Gross and Histopathological Findings of Naturally Occurring Anaplasma marginale Infection in Cattle

2021 ◽  
Author(s):  
Dhruba Das ◽  
Kalyan Sarma ◽  
Parimal Roychoudhury ◽  
G.E. Chethan ◽  
R. Ravindran ◽  
...  
Keyword(s):  
Histopathological Examination ◽  
Anaplasma Marginale ◽  
High Fever ◽  
Sequencing Analysis ◽  
Post Mortem ◽  
Histopathological Changes ◽  
Haemolytic Disease ◽  
Post Mortem Examination ◽  
Naturally Occurring ◽  
Bovine Anaplasmosis

Background: Bovine anaplasmosis is a haemolytic disease of cattle caused by an obligate intra-erythrocytic bacterium Anaplasma marginale and is characterized by high fever, dyspnoea, anaemia, icterus, decreased milk production and death. The present study was undertaken to study the gross and histopathological changes caused by naturally occurring A. marginale infection in cattle. Methods: History and clinical examination of animals revealed inappetance/anorexia, presence of tick infestation, high fever, pale or icteric mucous membranes, tachycardia, dyspnoea and lymphadenomegaly. The diagnosis of A. marginale infection was done by blood smear examination and further confirmed by polymerase chain reaction and sequencing analysis. During the present study, two animals did not respond to treatment and were succumbed to A. marginale infection. A systematic post-mortem examination was performed on the animals and gross lesions were recorded. The tissue samples from various vital organs such as heart, lungs, liver, spleen and kidneys were collected in 10% neutral buffered formalin and processed for histopathological examination. Result: The post-mortem examination revealed emaciated carcass with yellowish discoloration of serosal surfaces of the abdominal organs, haemorrhagic heart, congestion of lungs, hepatomegaly, distension of the gallbladder and splenomegaly. The major histopathological changes noticed included myocardial degeneration and necrosis, interstitial pneumonic changes, degenerative changes in hepatocytes and renal tubular epithelial cells, enlargement of red pulp area of spleen with histiocytic proliferation and hemosiderosis. Histopathology also revealed inflammatory process characterized by infiltration of mononuclear cells in various organs. Very few reports are available on the pathological aspects of A. marginale infection and thus the outcome of the present study can yield valuable information to aid in the diagnosis of bovine anaplasmosis under field conditions.

scholarly journals Hematoxylin - Eosin-Methylene Blue Staining in a Dog Hepatic Amyloidosis Case

2017 ◽  
Vol 54 (3) ◽  
pp. 546-548
Author(s):  
Adrian Stancu ◽  
Liliana Carpinisan ◽  
Alina Ghise ◽  
Marius Pentea ◽  
Delia Mira Berceanu Vaduva ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Histopathological Examination ◽  
Blue Staining ◽  
Microscopical Examination ◽  
Post Mortem ◽  
Histopathological Changes ◽  
Post Mortem Examination ◽  
Hepatic Amyloidosis ◽  
Hematoxylin Eosin

A post-mortem examination of an 1,5 years old Shar- Peis, dog was performed in order to find out the death cause. The macroscopically examination revealed a large amount of blood in the abdominal (hemoperitoneum) The liver was sampled for histopathological examination. Hematoxylin-eosin-methylene blue staining wasperformed in order to prepare the samples for microscopical examination. Based on the specific histopathological changes and taking into account all the pathological data, the diagnosis was liver amiloidosis.

scholarly journals Knowledge and Attitude of 3rd Year Medical Students regarding Post Mortem Examination

2016 ◽  
Vol 12 (2) ◽  
pp. 9-14
Author(s):  
Mushtaq Ahmad ◽  
Md Fashiur Rahman ◽  
Farial Naima Rahman ◽  
Md Afzalur Rahman
Keyword(s):  
Medical Students ◽  
Chemical Analysis ◽  
Cause Of Death ◽  
Histopathological Examination ◽  
Armed Forces ◽  
Post Mortem ◽  
Cross Sectional ◽  
Post Mortem Examination ◽  
Medical College ◽  
Knowledge And Attitude

