scholarly journals Identification of Polymorphism within Exon 8 of Bovine HSP90AA1 Gene using PCR-SSCP Technique

2021 ◽  
Vol 12 (6) ◽  
pp. 611-616
Author(s):  
J. Sai Prasanna ◽  
◽  
S. T. Viroji Rao ◽  
M. Gnana Prakash ◽  
Suresh Rathod ◽  
...  
Keyword(s):  
Reproductive Traits ◽  
Experimental Period ◽  
Single Strand Conformation Polymorphism ◽  
Crossbred Cows ◽  
Self Defense ◽  
Service Period ◽  
Reproduction Traits ◽  
Sahiwal Cows ◽  
Stressed Cells ◽  
Shock Proteins

Heat shock proteins (HSPs) are a type of molecular chaperones that aid in the recovery of stressed cells and serve as a major system for intracellular self-defense. A study was conducted during the year 2018–19 at College of Veterinary Science Rajendranagar, Hyderabad, Telengana State, India to find polymorphisms in exon 8 of the bovine HSP90AA1 gene in Sahiwal (n=50) and crossbred (n=50) cows. Blood samples were collected from the experimental animals and genomic DNA was isolated. Physiological parameters like body temperature and respiration rate for each animal were taken during the experimental period and the heat tolerance coefficient was calculated. The data on production and reproduction traits were obtained from the history sheets of the animals. To detect the polymorphism, a 539 bp fragment of the HSP90AA1 gene covering exon 8 was subjected to the Polymerase Chain Reaction-Single-Strand Conformation Polymorphism (PCR-SSCP) technique.The PCR-SSCP of exon 8 of HSP90AA1 gene yielded two genotypic patterns AA and AB corresponding to two allelic variants with frequencies of 0.85, 0.15, 0.81 and 0.19 in Sahiwal and crossbred cows, respectively. The PCR-SSCP patterns obtained were correlated with the physiological, productive, and reproductive traits in both Sahiwal and crossbred cows. The association analysis of SSCP patterns of the exon 8 of HSP90AA1 gene revealed non-significant effect in Sahiwal cows, although the AB genotype had a significantly longer service period in crossbred cows.

scholarly journals Association of SSCP Polymorphisms of HSP70 Gene with Physiological, Production and Reproduction Performance in Sahiwal and Crossbred Cows

2021 ◽  
Author(s):  
J. Sai Prasanna ◽  
S.T. Viroji Rao ◽  
M. Gnana Prakash ◽  
Suresh Rathod ◽  
P. Kalyani ◽  
...  
Keyword(s):  
Heat Shock ◽  
Milk Yield ◽  
Single Strand Conformation Polymorphism ◽  
Temperature Tolerance ◽  
Hsp70 Gene ◽  
Crossbred Cows ◽  
Reproduction Traits ◽  
Sahiwal Cows ◽  
Cellular Tolerance ◽  
Shock Proteins

Background: Cellular tolerance to heat stress is mediated by heat shock proteins (HSPs). The HSPs act as molecular chaperones and are transcribed in response to stress. Among different families of these proteins, HSP70 is considered to be related to the development of temperature tolerance. Unraveling polymorphism in heat shock protein genes could be a step towards the identification of genetic markers for selecting heat-tolerant cattle. Methods: The present study was carried out in Sahiwal (n=50) and Crossbred cows (n=50) with the objective to identify polymorphisms in HSP70 gene. Two fragments (295 and 220 bp) of HSP70 gene were subjected to Polymerase Chain Reaction-Single-Strand Conformation Polymorphism (PCR-SSCP) technique. Statistical analysis was performed to study the association of each SSCP genotype on physiological, production and reproduction traits in Sahiwal and crossbred cows using the univariate GLM model of SPSS 25. Result: The PCR-SSCP of 295 bp fragment of HSP70 gene revealed two genotypes AA and AB in Sahiwal cows and two genotypes AA and AC in crossbred cows. The association analysis revealed that genotype AA had higher peak milk yield in Sahiwal cows while the same genotype had higher total lactation milk yield, lower service period and calving interval in crossbred cows. The 220 bp fragment was found to be monomorphic in both Sahiwal and crossbred cows.

scholarly journals ​Genetic Polymorphism within Exon 3 of HSP90AA1 Gene and its Association Studies in Sahiwal and Crossbred Cows

