scholarly journals Association of SSCP Polymorphisms of HSP70 Gene with Physiological, Production and Reproduction Performance in Sahiwal and Crossbred Cows

2021 ◽  
Author(s):  
J. Sai Prasanna ◽  
S.T. Viroji Rao ◽  
M. Gnana Prakash ◽  
Suresh Rathod ◽  
P. Kalyani ◽  
...  
Keyword(s):  
Heat Shock ◽  
Milk Yield ◽  
Single Strand Conformation Polymorphism ◽  
Temperature Tolerance ◽  
Hsp70 Gene ◽  
Crossbred Cows ◽  
Reproduction Traits ◽  
Sahiwal Cows ◽  
Cellular Tolerance ◽  
Shock Proteins

Background: Cellular tolerance to heat stress is mediated by heat shock proteins (HSPs). The HSPs act as molecular chaperones and are transcribed in response to stress. Among different families of these proteins, HSP70 is considered to be related to the development of temperature tolerance. Unraveling polymorphism in heat shock protein genes could be a step towards the identification of genetic markers for selecting heat-tolerant cattle. Methods: The present study was carried out in Sahiwal (n=50) and Crossbred cows (n=50) with the objective to identify polymorphisms in HSP70 gene. Two fragments (295 and 220 bp) of HSP70 gene were subjected to Polymerase Chain Reaction-Single-Strand Conformation Polymorphism (PCR-SSCP) technique. Statistical analysis was performed to study the association of each SSCP genotype on physiological, production and reproduction traits in Sahiwal and crossbred cows using the univariate GLM model of SPSS 25. Result: The PCR-SSCP of 295 bp fragment of HSP70 gene revealed two genotypes AA and AB in Sahiwal cows and two genotypes AA and AC in crossbred cows. The association analysis revealed that genotype AA had higher peak milk yield in Sahiwal cows while the same genotype had higher total lactation milk yield, lower service period and calving interval in crossbred cows. The 220 bp fragment was found to be monomorphic in both Sahiwal and crossbred cows.

scholarly journals Identification of Polymorphism within Exon 8 of Bovine HSP90AA1 Gene using PCR-SSCP Technique

2021 ◽  
Vol 12 (6) ◽  
pp. 611-616
Author(s):  
J. Sai Prasanna ◽  
◽  
S. T. Viroji Rao ◽  
M. Gnana Prakash ◽  
Suresh Rathod ◽  
...  
Keyword(s):  
Reproductive Traits ◽  
Experimental Period ◽  
Single Strand Conformation Polymorphism ◽  
Crossbred Cows ◽  
Self Defense ◽  
Service Period ◽  
Reproduction Traits ◽  
Sahiwal Cows ◽  
Stressed Cells ◽  
Shock Proteins

Heat shock proteins (HSPs) are a type of molecular chaperones that aid in the recovery of stressed cells and serve as a major system for intracellular self-defense. A study was conducted during the year 2018–19 at College of Veterinary Science Rajendranagar, Hyderabad, Telengana State, India to find polymorphisms in exon 8 of the bovine HSP90AA1 gene in Sahiwal (n=50) and crossbred (n=50) cows. Blood samples were collected from the experimental animals and genomic DNA was isolated. Physiological parameters like body temperature and respiration rate for each animal were taken during the experimental period and the heat tolerance coefficient was calculated. The data on production and reproduction traits were obtained from the history sheets of the animals. To detect the polymorphism, a 539 bp fragment of the HSP90AA1 gene covering exon 8 was subjected to the Polymerase Chain Reaction-Single-Strand Conformation Polymorphism (PCR-SSCP) technique.The PCR-SSCP of exon 8 of HSP90AA1 gene yielded two genotypic patterns AA and AB corresponding to two allelic variants with frequencies of 0.85, 0.15, 0.81 and 0.19 in Sahiwal and crossbred cows, respectively. The PCR-SSCP patterns obtained were correlated with the physiological, productive, and reproductive traits in both Sahiwal and crossbred cows. The association analysis of SSCP patterns of the exon 8 of HSP90AA1 gene revealed non-significant effect in Sahiwal cows, although the AB genotype had a significantly longer service period in crossbred cows.

Detection of Subclinical Ketosis in Urine of Cattle and Buffaloes

2021 ◽  
Author(s):  
Nishtha Kushwah ◽  
Mahendra Singh ◽  
Ashwani Kumar Roy
Keyword(s):  
Milk Production ◽  
Glucose Level ◽  
Milk Yield ◽  
Ketone Bodies ◽  
Calculated Data ◽  
Test Strip ◽  
Urine Samples ◽  
Subclinical Ketosis ◽  
Crossbred Cows ◽  
Sahiwal Cows

