A correlation study of Boar taint-related genes, Boar tant substances and sex hormones in Bama miniature pigs at defferent ages

2015 ◽  
Author(s):  
Q. H. Wang ◽  
J. Y. Chen ◽  
K. N. Guo ◽  
X. B. Zheng ◽  
Q. Wu ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Sex Hormones ◽  
Correlation Study ◽  
Boar Taint ◽  
Miniature Pig ◽  
Miniature Pigs ◽  
Meat Flavor ◽  
The Relationship ◽  
Sus Scrofa Domestica

The Bama miniature pig (Sus scrofa domestica), endemic in China,which is characterized by its small size and specific meat flavor. The evaluation of pork quality is closely related to boar taint. However, there are no published studies of boar taint in Bama miniature pigs. In this study, the correlation between mRNA expression of boar taint-related genes,boar taint substances (androstenone and skatole) and sex hormones (testosterone, estradiol) of Bama miniature pigs at consecutive ages were examined. We found a gradual increase of mRNA (CYP2E1, CYP2A19, CYP2C34, and COUP-TF1) expression in the livers of pigs aged from birth to 6 months of age. The mRNA expression of COUP-TF1 and CYP2C34 peaked at 12 months old. However, the mRNA expression of CYP2E1 and CYP2A19 declined after 6 months age. The mRNA expression of CYP11A1, CYP17A1, StAR, and COUP-TF1 was stable over time in the testicle of Bama miniature pig. However, a substantial increase in their relative expression levels was observed before sexual maturity. Boar age was positively correlated to sex hormone (testosterone, estradiol) and boar taint substances (androstenone, skatole). There is in vivo gene regulation in boars that controls the relationship between boar taint substances and sex hormones. In addition, CYP11A1, CYP17A1, StAR, and sex hormones (testosterone, estradiol) can be used as markers for low boar taint study. Our study elucidated the correlation of boar taint-related genes,boar taint substances and sex hormones. These results have provided reference for applied research using Bama miniature pigs as a model for livestock production.

scholarly journals Comparison of the Resistance to Bending Forces of the 4.5 LCP Plate-rod Construct and of 4.5 LCP Alone Applied to Segmental Femoral Defects in Miniature Pigs

2010 ◽  
Vol 79 (4) ◽  
pp. 613-620 ◽  
Author(s):  
Lucie Urbanová ◽  
Robert Srnec ◽  
Pavel Proks ◽  
Ladislav Stehlík ◽  
Zdeněk Florian ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Femoral Diaphysis ◽  
Miniature Pig ◽  
Femoral Bone ◽  
Bone Implant ◽  
Miniature Pigs ◽  
Bone Defect Healing ◽  
Considerable Strain

The study deals with the determination of mechanical properties, namely resistance to bending forces, of flexible buttress osteosynthesis using two different bone-implant constructs stabilizing experimental segmental femoral bone defects (segmental ostectomy) in a miniature pig ex vivo model using 4.5 mm titanium LCP and a 3 mm intramedullary pin (“plate and rod” construct) (PR-LCP), versus the 4.5 mm titanium LCP alone (A-LCP). The “plate and rod” fixation (PR-LCP) of the segmental femoral defect is significantly more resistant (p < 0.05) to bending forces (200 N, 300 N, and 500 N) than LCP alone (A-LCP). Stabilisation of experimental segmental lesions of the femoral diaphysis in miniature pigs by flexible bridging osteosynthesis 4.5 mm LCP in combination with the “plate and rod” construct appears to be a suitable fixation of non-reducible fractures where considerable strain of the implants by bending forces can be assumed. These findings will be used in upcoming in vivo experiments in the miniature pig to investigate bone defect healing after transplantation of mesenchymal stem cells in combination with biocompatible scaffolds.

scholarly journals Evaluation of the relationship between the 14-3-3ε protein and LvRab11 in the shrimp Litopenaeus vannamei during WSSV infection

