scholarly journals Isolation and identification of pathogens causing stem rot of the fig tree (Ficus carica)

Author(s):  
Sheyla Natali Jahén-Rivera ◽  
Olga Gómez-Rodríguez ◽  
David Espinosa-Victoria

En México, el sistema de producción intensivo de higo (<em>Ficus carica</em>) var. “Nezahualcoyotl” requiere de densidades de 12,500 plantas ha-1 para rendimientos superiores a 100 t ha-1. La propagación asexual de esta variedad mediante estacas no está exenta de enfermedades. El objetivo de esta investigación fue identificar los agentes causales de la pudrición de estacas de la variedad “Nezahualcoyotl” y generar la correspondiente escala de severidad. La patogenicidad de los aislamientos se corroboró a través de los postulados de Koch. La secuenciación de las regiones ITS1-ITS4 indicó que los aislamientos estuvieron altamente emparentados con <em>Fusarium solani</em>, <em>Alternaria alternata</em> y <em>Pythium ultimum</em>. Se reporta por primera vez una escala de severidad con cinco niveles de daño inducidos por <em>F. solani</em> y <em>A. alternata</em> y tres por <em>P. ultimum</em>. La inoculación por separado con <em>F. solani</em> y <em>A. alternata</em> promovió el 100% de daño de la corteza entre los 21-25 días después de la inoculación (DDI). La coinoculación de los tres patógenos indujo más tempranamente (entre los 11-13 DDI) la necrosis de la epidermis (85%) y de la corteza (80%). La escala de severidad será un valioso auxilio en la cuantificación y seguimiento de la pudrición del tallo de la higuera.

2020 ◽  
Author(s):  
Elena Sasco ◽  

The study established different reactions of the growth characters of the autumn common wheat seedlings to the treatment of seeds of genotypes Moldova 614, Moldova 66 and L Selania / Accent with the culture filtrates of the strains of Alternaria alternata, Drechslera sorokiniana and Fusarium solani. The 3 strains of the F. solani pathogen produced concomitant repression of root and stem in Moldova 614 and Moldova 66, but differentiated in L Selania / Accent, being identified as the most aggressive in this study. The highest sensitivity was recorded by L Selania / Accent in the case of the root under the action of Alternaria alternata strains.


Irriga ◽  
2020 ◽  
Vol 25 (3) ◽  
pp. 590-602
Author(s):  
Jonathas Batista Goncalves Silva ◽  
Mauro Aparecido Martinez ◽  
Luiz Carlos Chamhum Salomão ◽  
Paulo Roberto Cecon ◽  
Antonio Teixeira de Matos ◽  
...  

