scholarly journals Functional Group Analysis of Germinated Millets and Legumes

Author(s):  
S Reginold Jebitta ◽  
SC Venkatram ◽  
S Mohamed Aneesh ◽  
R Pasupathi

Germinated millets and legumes are rich in nutritional significance like calcium, iron, proteins, vitamins, etc. This study aims to find the active functional groups and compounds present with their bond characteristics based on the peak obtained from FT-IR for germinated millets and legumes that are subjected for shade drying and tray drying at 700c. Generally, Millets and legumes are used for specialty foods like health mix, etc or as a part of generally food products due to their increased health significance. In this study, the findings are the presence of characteristic functional groups like carboxylic acids, aromatic rings, disulphide, esters, alkanes, amines, nitro compounds, sulphates, oxime, psosphine, thiocarbonyl, silane, amides, sulfate, alkenes, etc. There is some difference in functional groups based on the method of drying. These results help identify the bioactive compound which places a major role in our nutritional health aspects. These germinated products could add value to our daily diet.

2021 ◽  
Vol 9 (2) ◽  
pp. 99-104
Author(s):  
Jeanne Dewi Damayanti ◽  
Ririn Azmilia ◽  
Zul Ainun ◽  
Nur Amin R. ◽  
M. Ilham Nurdin

Lycopene is a red pigment found in tamarillo with its function as an antioxidant that protects body cells from the negative effects of free radicals so that they do not trigger diseases, especially cancer and premature aging. This study was intended to improve the quality of tamarillo by isolating lycopene from tamarillo through an extraction process with chloroform as a solvent by maceration for 3 days at room temperature. The lycopene extract obtained was dissolved by means of a rotary evaporator at 40 oC under vacuum pressure and oven temperature at 60 oC. The lycopene obtained was analyzed by Gas Chromatography-Mass Spectrometry and the crystal structure of lycopene was characterized by Fourier Transform Infra-Red Spectroscopy. GCMS results showed that 0.21 g of lycopene was successfully isolated from 100 g of dried red tamarillo fruit powder. Functional group analysis using FT-IR at a wavelength of 978.23 cm-1 showed the R-CH=CH-R group; the -CH3- group of 1371.43 cm-1; the 1460.16 cm-1 indicates the bending vibration of -CH2-; the C=C chain of 1656.91 and 1745.64 cm-1; and the   C-H of 2856.67 and 2926.11 cm-1 of the lycopene chain.


2021 ◽  
Vol 13 (2) ◽  
pp. 10904
Author(s):  
Jeacoumar KAVIPRIYA ◽  
Vaithilingam RAVITCHANDIRANE

The nutritional composition and functional groups of the edible portion of the most often utilized cuttlefish, Sepia pharaonis of Puducherry coastal waters, India was evaluated. Moisture is the basic component of the mantle with 82.45±0.15% followed by a good source of 14.5±0.1% protein. The ash, lipid, and carbohydrates were found less than 1%. The energetic value of the mantle of cuttlefish S. pharaonis is calculated as 6.06 KJ/g. Na and K were found in considerable quantities of 2772 and 3247 mg/kg respectively and the Na/K ratio (0.85) was less than 1. The amount of Zn, Mn, B, Al, Sr, and As was found to be relatively high when compared to a low or insignificant level of Cu, Mo, Se, Cr, Ni, Pb, and Hg. The fatty acid profile analysis revealed that it contains 71.67% saturated fatty acids,17.87% polyunsaturated fatty acids and 10.38% monounsaturated fatty acids. In all three types, the C16:0 palmitic acid (40.29%), C18:0 stearic acid (16.54%), and C22:6 docosahexaenoic acid (13.52%) represented as the dominant fatty acids. The FT-IR analysis disclosed the presence of various functional groups belonging to protein, lipids, and polysaccharides. Thus, the present study proved that the cuttlefish S. pharaonis is considered as a potential nutraceutical and the right choice of food for greater and wider human consumption. 


2018 ◽  
Vol 13 (12) ◽  
pp. 1934578X1801301 ◽  
Author(s):  
Roopa Gaonkar ◽  
Pramod K Avti ◽  
Gurumurthy Hegde

