Study of Prolactin Receptor Gene (PRLR5) polymorphisms and its association with egg production in Kadaknath hens

2017 ◽  
Author(s):  
Shiv Kumar Yadav ◽  
S.K. Maurya ◽  
Alok Kumar Yadav ◽  
Anand Kumar ◽  
Kamalesh Yadav
Keyword(s):  
Egg Production ◽  
Receptor Gene ◽  
Prolactin Receptor ◽  
Production Traits ◽  
Agarose Gel Electrophoresis ◽  
Specific Primer ◽  
Wing Vein ◽  
Polygenic Inheritance ◽  
Final Volume ◽  
Pcr Products

The present study was conducted to investigate the polymorphisms of prolactin receptor (PRLR5) gene and its association with egg production in Kadaknath hens. Egg production is a polygenic inheritance trait. Study was conducted on twenty female birds of Kadaknath kept for laying. Egg production performances were recorded as age at first laying (AFE), Body Weight at First Egg (WFE), Mean Egg Weight (MEW) and Total No. of Eggs at 90 days of laying (TEN). Genomic DNA isolated from 2- 3 of blood collected from wing vein of each bird was amplified for prolactin receptor (PRLR5) gene with specific primer by standardizing and optimising the PCR protocols. PCR was performed in a final volume of 20 ml. The amplified PCR products were resolved on the gels to generate polymorphisms. PRLR5 was digested with BamHI. Retriction digested products were run on 2% agarose gel electrophoresis. PRLR5 showed two alleles and two genotypes. The frequency of AA genotype at this locus was 0.75 and BB genotype was 0.25. The AFE (d), WFE (Kg), MEW (g) and TEN of Kadaknath hens in the present study were found to be 188.000.71, 1.260.03, 42.830.21 and 37.750.59 respectively. Birds with AA genotype of PRLR5 had a significantly (P less than 0.05) better WFE and AFE than BB genotype. Prolactin receptor (PRLR5) genes produced polymorphism in Kadaknath and were associated with egg production traits.

scholarly journals Genetic effect of the prolactin receptor gene on egg production traits in chickens

2012 ◽  
Vol 11 (4) ◽  
pp. 4307-4315 ◽  
Author(s):  
L. Zhang ◽  
D.Y. Li ◽  
Y.P. Liu ◽  
Y. Wang ◽  
X.L. Zhao ◽  
...  
Keyword(s):  
Egg Production ◽  
Receptor Gene ◽  
Prolactin Receptor ◽  
Genetic Effect ◽  
Production Traits ◽  
Prolactin Receptor Gene

scholarly journals Polymorphism of the melanocortin receptor gene and its association with egg production traits in Lohmann Brown chickens

Genetika ◽  
2018 ◽  
Vol 50 (1) ◽  
pp. 317-323
Author(s):  
Sarah Aggag ◽  
Karim El-Sabrout
Keyword(s):  
Candidate Gene ◽  
Egg Production ◽  
Sequence Data ◽  
Receptor Gene ◽  
Melanocortin Receptor ◽  
Production Traits ◽  
Egg Weight ◽  
Blood Samples ◽  
Mc4r Gene ◽  
Pcr Products

The melanocortin gene (MC4R) was proposed as a candidate gene in this study for egg production traits (yield and weight) in Lohmann Brown hens. Two different primers from MC4R gene (MC4R-1 and MC4R-2) were investigated. DNA from blood samples was extracted to amplify the MC4R gene and the purified PCR products were sequenced. Alignment of sequence data from each group revealed that there is a variation in MC4R-1 at nucleotide 22 (T-G) (sense mutation) for high egg weight. Hens with the genotype produced significantly (P<0.05) higher egg weight compared to hens with the AA and BB genotypes. There was no significant (P>0.05) effect of this mutation on egg yield. There was no variation detected in MC4R-2. The detected mutation and the analysis of egg production means revealed a significant association between MC4R polymorphism and egg weight. The MC4R-SNP could be considered as a useful marker in chicken selection especially for egg weight.

Polymorphism of Prolactin Receptor Gene and its Association with Egg Production Traits in Erlang Mountainous Chicken

2012 ◽  
Vol 7 (11) ◽  
pp. 1183-1190 ◽  
Author(s):  
Ling-Bin Liu ◽  
Di-Yan Li ◽  
Xiao-Ling Zhao ◽  
Yi-Ping Liu ◽  
Yan Wang ◽  
...  
Keyword(s):  
Egg Production ◽  
Receptor Gene ◽  
Prolactin Receptor ◽  
Production Traits ◽  
Prolactin Receptor Gene

Mutations in the exon 10 of prolactin receptor gene change the egg production performance in Wanjiang white goose

2011 ◽  
Vol 39 (1) ◽  
pp. 475-483 ◽  
Author(s):  
Jie Chen ◽  
Huiling Liu ◽  
Yafei Cai ◽  
Genlin Wang ◽  
Honglin Liu ◽  
...  
Keyword(s):  
Egg Production ◽  
Receptor Gene ◽  
Prolactin Receptor ◽  
Production Performance ◽  
Prolactin Receptor Gene ◽  
Exon 10

scholarly journals PSIII-3 Association of heat shock factor protein 1 and prolactin receptor variants with molecular breeding values in Senepol cattle

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 230-230
Author(s):  
Bianca P Ortiz-Uriarte ◽  
Edgar J Soto Moreno ◽  
Lourdes E López-Nieves ◽  
Verónica M Negrón-Pérez ◽  
Americo Casas ◽  
...  
Keyword(s):  
Heat Shock ◽  
Molecular Breeding ◽  
Receptor Gene ◽  
Prolactin Receptor ◽  
Average Daily Gain ◽  
Missense Variant ◽  
Production Traits ◽  
Breeding Values ◽  
Weight Effect ◽  
Slick Hair