EFFECTS OF DAIRY FARM WASTEWATER USE IN CULTIVATION ON FIG TREE (FICUS CARICA L.)     JONATHAS BATISTA GONCALVES SILVA1; MAURO APARECIDO MARTINEZ2; LUIZ CARLOS CHAMHUM SALOMÃO3; PAULO ROBERTO CECON4; ANTONIO TEIXEIRA DE MATOS5 E LEONARDO DUARTE BATISTA DA SILVA6   1Departamento de Engenharia Sanitária e Ambiental, UFJF, Rua José Lourenço Kelmer, s/n,Bairro São Pedro, 36036-900, Juiz de Fora, MG, Brasil, [email protected]. 2Departamento de Engenharia Agrícola, UFV, Av. Peter Henry Rolfs, s/n, 36570-900, Viçosa, MG, Brasil, [email protected] 3Departamento de Fitotecnia, UFV, Av. Peter Henry Rolfs, s/n, 36570-900, Viçosa, MG, Brasil, [email protected] 4Departamento de Estatística, UFV, Av. Peter Henry Rolfs, s/n, 36570-900, Viçosa, MG, Brasil,[email protected]. 5Departamento de Engenharia Sanitária e Ambiental, UFMG, Avenida Presidente Antônio Carlos, 6627, Bairro Pampulha,31270-901, Belo Horizonte, MG, Brasil,[email protected] 6Departamento de Engenharia, UFRRJ, Rodovia BR 465,km 7, s/n,23890-000, Seropédica, RJ, Brasil, [email protected]     1 ABSTRACT   The purpose of this study was to evaluate the effects of dairy farm wastewater (DFW) use on fig tree growth, production, on fig health standard and on nutrient concentration in fig tree leaves. The study was developed in the Integrated Agroecological System area, in Seropédica (RJ, Brazil) between June  2011 and May  2012. The applied fertilizer formulations were: Formulation 1, 100% of nitrogen dose recommended for fig tree supplied by fertilizing with castor bean cake (CB); Formulation 2, 50% of nitrogen dose supplied by DFW application and 50% of nitrogen dose supplied by CB; Formulation 3, 75% of nitrogen dose supplied by DFW application and 25% of nitrogen dose from CB; Formulation 4, 100% of nitrogen dose supplied by DFW application.Data were submitted to analysis of variance and the averages were compared by Tukey’s test at 10% probability. The results demonstrated that branches length, number of leaves per branch, number of fruits, production and yield were lower in plants submitted to Formulation 4. Contamination of fruits by thermotolerant coliforms or Salmonellasp did not occur after DFW use as fertilizer. The results showed that the use of DFW in fig tree cultivation was sufficient to provide the nutritional needs of plants, as regards macronutrients and Fe.   Keywords: fertirrigation, environmental impact, final disposal of effluents and crop nutrition.     SILVA, J.B. G.; MARTINEZ, M. A.; SALOMÃO, L. C. C.; CECON,P. R.; MATOS, A. T.; SILVA, L. D. B. EFEITOS DO USO DE ÁGUA RESIDUÁRIA DE BOVINOCULTURANO CULTIVO DA FIGUEIRA (FICUS CARICA L.)         2 RESUMO   Objetivou-se com este trabalho avaliar os efeitos do uso da água residuária de bovinocultura de leite (ARB) no crescimento, produção, padrão fitossanitário dos frutos e na concentração de nutrientes nas folhas da figueira. As formulações de adubação aplicadas foram: Adubação 1 - 100% da dose de nitrogênio fornecida pela adubação com torta de mamona (TM); Adubação 2 - 50% da dose de nitrogênio comaplicação de ARB e 50% com TM; Adubação 3 - 75% da dose de nitrogênio com aplicação de ARB e os outros 25% da dose com TM; Adubação 4 - 100% da dose de nitrogênio com aplicação da ARB. Os resultados foram submetidos à análise de variância e as médias comparadas utilizando-se o Teste de Tukey a 10% de probabilidade. Diante dos resultados verificou-se que comprimento dos ramos, o número de folhas por ramos, o número de frutos, a produção e a produtividade foram menores nas plantas submetidas à Adubação4. Não ocorreu contaminação dos frutos por coliformes termotolerantes e Salmonella sp. Diante dos resultados concluiu-se que o uso de ARB no cultivo da figueira não proporciona deficiência nutricional às plantas no que se refere aos macronutrientes (N, Ca, Mg, K e P).   Palavras-chave: fertirrigação, impacto ambiental, disposição final de efluentes, nutrição vegetal.


2013 ◽  
Vol 22 (1) ◽  
pp. 39-46
Author(s):  
Shamim Shamsi ◽  
Najmun Naher

A total of nine fungi were isolated from two ornamental angiosperms, namely Hemerocallis fulva L. and Pancratium verecundum Ant. belong to Liliaceae. Three species of fungi were found to be associated with the leaf of Hemerocallis fulva. The fungi were Colletotrichum capsici, (Syd.) Bull. & Bisby, Colletotrichum dematium (Pers. Ex Fr.) and Glomerella montana (Sacc.)v Arx & E. Muller. Six species of fungi, namely Alternaria alternata (Fries) Keissler, Colletotrichum orbiculare (Berk & Mont.) Arex., Curvularia clavata Jain, Fusarium solani (Mort.) Sacc., Lasiodiplodia theobromae (Pat.) Griffon and Maubol and Macrophomina phaseolina (Tassi) Goid were found to be associated with P. verecundum. Among the fungal species Glomerella montana is new record for Bangladesh. Dhaka Univ. J. Biol. Sci. 22(1): 39-46, 2013 (January)


2003 ◽  
Vol 44 (4) ◽  
pp. 412-414 ◽  
Author(s):  
Jin Sun Kim ◽  
Yeon Ok Kim ◽  
Hyun Ju Ryu ◽  
Yeon Sig Kwak ◽  
Ji Yeon Lee ◽  
...  

