Detection of Subclinical Ketosis in Urine of Cattle and Buffaloes

2021 ◽  
Author(s):  
Nishtha Kushwah ◽  
Mahendra Singh ◽  
Ashwani Kumar Roy
Keyword(s):  
Milk Production ◽  
Glucose Level ◽  
Milk Yield ◽  
Ketone Bodies ◽  
Calculated Data ◽  
Test Strip ◽  
Urine Samples ◽  
Subclinical Ketosis ◽  
Crossbred Cows ◽  
Sahiwal Cows

Background: Subclinical ketosis (SCK) in dairy cows is a common metabolic disorder and a strong determinant of the health and performance of cows throughout lactation. Considerable amount of milk loss takes place with SCK incidence in dairy animals. Greater benefits are to be gained by monitoring the cows for SCK. The current study was aimed to find out incidence of SCK by urine test strip (Keto-Diastix). Methods: The present investigation was carried out on crossbred and Sahiwal cows and Murrah buffaloes (n=180) of the institute herd and rural dairy farms (n=97) around Karnal. The urine samples were tested for subclinical ketosis (SCK) 5-60 days postpartum from August 2019 to March 2020. Meteriological data on wet and dry bulb temperature and ambient temperature was collected and THI was calculated. Data of SCK incidence was classified based on stages of early lactation (5-20, 21-40 and 41-60d), parity (I, II, III and above), seasons (hot-humid, autumn, winter and spring) and milk production level ( less than 10kg, 10-20 kg and greater than 20 kg/d). Milk yield and environment variables were recorded during the experiment. Urine ketone and glucose level was detected by keto-Diastix to find out incidence of SCK. Result: Mean level of ketone bodies in urine was higher (P less than 0.01) in high yielding cows as compared to the medium yielders; however it did not vary in buffaloes. Ketone bodies in urine were high (P less than 0.01) in crossbred cows than that of buffaloes and Sahiwal cows. Urine glucose level did not vary among cows and buffaloes. It is concluded that high producing crossbred cows are more vulnerable to SCK between 41-60d of lactation and in III parity and above due to peak milk production. Sahiwal cows and buffaloes suffer less from SCK probably due to medium level of milk production. Factors like high milk yield, higher parity and hot-humid stress makes the animal more vulnerable to SCK. The screening of urine samples of animals at monthly intervals may be practiced by the farmers to monitor the incidence of SCK.

Effect of Bypass Protein on Milk Production and Economic of Lactating Crossbred Cows

2020 ◽  
Vol 7 (01) ◽  
Author(s):  
RATNESH K CHOUDHARY ◽  
A Saran ROY ◽  
N K SINGH3 ◽  
SANJAY KUMAR ◽  
RAUSHAN K SINGH
Keyword(s):  
Milk Production ◽  
Milk Yield ◽  
Crossbred Cows ◽  
Lactating Cows ◽  
Feed Cost ◽  
Total Diet ◽  
T1 And T2 ◽  
Daily Milk Yield ◽  
On Farm ◽  
Daily Milk

An On-Farm Trial was conducted on 24 lactating crossbred cows for assessment of feeding formaldehyde treated mustard cake (bypass protein) on milk production and economic analysis of lactating cow. Cows were divided into three groups having 8 cows each, treatments were farmers’ practice (FP); (Control): The lactating animals under this group were fedas per the feeding schedule of the farmers (5 kg. dry roughage as rice straw + 6 hrs grazing as local grass and 4 kg. commercial concentrates), T1: The lactating animals under this group were fed as per farmers practicewith 12% mustard cake of total diet was provided to the cow by replacing the same amount of commercial concentrates andT2: The lactating animals under this group were fed as per farmers practicewith 12% formaldehyde treated mustard cake of total diet was provided to the cow by replacing same amount of commercial concentrates. The average daily milk yield of lactating cows under FP, T1 and T2 was 8.58, 8.82 and 9.85 kg per cow, respectively. Differences between FP and T2 were significant. The daily increase in milk yield was 1.27 kg and 1.03 kg in cows fed T2diet over the cows fed FP and T1diet, respectively. The B: C ratios for FP, T1 and T2 groups were 2.6, 3.0 and 3.3, respectively. The feed cost reduced in T2 group by Rs. 8.64 and increased milk production by 1.27 kg in respect to FP group.

