scholarly journals Prediction of storability of organically produced paddy seeds through natural and accelerated ageing techniques

2016 ◽  
Vol 8 (3) ◽  
pp. 1638-1642
Author(s):  
Gajendra Khidrapure ◽  
S. N. Vasudevan ◽  
S. R. Doddagoudar ◽  
A. G. Sreenivas ◽  
Satyanarayana Rao
Keyword(s):  
Seed Germination ◽  
Seed Quality ◽  
Foliar Spray ◽  
Storage Period ◽  
Quality Parameters ◽  
Accelerated Ageing ◽  
Natural Ageing ◽  
Ambient Conditions ◽  
Seedling Length ◽  
Cent Relative Humidity

The present study was conducted to know the storage potential of organically produced paddy seeds in the Department of Seed Science and Technology, University of Agricultural Sciences, Raichur. The seed lot were divided into two parts, one part was stored in cloth bag for a period of 12 months under ambient conditions. At thesame time another set of seeds were subjected to accelerated ageing at 42 + 10C temperature and 90 per cent relative humidity (RH) for a period of 0-12 days. Among the ageing methods, artificially aged seeds showed drastic decreases in seed quality as compared to natural ageing. Among the treatments T9 (37.5 % FYM + 37.5 % vermicompost + 25 % neem cake + foliar spray of panchagavya on 30, 60, 90 and 120 DAT) recorded significantly highest seed quality parameters viz., seed germination (97.81 %), seedling length (29.42 cm) and SVI (2878) at initial stage in both the method of aging and at the end of storage period; seed germination (71.23 and 87.33 %), seedling length (19.66 and 27.00 cm) and SVI (1400 and 2358) in accelerated ageing (AA) and natural ageing (NA) respectively, whereas, lowest in control (Inorganic treatment). The seed quality parameters of four days of AA were similar to that of six months of NA. Hence, storability of organically produced paddy seeds were better as compared to inorganic seeds and it can be predicted that four days of AA is equal to six months of NA. The information generated will be useful in retention or disposal of a particular variety or seed lot.

Prediction of storability in soybean seeds through accelerated ageing technique [Glycine max (L.) Merill]

2017 ◽  
Author(s):  
Shivasharanappa . ◽  
S. Patil ◽  
S. R. Doddagoudar ◽  
Vijay Kumar Kurnalliker ◽  
Rakesh . ◽  
...  
Keyword(s):  
Seed Quality ◽  
Germination Percentage ◽  
Quality Parameters ◽  
Accelerated Ageing ◽  
Soybean Seeds ◽  
Natural Ageing ◽  
Ambient Conditions ◽  
Seedling Vigour ◽  
Subsequent Increase ◽  
Vigour Index

An attempt was made to predict the relative storability of seeds of soybean varieties (JS-335 and DSB-21) through accelerated ageing technique in comparison with that of natural ageing where both the varieties had similar initial germination (93 %). The seeds were subjected to accelerated ageing conditions at 41 oC temperature and 95 ± 5 per cent relative humidity up to 180 hours. While, for natural ageing, the seeds were packed in cloth bag with the initial moisture of 9.5 per cent and stored under ambient conditions. The experimental results revealed that with the subsequent increase in accelerated ageing duration from 24 hours onwards up to 180 hours, the seed quality parameters viz., seed germination percentage, mean germination time, seedling vigour index and dehydrogenase enzyme activity decreased significantly. While the electrical conductivity increased significantly. When the accelerated ageing results were compared with that of natural ageing, accelerated ageing for 132 hours (67 % germination) was nearly equal to nine months of natural ageing (69 % germination) in case of JS-335 whereas in DSB-21, 144 hours of accelerated ageing (70 % germination) was equal to nine months of natural ageing (70 % germination). Hence, the accelerated ageing test can be used to predict the relative storability of soybean seeds. Hence, the seed producers can take decision regarding which seed lot can be stored for longer time and which one should be immediately sold to the market without any compromise in seed quality. It also helps in taking other management decisions.