Background: Subclinical ketosis (SCK) in dairy cows is a common metabolic disorder and a strong determinant of the health and performance of cows throughout lactation. Considerable amount of milk loss takes place with SCK incidence in dairy animals. Greater benefits are to be gained by monitoring the cows for SCK. The current study was aimed to find out incidence of SCK by urine test strip (Keto-Diastix). Methods: The present investigation was carried out on crossbred and Sahiwal cows and Murrah buffaloes (n=180) of the institute herd and rural dairy farms (n=97) around Karnal. The urine samples were tested for subclinical ketosis (SCK) 5-60 days postpartum from August 2019 to March 2020. Meteriological data on wet and dry bulb temperature and ambient temperature was collected and THI was calculated. Data of SCK incidence was classified based on stages of early lactation (5-20, 21-40 and 41-60d), parity (I, II, III and above), seasons (hot-humid, autumn, winter and spring) and milk production level ( less than 10kg, 10-20 kg and greater than 20 kg/d). Milk yield and environment variables were recorded during the experiment. Urine ketone and glucose level was detected by keto-Diastix to find out incidence of SCK. Result: Mean level of ketone bodies in urine was higher (P less than 0.01) in high yielding cows as compared to the medium yielders; however it did not vary in buffaloes. Ketone bodies in urine were high (P less than 0.01) in crossbred cows than that of buffaloes and Sahiwal cows. Urine glucose level did not vary among cows and buffaloes. It is concluded that high producing crossbred cows are more vulnerable to SCK between 41-60d of lactation and in III parity and above due to peak milk production. Sahiwal cows and buffaloes suffer less from SCK probably due to medium level of milk production. Factors like high milk yield, higher parity and hot-humid stress makes the animal more vulnerable to SCK. The screening of urine samples of animals at monthly intervals may be practiced by the farmers to monitor the incidence of SCK.

Association Analysis of Heat Shock Protein 70 Gene Polymorphisms in Schizophrenia

2009 ◽  
Vol 24 (S1) ◽  
pp. 1-1
Author(s):  
C.-U. Pae
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein 70 ◽  
Odds Ratio ◽  
Gene Polymorphisms ◽  
P Value ◽  
Hsp70 Gene ◽  
Sliding Windows ◽  
Haplotypic Association ◽  
Shock Proteins ◽  
Pyrosequencing Method

Objectives:This study investigated the association between an enlarged set of SNPs at Heat shock proteins (HSPs) 70 gene and schizophrenia.Methods:Two hundred and ninety four patients with schizophrenia and 287 controls were enrolled in the study. Genotypings of 5 SNPs of HSP70 were performed using pyrosequencing method.Results:Significant association was detected at rs2075799 (allele A, Chi suare =8.03, d.f.=1 p=0.0046), but not at rs2227956 (p=0.28), rs1043618 (p=0.88), rs562047 (p=0.47) or rs539689 (p=0.32). in fact, the rs2075799*G/A genotype was more represented in patients with schizophrenia than in controls (Chi-sq=8.23, d.f.=1, p=0.0041). Haplotype based associations were also detected (global p value 0.000003); the T-A-C-C-G haplotype was more prevalent among the patients (odds ratio, OR 5.95). Sliding windows analysis revealed a major contribution from rs2227956 and rs2075799 (global-p value 0.0075), with T-A haplotype significantly associated with schizophrenia. There was no evidence of an association between the clinical variables and schizophrenia across the genotypes.Conclusion:Our results raise the possibility that HSP70 gene (i.e., haplotypes of rs2075799) might be implicated in the development of schizophrenia, although limited by rare haplotypic association with the disease. Hence further studies from different ethnics should be performed to confirm these results.

scholarly journals Acquisition of Low-temperature Tolerance in Tomatoes by Exposure to High-temperature Stress

1991 ◽  
Vol 116 (6) ◽  
pp. 1007-1012 ◽  
Author(s):  
Susan Lurie ◽  
Joshua D. Klein
Keyword(s):  
Heat Shock ◽  
Low Temperature ◽  
Tomato Fruit ◽  
Chilling Injury ◽  
High Temperature Stress ◽  
Temperature Tolerance ◽  
Phospholipid Content ◽  
Ethylene Evolution ◽  
Low Temperature Tolerance ◽  
Shock Proteins

Mature-green tomato (Lycopersicon esculentum Mill.) fruit, when kept for 3 days at 36, 38, or 40C before being kept at 2C for 3 weeks, did not develop chilling injury, while unheated fruit placed at 2C immediately after harvest did. When removed from 2 to 20C, the heated tomatoes had lower levels of K+ leakage and a higher phospholipid content than unheated fruit. Sterol levels were similar in heated and unheated fruit while malonaldehyde concentration was higher in heated fruit at transfer to 20C. The unheated tomatoes remained green, and brown areas developed under the peel; their rate of CO2 evolution was high and decreased sharply, while ethylene evolution was low and increased at 20C. In contrast, the heat-treated tomatoes ripened normally although more slowly than freshly harvested tomatoes: color developed normally, chlorophyll disappeared, and lycopene content increased, CO2, and ethylene evolution increased to a climacteric peak and K+ leakage increased with time. During prestorage heating, heat-stress ethylene production was inhibited, protein synthesis was depressed, and heat-shock proteins accumulated. There appears to be a relationship between the “heat shock response” and the protection of tomato fruit from low-temperature injury.

