Prediction of storability in soybean seeds through accelerated ageing technique [Glycine max (L.) Merill]

Author(s):  
Shivasharanappa . ◽  
S. Patil ◽  
S. R. Doddagoudar ◽  
Vijay Kumar Kurnalliker ◽  
Rakesh . ◽  
...  

An attempt was made to predict the relative storability of seeds of soybean varieties (JS-335 and DSB-21) through accelerated ageing technique in comparison with that of natural ageing where both the varieties had similar initial germination (93 %). The seeds were subjected to accelerated ageing conditions at 41 oC temperature and 95 ± 5 per cent relative humidity up to 180 hours. While, for natural ageing, the seeds were packed in cloth bag with the initial moisture of 9.5 per cent and stored under ambient conditions. The experimental results revealed that with the subsequent increase in accelerated ageing duration from 24 hours onwards up to 180 hours, the seed quality parameters viz., seed germination percentage, mean germination time, seedling vigour index and dehydrogenase enzyme activity decreased significantly. While the electrical conductivity increased significantly. When the accelerated ageing results were compared with that of natural ageing, accelerated ageing for 132 hours (67 % germination) was nearly equal to nine months of natural ageing (69 % germination) in case of JS-335 whereas in DSB-21, 144 hours of accelerated ageing (70 % germination) was equal to nine months of natural ageing (70 % germination). Hence, the accelerated ageing test can be used to predict the relative storability of soybean seeds. Hence, the seed producers can take decision regarding which seed lot can be stored for longer time and which one should be immediately sold to the market without any compromise in seed quality. It also helps in taking other management decisions.

2021 ◽  
Vol 13 (2) ◽  
pp. 735-743
Author(s):  
Umesh Hiremath ◽  
Basave Gowda ◽  
Lokesh G.Y . ◽  
B. S. Ganiger

The continuous rise in global population demands more food production under limited land resources accompanied by climate change. More oversupply of quality seeds to meet ever-growing food demand is a highly challenging task. Seeds with low vigour level limit the growth and yield of crops at varied field conditions. Hence, an experiment was conducted to develop a technique for the fast and homogeneous growth of kabuli chickpea seeds. The priming treatments evaluated in the present study enhanced the various seed quality parameters of kabuli chickpea significantly when compared to control. Hydro-priming for 4 hours (at 20°C) and air drying for 72 hours showed better results compared to rest of the treatments both in fresh seeds which recorded higher first count (66.38%), germination (92.50%) root length (11.85 cm), shoot length (6.16), mean seedling length (18.01), dry seedling weight (747 mg), seedling vigour index-I (1665), higher speed of emergence (0.3992) and old seeds with higher first count (61.88%), germination (74.13%) root length (8.15 cm), shoot length (5.85 cm), mean seedling length (14.00 cm), seedling dry weight (611 mg), seedling vigour index-I (1056), higher speed of emergence (0.3814). The aged seeds have recorded 29 % increase in germination percentage in contrast to 8 percent increases in fresh seeds. Kabuli chickpea seeds could be subjected to hydropriming, an affordable, alternative, and eco-friendly technique for improving seed and seedling vigour of kabuli chickpea.


Author(s):  
Ashok S Sajjan ◽  
Sujaya Waddinakatti ◽  
R B Jolli ◽  
Geetha D Goudar

The in vitro investigation was conducted during the kharif 2017 in the Department of Seed Science and Technology, College of Agriculture, Vijayapura. The experiment comprised of nine biopriming treatments along with control. The experiment was laid out in CRD with three replications. The seeds were subjected to in vitro studies and data was analysed statistically. The seed bioprimed with Rhizobium leguminosarum @ 20 per cent recorded higher seed germination percentage (90.50 %), shoot length (19.27 cm), root length (12.38 cm), seedling length (31.65 cm), seedling dry weight (373.0 mg), seedling vigour index I (2864), seedling vigour index II (33.75), dehydrogenase enzyme activity (1.010 OD value) and lower electrical conductivity (0.107 dS m-1) followed by Pseudomonas fluorescens @ 20 per cent, Fluorescent pseudomonad sp. @ 20 per cent as compared to control.