scholarly journals Quality Deterioration of Commercial Bean (Phaseolus vulgaris) Seeds Stored under Contrasting Environmental Conditions in Sri Lanka

2020 ◽  
Vol 8 (1) ◽  
pp. 33-44
Author(s):  
R.A.I.S. Ariyarathna ◽  
S.L. Weerasena ◽  
C.K. Beneragama
Keyword(s):  
Sri Lanka ◽  
Low Temperature ◽  
Environmental Conditions ◽  
Seed Quality ◽  
Storage Period ◽  
Fluorescence Analysis ◽  
Quality Parameters ◽  
Full Potential ◽  
Ambient Conditions ◽  
Field Emergence

Bean is a popular vegetable grown in most parts of Sri Lanka. Farmers and home gardeners purchase seeds in packeted form retail outlets. However, quality problems are frequently reported, especially on low field germination. Quality parameters namely, percentage germination, field emergence, seedling length, vigour index and chlorophyll fluorescence analysis were used to determine the effect of different storage environmental conditions on seed longevity of two bean varieties during a two-year storage period, 2016 to 2018. Seeds in triple-laminated packets stored in four contrasting conditions; low temperature storage conditions (Tem. 5oC, 65% RH); three geo-environmental conditions namely, Gannoruwa (mid country wet zone), Kundasale (mid country intermediate zone), and Mahailuppallama (low country dry zone) showed varied behavior in seed quality parameters respective of the variety. It was apparent that germination test alone does not represent the full potential of field emergence. Results of the present study revealed that when field emergence and vigour are also taken in to account, both varieties of bean seeds can be stored under ambient conditions at Gannoruwa, Kundasale or MahaIlluppallama for a maximum period of 12 months, without compromising the seed quality. The seeds stored under low temperature conditions (i.e. 5 °C), maintained the seed germination over 75% for a period of 24 months.  

Accelerated Ageing Mediated Seed Longevity Prediction and Assessment of Seed Deterioration Pattern through 2D-Gel Electrophoresis in Chickpea (Cicer arietinum L.)

2021 ◽  
Author(s):  
Shivasharanappa S. Patil ◽  
Ashok S. Sajjan ◽  
N.K. Biradarpatil ◽  
P.U. Krishnaraj ◽  
I.S. Katageri
Keyword(s):  
Seed Storage ◽  
Quality Parameters ◽  
Seed Longevity ◽  
Accelerated Ageing ◽  
Natural Ageing ◽  
Ambient Conditions ◽  
Seed Deterioration ◽  
Germination Potential ◽  
Deterioration Process ◽  
Ageing Methods

Background: Accelerated ageing is a method to assess seed storage performance by exposing the seeds to higher temperature and relative humidity to make seeds lose its vigour and viability quickly. The seed physiological parameters are then compared with the natural ageing to derive certain conclusions. But, the molecular pattern of seed deterioration under accelerated ageing varies compared to that of natural ageing.Methods: Chickpea, seed lots of variety JG-11 and Annigeri-1 were subjected to natural ageing in cloth bag with nine per cent initial seed moisture under ambient conditions. Simultaneously, representative sample of same seed lot were also aged at 41oC and 95±2% humidity up to 120 hours.Result: Both the ageing methods had significant negative effect on seed physiological and biochemical quality parameters. The germination potential of seeds tested after 48 hours of accelerated ageing (83.5%) was equal 10 months of natural ageing (85%) in JG-11, while also in Annigeri-1, 48 hours of accelerated ageing (78%) was nearly equal to 10 months of natural ageing (80%). The protein expression analyzed through 2D-PAGE at similar germination potential brought out by two independent ageing methods showed higher protein down regulation ratio (3.4) in accelerated ageing than in natural ageing (3.2) unveiling its rapidity in the seed deterioration process. Therefore, accelerated ageing can be used for predicting chickpea seed longevity.