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Guson Boonyoung ◽  
Tanate Panrat ◽  
Amornrat Phongdara ◽  
Warapond Wanna
Keyword(s):  
Mrna Expression ◽  
Litopenaeus Vannamei ◽  
Early Stage ◽  
In Silico Analysis ◽  
Neutralization Assay ◽  
White Shrimp ◽  
Reading Frame ◽  
Infection Processes ◽  
The Relationship

AbstractThe 14-3-3 proteins interact with a wide variety of cellular proteins for many diverse functions in biological processes. In this study, a yeast two-hybrid assay revealed that two 14-3-3ε isoforms (14-3-3ES and 14-3-3EL) interacted with Rab11 in the white shrimp Litopenaeus vannamei (LvRab11). The interaction of 14-3-3ε and LvRab11 was confirmed by a GST pull-down assay. The LvRab11 open reading frame was 645 bp long, encoding a protein of 214 amino acids. Possible complexes of 14-3-3ε isoforms and LvRab11 were elucidated by in silico analysis, in which LvRab11 showed a better binding energy score with 14-3-3EL than with 14-3-3ES. In shrimp challenged with the white spot syndrome virus (WSSV), the mRNA expression levels of LvRab11 and 14-3-3ε were significantly upregulated at 48 h after challenge. To determine whether LvRab11 and binding between 14-3-3ε and LvRab11 are active against WSSV infection, an in vivo neutralization assay and RNA interference were performed. The results of in vivo neutralization showed that LvRab11 and complexes of 14-3-3ε/LvRab11 delayed mortality in shrimp challenged with WSSV. Interestingly, in the RNAi experiments, the silencing effect of LvRab11 in WSSV-infected shrimp resulted in decreased ie-1 mRNA expression and WSSV copy number. Whereas suppression of complex 14-3-3ε/LvRab11 increased WSSV replication. This study has suggested two functions of LvRab11 in shrimp innate immunity; (1) at the early stage of WSSV infection, LvRab11 might play an important role in WSSV infection processes and (2) at the late stage of infection, the 14-3-3ε/LvRab11 interaction acquires functions that are involved in immune response against WSSV invasion.

Semen quality and fertility of Bama miniature pig (Sus scrofa domestica)

2015 ◽  
Author(s):  
Shibin Zhang ◽  
Kuonan Guo ◽  
Deying Kong ◽  
Yu Liu ◽  
Haitao Shang ◽  
...  
Keyword(s):  
Male Fertility ◽  
Sus Scrofa ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Miniature Pig ◽  
Miniature Pigs ◽  
Abnormal Sperm ◽  
Study Results ◽  
Sus Scrofa Domestica ◽  
Farrowing Rate

Semen quality and fertility of Bama miniature pig and the effect of season on these parameters were determined in this study. Results were as follows: the overall mean ejaculate volume, 55.71 ± 15.21 ml; sperm concentration, 0.66 ± 0.22 × 108/ml; sperm motility, 65.39% ± 16.45%; abnormal sperm percentage, 12.16% ± 7.70%; farrowing rate, 76.44%; litter size, 7.74 ± 2.46. It showed that the semen quality and fertility of Bama miniature pigs were lower than the normal level of the larger commercial and local breeds. In terms of the effect of season, we noted that the semen quality and fertility of Bama miniature pigs were not worst in summer. This may indicate that Bama miniature pig is a relative heat-tolerant breed in comparison to larger commercial and local breeds. These findings in this study could provide reference material for improve management and husbandry in practice and for using Bama miniature pigs as animal model especially in male fertility studies.