Introduction: Post mortem examination is a process which is required to establish the exact cause of death in sudden, suspicious or unnatural cases. It determines the mode, manner and time passed since death. Objective: To explore the knowledge and attitude of 3rd year medical students regarding post mortem examination at the very beginning of their para clinical course study. Materials and Methods: This descriptive cross sectional study was performed at Armed Forces Medical College, Dhaka during the period of July to September 2016. One hundred fifteen 3rd year students were selected by purposive and convenient method and asked to respond anonymously to a 18 item selfadministered, predesigned, pretested multiple choice questionnaire which dealt with their views on autopsy practice, the knowledge procedure, attitude and perception towards post mortem examination. Ethical permission of authority concerned and verbal consent was obtained from every respondent students. Results: In this study, 108(93.91 %) students gave positive answer about the requirement of autopsy in unnatural death cases. A large number of students 57(49.56%) did not know about the types of autopsy, but more than half 67(58.26%) knew about virtual autopsy. Seventy two (62.61%) answered that heart is the organ sent for histopathological examination and majority 71(61.74%) could identify the viscera sent for chemical analysis. But most of them 65(56.52%) had wrong idea about the laboratory for chemical analysis. Also 70(60.87%) thought that formalin is used for preservation of viscera. Majority students 88(76.52%) answered that autopsy is performed to detect the cause of death. Greater part of respondent 98(85.22%) also said postmortem examination is neither harassment to victim’s relatives and nor disrespect to a dead body. Most of the students 113(98.26%) answered that observation of post mortem examination should be part of MBBS course curriculum and all 115(100%) gave positive response about utility of this chapter in their course studies. Forty one (35.65%) came to know about autopsy from medical books, followed by 35(30.44%) from newspaper/ story books. Almost all 113(98.26%) did not watch any autopsy before their admission to medical college. A greater part of students 74(64.35%) did not know whether autopsy cause any disfigurement of dead bodies. In present study 102(88.69%) students showed positive attitude to have post mortem examination of relatives if required. Majority of samples thought post mortem is not required in putrefied body and 93(80.87%) answered that body can be preserved at mortuary cooler after post mortem when required. Conclusion: Post mortem is an integral part of Forensic Medicine course curriculum. Teaching professionals should give more emphasis to make this chapter more interesting to the medical students. Journal of Armed Forces Medical College Bangladesh Vol.12(2) 2016: 9-14

Ocular pathological changes in hamsters experimentally infected withSchistosoma mansoni

2016 ◽  
Vol 90 (6) ◽  
pp. 773-778 ◽  
Author(s):  
H.I.H. Ismail ◽  
D.S. Ashour ◽  
D.M. Abou Rayia ◽  
A.L. Ali
Keyword(s):  
Histopathological Examination ◽  
Experimental Period ◽  
Post Mortem ◽  
Pathological Changes ◽  
Histopathological Changes ◽  
Polypoid Growth ◽  
Ocular Lesions ◽  
Ocular Changes ◽  
Immunohistochemical Studies ◽  
Suspicious Lesions

AbstractOcular lesions have been reported in patients with schistosomiasis; however, the problem with studying schistosomal infection of the human eye is that biopsies are almost impossible to take, and histopathological examination of suspicious lesions can only be undertaken post-mortem or after enucleation. This work aimed to study the possible effects and pathogenesis of schistosomiasis on the eye. This study involved 55 hamsters; five hamsters remained non-infected and the remaining 50 hamsters were infected withSchistosoma mansonicercariae. Infected hamsters were sacrificed on weeks 8, 12, 16 and 20 post-infection (pi). Eye sections were prepared and stained for histopathological and immunohistochemical studies. Histopathological changes detected in hamsters infected after 16 and 20 weeks included looseness and oedema of the innermost retinal layers together with hyperplastic polypoid growth. Neither eggs nor granulomata were detected in eye sections throughout the experimental period. Deposition ofS. mansoniantigen was revealed in 35% of infected hamsters. Later, on weeks 16 and 20 pi, moderate subepithelial conjuctival deposits and marked subchoroidal and scleral deposition were detected. In conclusion, the deposition of schistosomal antigen and immune complexes may play a pivotal role in the ocular changes that occur in schistosomiasis, even in the absence of detectableSchistosomaeggs. Schistosomiasis should be suspected in cases with unexplained ophthalmological findings, especially in endemic areas.