2021 ◽  
Author(s):  
J. Sai Prasanna ◽  
S.T. Viroji Rao ◽  
M. Gnana Prakash ◽  
Suresh Rathod ◽  
P. Kalyani
Keyword(s):  
Association Studies ◽  
Critical Role ◽  
Reproductive Traits ◽  
Single Strand Conformation Polymorphism ◽  
Superior Performance ◽  
Primary System ◽  
Heat Stress Response ◽  
Crossbred Cows ◽  
Lactation Length ◽  
Climate Resilience

Background: Heat shock proteins (HSPs) are molecular chaperones that play a critical role in recovering cells from stress and form a primary system for intra cellular self defense. They are highly conserved and play a crucial role in cellular thermo tolerance and heat stress response. Though there are many HSP genes, thermo tolerance is mainly correlated with HSP70 and HSP90 genes in Livestock species. Polymorphisms in these genes have shown an association with heat tolerance, milk production, fertility and disease susceptibility in livestock. They can be used as genetic markers for the selection of animals with better climate resilience, immune response and superior performance. Methods: The present study was carried out in Sahiwal (n=50) and Crossbred cows (n=50) with the objective to identify polymorphisms in HSP90AA1 gene. A 450 bp fragment of bovine HSP90AA1 gene covering exon3 was subjected to Polymerase Chain Reaction-Single-Strand Conformation Polymorphism (PCR-SSCP) technique to identify the polymorphism. PCR-SSCP patterns were correlated with the physiological, productive and reproductive traits in Sahiwal and crossbred cows using the univariate GLM model of SPSS 25. Result: The PCR-SSCP of exon 3 of HSP90AA1 gene yielded two conformational patterns AA and AB corresponding to two allelic variants A and B in both Sahiwal and crossbred cows. The allele frequencies of A and B were 0.78 and 0.22 and 0.84 and 0.16 in Sahiwal and crossbred cows, respectively. The association analysis of SSCP patterns revealed that genotype AA had higher lactation length in Sahiwal cows and higher total lactation milk yield and peak yield in crossbred cows.

Reproductive performance of Shorthorn and Brahman crossbred cows in the dry tropics of north Queensland

1979 ◽  
Vol 19 (98) ◽  
pp. 276 ◽  
Author(s):  
RG Holroyd ◽  
PK O'Rourke ◽  
PJ Allan
Keyword(s):  
Reproductive Performance ◽  
Experimental Period ◽  
Ovarian Activity ◽  
Gestation Length ◽  
Variable Effect ◽  
Crossbred Cows ◽  
Lactating Cows ◽  
Dry Tropics ◽  
Significant Difference ◽  
Conception Rates

A study was made (1 970 to 1973) of the reproductive performance of Shorthorn and Brahman crossbred cows grazing pasture in the dry tropics of north Queensland. Breed had a variable effect on the conception rates of lactating cows, with Brahman crossbreds having significantly higher (P < 0.05) conception rates in the first year. The two breeds had similar conception rates in the following year. In the third year, overall conception rates of both breeds were reduced due to a prolonged dry season, with Shorthorn cows having significantly higher (P < 0.01) conception rates than Brahman cows. However, during this dry period, twice as many Shorthorn cows required survival feeding as did Brahman cows. Calving rates followed conception rates closely, prenatal loss in each breed being low. In all years, Shorthorns had significantly earlier (P < 0.05) calving dates than Brahmans. The interval from calving to first recorded ovarian activity was significantly longer (P < 0.05) in Brahman cross than in Shorthorn cows in only one of the three years. There was no significant difference between breeds for the interval from first recorded ovarian activity to conception, but this interval decreased with increasing age of the cow. Breed had a variable effect on gestation length. Brahman cows were generally heavier each year in April and in better condition throughout the experimental period than Shorthorn cows.

scholarly journals Molecular chaperones and heat shock proteins in atherosclerosis

2012 ◽  
Vol 302 (3) ◽  
pp. H506-H514 ◽  
Author(s):  
Qingbo Xu ◽  
Bernhard Metzler ◽  
Marjan Jahangiri ◽  
Kaushik Mandal
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Molecular Chaperones ◽  
Mammalian Cells ◽  
Vessel Wall ◽  
Protective Role ◽  
Research Field ◽  
Stressed Cells ◽  
Shock Proteins ◽  
Prevention Of Atherosclerosis