Terpenoids such as geraniol and citral are known to have antibacterial, antifungal and anti-cancerous properties; however, their mechanism of action is least understood. In this study, the antifungal mechanism of monoterpene alcohol (geraniol) and monoterpene aldehyde (citral) was studied using Saccharomyces cerevisiae throughout 120 h duration and over a maximum tolerable dosage of 0.5% (v/v). Cell growth studies using optical density readings at 600 nm (ODλ=600nm), Cell viability using MTT assay, Na+/K+ leakage into media, osmotic stress using flame photometry, detection of metabolites like dehydroergosterol (DHE), H2O2 using fluorescence spectroscopy, changes in functional group analysis using FT-IR, elemental analysis using EDAX, DNA damage using gel electrophoresis and cellular structural changes using SEM were carried out. Growth inhibition studies using ODλ=600nm and MTT assays suggest that both geraniol and citral inhibited the growth kinetics significantly up to 0.1% v/v at and significantly increased beyond 0.1% at all the studied time points. Both the monoterpenoids induced the osmotic stress in S. cerevisiae measured as a change in reduction in pH, [H]+ concentration, with elevation in [Na]+ and [K]+ leakage into the media. Geraniol treatment reduced the levels of metabolites, dehydroergosterol (DHE) and H2O2, in a time-dependent manner whereas citral only affected their levels at 120 h. Energy dispersive X - ray spectroscopy (EDAX) studies suggest that both the monoterpenoids treatment differentially modulated the cellular elemental contents. Geraniol (0.03% v/v) and not the citral treatment induced DNA damage. Cellular structural analysis showed that both monoterpenoids have differential damaging effects to S. cerevisiae. This study shows that having an aldehyde and alcohol group on the terpenoids strongly affect the anti-fungal activity.


2021 ◽  
Vol 924 (1) ◽  
pp. 012089
Author(s):  
Nurhayati ◽  
R Kusumawati ◽  
Suryanti

Abstract Research on fish collagen is now growing rapidly as the use of collagen in industry increases. Collagen extraction begins with the removal of non-collagen proteins using bases to maximize the extraction process. This research aims to determine the effect of differences in NaOH concentrations on the characteristics of tilapia skin collagen. NaOH in collagen extraction serves to remove alkaline soluble proteins to optimize the collagen extraction process. The bases used were NaOH with the concentration of 0.5, 1.0, and 1.5%. The extraction was carried out using the acid method. Using SEM, observation parameters for crude collagen from the tilapia skin include collagen yield, functional group analysis, lightness, and surface morphology. The results of functional groups analysis showed that the collagen obtained in all treatments had typical collagen characteristics, i.e., amide A, amide B, amide I, amide II, and amide III. The non-collagen deproteination treatment with 0.5% NaOH could produce better collagen than the 1.0 and 1.5% concentrations, as indicated by the highest yield (20.42%) and lightness (93.22). Morphological analysis showed that the collagen extracted has an irregular branched fiber structure.


Author(s):  
Viki Fathurohman ◽  
Darmawan Alisaputra ◽  
Endaruji Sedyadi

Bioplastics can be made with chitosan as a base material with a sorbitol plasticizer and avocado-based seed starch. This study tries to discuss the comparison of avocado seeds to bioplastic biodegradability required based on weight loss in soil media. This research was carried out in empathic, namely making avocado starch, making bioplastics, analyzing bioplastic functional groups, and testing biodegradation of bioplastics in soil media. Variations used are avocado seeds used are 0; 0.1; 0.3; 0.5; 0.7; and 1.4 grams. Bioplastic functional group analysis was performed using FTIR. Bioplastic biodegradability in soil media. Bioplastics are 76% degraded within 12 days in the soil. This shows that bioplastics can be biodegradable.


F1000Research ◽  
2020 ◽  
Vol 8 ◽  
pp. 2071 ◽  
Author(s):  
Norberto Sánchez-Cruz ◽  
B. Angélica Pilón-Jiménez ◽  
José L. Medina-Franco

Background: Natural product databases are important in drug discovery and other research areas. An analysis of its structural content, as well as functional group occurrence, provides a useful overview, as well as a means of comparison with related databases. BIOFACQUIM is an emerging database of natural products characterized and isolated in Mexico. Herein, we discuss the results of a first systematic functional group analysis and global diversity of an updated version of BIOFACQUIM. Methods: BIOFACQUIM was augmented through a literature search and data curation. A structural content analysis of the dataset was performed. This involved a functional group analysis with a novel algorithm to automatically identify all functional groups in a molecule and an assessment of the global diversity using consensus diversity plots. To this end, BIOFACQUIM was compared to two major and large databases: ChEMBL 25, and a herein assembled collection of natural products with 169,839 unique compounds. Results: The structural content analysis showed that 15.7% of compounds and 11.6% of scaffolds present in the current version of BIOFACQUIM have not been reported in the other large reference datasets. It also gave a diversity increase in terms of scaffolds and molecular fingerprints regarding the previous version of the dataset, as well as a higher similarity to the assembled collection of natural products than to ChEMBL 25, in terms of diversity and frequent functional groups. Conclusions: A total of 148 natural products were added to BIOFACQUIM, which meant a diversity increase in terms of scaffolds and fingerprints. Regardless of its relatively small size, there are a significant number of compounds and scaffolds that are not present in the reference datasets, showing that curated databases of natural products, such as BIOFACQUIM, can serve as a starting point to increase the biologically relevant chemical space.


Sign in / Sign up

Export Citation Format

Share Document