Effects of ovariectomy on energy metabolism in exercising rat muscle studied by 31P-NMR

1989 ◽  
Vol 67 (5) ◽  
pp. 2060-2065 ◽  
Author(s):  
Z. Roth ◽  
Z. Argov ◽  
J. Maris ◽  
K. K. McCully ◽  
J. S. Leigh ◽  
...  
Keyword(s):  
Sex Hormones ◽  
Female Athletes ◽  
High Energy ◽  
Time Integral ◽  
Menopausal Women ◽  
Tension Time ◽  
Postexercise Recovery ◽  
Sciatic Nerve Stimulation ◽  
The Relationship

The effects of ovariectomy on metabolism of high-energy phosphate compounds during and after exercise were studied in hindleg muscles of 14 rats. Sciatic nerve stimulation was used to establish different work loads, and the changes in inorganic phosphate-to-phosphocreatine ratios (Pi/PCr) were recorded by 31P nuclear magnetic resonance (NMR) in vivo. Four weeks after ovariectomy, there was evidence of significantly higher Pi/PCr during work at stimulation rates greater than 0.5 Hz. The slope for the stimulation rate-to-Pi/PCr relationship decreased from 1.98 +/- 0.15 to 1.36 +/- 0.2 Hz/Pi/PCr after ovariectomy. The normalized tension output of these muscles, tested separately using identical stimulation protocols, was not changed with ovariectomy. Thus the relationship between work (tension-time integral) and bioenergetic cost (Pi/PCr) suggested reduced maximal enzyme activity (Vmax) by 9-17% as a result of lack of ovarian sex hormones, but no change in Michaelis-Menten constant (Km) was found. Postexercise recovery was also significantly slower (3.27 +/- 0.54 PCr/Pi units per minute compared with 4.04 +/- 1.08 in controls). It is suggested that reduced levels of ovarian sex hormones decrease oxidative phosphorylation. Cytochrome oxidase activity was reduced in these muscles by 40%, but other mitochondrial enzyme systems may be affected as well. The possible significance of these data is the implication of a reduced capacity for menopausal women or amenorrheic female athletes to perform prolonged intensive exercise.

scholarly journals Does alternation of Candida albicans TUP1 gene expression affect the progress of symptomatic recurrent vulvovaginal candidiasis?

2020 ◽  
Author(s):  
Mona Ghazanfari ◽  
Azam Fattahi ◽  
Mehraban Falahati ◽  
Majid Bakhshizadeh ◽  
Maryam Roudbary ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mrna Expression ◽  
Expression Patterns ◽  
Vulvovaginal Candidiasis ◽  
In Vivo Studies ◽  
Recurrent Vulvovaginal Candidiasis ◽  
Qrt Pcr ◽  
Asymptomatic Patients ◽  
The Relationship

Background and Purpose: Recurrent vulvovaginal candidiasis (RVVC) is one of the most common gynecological conditions in healthy and diabetic women, as well as antibiotic users. The present study was conducted to determine the relationship between TUP1 gene expression patterns and symptomatic recurrent C. albicans infections. Materials and Methods: This research was performed on C. albicans samples isolated from the vaginal specimens obtained from 31 individuals with RVVC in 2016. The reference strain C. albicans ATCC 10231, 10 C. albicans strains isolated from minimally symptomatic patients, and 10 isolates from asymptomatic patients were also used as control strains. The relative mRNA expression of the TUP1 gene was quantified using quantitative real-time polymerase chain reaction (QRT-PCR). Results: The QRT-PCR results revealed that TUP1 mRNA expression was significantly decreased (0.001-0.930 fold) in the C. albicans isolates obtained from RVVC patients (P<0.001). However, no TUP1 expression was detectable in the isolates collected from asymptomatic patients. The results also indicated a significant correlation between TUP1 mRNA expression level and the severity of itching and discharge (P<0.001). Conclusion: The present results were suggestive of the probable contribution of TUP1, as a part of the transcriptional repressor, to the severity of the symptoms related to C. albicans infections in the vagina. Regarding this, it is required to perform more in vivo studies using a larger sample size to characterize the regulatory or stimulatory function of TUP1 in the severity of RVVC symptoms. Furthermore, the study and identification of the genes involved in the severity of the symptomatic manifestations of C. albicans, especially in resistant strains, may lead to the recognition of an alternative antifungal target to enable the development of an effective agent.