2017 ◽  
Vol 9 (1) ◽  
pp. 482-491
Author(s):  
Umesha Umesha ◽  
B. C. Channakeshava ◽  
K. Bhanuprakash ◽  
D. Nuthan ◽  
R. Siddaraju ◽  
...  

An experiment was conducted to investigate the influence of packaging materials and seed treatments on storability of cluster bean under ambient conditions of Bengaluru. The experiment consisted of six treatments viz. control (T1), bavistin @ 2 g kg-1 (T2), spinosad @ 0.04 ml/kg (T3), neem leaf powder @ 1:20 ratio (T4), Acorus calamus@ 10 g kg-1 (T5) and cow dung powder @ 10 g kg-1 (T6) and three packaging materials viz., cloth bag (C1) super grain bag (C2) and poly lined cloth bag (C3). Treated seed samples were stored in three containers under ambient storage conditions up to the duration of which seeds maintain minimum seed certification standards and samples were drawn at bimonthly intervals for ascertaining the seed quality parameters. The study suggested that seed treat-ment could be useful to prolong the storage life of cluster bean seeds. The seeds treated with spinosad (0.04 ml/kg) and stored in super-grain bag were better for maintenance of higher seed quality parameters [germination (80.00%), root length (11.70 cm), shoot length (13.60 cm), mean seedling dry weight (152 mg), seedling vigour index I and II (2024&12140) and TDH activity (1.224) with low electrical conductivity (0.368 dSm-1)] up to 18 months under ambient conditions of Bengaluru (room temperature). Super-grain bag proved to be better storage container with higher seed quality attributes viz., germination (72.38 %), seedling vigour index-I (1726), total dehydrogenase activity (1.201) and other seed quality parameters compared to cloth bag. The study suggested that use of appropriate packaging material and seed treatment could be useful to prolong the storage life of cluster bean seeds.


Author(s):  
J. B. Maruthi ◽  
R. Paramesh

A laboratory experiment was conducted at the Department of Seed Science and Technology, GKVK, University of Agricultural Sciences, Bangalore. The results revealed that, application of recommended dose of NPK (30:80: 37.5 kg ha-1) + Recommended dose of FYM (10 t ha-1) +Brady rhizobium (250 g ha-1)+PSB (250 g ha-1) significantly improved the seed quality parameters viz., 100 seed weight (35.51g), seed size (0.76 cm<sup>3</sup>), seed density (1.55 g/cc), total dehydrogenase activity (4.80), germination (92.00 %), seedling length (30.69 cm), seedling dry weight (90.75 mg), seedling vigour index- I and II (2820 and 836 respectively), protein content (36.30 %) and field emergence (89.33 %) with lowest electrical conductivity (0.49 mScm-1) as compared to control and other treatments. Further, artificially aged seeds through accelerated ageing method also recorded higher seed quality parameters in the same treatment at the end of six days of ageing period.


2016 ◽  
Vol 8 (3) ◽  
pp. 1638-1642
Author(s):  
Gajendra Khidrapure ◽  
S. N. Vasudevan ◽  
S. R. Doddagoudar ◽  
A. G. Sreenivas ◽  
Satyanarayana Rao

The present study was conducted to know the storage potential of organically produced paddy seeds in the Department of Seed Science and Technology, University of Agricultural Sciences, Raichur. The seed lot were divided into two parts, one part was stored in cloth bag for a period of 12 months under ambient conditions. At thesame time another set of seeds were subjected to accelerated ageing at 42 + 10C temperature and 90 per cent relative humidity (RH) for a period of 0-12 days. Among the ageing methods, artificially aged seeds showed drastic decreases in seed quality as compared to natural ageing. Among the treatments T9 (37.5 % FYM + 37.5 % vermicompost + 25 % neem cake + foliar spray of panchagavya on 30, 60, 90 and 120 DAT) recorded significantly highest seed quality parameters viz., seed germination (97.81 %), seedling length (29.42 cm) and SVI (2878) at initial stage in both the method of aging and at the end of storage period; seed germination (71.23 and 87.33 %), seedling length (19.66 and 27.00 cm) and SVI (1400 and 2358) in accelerated ageing (AA) and natural ageing (NA) respectively, whereas, lowest in control (Inorganic treatment). The seed quality parameters of four days of AA were similar to that of six months of NA. Hence, storability of organically produced paddy seeds were better as compared to inorganic seeds and it can be predicted that four days of AA is equal to six months of NA. The information generated will be useful in retention or disposal of a particular variety or seed lot.


