Equine Sarcoid-histomorphological, Histochemical and Immunohistochemical Studies in A Thoroughbred Horse

Background: Equine sarcoid is a rare equine skin tumour seen in any age group of horses, usually in younger horses. Grossly, it appears as multinodular masses which may or may not ulcerate and pink to greyish white coloured. It was suggested that the papilloma virus is a causative organism along with predisposing factors like skin abrasions and wounds. The definitive diagnosis is based on histopathology and classified according to their gross appearance and clinical behavior. To find out the origin and proliferative nature, histochemical and immunohistochemical study was performed. The present communication reflects various patterns of sarcoid on histopathological examination and it was confirmed by histochemistry and immunohistochemistry. Methods: A four-year-old bay colt was presented to the Madras Veterinary College Teaching Hospital with nodular masses on the buccal cavity. Fine needle aspiration biopsy (FNAB), blood, serum and tumour biopsy materials were collected under local anesthesia. The collected samples were subjected to Leishman-Giemsa staining, haematological and biochemical analysis, histopathology, histochemical and immunohistochemical examination. Result: Histopathologically, there was mucosal hyperplasia and hyperkeratosis. The neoplastic cells were arranged as storiform or whorl-like pattern. Also, there was perpendicular orientation of fibroblasts towards the basement membrane (picket fence) at the dermo-epidermal junction, which was considered as characteristic feature of sarcoid. Histochemical examination with Picrosirius red revealed strong positivity characterised by deep red coloured mature collagen fibres. Immunohistochemically, the hyperplastic epithelial cells were positive for pan cytokeratin and fibroblast cells were strongly positive for vimentin. Based on the histopathology, histochemistry and immunohistochemistry, the condition was diagnosed as sarcoid which is rare in horses.

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F.N.A.C. AS DIAGNOSTIC TOOL OF PAROTID TUMORS AND ASSOCIATED PITFALLS IN CORRELATION WITH HISTOPATHOLOGY

International Journal of Medical and Biomedical Studies ◽

10.32553/ijmbs.v4i1.1153 ◽

2020 ◽

Vol 4 (1) ◽

Author(s):

Bhawana Pant ◽

Sanjay Gaur ◽

Prabhat Pant

Keyword(s):

Preoperative Diagnosis ◽

Histopathological Examination ◽

Clinical Information ◽

Final Diagnosis ◽

Needle Aspiration ◽

Histopathological Diagnosis ◽

Cytological Diagnosis ◽

Tertiary Referral Centre ◽

Clear Cut ◽

Parotid Tumors

F.NA.C has been used for ages as a safe and economical tool for fast preoperative diagnosis of parotid tumors. It has certain pitfall which sometimes leads to misdiagnosis and consequently it may have affect on treatment of the tumors. Keeping in view of the diverse classification of parotid tumors’ information from cytology should be combined with radiology as well as clinical diagnosis. Aim: To discuss some cases where there was discrepancy between cytological diagnosis and histopathological result and also suggest measures to improve the efficacy of F.N.A.C. Material and methods: The study includes 50 cases of parotid tumours who presented to the department of ENT at Government medical college Haldwani which is a tertiary referral centre during 2009 to 2016. Only adult patients were included and inflammatory swelling were excluded from the study. All patients evaluated Contrast enhanced computerized tomography(CECT) and Magnetic resonance imaging (MRI) followed by Fine needle aspiration cytology .Preoperative diagnosis was made upon the findings of the above investigations and different types of parotid surgeries were done. . Final diagnosis was made on histopathological examination. Result :The most common tumour came out to be pleomorphic adenoma (23 cases-46%) followed by mucoepidermoid carcinoma(12cases-24%). In ten cases there was no clear cut association between cytological diagnosis and final histopathological diagnosis. Conclusion: FNAC is highly sensitive and specific technique for diagnosis of many salivary gland swellings. FNAC can be used preoperatively to avoid unnecessary surgery and biopsy. Details of clinical information and radiologic features may help the pathologist to arrive at the appropriate diagnosis and reduce false interpretation. Pitfalls may also occur with improper technique of FNAC which can be overcome by proper caution.