In response to stress stimuli, mammalian cells activate an ancient signaling pathway leading to the transient expression of heat shock proteins (HSPs). HSPs are a family of proteins serving as molecular chaperones that prevent the formation of nonspecific protein aggregates and assist proteins in the acquisition of their native structures. Physiologically, HSPs play a protective role in the homeostasis of the vessel wall but have an impact on immunoinflammatory processes in pathological conditions involved in the development of atherosclerosis. For instance, some members of HSPs have been shown to have immunoregulatory properties and modification of innate and adaptive response to HSPs, and can protect the vessel wall from the disease. On the other hand, a high degree of sequence homology between microbial and mammalian HSPs, due to evolutionary conservation, carries a risk of misdirected autoimmunity against HSPs expressed on the stressed cells of vascular endothelium. Furthermore, HSPs and anti-HSP antibodies have been shown to elicit production of proinflammatory cytokines. Potential therapeutic use of HSP in prevention of atherosclerosis involves achieving optimal balance between protective and immunogenic effects of HSPs and in the progress of research on vaccination. In this review, we update the progress of studies on HSPs and the integrity of the vessel wall, discuss the mechanism by which HSPs exert their role in the disease development, and highlight the potential clinic translation in the research field.

scholarly journals Changes in production and reproduction traits in population of the Slovak Spotted Cattle

2020 ◽  
Vol 23 (3) ◽  
pp. 161-166
Author(s):  
Jozef Bujko ◽  
Juraj Candrák ◽  
Július Žitný ◽  
Radovan Kasarda
Keyword(s):  
Dairy Cows ◽  
Population Size ◽  
Milk Production ◽  
Reproductive Traits ◽  
Annual Increase ◽  
Stable Level ◽  
Average Annual Increase ◽  
Positive Growth ◽  
Reproduction Traits

The study aimed to examine the analyses of the trend in the numbers of dairy cows, production and reproductive traits in the population the Slovak Spotted cattle for period 2010 to 2019. The number of Slovak Spotted dairy cows is maintained on a relatively stable level in recent years. There was a decrease in the number of animals (12 428 in 2010), but not as strong as after 1990 (85 518). From 2017 number of animals is rising from 14,062, to 14,150 (2018) and 14,627 (2019), respectively. As compared to 2010 an increase of 13.15% in 2017, 13.86% and 17.68%, in 2018 and 2019 respectively was observed. Population size raised from 113.15% (2017) to 117.68% in 2019 respectively. The average annual increase in milk production between the years 2010 to 2019 was + 157.5 kg of milk (total 1,575 kg of milk), + 5.8 kg of fat (total 58 kg of fat) and + 6.1 kg of protein (total 61 kg of protein). Positive growth of milk production in recent years is slightly comparable with the trend of breeding improvement.

scholarly journals Genetic Parameters for First Lactation and Lifetime Traits of Nili-Ravi Buffaloes

2021 ◽  
Vol 8 ◽  
Author(s):  
Pooja Tamboli ◽  
Anurag Bharadwaj ◽  
Amit Chaurasiya ◽  
Yogesh Chandrakant Bangar ◽  
Andonissamy Jerome
Keyword(s):  
Genetic Correlation ◽  
Milk Yield ◽  
Phenotypic Correlation ◽  
Productive Life ◽  
Lactation Length ◽  
Service Period ◽  
Reproduction Traits ◽  
Genetic And Phenotypic Correlations ◽  
Breeding Efficiency ◽  
Estimation Of Variance

The data on first lactation and lifetime performance records of 501 Nili-Ravi were collected for a period from 1983 to 2017 (35 years) maintained at ICAR-Central Institute for Research on Buffaloes, Sub-Campus, Nabha, Punjab. The data were analyzed to calculate heritability, genetic and phenotypic correlation for first lactation traits, viz., Age at First Calving (AFC), First Lactation Total Milk Yield (FLTMY), First Lactation Standard (305 days or less) Milk Yield (FLSMY), First Peak Milk Yield (FPY), First Lactation Length (FLL), First Dry Period (FDP), First Service Period (FSP) and First Calving Interval (FCI), Herd Life (HL), Productive Life (PL), Productive Days (PD), Unproductive Days (UD), Breeding Efficiency (BE), Total Lifetime Milk Yield (Total LTMY), Standard Lifetime Milk Yield (Standard LTMY), Milk Yield Per Day of Productive Life (MY/PL), Milk Yield Per Day of Productive Days (MY/PD), and Milk Yield Per Day of Herd Life (MY/HL). For estimation of variance component and heritability separately for each trait, the uni-trait animal model was equipped, whereas to estimate genetic and phenotypic correlations between traits, bi-trait animal models were fitted. The estimates of heritability for production and reproduction traits of Nili-Ravi were medium, i.e., 0.365 ± 0.087, 0.353 ± 0.071, 0.318 ± 0.082, 0.354 ± 0.076, and 0.362 ± 0.086 for FLSMY, FDP, FSP, FCI, and AFC, respectively. The estimates of heritability were low, i.e., 0.062 ± 0.088, 0.123 ± 0.090, 0.158 ± 0.090, 0.155 ± 0.091, and 0.129 ± 0.091 for HL, PL, PD, Total LTMY, and Standard LTMY and high, i.e., 0.669 ± 0.096 for BE. Genetic correlation for FLTMY was high with FLL (0.710 ± 0.103), and genetic correlation of FLTMY was high and positive with HL, Total LTMY, MY/PL, and MY/PD while low and positive with PL. Genetic correlation of AFC was low and negative with PL, PD, UD, BE, Total LTMY, Standard LTMY, MY/PL, and MY/PD and negative with MY/HL. Significant positive phenotypic association of FPY was seen with FLTMY, FLSMY, FLL, AFC, HL, Total LTMY, and Standard LTMY. Higher heritability of first lactation traits especially FPY suggests sufficient additive genetic variability, which can be exploited under selection and breeding policy in order to improve overall performance of Nili-Ravi buffaloes.