2017 ◽  
Vol 9 (4) ◽  
pp. 2338-2343
Author(s):  
Arpana D. Vaja ◽  
J. B. Patel ◽  
R.N. Daki ◽  
Shital A. Chauhan

The present investigation on effect of nitrogen [N0 (Control), N1 (50 kg N/ha), N2 (100 kg N/ha), and N3 (150 kg N/ ha)] and plant growth regulators [G0 (Control), G1 (50 ppm GA3), G2 (50 ppm NAA) and G3 (500 ppm Cycocel)] on seed yield per plant and seed quality parameters in brinjal (Solanum melongena L.) cv. GJB 3 was carried out at the Instructional Farm, Department of Agronomy, Junagadh Agricultural University, Junagadh during kharif 2015-16. The experiment was laid out in field as per randomized block design (Factorial) with three replications. The seed harvested from 16 different treatments combinations replicated thrice from the field were analyzed in the laboratory following completely randomized design (factorial) for various seed quality parameters. Application of nitrogen @ 150 kg N/ha recorded significantly (P<0.05) highest seed yield per plant, shoot fresh weight, root dry weight, shoot dry weight and vigour index – 2 (mass), while application of 100 kg N/ha resulted in significantly (P<0.05) highest germination percentage, root length, shoot length, root fresh weight and vigour index – 1 (length). Application of GA3 at 50 ppm recorded significantly the highest seed yield per plant, germination percentage, root length, shoot length, root fresh weight, shoot fresh weight, root dry weight, shoot dry weight, vigour index – 1 (length) and vigour index – 2 (mass). Among the 16 different treatment combinations, nitrogen @ 150 kg/ha and GA3 50 ppm noted the maximum seed yield per plant, shoot dry weight and vigour index – 2 (mass), while significantly the maxi-mum germination percentage, root length, shoot length, root fresh weight, shoot fresh weight and vigour index – 1 (length) were registered in treatment combination nitrogen 100 kg N/ha and GA3 @ 50 ppm. Therefore, it is advised that application of 100-150 kg of N/ha as a nitrogenous fertilizer and spray GA3 @ 50 ppm (G1) at 45 days after transplanting helps in increasing the seed yield per plant and seed quality parameters in brinjal seed production.


Author(s):  
Shivasharanappa S. Patil ◽  
Ashok S. Sajjan ◽  
N.K. Biradarpatil ◽  
P.U. Krishnaraj ◽  
I.S. Katageri

Background: Accelerated ageing is a method to assess seed storage performance by exposing the seeds to higher temperature and relative humidity to make seeds lose its vigour and viability quickly. The seed physiological parameters are then compared with the natural ageing to derive certain conclusions. But, the molecular pattern of seed deterioration under accelerated ageing varies compared to that of natural ageing.Methods: Chickpea, seed lots of variety JG-11 and Annigeri-1 were subjected to natural ageing in cloth bag with nine per cent initial seed moisture under ambient conditions. Simultaneously, representative sample of same seed lot were also aged at 41oC and 95±2% humidity up to 120 hours.Result: Both the ageing methods had significant negative effect on seed physiological and biochemical quality parameters. The germination potential of seeds tested after 48 hours of accelerated ageing (83.5%) was equal 10 months of natural ageing (85%) in JG-11, while also in Annigeri-1, 48 hours of accelerated ageing (78%) was nearly equal to 10 months of natural ageing (80%). The protein expression analyzed through 2D-PAGE at similar germination potential brought out by two independent ageing methods showed higher protein down regulation ratio (3.4) in accelerated ageing than in natural ageing (3.2) unveiling its rapidity in the seed deterioration process. Therefore, accelerated ageing can be used for predicting chickpea seed longevity.


2016 ◽  
Vol 8 (1) ◽  
pp. 340-345
Author(s):  
N. Amruta ◽  
P. J. Devaraju ◽  
Mangalagowri Mangalagowri ◽  
S. P. Kiran ◽  
H. P. Ranjitha ◽  
...  