Influence of Seed Biopriming with Microbial Inoculants on Seed Quality Parameters in Soybean [Glycine max (L.) Merril]

2020 ◽  
Author(s):  
Pramod Sharma ◽  
Arun Bhatt ◽  
Rajesh Kanwar
Keyword(s):  
Seed Germination ◽  
Seed Quality ◽  
Dry Weight ◽  
Positive Influence ◽  
Quality Parameters ◽  
Quality Parameter ◽  
Seedling Vigour ◽  
Seed Vigour ◽  
Seedling Length ◽  
Vigour Index

The present investigation was carried out in the Department of Seed Science and Technology, College of Forestry, Ranichauri, Tehri Garhwal, Uttarakhand during 2015. A laboratory experiment was conducted to find out the influence of bio-priming on seed germination and seedling vigour. Seed germination was reported maximum for Psf-173 (93.66%) while minimum was reported for control (65.66%). Seed vigour parameter viz. root length (11.01 cm), shoot length (7.96 cm), seedling length (18.98 cm), fresh weight (8.00 g) and dry weight (1.31 g), vigour index I (1666.80) and vigour index II (115.56) was found higher for PSB treatment. Shilajeet variety had recorded significant higher values for most of the germination and vigour parameters. Shilajeet bio-primed with PSB attained higher values for majority of the quality parameters. Under laboratory condition results suggested that seed germination and seed vigour were greatly affected by different bio-agents. Among all the bio-agents, PSB showed positive influence on most of the quality parameter. However, other bio-agents had also showed significant effect over control.

scholarly journals Deleterious Effects of Storage Environmental Conditions on The Seed Quality of Two Varieties of Tomato (Solanum lycopersicum L.) Stored in Triple Laminated Aluminum Packing in Sri Lanka

2020 ◽  
Vol 8 (4) ◽  
pp. 437-447
Author(s):  
R.A.I.S. Ariyarathna ◽  
S. L. Weerasena ◽  
C. K. Beneragama
Keyword(s):  
Sri Lanka ◽  
Seed Germination ◽  
Environmental Conditions ◽  
Seed Quality ◽  
Quality Parameters ◽  
Vegetable Crops ◽  
Ambient Conditions ◽  
Tomato Seeds ◽  
Vigour Index ◽  
Field Emergence

Tomato is one of the most commonly growing vegetable crops among the farmers in Sri Lanka. Standard laboratory germination of tomato seeds fulfills the regulatory requirements of seed marketing. However, poor field performance is an overwhelming problem to farmers. Present study was focused on the longevity of two varieties of tomato seeds as affected by their quality characters (percentage germination, moisture, field emergence and vigour index, seed protein and carbohydrate contents) including seed health (exposure of Clavibacter michiganensis subsp. Michiganensis (CMMV), Spotted wilt virus (TSWV) and Leaf curly top virus (LCTV)) under four different storage environmental conditions during a one-year long storage study. Seeds were packed in triple-laminated aluminum packets (TLA) and stored in low temperature storage conditions (17±1 °C and 65% RH) and in ambient conditions in Gannoruwa, Kundasale, and MahaIlluppallama that represent the three agro-ecologically zones; Mid Country Wet Zone (MCWZ), Mid Country Intermediate Zone (MCIZ) and Low Country Dry Zone (LCDZ) respectively. Seed quality parameters displayed varied responses depending on the variety. Physiological changes that occur in seeds due to temperature, RH, changes in proteins and carbohydrate content, CMMV and TSWV during storage were manifested as reduction in seed germination, field emergence and vigour index. However, none of the seedling carried LCTV and therefore its effect on seed quality parameters could not be assessed. The seeds could be stored for ca. 29 months at low and constant temperatures and RH conditions without compromising local seed germination standards compared to ambient storage in the three environments. CMMV and TSWV detected in all seed samples indicated potential threats to tomato farmers in Sri Lanka.  