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Pleomorphic Adenoma of the Submandibular Gland in a 10-Year-Old Child: A Case Report

Ear Nose & Throat Journal ◽

10.1177/01455613211022113 ◽

2021 ◽

pp. 014556132110221

Author(s):

Bouatay Rachida ◽

Ons Kharrat ◽

Wiem Boughzala ◽

Seifeddine Ben Hammouda ◽

Nouha Ben Abdeljelil ◽

...

Keyword(s):

Submandibular Gland ◽

Pleomorphic Adenoma ◽

Fine Needle Aspiration ◽

Histopathological Examination ◽

Preoperative Evaluation ◽

Needle Aspiration ◽

Magnetic Resonance Images ◽

Fine Needle ◽

Sebaceous Cyst ◽

Growing Mass

We report the case of a 10-year-old girl with a painless slowly growing mass that had developed over the course of 2 years in the left submandibular area. Physical examination revealed a firm painless submandibular mass with no other associated signs. Ultrasound graphics were in favor of a sebaceous cyst. Fine-needle aspiration and magnetic resonance images (MRI) concluded to a pleomorphic adenoma of the submandibular gland. The patient underwent left submandibulectomy. Histopathological examination confirmed the diagnosis of pleomorphic adenoma arising from the submandibular gland. The postoperative course was uneventful. Pleomorphic adenoma of the submandibular gland represents a diagnostic and therapeutic dilemma in children. Preoperative evaluation, including MRI and fine-needle aspiration, is recommended. Total submandibulectomy is also recommended to minimize recurrences and to avoid malignant transformation.

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Decreased Size of Mammary Tumors Caused by Preoperative Treatment with Aglepristone in Female Domestic Dogs (Canis familiaris) Do Not Influence the Density of the Benign Neoplastic Tissue Measured Using Shear Wave Elastography Technique

Animals ◽

10.3390/ani11020527 ◽

2021 ◽

Vol 11 (2) ◽

pp. 527 ◽

Cited By ~ 1

Author(s):

Barbara Pieczewska ◽

Kamila Glińska-Suchocka ◽

Wojciech Niżański ◽

Michał Dzięcioł

Keyword(s):

Shear Wave ◽

Tumor Size ◽

Histopathological Examination ◽

Shear Wave Elastography ◽

Mammary Tumors ◽

Needle Aspiration ◽

Surgical Removal ◽

Preoperative Treatment ◽

Neoplastic Tissue ◽

Before And After

Shear wave elastography (SWE) can be useful to discriminate between malignant and benign mammary tumors. In dogs with elevated progesterone levels compared to the baseline and fast-growing tumors, treatment with the use of aglepristone allows for tumor size reduction, which facilitates surgery. This study aimed to evaluate the influence of the preoperative treatment of benign mammary tumors (BMTs), performed with the use of aglepristone, on the density of the tumor tissue measured by SWE. Twelve female dogs with diagnosed BMTs and increased levels of progesterone were treated with aglepristone (Alizine, Virbac, France) at 10 mg/kg s.c. (Subcutaneous injection). twice, with a 24 h interval. The density of the tumor was evaluated by SWE before and after the treatment. The type of tumor was evaluated by fine needle aspiration cytology before treatment, and a histopathological examination was made after surgical removal, performed after the aglepristone treatment. In all the cases, a significant reduction in the mammary tumor’s size was observed following treatment, with no influence on the density of the tumor’s tissue measured by SWE. Similar studies on malignant mammary tumors are warranted to verify if in these cases, density will also be a constant parameter that is not dependent on the tumor size.

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Histopathological Analysis of Endometrial Biopsy in Gandaki Medical College Teaching Hospital, Pokhara, Nepal

Journal of Gandaki Medical College-Nepal ◽

10.3126/jgmcn.v12i1.22608 ◽

2019 ◽

Vol 12 (1) ◽

pp. 25-30

Author(s):

B Parajuli ◽

G Pun ◽

S Ranabhat ◽

S Poudel

Keyword(s):