Genotype Environment Interaction on Milk Production Traits of Crossbred Dairy Cows under Tropical Climatic Condition of India

2021 ◽  
Author(s):  
Rajalaxmi Behera ◽  
Ajoy Mandal ◽  
Saroj Rai ◽  
M. Karunakaran ◽  
Mohan Mondal ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Milk Yield ◽  
Vital Role ◽  
Genetic Group ◽  
Environment Interaction ◽  
Production Traits ◽  
Crossbred Cows ◽  
Genotype Environment Interaction ◽  
Heat Tolerant

Background: Genotype environment interaction plays vital role in animal productivity. Heat stress is one of the major environmental stressor affecting milk production and measured in terms of temperature humidity index (THI). Indian milk industry largely depends on crossbred cows bearing different degree of exotic inheritance. Thus, the role of genotype (genetic group) of the crossbred cows and environment (THI) interaction plays vital role in Indian climate which is mostly tropical in nature. Therefore, study was undertaken to examine the existence of genetic group × THI in crossbred dairy cows reared at institute herd of ICAR-National Dairy Research Institute, Eastern Regional Station, Kalyani, West Bengal. Methods: A total of 12364 records each of monthly milk yield (MMY) and average daily milk yield in a month (AMY) of crossbred cows spanned over twenty two years (1994-2015) and weather parameters(temperature and relative humidity) for the corresponding years were collected from institute records. The data were classified into 8 genetic groups according to the genetic composition and 3 THI groups (THI less than 72, THI 72-78 and THI above 78). The interaction model was used to study the G×E interaction study using least squares analysis. Result: Effect of non-genetic factors (parity, period of calving and stage of lactation) was found to be highly significant (P less than 0.01) and genetic group × THI was significant (P less than 0.05) of on both MMY and AMY. Genetic group bearing 50% Jersey and 50% Red Sindhi or Tharparkar were the most heat tolerant breeds. Jersey crossbred cows were more heat tolerant than Holstein crossbred cows. Crossbred cows with 50% Jersey inheritance performed better than higher Jersey inheritance during periods of THI above 72.

A comparison among equations to characterize lactation curves in beef cows

1992 ◽  
Vol 55 (1) ◽  
pp. 23-28 ◽  
Author(s):  
W. D. Hohenboken ◽  
A. Dudley ◽  
D. E. Moody
Keyword(s):  
Milk Production ◽  
Milk Yield ◽  
Analysis Of Variance ◽  
Beef Cows ◽  
The Other ◽  
Crossbred Cows ◽  
Lactation Curve

AbstractMonthly and fortnightly milk production records were analysed from 59 autumn-calving Angus and Angus × Holstein crossbred cows. Half the cows had been administered 10 mg thyroxine per day from day 60 to 120 of lactation and half were controls. Four published equations to characterize individual lactation curves were compared. These were: (1) log Y(n) = log –a1 + b1log n – c1n (Wood); (2) equation 1 with each log Y(n)2 weighted by Yin)2 (Wood weighted); (3) log [Y(n)/n7 = log l/a3 – k3n(Jenkins); and (4) log Y(n) = a4 – b4n‘(l + 25·5 n’) + c4n2 = d 4/ n (Morant), where Y(n) is milk yield on day n of lactation, n' is n–110 (the mid point of lactation), and the a, b, c, k and d parameters are estimated from solution of the equations. The lactation curve from the Jenkins equation projected peak milk yield to occur some 30 days later than estimates from the other equations. It underestimated production early and late in lactation and overestimated it during mid lactation. For several cows, the Morant equation projected that peak production occurred at the end of lactation. Also, analysis of variance of milk production variables was less sensitive when the traits were estimated by the Morant equation than when they were estimated by one of the others. The Wood weighted equation resulted in estimates of peak day of lactation and peak yield that were less variable and more realistic than estimates from the Wood equation. Collectively, therefore, the Wood weighted equation was deemed most suitable to characterize variability among and within these beef cows in milk production. All four equations, however, ranked the 59 cows similarly for estimated 220-day yield.