scholarly journals Effect of Storage Conditions on Seed Viability of China Aster [Callistephus chinensis (L.) Nees]

2021 ◽  
Vol 10 (10) ◽  
pp. 385-401
Author(s):  
B. Vaidya P. B. Sable ◽  
V. B. Patil
Keyword(s):  
Seed Germination ◽  
Field Condition ◽  
Seed Quality ◽  
Seed Viability ◽  
Storage Conditions ◽  
Quality Parameters ◽  
Root Weight ◽  
Ambient Conditions ◽  
China Aster ◽  
Shoot Weight

The study revealed that China aster seeds lose viability quickly if stored under ambient conditions. At the end of 12th months, seeds stored at refrigerated condition (5-70C) still maintained high seed germination (64.00%) at field condition well above the minimum seed certification standards. However, in case of ambient storage very drastic reduction in seed germination was observed. It was 32.43 % for first months and reduced to 14.95 % for second month by declining 53.90% as compared to first month and thereafter no germination was recorded at field condition. Results from the interaction effect indicated that previously stored seeds of China aster for six months in aluminium foil and kept under refrigerated condition recorded higher seed quality parameters viz. percent seed germination at field (92.67 and 72.00%), percent survival of seedlings (89.33 and 70.67%), height of seedling (11.53 and 8.60 cm), shoot length (7.00 and 5.10 cm), root length (4.53 and 3.50 cm), shoot weight (0.42 and 0.33 g), root weight (0.040 and 0.027 g), root:shoot ratio (0.095 and 0.082) and percent seed germination at laboratory (97.33 and 76.00%) for first and sixth month, respectively.

Effect of integrated nutrient management on seed quality of vegetable soybean [Glycine max (L.) merrill] cv. Karune

2016 ◽  
Author(s):  
J. B. Maruthi ◽  
R. Paramesh
Keyword(s):  
Nutrient Management ◽  
Seed Quality ◽  
Dry Weight ◽  
Quality Parameters ◽  
Recommended Dose ◽  
Accelerated Ageing ◽  
Seedling Vigour ◽  
Seedling Length ◽  
Vigour Index ◽  
Ageing Period

A laboratory experiment was conducted at the Department of Seed Science and Technology, GKVK, University of Agricultural Sciences, Bangalore. The results revealed that, application of recommended dose of NPK (30:80: 37.5 kg ha-1) + Recommended dose of FYM (10 t ha-1) +Brady rhizobium (250 g ha-1)+PSB (250 g ha-1) significantly improved the seed quality parameters viz., 100 seed weight (35.51g), seed size (0.76 cm<sup>3</sup>), seed density (1.55 g/cc), total dehydrogenase activity (4.80), germination (92.00 %), seedling length (30.69 cm), seedling dry weight (90.75 mg), seedling vigour index- I and II (2820 and 836 respectively), protein content (36.30 %) and field emergence (89.33 %) with lowest electrical conductivity (0.49 mScm-1) as compared to control and other treatments. Further, artificially aged seeds through accelerated ageing method also recorded higher seed quality parameters in the same treatment at the end of six days of ageing period.

scholarly journals Role of seed-Zn content on seed longevity of pigeonpea genotypes

2018 ◽  
Author(s):  
D. Hanumanthappa ◽  
S.N. Vasudevan ◽  
N.M. Shakuntala ◽  
M. Muniswamy ◽  
B. Kisan ◽  
...  
Keyword(s):  
Seed Quality ◽  
Storage Period ◽  
Quality Parameters ◽  
Seed Longevity ◽  
Seedling Vigour ◽  
Seedling Length ◽  
Zn Content ◽  
Dehydrogenase Enzyme ◽  
Vigour Index