scholarly journals Apoptotic Molecules In Chronic Leukemia

2016 ◽  
Vol 15 (4) ◽  
pp. 651-654
Author(s):  
Faris Q Alenzi ◽  
Amani A Ballow ◽  
Meaad Q Alanazi ◽  
Fahad G Alanazi ◽  
Omar Bagader ◽  
...  
Keyword(s):  
Cell Death ◽  
Mrna Expression ◽  
Blast Crisis ◽  
Quantitative Polymerase Chain Reaction ◽  
Medical Science ◽  
Myeloid Cell ◽  
Messenger Ribonucleic Acid ◽  
Related Proteins ◽  
The Relationship

Background: Chronic myeloid leukemia (CML) is characterized by the spread of malignant cells that exhibit resistance to caspase-mediated cell death (apoptosis) and this mechanism is proposed to play an important role in myeloid cell growth. However, the extent to which caspase-mediated cell death plays a crucial role in the regulation of myelopoiesis remains controversial.Objectives & Proceedure: The objectives of this study were to examine whether or not caspase-mediated cell death-related proteins take part in the development of CML and to also to detect the relationship between Fas, p53 and caspase-mediated cell death protease activating factor (Apaf-1) in 5 patients with CML using the real-time quantitative polymerase chain reaction. We demonstratedthatp53 and Apaf-1 messenger ribonucleic acid (mRNA) expression was moderately elevated (up to 5 fold, p<0.05) in 4 out of 5 CML patients. One patient with a p53 point mutation, exhibited a far greater elevation of p53 mRNA expression throughout their blast crisis, but in contrast, displayed a significant reduction in levels of Apaf-1 mRNA and Fas mRNA.Conclusion: Our results show in-vivo linkages between Fas, p53 and Apaf-1 transcription parameters suggesting that the key genes involved in the caspase-mediated cell death might contribute to CML disease development.Bangladesh Journal of Medical Science Vol.15(4) 2016 p.651-654

55 DIFFERENTIAL TRANSCRIPTIONAL GENE EXPRESSION BETWEEN MINIATURE AND LANDRACE PIG FETUSES: IMPLICATION FOR PRODUCTION OF CLONED MINIATURE PIGS USING LANDRACE PIGS AS OOCYTE DONORS AND SURROGATE MOTHERS

2007 ◽  
Vol 19 (1) ◽  
pp. 145
Author(s):  
O. J. Koo ◽  
S. H. Lee ◽  
M. S. Hossein ◽  
S. K. Kang ◽  
D. Y. Kim ◽  
...  
Keyword(s):  
Expression Patterns ◽  
Developmental Competence ◽  
Miniature Pig ◽  
Surrogate Mothers ◽  
Miniature Pigs ◽  
Significant Difference ◽  
Oocyte Donors ◽  
Landrace Pig