scholarly journals Dietary calcium levels on productive and reproductive traits of european quails

2020 ◽  
Vol 9 (1) ◽  
pp. e111911723
Author(s):  
Gabriela Queiroz Faria ◽  
Héder José D'Ávila Lima ◽  
Leonardo da Silva Costa ◽  
Jéssica Amaral Miranda ◽  
Graziela Maria Freitas Rocha ◽  
...  
Keyword(s):  
Body Weight ◽  
Reproductive Traits ◽  
Experimental Period ◽  
Dietary Calcium ◽  
Feed Consumption ◽  
Productive Performance ◽  
Coturnix Coturnix ◽  
Randomized Experimental Design ◽  
Negative Effect ◽  
Productive Traits

The objective of this study was to determine the effect of dietary calcium levels on productive and reproductive traits of European male quail (Coturnix coturnix). A total of 60 European male quail, at 35 days of age, weighing 248.9g ± 8.1g were distributed in a completely randomized experimental design with five calcium levels (1.5, 2.0, 2.5, 3.0 and 3.5%), and 12 replicates per treatment (one bird each replicate). The experimental period was 35 to 70 days of age. At 70 days of age, the parameters of male productive traits assessed were: feed consumption, calcium consumption, weight gain and body weight of birds. The reproductive traits analyzed were: hatchability and fertility of eggs. Early, intermediate and late embryonic mortality were also evaluated. The diet containing 3.16% of calcium provides higher percentage of hatched eggs and did not show a negative effect on productive performance of European quails.

Detection of Subclinical Ketosis in Urine of Cattle and Buffaloes

2021 ◽  
Author(s):  
Nishtha Kushwah ◽  
Mahendra Singh ◽  
Ashwani Kumar Roy
Keyword(s):  
Milk Production ◽  
Glucose Level ◽  
Milk Yield ◽  
Ketone Bodies ◽  
Calculated Data ◽  
Test Strip ◽  
Urine Samples ◽  
Subclinical Ketosis ◽  
Crossbred Cows ◽  
Sahiwal Cows

Background: Subclinical ketosis (SCK) in dairy cows is a common metabolic disorder and a strong determinant of the health and performance of cows throughout lactation. Considerable amount of milk loss takes place with SCK incidence in dairy animals. Greater benefits are to be gained by monitoring the cows for SCK. The current study was aimed to find out incidence of SCK by urine test strip (Keto-Diastix). Methods: The present investigation was carried out on crossbred and Sahiwal cows and Murrah buffaloes (n=180) of the institute herd and rural dairy farms (n=97) around Karnal. The urine samples were tested for subclinical ketosis (SCK) 5-60 days postpartum from August 2019 to March 2020. Meteriological data on wet and dry bulb temperature and ambient temperature was collected and THI was calculated. Data of SCK incidence was classified based on stages of early lactation (5-20, 21-40 and 41-60d), parity (I, II, III and above), seasons (hot-humid, autumn, winter and spring) and milk production level ( less than 10kg, 10-20 kg and greater than 20 kg/d). Milk yield and environment variables were recorded during the experiment. Urine ketone and glucose level was detected by keto-Diastix to find out incidence of SCK. Result: Mean level of ketone bodies in urine was higher (P less than 0.01) in high yielding cows as compared to the medium yielders; however it did not vary in buffaloes. Ketone bodies in urine were high (P less than 0.01) in crossbred cows than that of buffaloes and Sahiwal cows. Urine glucose level did not vary among cows and buffaloes. It is concluded that high producing crossbred cows are more vulnerable to SCK between 41-60d of lactation and in III parity and above due to peak milk production. Sahiwal cows and buffaloes suffer less from SCK probably due to medium level of milk production. Factors like high milk yield, higher parity and hot-humid stress makes the animal more vulnerable to SCK. The screening of urine samples of animals at monthly intervals may be practiced by the farmers to monitor the incidence of SCK.

