In-Vivo and field evaluation of spinetoram 12 SC against Lampides boeticus on pigeonpea

2017 ◽  
Author(s):  
A. Sanjeevi Kumar ◽  
N. Muthukrishnan
Keyword(s):  
Acute Toxicity ◽  
Grain Yield ◽  
Field Evaluation ◽  
Field Conditions ◽  
Emamectin Benzoate ◽  
Toxicity Studies ◽  
Biological Insecticide ◽  
Cent Mortality ◽  
Third Instar Larvae

The young larvae of L. boeticus damage flowers and pods. A new biological insecticide molecule, spinetoram 12 SC was evaluated for acute toxicity on greenhouse environment reared L. boeticus and persistence on pigeonpea pods at laboratory; and effect on L. boeticus on pigeonpea at field conditions during 2012-2013 and 2013-2014. Acute toxicity studies revealed that LC50‘s of spinetoram on third instar larvae after 24, 48 and 72 hours after treatment were 6.71, 2.23 and 1.28 ppm respectively. In persistence studies, spinetoram 12 SC 27 g a.i./ha was upto 11 DAT and 14 DAT for spinetoram 12 SC 36 and 45 g a.i./ha. More than 50 per cent mortality was observed in spinetoram 12 SC at 45 g a.i./ha upto 9 DAT, spinetoram 12 SC at 27 and 36 g a.i./ha, spinosad 45 SC 78 g a.i./ha and emamectin benzoate 5 SG at 11 g a.i./ha upto 7 DAT, monocrotophos 36 SL at 500 g a.i./ha upto 5 DAT, respectively. Results indicated that spinetoram 12 SC was significantly effective at 36 and 45 g a.i./ha when sprayed thrice at 15 days interval and minimized the incidence of L. boeticus on pigeonpea plants and increases the grain yield. All the spinetoram doses did not show any phytotoxic symptoms on pigeonpea plants.

Field evaluation of spinetoram 12 SC against Maruca vitrata on pigeonpea

2017 ◽  
Vol 40 (04) ◽  
Author(s):  
A. Sanjeevi Kumar ◽  
A. Pavviya
Keyword(s):  
Acute Toxicity ◽  
Tamil Nadu ◽  
Field Experiments ◽  
Field Evaluation ◽  
Maruca Vitrata ◽  
Field Conditions ◽  
Flower Buds ◽  
Emamectin Benzoate ◽  
Agricultural College ◽  
Biological Insecticide

The acute and persistent toxicity experiments was conducted in the Insectary of Agricultural College and Research Institute (ACandRI), Maduari, Tamil Nadu Agricultural University. Two field experiments were conducted at farmer’s field at Jadhi Goundanpatti, Attur Block, Dindigul district, Tamil Nadu. The incidence of M. vitrata (Geyer) has been increasing for the last few years and the larvae bore inside the flower buds, flowers and the green pods and cause upto 40-70 per cent damage. A new biological insecticide molecule, spinetoram 12 SC was evaluated for acute toxicity on laboratory reared M. vitrata population, and persistence on pigeonpea leaves at laboratory conditions; and effect on M. vitrata on pigeonpea at field conditions during 2012-13 and 2013-2014 seasons. Acute toxicity studies revealed that LC50‘s of spinetoram on third instar larvae after 24, 48 and 72 hours after treatment were 4.30, 1.95 and 1.46 ppm respectively. In persistence studies, Persistence was upto 11 DAT for spinetoram 12 SC 27 g a.i./ha, spinosad 45 SC 78 g, emamectin benzoate 5 SG at 11 g a.i./ha, and monocrotophos 36 SL at 500 g; and 14 DAT for spinetoram 12 SC 36 and 45 g a.i./ha. There was a reduction in the mortality of M. vitrata larvae as the time increased and there was no mortality at 21 DAT. Results indicated that spinetoram 12 SC was significantly effective at 36 and 45 g a.i./ha when sprayed thrice at 15 days interval and minimized the incidence of leaf feeder, M. vitrata on pigeonpea plants.