Histopathological Examination ◽

Hydatidiform Mole ◽

College Teaching ◽

Histopathological Diagnosis ◽

Age Group ◽

Medical College ◽

Dilatation And Curettage ◽

Partial Mole ◽

Proliferative Endometrium ◽

The Common

Objective: To study the spectrum of histopathological diagnosis of endometrial lesions and their distribution according to age. Methods: All the endometrium samples obtained by the procedure of dilatation and curettage and hysterectomy sent for histopathological examination at Pathology Department of Gandaki Medical College Teaching Hospital, Pokhara, Nepal. The study duration was total 12 months ranging from July 2016 to June 2017. All the endometrial samples were processed, sectioned at 4 - 6 μm and stained with routine H & E stain. Patient’s data including age, sex, procedure of the biopsy taken and histopathological diagnosis were noted. A pathologist, using Olympus microscope, reported the slides. Cases were reviewed by a second pathologist whenever necessary. Results: A total of 128 cases were studied. The most common histopathological diagnosis was proliferative endometrium (28.9%) followed by disorder proliferative endometrium (15.65%). Most of the patients were in age group 36 - 45 years comprising 32.03%. Hydatidiform mole comprised of 7.03% and among Hydatidiform mole, partial mole was more common. Dilatation and curettage (82.8%) was the common procedure in compare to hysterectomy for the evaluation of endometrial lesions. Conclusions: In this study, we observed a variety of endometrial lesions. Most of them are benign; among benign, proliferative endometrium was the common histopathological diagnosis followed by disorder proliferative endometrium. Most common presenting age group was found to be at 36 - 45 years. In evaluation of hydatidiform mole, partial mole was more frequent in compare to complete mole. Conventional dilatation and curettage is the preferred method in developing countries with limited resource to screen endometrial lesion and therefore biopsy should be sent for histopathological examination. Thus histopathological examination of routinely stained hematoxylin and eosin is readily available and widely accepted standard technique for evaluation of the endometrial lesions.

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Cysticercosis: Hooked by a Hooklet on Fine Needle Aspiration Cytology—A Case Report

Case Reports in Infectious Diseases ◽

10.1155/2013/315834 ◽

2013 ◽

Vol 2013 ◽

pp. 1-2 ◽

Cited By ~ 1

Author(s):

Manav Sawhney ◽

Shubhra Agarwal

Keyword(s):

Fine Needle Aspiration ◽

Fine Needle Aspiration Cytology ◽

Histopathological Examination ◽

Bladder Wall ◽

Clinical Manifestations ◽

Needle Aspiration ◽

Aspiration Cytology ◽

Muscle Involvement ◽

Fine Needle ◽

Needle Aspiration Cytology

Cysticercosis is a systemic parasitic disease caused by the larval form of cestodeT. solium. It has a worldwide distribution and is potentially harmful with variable clinical manifestations. The patient most commonly presents with subcutaneous and muscle involvement in the form of nodular lesions. The other most commonly involved sites include eye, brain, bladder wall, and heart. Cysticercosis can be diagnosed on serology, and radiologically but confirmatory diagnosis is based on histopathological examination of the involved tissue biopsy specimen. Fine needle aspiration cytology is a useful low-cost outpatient procedure tool for preoperative diagnosis of cysticercosis and is absolutely essential for diagnosis of the parasitic lesions in a peripheral hospital, one like ours.

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Cytohistological Concordance of Papillary Carcinoma of Thyroid and Its Variants

Journal of Evidence Based Medicine and Healthcare ◽

10.18410/jebmh/2021/41 ◽

2021 ◽

Vol 8 (04) ◽

pp. 213-218

Author(s):

Nameera Saleem ◽

Naval Kishore Bajaj ◽

Ezhil Arasi Nagamuthu

Keyword(s):