Expression of heat shock genes of Neurospora crassa: effect of hyperthermia and other stresses on mRNA levels

1988 ◽  
Vol 66 (2) ◽  
pp. 81-92 ◽  
Author(s):  
Carol A. Curle ◽  
M. Kapoor
Keyword(s):  
Heat Shock ◽  
Neurospora Crassa ◽  
Sodium Arsenite ◽  
Gene Probe ◽  
Mrna Levels ◽  
Hsp 70 ◽  
Northern Blots ◽  
Stressed Cells ◽  
Shock Proteins

Neurospora crassa mycelium was heat shocked for intervals varying from 15–180 min. Heat shock mRNA was monitored by hybridization of Northern blots with the Drosophila hsp-70 gene probe and an inducible member of the yeast hsp-70 gene family, YG100. A 2.7 kilobase (kb) transcript, with homology to these two probes, was detected in cultures shocked for 15 min; its levels increased up to 60–90 min and declined thereafter. Sodium arsenite, too, induced the synthesis of this transcript. An additional, constitutively synthesized 2.4-kb transcript was revealed by hybridization with the yeast probe. The synthesis of this message was terminated during heat shock. Hybridization of Northern blots with the Drosophila actin gene probe demonstrated two size classes, 1.85 and 1.63 kb; the former decreased dramatically following heat shock. Recovery, as assessed by the disappearance of the 2.7-kb hsp-70-mRNA and restoration of the 1.85-kb actin message to the prestress levels, was essentially complete within 60 min of transfer to 28 °C. In vitro translations of RNA from stressed cells showed the heat shock messages to be stable and readily translatable. RNA of cells subjected to heat shock plus CdCl2 showed a higher content of messages for heat shock proteins of 70, 80, and 90 kilodaltons.

Economics of feeding protected protein and protected fat on crossbred cattle

2017 ◽  
Author(s):  
S. H. Mane ◽  
S. D. Mandakmale ◽  
C. A. Nimbalkar ◽  
D. H. Kankhare ◽  
A. T. Lokhande
Keyword(s):  
Milk Production ◽  
Block Design ◽  
Experimental Period ◽  
Crossbred Cattle ◽  
Significant Treatment ◽  
Crossbred Cows ◽  
Groundnut Cake ◽  
Randomized Block Design ◽  
Supplementation Period ◽  
Daily Milk Yield

A study was undertaken to evaluate the economics of feeding protected protein and protected fat in crossbred cows. The feeding trial of 4 months duration was conducted on 24 crossbred cattle which were subjected to treatment T0 (Control), T1 (Protected- protein), T2 ( Protected fat) and T3 (Protected protein and fat). Crossbred cows in second to fourth lactation with most probable production ability (MPPA) of average 2300 liter milk production per lactation for each group were selected. All animals were fed with 2/3 DM through roughages (2/3 from dry and 1/3 from green) + 1/3 DM from concentrate mixture. In T0 and T2 groups untreated groundnut cake was given in ration. Whereas, in T1 and T3 groups, groundnut cake was treated with formaldehyde (FA) (@ 1.0 gm FA /100g CP). Also bypass fat (99%) was supplemented in T2 and T3 groups @ 10 gm per liter milk production. Daily DM intake and daily milk yield for individual animals were recorded. The data generated during experimental period were analyzed by Factorial Randomized Block Design (FRBD) with SAS, 9.3 versions.The DMI was significantly (P>0.05) higher in T3 (12.72) followed by T1 (12.59), T0 (12.47) and T2 (12.23). However, the DMI/ 100 kg body weight was 3.05, 3.01, 3.13 and 2.81 kg/d in T0, T1, T2 and T3, respectively with non significant treatment. The average milk production during supplementation period was 9.82, 11.76, 11.41 and 12.43 kg/d in group T0, T1, T2 and T3, respectively which was significantly 16.49% higher in T1, 13.93% higher in T2 and 20.99% in T3 over T0. The net return over feed cost was highest in T3 group followed by T1 and T2 over T0.

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