The effects of forage type and level of concentrate supplementation on food intake, diet digestibility and milk production of crossbred cows (Bos taurus × Bos indicus)

1992 ◽  
Vol 54 (2) ◽  
pp. 183-189 ◽  
Author(s):  
H. Khalili ◽  
T. Varvikko ◽  
S. Crosse
Keyword(s):  
Milk Production ◽  
Milk Yield ◽  
Milk Fat ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Daily Intake ◽  
Crossbred Cows ◽  
Concentrate Supplementation ◽  
Rumen Degradation ◽  
Forage Type

AbstractAn experiment was carried out to evaluate the effects of forage type and level of concentrate supplementation on forage intake, diet digestibility and milk production. Twenty-four crossbred cows (Bos taurus × Bos indicus), in early lactation, were allocated to the following six dietary treatments: native grass hay (H) or oat-vetch hay (OV), offered ad libitum, and supplemented with either 0, 2·5 or 5·0 kg per cow per day of a wheat-middling-based concentrate. A four-period, partially balanced, change-over design was used and the treatments were arranged as a 2 × 3 factorial.The daily dry-matter (DM) intake was 640 g (P < 0·001) higher with cows on (H) diets compared with those on OV diets. However, OV diets were more digestible (0·675 v. 0·566, P < 0·001) resulting in 800 g higher (P < 0·05) daily intake of digestible DM. The daily milk yield was 1·24 kg higher (P < 0·001) for cows on OV diets compared with H diets. Milk fat concentration was higher (P < 0·05), but milk protein concentration was lower (P < 0·001) with H diets. The rates of rumen degradation of DM and nitrogen of OV were higher than of H for all incubation periods; similarly the values of the rate constant of DM and nitrogen were greater for OV than for H.The daily DM intake increased by 3·72 kg/day (P < 0·001), when the amount of concentrate increased from 0 to 5·0 kg/day, indicating a minor substitution of basal forage by concentrate. The apparent DM digestibilities of the diets were higher when the diets were supplemented with concentrates. The milk yield increased linearly (P < 0·001), when the level of concentrate supplement increased from 0 to 5 kg, resulting in 0·52 kg more milk per day per kg additional concentrate given.

scholarly journals Effects of lactation number and different stage of lactation on milk yield of Indigenous and Crossbred cows in Bangladesh

1970 ◽  
Vol 1 (1) ◽  
pp. 31-34
Author(s):  
S Ahmad ◽  
FMA Hossain ◽  
N Islam
Keyword(s):  
Milk Production ◽  
Milk Yield ◽  
Natural Sciences ◽  
Crossbred Cows ◽  
Stage Of Lactation ◽  
Significant Difference ◽  
The Mean ◽  
Daily Milk Yield ◽  
Daily Milk