scholarly journals Spontaneous outbreak of Pascalia glauca poisoning in sheep in Argentina

2021 ◽  
Vol 14 (2) ◽  
pp. 99-101
Author(s):  
Matías Liboreiro ◽  
◽  
Carlos Flores Olivares ◽  
Joaquín Armendano ◽  
Carlos Schild ◽  
...  
Keyword(s):  
Histopathological Examination ◽  
Hepatic Necrosis ◽  
Nasal Discharge ◽  
Post Mortem ◽  
Pathological Findings ◽  
Post Mortem Examination ◽  
Reticular Pattern ◽  
Commercial Flock ◽  
Abdominal Breathing ◽  
Toxic Plant

Pascalia glauca is a native weed and one of the most common hepatotoxic plant affecting cattle in Argentina. Although experimental P. glauca poisoning have been reported in sheep, no spontaneous cases have been reported in this species. This work describes an outbreak of intoxication after spontaneous consumption of P. glauca, affecting 20% (6/30) sheep of a commercial flock. Affected sheep were ataxic, depressive, with mucous nasal discharge, cough and abdominal breathing. During post mortem examination, liver was swollen and a diffuse enhancement of the reticular pattern (“nutmeg liver”) was visible. Histopathological examination reveals severe and extensive acute diffuse centrilobular hepatic necrosis with hemorrhage. The presence of the toxic plant, the clinical and pathological findings allows us to confirm the etiology of this outbreak.

Contribution Regarding the Use of Immunohistochimical Examination in Hemangiosarcoma

2020 ◽  
Vol 71 (8) ◽  
pp. 323-326
Author(s):  
Adrian Stancu ◽  
Romeo Teodor Cristina ◽  
Alina Ghise ◽  
Marius Pentea ◽  
Sorin Pasca ◽  
...  
Keyword(s):  
Cause Of Death ◽  
Right Atrium ◽  
Histopathological Examination ◽  
Abdominal Cavity ◽  
Post Mortem ◽  
Post Mortem Examination ◽  
The Right ◽  
Macroscopic Examination

A post-mortem examination of a 5-year-old female Labrador sex female was performed to find out the cause of death. The macroscopic examination revealed a large amount of blood in the abdominal cavity (hemoperitoneum) and pericardial (hemopericard) and also the rupture of the right atrium. The spleen was taken for histopathological examination.

scholarly journals Oak leaf (Quercus spp.) intoxication in a sheep

2021 ◽  
Vol 90 (3) ◽  
pp. 133-137
Author(s):  
S. De Backer ◽  
K. Chiers ◽  
L. Van Brantegem
Keyword(s):  
Differential Diagnosis ◽  
Case Report ◽  
Histopathological Examination ◽  
Post Mortem ◽  
Post Mortem Examination ◽  
Lateral Decubitus ◽  
Quercus Spp ◽  
Oak Leaves

A twenty-year-old ewe presented with acute lateral decubitus, tremor and dyspnea, followed by death. Post-mortem examination revealed a large amount of oak leaves in the rumen, reticulum and omasum. The duodenum had a focal hemorrhagic content and multifocal hemorrhages were present in several organs. Histopathological examination of the kidneys showed characteristic lesions of oak intoxication, such as intratubular hemorrhage, tubular degeneration and necrosis. This case report illustrates the importance to include oak intoxication in the differential diagnosis of acute death in sheep and the value of post-mortem and histopathological examination.