Abstract Thermal stress has been associated with lower food intake and growth rate in beef cattle. The purpose of this study was to assess the segregation of the p.Leu462* nonsense mutation in the prolactin receptor gene (PRLR) responsible for slick hair (SH) phenotype in Senepol and a T &gt;C missense variant (V344A) located in the heat shock transcription factor protein 1 gene (HSF1). We compared the potential association of these polymorphisms with molecular breeding values (MBV) that were estimated using a panel of commercial genetic markers (Igenity, Neogen Corp). For this, 29 females and 21 males were genotyped by PCR and sequencing. Three genotypes were observed for the p.Leu462* with frequencies of 0.02 C/C, 0.16 C/- and 0.82 -/-. For V344A only two genotypes were observed with frequencies of 0.86/TT and 0.14/TC. This polymorphism was in linkage disequilibrium with a C &gt;T synonymous variant; therefore, for the statistical analyses, the HSF1 genotypes were combined as TT/CC and TC/CT. The GLIMMIX procedure and Tukey test of SAS were used (effect of sex, HSF1 and SH genotype and interactions). MBV for production traits such as average daily gain, tenderness, yearling weight (YW), marbling, fat thickness (FT), percent choice (PC), ribeye area (RA) and carcass weight. Effect of sex (P &lt; .05) were found for YW (males: 6.93lbs ± 0.53, female: 5.74lbs ± 0.57) and for PC (female: 16.17±0.75, male: 15.02±0.55). Differences between HSF1 genotype combinations were significant for the MBV of RA (TC/CT: 0.96in2±0.10; TT/CC: 0.781in2±0.07 and for FT (TC/CT: 0.16in±0.02; TTCC: 0.12in±0.01). The similarity in segregation of SH responsible genotypes (C/- and -/-) and HSF1 (TC/CT and TT/CC) in Senepol could indicate a potential role of HSF1 in adaptation to heat stress in cattle with an additional effect in muscling and fat deposition.

scholarly journals Effects of Essential Oils-Based Supplement and Salmonella Infection on Gene Expression, Blood Parameters, Cecal Microbiome, and Egg Production in Laying Hens

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 360
Author(s):  
Georgi Yu. Laptev ◽  
Elena A. Yildirim ◽  
Larisa A. Ilina ◽  
Valentina A. Filippova ◽  
Ivan I. Kochish ◽  
...  
Keyword(s):  
Egg Production ◽  
Salmonella Enteritidis ◽  
Laying Hens ◽  
Expression Profiles ◽  
Blood Parameters ◽  
Post Inoculation ◽  
Production Traits ◽  
Immunological Parameters ◽  
Specific Expression ◽  
Blood Biochemical

One of the main roles in poultry resistance to infections caused by Salmonella is attributed to host immunity and intestinal microbiota. We conducted an experiment that involved challenging Lohmann White laying hens with Salmonella Enteritidis (SE), feeding them a diet supplemented with an EOs-based phytobiotic Intebio®. At 1 and 7 days post-inoculation, the expression profiles of eight genes related to immunity, transport of nutrients in the intestine, and metabolism were examined. Cecal microbiome composition and blood biochemical/immunological indices were also explored and egg production traits recorded. As a result, the SE challenge of laying hens and Intebio® administration had either a suppressive or activating effect on the expression level of the studied genes (e.g., IL6 and BPIFB3), the latter echoing mammalian/human tissue-specific expression. There were also effects of the pathogen challenge and phytobiotic intake on the cecal microbiome profiles and blood biochemical/immunological parameters, including those reflecting the activity of the birds’ immune systems (e.g., serum bactericidal activity, β-lysine content, and immunoglobulin levels). Significant differences between control and experimental subgroups in egg performance traits (i.e., egg weight/number/mass) were also found. The phytobiotic administration suggested a positive effect on the welfare and productivity of poultry.

Association of the Leu127 variant of the bovine growth hormone (bGH) gene with increased yield of milk, fat, and protein in Australian Holstein-Friesians

2000 ◽  
Vol 51 (4) ◽  
pp. 515 ◽  
Author(s):  
M. R. Shariflou ◽  
C. Moran ◽  
F. W. Nicholas
Keyword(s):  
Growth Hormone ◽  
Milk Production ◽  
Milk Fat ◽  
Bovine Growth Hormone ◽  
Data Sets ◽  
Production Traits ◽  
Milk Production Traits ◽  
Maximum Benefit ◽  
Pcr Products ◽  
Trait Locus

The occurrence of the Leu127/Val127 variants of the bovine growth hormone (bGH) gene and their effect on milk production traits was investigated in Australian Holstein-Friesian cattle. Animals were genotyped for the Leu127/Val127 variants, with RFLP methodology, using PCR and AluI digestion of PCR products (AluI-RFLP). Alleles Leu127 and Val127 occurred with frequencies of 82% and 18%, respectively. The quantitative effect of this polymorphic site on milk-production traits was estimated from lactation data and test-day data. Results from the 2 data sets consistently showed that the Leu127 allele is associated with higher production of milk, fat, and protein and is dominant to Val127. The average effects of the gene substitution are 95 L for milk yield, 7 kg for fat yield, and 3 kg for protein yield per lactation. This locus may be directly responsible for quantitative variation or it may be a marker for a closely linked quantitative trait locus (QTL) for milk-production traits in Australian dairy cattle. In either case, it will be useful as an aid to selection for improvement of milk production traits. As the Leu127 allele is dominant, selection of AI sires homozygous for the Leu127 allele (Leu127/Leu127) will result in maximum benefit without the need for genotyping cows.

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