A total of 54 samples were collected from 18 milking cows from which nine were indigenous and nine were crossbred to evaluate the effects of different lactation number and different stage of lactation on the milk yield. The average daily milk yield of indigenous and crossbred cows at 1st, 2nd and 3rd lactation were 1.311, 1.633, 2.072 and 8.917, 9.567, 10.167 liter/day/cow respectively and represented the significant difference (p<0.01) within the daily milk yield of different lactations. Milk yield increased gradually from 1st to 3rd lactation. The highest milk yield was recorded in 3rd lactation and lowest was in 1st. The mean milk yield of indigenous and crossbred cows at early, middle & later stages of lactations were 1.617, 2.094, 1.306 and 9.389, 10.222, 9.039 liter/cow/day respectively that revealed stages of lactation had significant effect (p<0.01) on milk yield per day. It was observed that milk yield decreased after 2nd stage of lactation and decreased linearly up to the end of lactation. The highest milk yield was recorded in 2nd stage of lactation and lowest was in 3rd stage of lactation. The overall milk production in different lactation for indigenous cows was 1.672 liter/day and for crossbred cows was 9.550 liter/day that may be concluded as crossbred cows are superior to indigenous in concern of milk yield.Keywords: Indigenous cows; crossbred cows; lactation number; stage of lactation; milk yield.DOI: http://dx.doi.org/10.3329/ijns.v1i1.8614 International Journal of Natural Sciences (2011), 1(1):31-34 

Effect of dry cow therapy on incidence of clinical mastitis,milk yield and composition in crossbred cows

2016 ◽  
Author(s):  
Shiwani Tiwari ◽  
T. K. Mohanty ◽  
T. K. Patbandha ◽  
A. Kumaresan ◽  
M. Bhakat ◽  
...  
Keyword(s):  
Treatment Group ◽  
Milk Production ◽  
Milk Yield ◽  
Post Partum ◽  
Clinical Mastitis ◽  
Economic Losses ◽  
Control Group ◽  
Crossbred Cows ◽  
Dry Cow Therapy ◽  
The Impact

Mastitis is considered as one of the important production disease in dairy cows which incurs huge economic losses to dairy industry, despite considerable efforts has been dedicated to solve it for the last two decades. In the present study, the impact of dry cow therapy (DCT) on incidence of clinical mastitis, milk yield and composition in crossbred cows was studied. About 20 healthy crossbred cows were selected at dry-off and randomly allotted either to control or treatment group. Daily milk yield of cows was recorded up to 45 days post-partum and fortnightly separate quarter wise milk samples were collected for estimation of milk composition. While no quarter in the treatment group showed clinical mastitis, 33.33% of quarters in control group showed clinical mastitis within 45 days post-partum. Milk production increased by 9.71, 8.40 and 18.18% during first, second and third fortnight with overall value 11.84% during the entire 45 days post-calving period in DCT treated cows compared to control group. Also, the overall fat-corrected milk, solid-corrected milk and energy-corrected milk production following 45 days post-partum increased significantly (P<0.05) by 22.71, 23.70 and 22.80%, respectively in treated group than control group. However, overall mean milk components such as fat, protein, lactose, SNF and TS remained similar between treatment and control groups. Taken together, the above results indicated that DCT increased milk yield by reducing incidence of clinical mastitis during early lactation.

scholarly journals Metabolic evaluation of dairy cows submitted to three different strategies to decrease the effects of negative energy balance in early postpartum

2011 ◽  
Vol 31 (suppl 1) ◽  
pp. 11-17 ◽  
Author(s):  
Alejandra M.B García ◽  
Felipe C Cardoso ◽  
Rómulo Campos ◽  
Diego X Thedy ◽  
Félix H.D González
Keyword(s):  
Energy Balance ◽  
Fatty Liver ◽  
Dairy Cows ◽  
Milk Yield ◽  
Ketone Bodies ◽  
Negative Energy ◽  
Negative Energy Balance ◽  
Early Lactation ◽  
Blood Samples ◽  
Subclinical Ketosis