scholarly journals Acute and Subacute Toxicity Assessment of Ethyl Acetate Extracts from Aerial Parts of Clerodendrum thomsoniae Balf.f in Rodents

2021 ◽  
Vol 11 (6) ◽  
pp. 13952-13961
Keyword(s):  
Acute Toxicity ◽  
Ethyl Acetate ◽  
Human Subjects ◽  
Female Rats ◽  
Laboratory Animals ◽  
Oral Toxicity ◽  
Subacute Toxicity ◽  
Toxicity Studies ◽  
Male And Female Rats

Clerodendrum is a genus of about 500 species belongs to the family Lamiaceae. Several species of this genus have been proved for the treatment of various diseases. Clerodendrum thomsoniae Balf.f were selected in this study; according to the literature available, there is no systematic toxicity studies for this plant were described. The current study was therefore carried out to evaluate the acute and sub-acute toxicity in mice and rats. The acute oral toxicity study was performed in mice following OECD guidelines 425, and the sub-acute toxicity was performed in male and female rats following OECD guidelines 407. The results showed that mice given a single dose of up to 2000 mg/kg orally did not show any toxicity signs or mortality. In the sub-acute toxicity analysis in rats, 3 specific daily doses of 150, 300, and 600 mg/kg for 28 days did not induce any major changes to the hematological and biochemical parameters. Histopathological studies revealed normal architecture that did not indicate any morphological disturbances. In our study, no deaths or any signs of toxicity were found in acute and subacute toxicity studies after oral administration according to OECD guidelines, which concluded that ethyl acetate extract of Clerodendrum thomsoniae Balf (EACT) could use for in vivo biological activity studies in laboratory animals to explore its various medicinal activity before study in human subjects.

scholarly journals In vivo anti-psoriasis, anti-inflammatory activities and oral toxicity studies of aqueous extract from seeds of Ammi visnaga L.

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 1025-1037
Author(s):  
Ez-zahir Abdeljaouad ◽  
Naya Abdallah ◽  
Seddik Nadia ◽  
Marnissi Farida ◽  
Belghmi Khalid ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Aqueous Extract ◽  
Control Group ◽  
Oral Toxicity ◽  
Distribution Width ◽  
Toxicity Studies ◽  
Ammi Visnaga ◽  
Significant Difference ◽  
Anti Inflammatory

Ammi visnaga L (A.V.) is used in the traditional medicine for the treatment of kidney stones, diabetes and vitiligo. An in vivo anti-psoriasis, anti-inflammatory activities and oral toxicity studies of aqueous extract from seeds of Ammi visnaga L. was investigated. In the acute toxicity, the extract was administered orally in a single dose to rats (0- 2000-5000 mg/kg) and in the sub-acute toxicity daily for 28 days (0-300-600-1000 mg/kg/day). The symptoms of toxicity and mortality have been recorded daily and during 14 days of recovery with an examination of liver, kidney, hematologic, biochemical and histological analysis at the end of treatment. The anti-inflammatory activity was evaluated by induction of oedema and the anti-psoriasis by induction of a psoriasiform-like skin phenotype by UV-B radiations. No mortality was observed after single gavages by a dose up to 5000 mg/kg and no signs of toxicity noted. Clinical and biochemical examination during 28 days of gavages at all doses showed no significant difference compared to control group, while a significant reduction in MCV (mean corpuscular volume) and P-LCR (platelet large cell ratio) (p <0.05), PDW (platelet distribution width) and MPV (multi-purpose vehicle) (p <0.01) was observed and histopathological examinations showed slight inflammation in the liver and kidneys for the higher dose. Percentage of inhibition of the oedema was near the positive control 50% for all doses tested. Treatment with A.V. extract had decreased the thickness of the skin induced by UV-B irradiation.  In conclusion, the LD50 was estimated greater than 5000 mg/kg; therefore A.V. can be classified as non-toxic but if used in the long term can induce a slight toxicity dose dependant with high anti-psoriasis and anti-inflammatory activities.

scholarly journals Pragmatic Toxicity Profiling of a Salubrious Polyherbal Combination of Tinospora Cordifolia, Withania Somnifera, and Boerrhavia Diffusa in Swiss Albino Mice

