Gross, Microanatomical and Ultrastructural Studies of Interdigital Gland in Madras Red Sheep (Ovis aries)

Background: Madras Red sheep is a well-recognized meat type breed reared only on free range system to yield tasty meat. The aim of this study was to determine the gross, microanatomical and ultrastructural details of the interdigital gland in Madras Red sheep. Methods: The interdigital glands were removed immediately after slaughter and subjected for gross morphological, histological, immunohistochemical studies and ultrastructural observations. Result: The results revealed that a well-developed tobacco-pipe shaped interdigital gland was present in all the four limbs which was composed of an orifice, excretory duct, body and bent or flexure. Histologically, wall of the gland was composed of epidermis, dermis and capsule from within outward. Epidermis was made of stratified squamous keratinized epithelium. Dermis was composed of dense irregular connective tissue with sweat, sebaceous glands, arrectores pili muscle and hair follicles were embedded within. The sweat gland appeared as group of tortuous tubules under scanning electron microscope. The oily secretion of the gland helps in maintaining the healthiness of foot during movement of the animal.

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Histology, lectin histochemistry and ultrastructure of interdigital gland in crossbred sheep

10.51128/jfas.2020.a020 ◽

2020 ◽

Vol 1 (2) ◽

pp. 112-116

Author(s):

S. Maya ◽

A. R. Sreeranjini ◽

C. Leena ◽

N.S. Sunil Kumar ◽

K.B. Sumena ◽

...

Keyword(s):

Squamous Epithelium ◽

Dorsal Surface ◽

Lectin Histochemistry ◽

Nerve Fibers ◽

Hair Follicles ◽

Sweat Glands ◽

Ulex Europaeus ◽

Ultrastructural Studies ◽

Interdigital Gland ◽

Glandular Secretion

Abstract: Histology, lectin histochemistry and ultrastructure of interdigital gland in sheep was studied using six adult cross bred sheep of either sex collected from the Meat Technology Unit, College of Veterinary and Animal Sciences, Mannuthy. The manus and pes were detached from the rest of limbs and after recording the gross observations, the interdigital glands were fixed in 10 per cent neutral buffered formalin and processed for histological examinations. Stratified squamous epithelium with a prominent keratin layer lined the lumen, similar to the skin over the dorsal surface of manus and pes. The epidermis presented mucosal folds, which projected into the lumen. The dermis contained small sebaceous glands, hair follicles of different sizes, arrector pili muscles and apocrine sweat glands. The secretory epithelium of sweat glands was lined with a simple layer of cuboidal and relatively flattened cells. The excretory ducts of these glands were lined by cuboidal cells, with darker appearance than its secretory portion. The fibrous capsule was composed of dense connective tissue with collagen, adipose cells, blood vessels and nerve fibers. Glandular secretion and the granules in the stratum granolosum of the surface squamous epithelium showed positive reaction for lectin Ulex europaeus (UEA-I). The apocrine nature of sweat glands was revealed in the ultrastructural studies by scanning electron microscope. Keywords: Interdigital gland, sheep, histology, lectin histochemistry and ultrastructure

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Lectin histochemical study of the quill sebaceous gland in the dorsal skin of the Sunda porcupine (Hystrix javanica)

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d200932 ◽

2019 ◽

Vol 20 (9) ◽

Author(s):

Andhika Yudha Prawira ◽

SAVITRI NOVELINA ◽

HUDA SALAHUDIN DARUSMAN ◽

SRIHADI AGUNGPRIYONO ◽

WARTIKA ROSA FARIDA

Keyword(s):

Sebaceous Gland ◽

Histochemical Study ◽

Lectin Binding ◽

Lectin Histochemistry ◽

Hair Follicles ◽

Excretory Duct ◽

Sugar Residue ◽

Dorsal Skin ◽

Sebaceous Glands ◽

Complex Type

Abstract. Prawira AY, Novelina S, Farida WR, Darusman HS, Agungpriyono S. 2019. Lectin histochemical study of the quill sebaceous gland in the dorsal skin of the Sunda porcupine (Hystrix javanica). Biodiversitas 20: 2677-2684. In the Sunda Porcupine skin, the sebaceous glands of the quill follicles are multi-lobed alveolar and better developed than those of hair follicles. Using lectin histochemistry, we have studied the distribution of sugar-binding in the sebaceous glands of quills in comparison with those of hairs in six adult Sunda Porcupines. The skin samples from the thoracodorsal and lumbosacral regions were collected by biopsy procedure and processed for histology, histochemistry, and lectin histochemistry. The results showed that the lectin binding patterns are similar in secretory acinar parts of both types of sebaceous glands. The acini and excretory duct contained neutral carbohydrate and sugar residues. The acini also contained alpha D-mannose sugar residue, while the non-secretory excretory duct and debris in the lumen contained alpha-D-mannose, alpha-L-fucose, and α>β-N-acetylgalactosamine, and complex type N-glycan (oligosaccharide) sugar residues, as well. The present findings allowed us to suggest, that in Sunda Porcupine functions of the sebaceous glands of quills are more complex and active compared to those of the hairs.