In early lactation dairy cattle suffer metabolic alterations caused by negative energy balance, which predisposes to fatty liver and ketosis. The aim of this study was to evaluate the metabolic condition of high yielding dairy cows subjected to three treatments for preventing severe lipomobilization and ketosis in early lactation. Fifty four multiparous Holstein cows yielding >30 L/day were divided into four groups: control (CN= no treatment), glucose precursor (PG= propylene-glycol), hepatic protector (Mp= Mercepton®), and energy supplement with salts of linolenic and linoleic faty acids (Mg-E= Megalac-E®). Treatments were administrated randomly at moment of calving until 8 weeks postpartum. Blood samples were collected on days 1, 7, 14, 21, 28, 35, 42 and 49 postpartum. Body condition score (BCS) was evaluated at the same periods and milk yield was recorded at 2nd, 4th, 5th, 6th, 7th, and 8th weeks of lactation. Concentrations of non-esterified fatty acids (NEFA), albumin, AST, ß-hydroxybutyrate (BHBA), cholesterol, glucose, total protein, urea and triglycerides were analyzed in blood samples. Cut-off points for subclinical ketosis were defined when BHBA >1.4 mmol/L and NEFA >0.7 mmol/L. General occurrence of subclinical ketosis was 24% during the period. An ascendant curve of cholesterol and glucose was observed from the 1st to the 8th week of lactation, while any tendency was observed with BHBA and NEFA, although differences among treatments were detected (p<0.05). BCS decreased from a mean of 3.85 at 1st week to 2.53 at 8th week of lactation (p=0.001). Milk yield was higher in the Mg-E group compared with the other treatment groups (p<0.05) Compared with the CN group, the treatments with Mp and PG did not show significant differences in blood biochemistry and milk yield. Cows receiving PG and Mg-E showed higher values of BHBA and NEFA (P<0.05), indicating accentuated lipomobilization. Supplementation with Mg-E also resulted in significant higher concentrations of cholesterol, BHBA, urea, AST and lower values of glycemia. This performance may be explained by the highest milk yield observed with this treatment. Treatments with PG and Mp did not improve milk yield, compared with control cows, but did not show metabolic evidence of ketosis, fat mobilization or fatty liver. These results suggest that treatment with Mg-E improves milk production but induces a higher negative energy balance leading to moderated lipomobilization and ketone bodies production, increasing the risk of fatty liver.

scholarly journals Production and Reproduction Performance of Sahiwal and HF × Sahiwal Cows

2021 ◽  
Author(s):  
J. Sai Prasanna ◽  
S.T. Viroji Rao ◽  
M. Gnana Prakash ◽  
Suresh Rathod ◽  
P. Kalyani ◽  
...  
Keyword(s):  
Milk Production ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Dairy Farm ◽  
Production Performance ◽  
Zebu Cattle ◽  
Economic Traits ◽  
Crossbred Cows ◽  
Lactation Length ◽  
Sahiwal Cows

Background: Milk production is one of the most important economic traits in dairy cattle. Crossing of zebu cattle (Bos indicus) with temperate cattle breeds (Bos taurus) was undertaken to improve the milk production in India. Crossbreeding has resulted in improved production performance of the dairy animlas over the past few decades. Methods: Data on 50 purebred Sahiwal cows from the Livestock Farm Complex, College of Veterinary Science, Rajendranagar, Hyderabad and 50 Holstein Friesian × Sahiwal crossbred cows maintained at the Military Dairy Farm, Secunderabad were utilized to study the influence of breed and parity on various production and reproduction traits. The various traits studied were: Total lactation milk yield (TLMY) Lactation length (LL), Peak yield (PY), Age at first service (AFS), Age at first calving (AFC), Gestation period (GP), Service period (SP), Dry period (DP) and Calving interval (Cl). Result: In the present study, significant effect of breed was found on the TLMY and PY with the crossbreds recording higher values for both the traits. The means for TLMY, PY, LL, GP, SP, DP and CI were 1768.32±109.67 kg, 10.17±0.50 kg, 304.41±13.00 days, 277.71±2.24 days, 182.60±15.51 days, 167.47±10.68 days and 430.23±13.46 days in Sahiwal and 2983.45±78.32 kg, 14.92±0.36 kg, 324.71±9.29 days, 275.34 days, 181.09±10.91 days, 127.01±7.51, 413.61±9.47 days in crossbred cows respectively. It may be concluded that the productive performance of crossbred cows was better than that of indigenous cows.