Single nucleotide polymorphisms in Heat Shock Protein (HSP) 90AA1 gene and their association with heat tolerance traits in Sahiwal cows

2015 ◽  
Author(s):  
Rakesh Kumar ◽  
I. D. Gupta ◽  
Archana Verma ◽  
Nishant Verma ◽  
M. R. Vineeth
Keyword(s):  
Heat Stress ◽  
Heat Shock ◽  
Heat Tolerance ◽  
Bos Taurus ◽  
Comparative Sequence Analysis ◽  
Production Performance ◽  
Nucleotide Polymorphisms ◽  
Sahiwal Cows ◽  
Shock Proteins ◽  
Hsp Genes

Heat Shock Proteins (HSPs) are group of proteins which are synthesized during heat stress. HSP genes have been reported to be associated with heat tolerance and production performance in cattle. HSP90AA1 gene has been mapped on Bos taurus autosome 21 (BTA 21) and spans nearly 5368 bp comprising 11 exons out of which first exon does not translate. The present study was carried out in Sahiwal cows (n=100) with the objectives to identify SNPs in targeted regions (exon 3, 7, 8 & 11) of HSP90AA1 gene and to analyze their association with heat tolerance traits in Sahiwal cows. Respiration rate (RR) and rectal temperature (RT) was recorded once during probable extreme hours in winter, spring and summer season. Further, heat tolerance coefficient (HTC) also calculated to see the adaptability of the animals during the period of heat stress. On the basis of comparative sequence analysis, total five SNPs were revealed at position of A1209G, A3292C, T4935C, T5218C and A5224C in the targeted region of HSP90AA1 gene. Out of these, only two SNPs at A1209G and A3292C loci were found significantly associated with heat tolerance traits in Sahiwal cows. Whereas, THI has a highly significant associated with RR, RT and HTC in all the seasons. At A1209G locus in Sahiwal cows for traits RR, genotype AA (18.40±0.46a), AG (19.60±0.85b) and GG (21.18± 0.64ab) and trait HTC AA (1.78±0.04a), AG (1.85±0.03b) and GG (1.91± 0.02c) differ significantly (p<0.01) while trait RT, AG (38.32±0.10b) and GG (38.27±0.08ab) didn’t differ significantly. For a locus A3292C for traits RR, genotype AA (21.55±1.01a), AC (19.66±1.59b) and CC (18.40±1.03c) differ significantly (p<0.05), while traits RT, genotype AA (38.41±0.12), AC (38.28±0.20) and CC (37.96±0.15) didn’t differ significantly. For trait HTC, AA (1.93±0.04a), AC (1.85±0.06b) and CC (1.79±0.05c) found significantly (p<0.05) differ with each other. Our study indicated that Sahiwal cows of AA and AC genotype had better thermo-tolerance capacity, which had been useful for genetic improvement of Sahiwal cattle for heat tolerance traits.

Performance Evaluation, Genetic Parameter and Genetic Trend Estimation of Production and Reproduction Traits of Sahiwal Cattle in Chhattisgarh

2021 ◽  
Author(s):  
Soumya Dash ◽  
Kaiser Parveen ◽  
Mohan Singh ◽  
A. Bharadwaj ◽  
K.P. Singh ◽  
...  
Keyword(s):  
Performance Evaluation ◽  
Milk Yield ◽  
Genetic Parameter ◽  
Genetic Correlations ◽  
Production Traits ◽  
Genetic Trend ◽  
Lactation Length ◽  
Reproduction Traits ◽  
Sahiwal Cows ◽  
Fixed Model

Background: Performance evaluation is necessary for formulation of suitable breeding plans for genetic improvement in a population. Therefore, this study aimed to assess the production, reproduction performances and estimate the genetic parameter and genetic trend on 649 lactation records of 238 Sahiwal cows at an organized farm in Chhattisgarh. Methods: The analysis was performed by using fixed model in LSML. Genetic trend for traits was estimated through linear regression of breeding values over the years.Result: Least-squares means for total lactation milk yield (TLMY), 305day or less milk yield (305DMY), lactation length (LL), dry period (DP) and peak yield (PY) were 1385.1±26.7 kg, 1331.4±23.6 kg, 291.0±3.7 days, 214.8±6.0 days, 7.5±0.1 kg, while for reproduction traits; age at first calving (AFC), service period (SP) and calving interval (CI), the means were 1522.8±19.1 days, 212.4±5.9 days and 498.3±6.0 days, respectively. All the traits were significantly influenced by period of calving/birth whereas season had significant effect only on PY. The highest heritability (0.29 ± 0.05) was estimated for 305DMY and lowest (0.11 ± 0.07) for SP in Sahiwal cows. Very high and positive genetic correlations was found among production traits. The first lactation 305DMY showed positive genetic trend, while LL, DP, SP and CI showed a negative genetic trend. 

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