TO FIND OUT ELECTRO-CARDIOGRAPHIC CHANGES & ECHO CHANGES IN ALUMINIUM PHOSPHIDE AND ETHYLENE DIBROMIDE POISONING

2020 ◽  
Vol 4 (10) ◽  
Author(s):  
Hemant Nargawe ◽  
Sumeet Sisodiya
Keyword(s):  
Biochemical Analysis ◽  
Histopathological Examination ◽  
Common Finding ◽  
Histopathological Changes ◽  
Ethylene Dibromide ◽  
Medical College ◽  
Aluminium Phosphide ◽  
Urine Examination ◽  
Cardiovascular Involvement ◽  
Careful Clinical Examination

Background & Method: The study was conducted in the Department of Medicine Shyam Shah Medical College and Associated Sanjay Gandhi Memorial Hospital, Rewa (M.P). History was followed by a careful clinical examination i.e. cardiovascular, respiratory and gastrointestinal and nervous system. Investigations had done included routine haematological examination, Biochemical analysis, urine examination, ECG, 2 D. Echo & Histopathological examination was done. Result: ST-T changes were most common finding in Aluminium phosphide poisoning in relation to mortality. However hyperkalemia was the most ominous finding associated with 100% mortality, ECG finding in EDB was normal ECG. The most ominous finding was arrhythmia which was associated with 100% mortality. Survivors of ethylene dibromide poisoning echocardiography was normal in 11 (84.61%) followed by pericardial effusion in 2 (15.38%) patients. Conclusion: Noteworthy finding was absence of correlation between cardiovascular involvement, histopathological changes and ECG findings. It was seen that even if ECG showed normal pattern there were significant histopathological changes in heart. Keywords: electro-cardiographic, Aluminium phosphide, ethylene dibromide & poisoning.

scholarly journals Scopolamine fatal outcome in an inmate after buscopan® smoking

2021 ◽  
Author(s):  
Sabina Strano-Rossi ◽  
Serena Mestria ◽  
Giorgio Bolino ◽  
Matteo Polacco ◽  
Simone Grassi ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Drug Abuse ◽  
Muscarinic Receptors ◽  
Histopathological Examination ◽  
Fatal Outcome ◽  
Post Mortem ◽  
Butyl Bromide ◽  
Cannabis Abuse ◽  
Toxic Concentration ◽  
History Of

AbstractScopolamine is an alkaloid which acts as competitive antagonists to acetylcholine at central and peripheral muscarinic receptors. We report the case of a 41-year-old male convict with a 27-year history of cannabis abuse who suddenly died in the bed of his cell after having smoked buscopan® tablets. Since both abuse of substances and recent physical assaults had been reported, we opted for a comprehensive approach (post-mortem computed tomography CT (PMCT), full forensic autopsy, and toxicology testing) to determine which was the cause of the death. Virtopsy found significant cerebral edema and lungs edema that were confirmed at the autopsy and at the histopathological examination. Scopolamine was detected in peripheral blood at the toxic concentration of 14 ng/mL in blood and at 263 ng/mL in urine, and scopolamine butyl bromide at 17 ng/mL in blood and 90 ng/mL in urine. Quetiapine, mirtazapine, lorazepam, diazepam, and metabolites and valproate were also detected (at therapeutic concentrations). Inmates, especially when they have a history of drug abuse, are at risk to use any substance they can find for recreational purposes. In prisons, active surveillance on the management and assumption of prescribed drugs could avoid fatal acute intoxication.

scholarly journals Post-mortem Examination of Genital Organs from Sows with Reproductive Disturbances in a Sow-pool

1997 ◽  
Vol 38 (3) ◽  
pp. 253-262
Author(s):  
A.-M. Dalin ◽  
K. Gidlund ◽  
L. Eliasson-Selling
Keyword(s):  
Post Mortem ◽  
Post Mortem Examination
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