scholarly journals Subclinical Ketosis on Dairy Cows in Transition Period in Farms with Contrasting Butyric Acid Contents in Silages

2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
Fernando Vicente ◽  
María Luisa Rodríguez ◽  
Adela Martínez-Fernández ◽  
Ana Soldado ◽  
Alejandro Argamentería ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Butyric Acid ◽  
Acid Content ◽  
Transition Period ◽  
Ketone Bodies ◽  
Energy Content ◽  
Fatty Acid Analysis ◽  
Metabolizable Energy ◽  
Urine Samples ◽  
Subclinical Ketosis

This study examines the relationship between subclinical ketosis (SCK) in dairy cows and the butyric acid content of the silage used in their feeding. Twenty commercial farms were monitored over a period of 12 months. The feed at each farm and the silages used in its ration were sampled monthly for proximal analysis and for volatile fatty acid analysis. A total of 2857 urine samples were taken from 1112 cows to examine the ketonuria from about 30 days prepartum to 100 postpartum. Wide variation was recorded in the quality of silages used in the preparation of diets. Approximately 80% of the urine samples analyzed had no detectable ketone bodies, 16% returned values indicative of slight SCK, and the remainder, 4%, showed symptoms of ketosis. Most of the cases of hyperkenuria were associated with the butyric acid content of the silage used (r2=0.56;P<0.05). As the metabolizable energy content of the feed was similar, no relationship was observed between the proportion of cows with SCK and the energy content of the feed. In our study, the probability of dairy cows suffering SCK is higher when they are eating feed made from silage with a high butyric acid content (35.2 g/kg DM intake).

scholarly journals Transition period and subclinical ketosis in dairy cattle: association with milk production, metabolic and reproductive disorders and economic aspects

2020 ◽  
Vol 76 (07) ◽  
pp. 6427-2020
Author(s):  
ABDÜLKERİM DENİZ ◽  
KEMAL AKSOY ◽  
MERT METİN
Keyword(s):  
Milk Production ◽  
Dairy Cow ◽  
Energy Demand ◽  
Transition Period ◽  
Ketone Bodies ◽  
High Specificity ◽  
Health Problems ◽  
Economic Losses ◽  
Subclinical Ketosis ◽  
Specificity And Sensitivity

Many dairy cows experience a high incidence of health problems during the transition period (TP). The TP is an intermediate stage of various digestive, metabolic and reproductive functions which determine the general health status at the time of calving and during the first weeks postpartum. Negative energy balance due to increased energy demand at parturition and significantly reduced dry matter intake relative to demand is an important determinant. Consequently, substantial lipid mobilization from adipose tissue, increased oxidative stress and impaired immunity are associated with higher incidences of periparturient health problems including ketosis or subclinical ketosis (SCK), which have tremendous economic impact on dairy productivity. SCK is defined as the presence of increased blood ketone bodies (BHBA: betahydroxybutyric acid, acetone, acetoacetic acid) without clinical ketosis signs. Varying blood and milk cut-off values have been reported for BHBA concentrations defining SCK, but the most commonly accepted values are ≥ 1.2 mmol/L and ≥ 200 μmol/L respectively. This underestimated disease can impact dairy cow productivity through decreased milk production in the order of roughly 300 kg/lactation and increases the risk of metabolic and reproductive diseases such as displaced abomasum, retained placenta, metritis, mastitis, prolong oestrus interval and reduces conception rates. SCK also referred to as ‘profit robber or killer’ can cause productivity and economic losses of between $200-290 per dairy cow annually. Options for the control and prevention of SCK include controlled-release monensin capsules, and the injectable combination butaphosphan and cyanocobalamin and oral propylene glycol. SCK is easy to detect in early lactation using cow-side validated BHBA analysers with high specificity and sensitivity.

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