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Scanning electron microscopic features of the ovine interdigital sinus

Acta Veterinaria Hungarica ◽

10.1556/avet.55.2007.4.1 ◽

2007 ◽

Vol 55 (4) ◽

pp. 417-424 ◽

Cited By ~ 3

Author(s):

S. Karahan ◽

D. Yildiz ◽

D. Bolat

Keyword(s):

Skin Surface ◽

Hair Follicles ◽

Fibrous Capsule ◽

Sebaceous Glands ◽

Electron Microscopic ◽

Apocrine Glands ◽

Apocrine Secretion ◽

Scanning Electron Microscopic ◽

Microscopic Features ◽

Scanning Electron

This article describes the scanning electron microscopic (SEM) features of the ovine interdigital sinus. The lumen was filled with a dense secretory material and quite a number of hairs embedded in the luminal content. For SEM purposes, the sinus was divided into three parts: base, body and neck. At the cut surface, the wall exhibited significant folds which were almost absent in the base, the very short blind end of the sinus. The wall had three layers: epidermis, dermis and fibrous capsule. Stratified epithelium with a prominent keratin layer faced the lumen. The inner surface was similar to the skin surface; however, it was coarser due to folds. The fibrous capsule was composed mainly of dense connective tissue, constituting the outermost layer of the wall. The dermis contained common skin structures including sebaceous glands, hair follicles, arrector pili muscles and apocrine glands. Sebaceous glands appeared as groups of bubbles if they were not collapsed. Apocrine glands generally appeared as a group of coiled tubules. They frequently exhibited apocrine blebs, which is a feature of apocrine secretion. SEM was able to locate some secretory vesicles in the lumen of apocrine tubules which is frequently filled by secretory content. Thus, the apocrine tubules exhibited classical features of apocrine secretion.

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Visualization of Internal Skin Structures with the Scanning Electron Microscope

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100062130 ◽

1969 ◽

Vol 27 ◽

pp. 46-47

Author(s):

Emil Bernstein

Keyword(s):

Electron Microscope ◽

Scanning Electron Microscope ◽

Great Depth ◽

Hair Follicles ◽

Depth Of Field ◽

Pig Skin ◽

Realistic Picture ◽

Main Components ◽

Scanning Electron

An interesting method for examining structures in g. pig skin has been developed. By modifying an existing technique for splitting skin into its two main components—epidermis and dermis—we can in effect create new surfaces which can be examined with the scanning electron microscope (SEM). Although this method is not offered as a complete substitute for sectioning, it provides the investigator with a means for examining certain structures such as hair follicles and glands intact. The great depth of field of the SEM complements the technique so that a very “realistic” picture of the organ is obtained.

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A histological examination of the holding sacs and glandular scent organs of some bat species (Emballonuridae, Hipposideridae, Phyllostomidae, Vespertilionidae, and Molossidae)

Canadian Journal of Zoology ◽

10.1139/z99-248 ◽

2000 ◽

Vol 78 (4) ◽

pp. 613-623 ◽

Cited By ~ 29

Author(s):

William MR Scully ◽

M B Fenton ◽

A SM Saleuddin

Keyword(s):