2020 ◽  
Vol 11 (3) ◽  
pp. 4240-4252
Author(s):  
Arshiya Shamim ◽  
Hefazat H Siddiqui ◽  
Tarique Mahmood ◽  
Mohd Haris Siddiqui ◽  
Paramdeep Bagga ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Withania Somnifera ◽  
Late Toxicity ◽  
Tinospora Cordifolia ◽  
Toxicity Screening ◽  
Subacute Toxicity ◽  
Toxicity Studies ◽  
Study Results ◽  
Pharmacological Potential

Acute and subacute toxicity screening of novel entrants in the stream of polyherbal formulations opens new doors towards the scientific approach of establishing them in the clinical market. Toxicity profiling asserts the humongous pharmacological potential that the natural herbs hold and establishes their safety profile. To exemplify this statement, this study further deals with acute and subacute toxicity studies of an olive oil-based polyherbal combination (PHC). This combination has been developed with the view that it proves to be clinically effective in diabetic cardiomyopathy and neuropathy. However, until now, the acute and subacute toxicity studies have been done on the developed PHC, and experimentally effective dose combinations have been identified. The follow-up part of this research includes the screening of the PHC against specific in vivo screening models in its initial stages. This research paper strictly summarises the acute and subacute profile of the salubrious polyherbal combination. Three herbs with strong literature background were selected: Tinospora cordifolia, Withania somnifera and Boerrhavia diffusa; and their crude extracts were prepared. The reported ED50 values of each of these plants were selected and mixed in 5 different combinations and subjected to Acute Toxicity studies. It was followed by the 28-days subacute toxicity screening of 9 different combinations that were elucidated from the acute toxicity study results. The findings were supported with recovery group studies to conform to any late toxicity symptoms of the polyherbal combination. The findings of this study helped in reaching some pathbreaking conclusions on the polyherbal combination designed.

scholarly journals Aceclofenac nanocrystals with enhanced in vitro, in vivo performance: formulation optimization, characterization, analgesic and acute toxicity studies

2017 ◽  
Vol Volume 11 ◽  
pp. 2443-2452 ◽  
Author(s):  
Haroon Rahim ◽  
Abdul Sadiq ◽  
Shahzeb Khan ◽  
Mir Azam Khan ◽  
Syed Muhammad Hassan Shah ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Formulation Optimization ◽  
Toxicity Studies

Evaluation of the In Vivo Acute Toxicity and In Vitro Hemolytic and Immunomodulatory Activities of the Moringa oleifera Flower Trypsin Inhibitor (MoFTI)

2020 ◽  
Vol 27 ◽  
Author(s):  
Leydianne Leite de Siqueira Patriota ◽  
Dayane Kelly Dias do Nascimento Santos ◽  
Bárbara Rafaela da Silva Barros ◽  
Lethícia Maria de Souza Aguiar ◽  
Yasmym Araújo Silva ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Trypsin Inhibitor ◽  
Hemolytic Activity ◽  
Moringa Oleifera ◽  
Biological Activities ◽  
Hematological Parameters ◽  
Behavioral Alterations ◽  
Female Mice

Background: Protease inhibitors have been isolated from plants and present several biological activities, including immunomod-ulatory action. Objective: This work aimed to evaluate a Moringa oleifera flower trypsin inhibitor (MoFTI) for acute toxicity in mice, hemolytic activity on mice erythrocytes and immunomodulatory effects on mice splenocytes. Methods: The acute toxicity was evaluated using Swiss female mice that received a single dose of the vehicle control or MoFTI (300 mg/kg, i.p.). Behavioral alterations were observed 15–240 min after administration, and survival, weight gain, and water and food consumption were analyzed daily. Organ weights and hematological parameters were analyzed after 14 days. Hemolytic activity of MoFTI was tested using Swiss female mice erythrocytes. Splenocytes obtained from BALB/c mice were cultured in the absence or presence of MoFTI for the evaluation of cell viability and proliferation. Mitochondrial membrane potential (ΔΨm) and reactive oxygen species (ROS) levels were also determined. Furthermore, the culture supernatants were analyzed for the presence of cytokines and nitric oxide (NO). Results: MoFTI did not cause death or any adverse effects on the mice except for abdominal contortions at 15–30 min after administration. MoFTI did not exhibit a significant hemolytic effect. In addition, MoFTI did not induce apoptosis or necrosis in splenocytes and had no effect on cell proliferation. Increases in cytosolic and mitochondrial ROS release, as well as ΔΨm reduction, were observed in MoFTI-treated cells. MoFTI was observed to induce TNF-α, IFN-γ, IL-6, IL-10, and NO release. Conclusion: These results contribute to the ongoing evaluation of the antitumor potential of MoFTI and its effects on other immunological targets.