Papillary Thyroid Carcinoma ◽

Thyroid Carcinoma ◽

Papillary Carcinoma ◽

Fine Needle Aspiration ◽

Histopathological Examination ◽

Correct Diagnosis ◽

Needle Aspiration ◽

Papillary Thyroid ◽

Fine Needle ◽

Nuclear Features

BACKGROUND Papillary thyroid carcinoma is the most common malignancy of the thyroid gland. Fine Needle Aspiration Cytology (FNAC) is a rapid, safe and economic procedure, and has a sensitivity approaching of 93.5 % and specificity close to 90 % for diagnosing papillary thyroid carcinomas. This study aims at correlating the cytological and histological diagnosis to arrive at the rate of concordance and discordance, identify variants of papillary thyroid carcinoma (PTC) on cytology and discuss the cytological mimics of PTC. METHODS Data from cases was collected over a period of three years (2015 - 2018). A descriptive study was done. Cases from Osmania General Hospital representing histologically proven cases of papillary carcinoma thyroid along with their corresponding cytological findings were analysed. Cytosmears were obtained from fine needle aspiration of thyroid lesions using a 26-gauge needle, stained with haematoxylin and eosin (H&E). Thyroidectomy specimens were fixed in 10 % buffered formalin, grossed and paraffin embedded. After processing, sections obtained by microtomy were stained with H & E for histopathologic evaluation. RESULTS The institute received a total of 258 thyroid specimens for histopathology and 686 cases for thyroid FNAC over a period of three years. This study includes 70 cases which had both cytology and histopathology correlation at our institution. 65 cases were diagnosed as PTC on histopathology and correct diagnosis was made on cytology with 73.8 % concordance (48 / 65 cases) and discordance was seen in 26.1 % (17 / 65 cases). 5 cases were misdiagnosed on cytology as PTC, and on histopathological examination were diagnosed as non-PTC. CONCLUSIONS Fine needle aspiration shows variable accuracy for PTC, ranging from 65 % to 90 %. The architectural arrangement of cells in papillary fragments and presence of nuclear features in majority of cells is diagnostic of the conventional variant of PTC. The other variants however, pose a diagnostic dilemma on account of their architectural variation, altered cytomorphology and the scant presence of nuclear features. An increase in the awareness of cytomorphology of variants and also of the mimics of PTC helps improve the diagnostic accuracy on FNAC. KEYWORDS Papillary Thyroid Carcinoma, Variants of PTC, Cytohistopathological Correlation

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A STUDY OF CORRELATION OF PREOPERATIVE FINE NEEDLE ASPIRATION CYTOLOGY (FNAC) WITH HISTOPATHOLOGICAL EXAMINATION (HPE) IN GOITRE

Journal of Evolution of Medical and Dental Sciences ◽

10.14260/jemds/2015/2198 ◽

2015 ◽

Vol 4 (89) ◽

pp. 15414-15417 ◽

Cited By ~ 2

Author(s):

Srirangaprasad K ◽

Nagaraj T M ◽

Madav D ◽

Ritesh Mahajan ◽

Praveen Kumar ◽

...

Keyword(s):

Fine Needle Aspiration ◽

Fine Needle Aspiration Cytology ◽

Histopathological Examination ◽

Needle Aspiration ◽

Aspiration Cytology ◽

Fine Needle ◽

Needle Aspiration Cytology

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Women with Abnormal Endometrial Pathology: Experienced at a Tertiary Care Hospital in Bangladesh

Journal of Shaheed Suhrawardy Medical College ◽

10.3329/jssmc.v5i1.16198 ◽

2013 ◽

Vol 5 (1) ◽

pp. 11-13

Author(s):

Mst Syeeda Showkat ◽

Lubna Khondker ◽

Shahryar Nabi ◽

Bishwajit Bhowmik

Keyword(s):

Endometrial Hyperplasia ◽

Histopathological Examination ◽

Tertiary Care ◽

Cross Sectional Study ◽

Transvaginal Sonography ◽

Common Disease ◽

Care Hospital ◽

Cross Sectional ◽

Examination Result ◽

Endometrial Pathology

Background: Transvaginal sonography is superior to transabdominal sonography in most cases of pelvic pathology. Objective: This study was undertaken to see the clinicalpathological characteristics of endometrial hyperplasia. Methodology: This cross sectional study was carried out from January 2007 to December 2008 for a period of two years. All suspected cases of endometrial hyperplasia were included for this study. Transvaginal sonography (TVS) performed and confirmation was done by histopathological examination. Result: A total number of 40 cases were enrolled for this study. The most common diagnosed endometrial pathology was endometrial hyperplasia which was 42.5% cases. Endometrial polyp was diagnosed in 32.5% cases. Endometrial carcinoma was in 7.5% patients and submucosal fibroid was in 10.0%. Conclusion: Endometrial hyperplasia is the most common disease among the women presented with endometrial pathology. DOI: http://dx.doi.org/10.3329/jssmc.v5i1.16198 J Shaheed Suhrawardy Med Coll, 2013;5(1):11-13

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SPOROTRIKOSIS LIMFOKUTAN DIAGNOSIS HISTOPATOLOGI DAN SITOLOGI

JURNAL BIOMEDIK (JBM) ◽

10.35790/jbm.2.1.2010.843 ◽

2013 ◽

Vol 2 (1) ◽

Author(s):