Electron Microscope ◽

Sexual Dimorphism ◽

Sebaceous Glands ◽

Microscope Examination ◽

Scent Gland ◽

Light Microscope Level ◽

Histological Techniques ◽

Saccopteryx Bilineata ◽

The Face ◽

Scanning Electron

Using histological techniques at the light-microscope level, we examined and compared structure and sexual dimorphism of the wing sacs and integumentary glandular scent organs of 11 species of microchiropteran bats. The antebrachial wing sacs of the Neotropical emballonurids Peropteryx macrotis, Saccopteryx bilineata, and Saccopteryx leptura differed in size and location but lacked sudoriferous and sebaceous glands, confirming that they were holding sacs rather than glandular scent organs. Glandular scent organs from 11 species consisted of sebaceous and (or) sudoriferous glands in emballonurids (P. macrotis, S. bilineata, S. leptura, Taphozous melanopogon, Taphozous nudiventris), hipposiderids (Hipposiderous fulvus, Hipposiderous ater), the phyllostomid Sturnira lilium, the vespertilionid Rhogeessa anaeus, and molossids (Molossus ater and Molossus sinaloe). Glandular scent organs were located on the face (H. fulvus, H. ater), gular region (S. bilineata, P. macrotis, T. melanopogon, M. ater, M. sinaloe), chest (T. nudiventris), shoulder (S. lilium), or ears (R. anaeus). Glandular scent organs showed greater similarities within than between families, and typically were rudimentary or lacking in females. Scanning electron microscope examination revealed that the hairs associated with glandular areas of male T. melanopogon were larger and had a different cuticular-scale pattern than body hairs. These were osmetrichia, hairs specialized for holding and dispersing glandular products. In S. lilium, hairs associated with the shoulder scent-gland area were larger than body hairs but similar in cuticular-scale pattern.

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Sonophoretic acceleration of degradation process for vaterite particles delivered into the hair follicles

Izvestiya of Saratov University New series Series Physics ◽

10.18500/1817-3020-2021-21-1-80-85 ◽

2021 ◽

Vol 21 (1) ◽

pp. 80-85

Author(s):

Yulia I. Svenskaya ◽

◽

Elina A. Genina ◽

Valery V. Tuchin ◽

◽

...

Keyword(s):

Degradation Process ◽

Hair Follicles ◽

Bioactive Molecules ◽

Microscopy Investigation ◽

Electron Microscopy Investigation ◽

Minute Post ◽

Scanning Electron Microscopy Investigation ◽

Particulate Delivery ◽

Scanning Electron

Intrafollicular drug delivery is beneficial in terms of both localized therapy of relevant skin disorders and systemic transportation of bioactive molecules. Vaterite particles are capable of loading and delivering various substances to hair follicles. Possibility to control the duration of their intrafollicular degradation can improve such a particulate delivery system. Here, we propose the use of sonophoresis (1 MHz, 1 W/cm2 ) to accelerate the resorption of vaterite carriers inside the hair follicles of rats in vivo. The effect of sonication is demonstrated utilizing optical coherence tomography monitoring of the skin and scanning electron microscopy investigation of the plucked hairs. A nine-minute post-treatment of skin in the site of particle delivery allowed us to almost halve the time of their degradation.

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Communication compartments in hair follicles and their implication in differentiative control

Development ◽

10.1242/dev.114.2.389 ◽

1992 ◽

Vol 114 (2) ◽

pp. 389-393 ◽

Cited By ~ 1

Author(s):

E. Kam ◽

M.B. Hodgins

Keyword(s):

Lucifer Yellow ◽

Early Stage ◽

Dermal Papilla ◽

Hair Follicles ◽

Hair Dye ◽

Hair Bulb ◽

Ultrastructural Studies ◽

Junctional Communication ◽

Root Sheath ◽

Inner Root Sheath

Observations on hair follicles presented in this paper show that boundaries to junctional communication are formed between groups of cells following different pathways of differentiation. The patterns of junctional communication in the bulbs of rat vibrissa follicles and human hair follicles were studied by microinjection of the fluorescent tracer dye Lucifer Yellow CH. Dye spread was extensive between undifferentiated cells of the hair bulb matrix but communication boundaries were found between groups of morphologically distinct cells. For example, boundaries to dye spread were observed between undifferentiated matrix cells and cells in the early stage of differentiation into the inner root sheath, between Huxley's and Henle's layers in the early inner root sheath and between cells of the cuticle and cortex of the hair. Dye did not spread between epithelial cells of the hair bulb and mesenchymal cells of the connective tissue sheath or dermal papilla. The patterns of dye spread became more complex (increased boundary formation and subcompartmentation) as differentiation progressed in higher regions of the hair bulb. The observed communication can be related to previous ultrastructural studies by others on the distribution of gap junctions in the wool follicle. These results show that junctional communication, with its consequent intercellular spread of small ions and molecules, is associated with uniformity of expression and behaviour within cell populations and that interruption of communication through the formation of boundaries and communication compartments is temporally and spatially related to the production of subpopulations of cells committed to the expression of different phenotypes.

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