Chemosensitizing Activity of Histone Deacetylases Inhibitory Cyclic Hydroxamic Acids for Combination Chemotherapy of Lymphatic Leukemia

2018 ◽  
Vol 18 (4) ◽  
pp. 365-371 ◽  
Author(s):  
Denis V. Mishchenko ◽  
Margarita E. Neganova ◽  
Elena N. Klimanova ◽  
Tatyana E. Sashenkova ◽  
Sergey G. Klochkov ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Acute Toxicity ◽  
Histone Deacetylases ◽  
Hydroxamic Acids ◽  
Water Soluble ◽  
Male Rat ◽  
Hybrid Male ◽  
Cyclic Hydroxamic Acids

Background: Anti-tumor effect of hydroxamic acid derivatives is largely connected with its properties as efficient inhibitors of histone deacetylases, and other metalloenzymes involved in carcinogenesis. Objective: The work was aimed to (i) determine the anti-tumor and chemosensitizing activity of the novel racemic spirocyclic hydroxamic acids using experimental drug sensitive leukemia P388 of mice, and (ii) determine the structure-activity relationships as metal chelating and HDAC inhibitory agents. Method: Outbreed male rat of 200-220 g weights were used in biochemical experiments. In vivo experiments were performed using the BDF1 hybrid male mice of 22-24 g weight. Lipid peroxidation, Fe (II) -chelating activity, HDAC fluorescent activity, anti-tumor and anti-metastatic activity, acute toxicity techniques were used in this study. Results: Chemosensitizing properties of water soluble cyclic hydroxamic acids (CHA) are evaluated using in vitro activities and in vivo methods and found significant results. These compounds possess iron (II) chelating properties, and slightly inhibit lipid peroxidation. CHA prepared from triacetonamine (1a-e) are more effective Fe (II) ions cheaters, as compared to CHA prepared from 1- methylpiperidone (2a-e). The histone deacetylase (HDAC) inhibitory activity, lipophilicity and acute toxicity were influenced by the length amino acids (size) (Glycine < Alanine < Valine < Leucine < Phenylalanine). All compounds bearing spiro-N-methylpiperidine ring (2a-e) are non-toxic up to 1250 mg/kg dose, while compounds bearing spiro-tetramethylpiperidine ring (1a-e) exhibit moderate toxicity which increases with increasing lipophility, but not excite at 400 mg/kg. Conclusion: It was shown that the use of combination of non-toxic doses of cisplatin (cPt) or cyclophosphamide with CHA in most cases result in the appearance of a considerable anti-tumor effect of cytostatics. The highest chemosensitizing activity with respect to leukemia Р388 is demonstrated by the CHA derivatives of Valine 1c or 2c.

In-vitro and in-vivo evaluation of antidiabetic activity of Withania coagulans extract

2019 ◽  
Vol 09 ◽  
Author(s):  
Tejas Patel ◽  
B.N. Suhagia
Keyword(s):  
Blood Glucose ◽  
Acute Toxicity ◽  
Aqueous Extract ◽  
Pancreatic Beta Cell ◽  
Toxicity Study ◽  
Acute Toxicity Study ◽  
Withania Coagulans ◽  
Study Results