Poppy M. Lintong ◽

Inneke V. Sumolang

Keyword(s):

Skin Lesion ◽

Fine Needle Aspiration ◽

Fine Needle Aspiration Biopsy ◽

Histopathological Examination ◽

Sporothrix Schenckii ◽

Giant Cells ◽

Skin Lesions ◽

Needle Aspiration ◽

Fine Needle ◽

Left Hand

Abstract: Diagnosis of sporotrichosis associated with lymphocutaneous nodules was made based on the histopathological examination of skin lesions and the cytology of fine needle aspiration biopsy (FNAB). A case of sporotrichosis in a 63-year-old man was reported with papules and nodules spread along the back of the left hand, forearm, and arm. The histopatho-logical examination showed infiltration of PMNs, granulomas, and giant cells in the dermis and epidermis, along with hyperplasia and microabscesses. Sporothrix schenckii was not found in the skin lesion tissues. However, in the FNAB cytology examination of lymphocutaneus nodules we found spores of Sporothrix schenckii in the cytoplasma of histiocytes besides granuloma and infiltration of PMNs. Key words: sporothrix schenckii, histopathology, FNAB cytology. Abstrak: Diagnosis sporotrikosis kulit dengan nodul limfokutan ditegakkan melalui pemerik-saan histopatologi pada lesi kulit dan sitologi biopsi aspirasi jarum halus pada nodul limfo-kutan. Kami melaporkan kasus sporotrikosis pada laki-laki berusia 63 tahun dengan papul-papul dan nodul-nodul eritematosa pada dorsum manus, antebrakium, dan brakium sinistra. Pemeriksaan histopatologi jaringan biopsi dari lesi kulit menunjukkan reaksi radang, gambaran granuloma, dan sel datia dalam dermis dan epidermis, dengan mikroabses disertai hiperplasia. Tidak ditemukan jamur Sporothrix schenckii dalam potongan jaringan histopatologi. Hasil pemeriksaan sitologi biopsi aspirasi jarum halus pada nodul limfokutan memperlihatkan adanya spora-spora jamur Sporothrix schenckii dalam sitoplasma sel-sel histiosit disamping terdapatnya bentuk granuloma dalam infiltrat radang. Kata kunci: sporothrix schenckii, histopatologi, sitologi biopsi aspirasi jarum halus.

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Clinicopathological study of thyroid swellings

International Journal of Otorhinolaryngology and Head and Neck Surgery ◽

10.18203/issn.2454-5929.ijohns20201533 ◽

2020 ◽

Vol 6 (5) ◽

pp. 974

Author(s):

Anchal Gupta ◽

Padam Singh Jamwal

Keyword(s):

Fine Needle Aspiration ◽

Histopathological Examination ◽

Clinical Problem ◽

Neck Surgery ◽

Needle Aspiration ◽

Functional Abnormality ◽

Fine Needle ◽

Common Diagnosis ◽

Thyroid Swelling ◽

Needle Aspiration Cytology

Background: Diseases of the thyroid continue to be a common clinical problem having a prevalence rate of 4 to 7% in the general population.Methods: This retrospective study was conducted in the Department of ENT, Head and Neck Surgery, SMGS Hospital, Jammu from January 2018 to January 2020. A thorough history taking, meticulous clinical examination, routine hematological and biochemical investigations were done in all the patients. General examination of the patient was done and looked for thyroid functional abnormality followed by local examination was carried out to locate and identify the site of the swelling, shape, size and consistency of the thyroid swelling and clinical diagnosis was made. All thyroid swelling cases were subjected to fine needle aspiration cytology (FNAC). The type of thyroidectomy is based on the size and type of thyroid whether neoplastic or non-neoplastic. All the specimens were sent for histopathological examination. Results: Out of 60 patients in the study, 56 were females and only 4 were males. The most common age group was 31 to 40 years seen in 24 (40%) patients. The most common presenting symptom was swelling in the neck seen in 58 (96.67%) patients. The most common diagnosis on FNAC was colloid goiter seen in 40 (66.67%) patients. The most common was benign nodular colloid goiter seen in 35 (58.33%) patients.Conclusions: Fine needle aspiration is a very useful and indispensible test in the diagnosis of thyroid lesions.

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