Author(s):  
RANDA ABDOU ◽  
MOHAMED DAWOUD

Objective: Endophytes are widely spread in the plant kingdom and represent a very promising source of biologically active natural products. The medicinal plant Bidens bipinnata Lin. (Asteraceae) which is known for its anti-inflammatory, antifungal and antitumor effects has been chosen for the investigation of its endophyte to search for bioactive metabolites. Methods: An endophytic Alternaria alternata species was isolated from the leaves of the plant B. bipinnata Lin. To investigate the metabolic profile of this endophytic fungus it was cultivated in several culture media as static and shaken culture. The antimicrobial and cytotoxic activities of the ethyl acetate extracts of the fungus were examined. Extracts exhibiting highest antimicrobial activities in agar diffusion assay and cytotoxicity against HeLa cancer cell line were subjected to activity-guided chromatographic fractionation for the identification of bioactive metabolites. A cytotoxic assay was performed on the isolated compounds against HeLa cancer cell lines as well as cytostatic activity tests against HUVEC and K-562 cell lines. Results: Chromatographic fractionation resulted in the isolation and identification of alternariol and tentoxin from the extract of the fungus cultivated in medium M5 while sterigmatocystin was isolated in addition to alternariol and tentoxin from the extract of the fungus grown in medium M25. Both alternariol and sterigmatocystin proved to be of moderate cytotoxicity and weak cytostatic activity with alternariol showing higher cytotoxic activity than sterigmatocystin. Highest cytotoxicity against HeLa cell lines was observed for tentoxin with a CC50 of 22.5 µg/ml. Conclusion: This study presents the isolation and identification of the bioactive metabolites alternariol, sterigmatocystin and tentoxin from the endophyte A. alternata in addition to the antifungal activity of the strain extract as well as the cytotoxic and cytostatic activities of the isolated metabolites against HeLa, HUVEC and K-562 cell lines, respectively.


1993 ◽  
Vol 73 (1) ◽  
pp. 365-367 ◽  
Author(s):  
J. C. Tu ◽  
S. J. Park

A bean (Phaseolus vulgaris) line, A - 300, resistant to Rhizoctonia solani and Fusarium oxysporum was introduced into Ontario from Colombia. The results of tests conducted in a root-rot nursery, in a greenhouse and in a growth room showed that this bean line is resistant to Fusarium solani f. sp. phaseoli and Pythium ultimum. Key words: Bean, Phaseolus vulgaris, root rot resistance


2018 ◽  
Vol 33 (1) ◽  
pp. 81
Author(s):  
Rafael Augusto Ferraz ◽  
Sarita Leonel ◽  
Jackson Mirellys Azevedo Souza ◽  
Marcelo de Souza Silva ◽  
Bruno Henrique Leite Gonçalves

O presente trabalho teve como objetivo avaliar o desempenho propagativo de estacas semilenhosas de variedades de figueira, com o uso de ácido indolbutírico. As variedades avaliadas foram Roxo de Valinhos, White Genova, PI – 189 e Troiano e a dosagem empregada do ácido indolbutírico foi 2000 mg L-1. O delineamento experimental utilizado foi o inteiramente ao acaso, em esquema fatorial 4 x 2 (variedades x com e sem aplicação do regulador vegetal), com cinco repetições e dez estacas por parcela experimental. Foram avaliadas a porcentagem de estacas vivas, de estacas enraizadas e de estacas brotadas, número de brotos, comprimento da maior raiz (cm), número de folhas, volume da raiz (cm3), peso seco da raiz (mg) e peso seco das folhas (mg). As estacas que não receberam a aplicação do regulador vegetal tiveram maior porcentagem de estacas vivas, enraizadas e brotadas, com exceção das variedades Roxo de Valinhos e Troiano, que não apresentaram diferença na aplicação. Mediante esses resultados, foi possível concluir que não é recomendado o uso do ácido indolbutírico para essas variedades, na época de coleta das estacas correspondente ao mês de agosto.PALAVRAS-CHAVE: Ficus carica L., propagação, regulador de crescimento FIG TREE VARIETIES CUTTINGS ROOTING USING INDOLEBUTYRIC ACID ABSTRACT: The present study aimed to evaluate the propagative performance of fig trees cuttings with application of indolebutyric acid (IBA). The evaluated varieties were “Roxo of Valinhos, White Genova, PI -189 and Troiano and the dosage of indolebutyric acid used was 2000 mg L-1. The experimental design used was entirely random, in factorial scheme 4 x 2 (varieties x with or without application of plant regulator), with five replications and ten cuttings per plot. It was evaluated the percentage of live cuttings, rooted cuttings and sprouted cuttings, number of shoots, length of the largest root (cm), number of leaves, root volume (cm3), root dry weight (mg) and leaves dry weight (mg). The cuttings that did not receive treatment with indolebutyric acid had  higher percentage of live, rooted and sprouted cuttings, except for  Roxo of Valinhos” and Troiano varieties, which showed no difference among treatments . Based on the results, it is possible to conclude that the use of indolebutyric acid for such varieties is not recommended in August, month of  cuttings harvest in this study. KEYWORDS: Ficus carica L., propagation, growth regulator.


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