  
Accurate Identification ◽  
Closely Related Species ◽  
Genome Amplification ◽  
Amplification Method

Pollen taxa in sediment samples can be identified based on morphology. However, closely related species do not differ substantially in pollen morphology, and accurate identification is generally limited to genera or families. Because many pollen grains in glaciers contain protoplasm, genetic information obtained from pollen grains should enable the identification of plant taxa at the species level. In the present study, species identification of Pinus pollen grains was attempted using whole-genome amplification (WGA). We used pollen grains extracted from surface snow (depth, 1.8–1.9 m) from the Belukha glacier in the summer of 2003. WGA was performed using a single pollen grain. Some regions of the chloroplast genome were amplified by PCR, and the DNA products were sequenced to identify the pollen grain. Pinus includes approximately 111 recognized species in two subgenera, four sections, and 11 subsections. The tree species Pinus sibirica and P. sylvestris are currently found at the periphery of the glacier. We identified the pollen grains from the Belukha glacier to the level of section or subsection to which P. sibirica and P. sylvestris belong. Moreover, we specifically identified two pollen grains as P. sibirica or P. cembra. Fifteen species, including P. sibirica, were candidates for the remaining pollen grain.

scholarly journals Pollen characters as taxonomic evidence in some species of Dipsacaceae from Iran

2017 ◽  
Vol 24 (2) ◽  
pp. 129-136 ◽  
Author(s):  
Ebadi-Nahari Mostafa ◽  
Nikzat-Siahkolaee Sedigheh ◽  
Eftekharian Rosa
Keyword(s):  
Pollen Morphology ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Pollen Type ◽  
Upgma Tree ◽  
Pollen Characters ◽  
Exine Ornamentation ◽  
The Family ◽  
The World ◽  
Plant Taxon

Pollen morphology of nine species representing four genera: Cephalaria Schrad, Dipsacus L., Pterocephalus Vaill. and Scabiosa L. of the family Dipsacaceae in Iran has been investigated by means of scanning electron microscopy (SEM). The results showed that pollen grains were triporate and tricolpate. The pollen type of Scabiosa rotata Bieb. (tri- and tetraporate) is the first report in the world. The sizes of pollen grains fall into the classification group magna (pollen grain diameter 50–100 μm). Pollen shapes vary from preoblate to prolate and their polar views were triangulate and lobate. The exine ornamentation varies from gemmate in S. rotata to spinulate in the rest studied species. Species of Scabiosa have been dispersed in UPGMA tree that this confirmed the previous studies about taxonomic problems and species complexity in this genus. These results show the transfer of the some Scabisoa species to Lomelosia Raf. based on palynological characters. Pollen morphology of the family is helpful at the generic and specific level.Bangladesh J. Plant Taxon. 24(2): 129–136, 2017 (December)

scholarly journals Pollen, Tapetum, and Orbicule Development inColletia paradoxaandDiscaria americana(Rhamnaceae)

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
M. Gotelli ◽  
B. Galati ◽  
D. Medan
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Ultrastructural Changes ◽  
Transmission Electron ◽  
The Family ◽  
Tapetal Cells ◽  
Grain Formation

Tapetum, orbicule, and pollen grain ontogeny inColletia paradoxaandDiscaria americanawere studied with transmission electron microscopy (TEM). The ultrastructural changes observed during the different stages of development in the tapetal cells and related to orbicule and pollen grain formation are described. The proorbicules have the appearance of lipid globule, and their formation is related to the endoplasmic reticulum of rough type (ERr). This is the first report on the presence of orbicules in the family Rhamnaceae. Pollen grains are shed at the bicellular stage.

scholarly journals Prunus necrotic ringspot virus Early Invasion and Its Effects on Apricot Pollen Grain Performance

2007 ◽  
Vol 97 (8) ◽  
pp. 892-899 ◽  
Author(s):  
Khalid Amari ◽  
Lorenzo Burgos ◽  
Vicente Pallas ◽  
María Amelia Sanchez-Pina
Keyword(s):  
Developmental Stages ◽  
Generative Cell ◽  
Pollen Grains ◽  
Growth Zone ◽  
Pollen Tubes ◽  
Climatic Conditions ◽  
Pollen Grain ◽  
Ringspot Virus ◽  
Prunus Necrotic Ringspot Virus

The route of infection and the pattern of distribution of Prunus necrotic ringspot virus (PNRSV) in apricot pollen were studied. PNRSV was detected both within and on the surface of infected pollen grains. The virus invaded pollen during its early developmental stages, being detected in pollen mother cells. It was distributed uniformly within the cytoplasm of uni- and bicellular pollen grains and infected the generative cell. In mature pollen grains, characterized by their triangular shape, the virus was located mainly at the apertures, suggesting that PNRSV distribution follows the same pattern as the cellular components required for pollen tube germination and cell wall tube synthesis. PNRSV also was localized inside pollen tubes, especially in the growth zone. In vitro experiments demonstrated that infection with PNRSV decreases the germination percentage of pollen grains by more than half and delays the growth of pollen tubes by ≈24 h. However, although PNRSV infection affected apricot pollen grain performance during germination, the presence of the virus did not completely prevent fertilization, because the infected apricot pollen tubes, once germinated, were able to reach the apricot embryo sacs, which, in the climatic conditions of southeastern Spain, mature later than in other climates. Thus, infected pollen still could play an important role in the vertical transmission of PNRSV in apricot.

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