Background: Diabetes mellitus is major issue to public health as its prevalence is rising day by day. Synthetic agents available for the diabetic treatment are expensive or produce undesirable side effect on chronic use and some of them are not suitable during pregnancy. Herbal medicines accepted widely due to side effects and low cost. Objective: The aim of present study was to evaluate the activity of Withania coagulans extract using In-vitro and In-vivo model. Methods: Different three types of Withania coagulans extract were prepared using aqueous (W1), Alcohol (W2) and hydro-alcoholic (50:50) mixture (W3). In-vitro Anti-diabetic activity of the all three extracts evaluated using RINm5F Pancreatic beta cells.Further, n-vivo anti-diabetic evaluation performed by administering 50 mg/kg (p.o) aqueous extract for 7 days in Streptozotocin (STZ)-induced mice. Body weight of the animals was also determined to perform acute toxicity study. Results: The results of in –vitro cell based study indicated that among all three extract, aqueous extract (W1) of Withania coagulans showed potential increase in inulin release. The EC50 of the W1 (249.6 µg/L) which is compared with standard (Glibenclamide) EC50. From the results of In-vitro study, W1 subjected for acute toxicity study and the acute toxicity study results indicated LD50 of 50mg/kg. Diabetic rats treated with W1 extract at oral dose of 50 mg/kg for 7 days showed 34.17% reduction in blood glucose in comparison to untreated diabetic (STZ-induced) rats. Blood glucose levels of Standard treated (Glibenclamide) and control untreated. Conclusion: In conclusion, results of pancreatic beta cell based study showed increase in insulin release by administration of extract. Further aqueous extract (W1) was potentially reduced blood glucose level in STZ induced diabetic mice.

Acute toxicity of C60–Cis-Pt nanocomplex in vivo

2021 ◽  
Author(s):  
Oksana Lynchak ◽  
Iryna Byelinska ◽  
Natalya Dziubenko ◽  
Halyna Kuznietsova ◽  
Olga Abramchuk ◽  
...  
Keyword(s):  
Acute Toxicity

scholarly journals PRESENCE OF A BASE LINE LEVEL OF SISTER CHROMATID EXCHANGES IN SOMATIC CELLS OF DROSOPHILA MELANOGASTER IN VIVO AND IN VITRO

Genetics ◽  
1982 ◽  
Vol 100 (2) ◽  
pp. 259-278
Author(s):  
Hideo Tsuji
Keyword(s):  
Drosophila Melanogaster ◽  
Sister Chromatid ◽  
Ganglion Cells ◽  
Sister Chromatid Exchanges ◽  
Base Line ◽  
Cell Cycles ◽  
Chromatid Exchanges ◽  
Third Instar Larvae

ABSTRACT Sister chromatid exchanges (SCEs) under in vivo and in vitro conditions were examined in ganglion cells of third-instar larvae of Drosophila melanogaster (Oregon-R). In the in vivo experiment, third-instar larvae were fed on synthetic media containing 5-bromo-2′-deoxyuridine (BrdUrd). After two cell cycles, ganglia were dissected and treated with colchicine. In the in vitro experiment, the ganglia were also incubated in media containing BrdUrd for two cell cycles, and treated with colchicine. SCEs were scored in metaphase stained with Hoechst 33258 plus Giemsa. The frequencies of SCEs stayed constant in the range of 25-150 vg/ml and 0.25-2.5 vg/ml of BrdUrd in vivo and in vitro, respectively. SCEs gradually increased at higher concentrations, strongly suggesting that at least a fraction of the detected SCEs are spontaneous. The constant levels of SCE frequency were estimated, on the average, at 0.103 per cell per two cell cycles for females and 0.101 for males in vivo and at 0.096 for females and 0.091 for males in vitro. No difference was found in the SCE frequency between sexes at any of the BrdUrd concentrations. The analysis for the distribution of SCEs within chromosomes revealed an extraordinarily high proportion of the SCEs at the junctions between euchromatin and heterochromatin; the remaining SCEs were preferentially localized in the euchromatic regions of the chromosomes and in the heterochromatic Y chromosome. These results were largely inconsistent with those of Gatti et al. (1979).

Sign in / Sign up

Export Citation Format

Share Document