A field experiment was conducted to assess the response of nutrient levels and spacing on seed quality attributes of black gram cv. LBG-625 (Rashmi). Experimental results revealed that fertilizer application of 50:100:100 + Black gram rhizobia (250 g ha-1) + PSB- Bacillus megaterium (250 g ha-1) recorded highest test weight (39.27 g), germination (90.60%), root length (15.77 cm), shoot length (13.43 cm), mean seedling length (29.20 cm), mean seedling dry weight (57.99 mg), seedling vigour index-I (2656), seedling vigour index-II (525), total dehydrogenase activity (0.998), protein content (23.16%), field emergence (86.56 %) lowest electrical conductivity (0.813 dSm-1) were superior over other fertilizer treatments. Among the planting geometry 60 x 10 cm recorded more germination per cent (90.48 %) compared to 45 x 10 cm (87.64 %) and 30 x 10 cm (86.91 %). Interaction of nutrient levels and spacing through the application of 50:100:100 + black gram rhizobia (250 g ha-1) + PSB- B. megaterium (250 g ha-1) with planting geometry 60 x 10 cm recorded more mean seedling length (34.40 cm), mean seedling dry weight (58.30 mg),and field emergence (90.24 %) lowest electrical conductivity (0.776 dSm-1) compared to control. The application of 50:100:100 + Black gram rhizobia (250 g ha-1) + PSB- B. megaterium (250 g ha-1) with planting geometry 60 x 10 cm were considered as seed quality improvement approach in blackgram, therefore conjunctive use of inorganic fertilizers and biofertilizer may be suggested for higher seed quality parameters along with overall betterment of crop.


2019 ◽  
Vol 11 (2) ◽  
pp. 394-397
Author(s):  
Navitha P. ◽  
Sujatha K. ◽  
Beaulah A.

An experiment was carried out at the Department of Seed Science and Technology, Agricultural College and Research Institute, Madurai during 2018 to find out the effect of fruit size on physiological seed quality of cucumber. Variation in fruit size of cucumber results in poor quality seeds. In order to overcome this obstacle fruit grading was done based on weight of fruit to obtain good quality seeds. Harvested fruits of cucumber (Cucumis sativus) were categorized based on the weight into three different groups viz., Big (2.41kg), medium (1.66kg) and small (1.28kg). Observations on fruit and seed quality parameters were recorded. The results revealed that medium sized fruits recorded higher values compared to big and small sized fruits. The number of seeds/fruit recorded higher in medium sized fruit (935 numbers) followed by small (896 numbers) and big (876 numbers) sized fruits. The big, medium and small fruits were recovered to 1.52 %, 1.06% and 0.58% seeds respectively. The physiological quality characters measured in terms of seed germination revealed that seeds of medium sized fruits were recorded higher (80%) followed by seeds of big (82%) and small (65%). The seedling vigour measured through root (17.08cm) and shoot length (14.45cm), dry matter production (0.85g 10 seedlings-1) and vigour index (2522) also proved the superiority in medium sized fruits.


2014 ◽  
Vol 3 (3) ◽  
pp. 149-153 ◽  
Author(s):  
Venuturla Bharathi ◽  
Kilaru K. Durga ◽  
Madugula S. Rani

Planting healthy seeds and other inputs have an impact on seed quality, fungal invasion deteriorates the seed quality in soil and storage. Studies on chickpea carried out at Seed Research and Technology Center, Rajendranagar, Hyderabad during spring 2010-11 2011-12 revealed that benomyl was found effective followed by Pseudomonas fluorescens in the inhibition of mycelial growth of Fusarium solani. Maximum germination percentage was recorded with Benomyl (Benomyl 500 WP) @ 2 g/kg seed (treated check) and Tebuconazole @ 1 ml/ kg seed (95 per cent) followed by treatment with P. fluorescens @ 10 g/kg seed along with soil application of P. fluorescens @ 3 kg/acre (94 per cent). Maximization of growth parameters like root length, shoot length and total seedling length were observed with Benomyl @ 2 g/ kg seed  as 17.0 cm, 10.3 cm and 27.3 cm, respectively. Considering seedling vigour index as an important seed quality character, P. fluorescens and Benomyl @ 2 g/kg seed recorded high seedling vigor index. The per cent recovery of infested seeds was found to be low with treated seeds when compared to the control.


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