Miniature pigs are regarded as a better organ donor breed for xenotransplantation because of their compatible organ size with human than any other pig breeds. The present study was performed to evaluate a somatic cell nuclear transfer (SCNT) system for producing cloned miniature pigs using Landrace pigs as oocyte donors and surrogate mothers. In Experiment 1, differential mRNA expression patterns of Day 30 gestation fetuses between miniature and Landrace breeds were compared using 13 610 cDNA microarray (based on Pig Genome Oligo sets; Qiagen, Valencia, CA, USA). In each breed, total mRNA from 3 fetuses was pooled before hybridization to minimize individual sample effect. With the fold-change test, 1551 cDNAs (11.40% of total) showed more than a 2-fold difference of intensity between the 2 breeds. In miniature fetuses, 252 genes were up-regulated and 1299 were down-regulated compared to Landrace ones. Among them some crucial genes related to implantation, including vascular endothelial growth factor (VEGF), vitronectin, and c-kit, were significantly down-regulated in miniature pig fetuses. In Experiment 2, in vitro developmental competence of SCNT embryos using fibroblasts from both breeds as nuclei donors were evaluated. In total, 352 miniature and 345 Landrace cloned embryos were cultured in vitro. There was no significant difference in fusion rate (78.78 vs. 77.48%), cleavage rate (69.8 vs. 65.3%), blastocyst rate (15.5 vs. 16.7%), and total cell number of blastocysts (48.0 &plusmn; 11.2 vs. 51.9 &plusmn; 17.5; all respectively). In Experiment 3, in vivo development was also monitored. In total, 1684 and 1354 SCNT embryos derived from miniature and Landrace pigs were transferred to 9 and 7 Landrace pig surrogate mothers, respectively. Overall, miniature embryos showed less in vivo developmental potency than Landrace ones; pregnancy rate at Day 30 of gestation (44 vs. 86%) and birth rate (11 vs. 43%) were low in miniature pig (based on the number of surrogates). Mean efficiency of SCNT embryo to term (0.24 vs. 1.55%) and mean litter size (4 vs. 7) were also low in miniature pigs. These results suggest that although in vitro development of SCNT embryos using recipient oocytes from Landrace pigs was similar between the 2 breeds, miniature pig embryos cannot interact with Landrace pig's reproductive tract properly and fail to implant, thus inhibiting fetal growth. In conclusion, cloned miniature pigs can be successfully produced using Landrace pigs as oocyte donors and surrogate mothers; however, the efficiency was very low due to transcriptional differences of fetuses between the 2 breeds. The authors are grateful for a graduate fellowship provided by the Ministry of Education, through the BK21 program.

Ultrastructural Correlations between Clonal Human Tumor Colonies and in Vivo Neoplasms

1982 ◽  
Vol 40 ◽  
pp. 210-211
Author(s):  
M.J. Murphy ◽  
R.R. Price ◽  
J.C. Sloman
Keyword(s):  
Cultured Cells ◽  
Human Tumor ◽  
Cell Type ◽  
Tumor Mass ◽  
Initial Cell ◽  
Cloning Assay ◽  
Human Tumor Cloning ◽  
The Relationship

The in vitro human tumor cloning assay originally described by Salmon and Hamburger has been applied recently to the investigation of differential anti-tumor drug sensitivities over a broad range of human neoplasms. A major problem in the acceptance of this technique has been the question of the relationship between the cultured cells and the original patient tumor, i.e., whether the colonies that develop derive from the neoplasm or from some other cell type within the initial cell population. A study of the ultrastructural morphology of the cultured cells vs. patient tumor has therefore been undertaken to resolve this question. Direct correlation was assured by division of a common tumor mass at surgical resection, one biopsy being fixed for TEM studies, the second being rapidly transported to the laboratory for culture.

Do Extra-Platelet Sources Contribute to the Plasma Level of Thrombospondin?

1988 ◽  
Vol 59 (02) ◽  
pp. 273-276 ◽  
Author(s):  
J Dawes ◽  
D A Pratt ◽  
M S Dewar ◽  
F E Preston
Keyword(s):  
Platelet Count ◽  
Background Concentration ◽  
Serum Concentrations ◽  
Bone Marrow Depression ◽  
Serial Sampling ◽  
Control Serum ◽  
Platelet Release ◽  
Highly Correlated ◽  
The Relationship

SummaryThrombospondin, a trimeric glycoprotein contained in the platelet α-granules, has been proposed as a marker of in vivo platelet activation. However, it is also synthesised by a range of other cells. The extraplatelet contribution to plasma levels of thrombospondin was therefore estimated by investigating the relationship between plasma thrombospondin levels and platelet count in samples from profoundly thrombocytopenic patients with marrow hypoplasia, using the platelet-specific α-granule protein β-thromboglobulin as control. Serum concentrations of both proteins were highly correlated with platelet count, but while plasma β-thromboglobulin levels and platelet count also correlated, there was no relationship between the number of platelets and thrombospondin concentrations in plasma. Serial sampling of patients recovering from bone marrow depression indicated that the plasma thrombospondin contributed by platelets is superimposed on a background concentration of at least 50 ng/ml probably derived from a non-platelet source, and plasma thrombospondin levels do not simply reflect platelet release.

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