Fifteen pigeonpea genotypes were selected to establish the role of seed-Zn content in maintaining seed longevity of pigeonpea genotypes. The present study revealed clear genotypic variability with respect to storability among different pigeonpea genotypes. The genotype with highest seed-Zn content (30.81 ppm) proved as good storer by recording highest seed quality parameters viz., seed germination (84.57 %), seedling length (27.67 cm), seedling vigour index (1303), speed of germination (23.29 %), dehydrogenase enzyme activity (0.551 OD value), a-amylase activity (28.46 mm) with lowest electrical conductivity (1.04 µS/cm) and moisture content (7.29 %) at the end of twelve months of storage period. Whereas, genotypes with low seed-Zn content showed lowest seed quality parameters.

scholarly journals Effect of Aspergillus spp. on Seed Quality Characters of Groundnut

2021 ◽  
Author(s):  
N. Aiswarya ◽  
S.L. Bhattiprolu ◽  
K. Bayyapu Reddy ◽  
G. Rama Rao
Keyword(s):  
Seed Germination ◽  
Seed Quality ◽  
Dry Weight ◽  
Quality Parameters ◽  
Aflatoxin Contamination ◽  
Consumer Health ◽  
Seedling Vigour ◽  
Seedling Length ◽  
Vigour Index ◽  
The Impact

Background: Aspergillus niger and A. flavus cause rotting, damping-off, loss in germination and toxification of oil seeds. Aspergillus is a common mold in tropical and Sub-tropical countries and causes aflatoxin contamination. A. flavus and A. niger were responsible for storage rot of groundnut. In addition to storage rot, aflatoxin contamination also leads to adverse effects on consumer health. Therefore, present study was conducted to estimate the impact of both A. niger and A. flavus on seed quality parameters of groundnut. Methods: Effect of major seed borne fungi A.niger and A. flavus on seed quality characters of thirteen different genotypes of groundnut was observed in the present study. All the seedling quality characters were measured as per ISTA guidelines. Result: On an average A. niger caused 25.14, 25.18, 43.99, 26.20 and 44.75 per cent reduction in seed germination, seedling length, seedling vigour index I, seedling dry weight and seedling vigour index II, respectively, over the control whereas A. flavus caused average per cent reduction of 22.38, 24.23, 41.18, 23.92 and 40.95 in seed germination, seedling length, seedling vigour index I, seedling dry weight and seedling vigour index II, respectively, over the control.

scholarly journals Development of priming technology for enhanced planting value of seeds in kabuli chickpea (Cicer arietinum L.)

2021 ◽  
Vol 13 (2) ◽  
pp. 735-743
Author(s):  
Umesh Hiremath ◽  
Basave Gowda ◽  
Lokesh G.Y . ◽  
B. S. Ganiger
Keyword(s):  
Root Length ◽  
Seed Quality ◽  
Dry Weight ◽  
Germination Percentage ◽  
Quality Parameters ◽  
Shoot Length ◽  
Growth And Yield ◽  
Seedling Vigour ◽  
Seedling Length ◽  
Vigour Index

The continuous rise in global population demands more food production under limited land resources accompanied by climate change. More oversupply of quality seeds to meet ever-growing food demand is a highly challenging task. Seeds with low vigour level limit the growth and yield of crops at varied field conditions. Hence, an experiment was conducted to develop a technique for the fast and homogeneous growth of kabuli chickpea seeds. The priming treatments evaluated in the present study enhanced the various seed quality parameters of kabuli chickpea significantly when compared to control. Hydro-priming for 4 hours (at 20°C) and air drying for 72 hours showed better results compared to rest of the treatments both in fresh seeds which recorded higher first count (66.38%), germination (92.50%) root length (11.85 cm), shoot length (6.16), mean seedling length (18.01), dry seedling weight (747 mg), seedling vigour index-I (1665), higher speed of emergence (0.3992) and old seeds with higher first count (61.88%), germination (74.13%) root length (8.15 cm), shoot length (5.85 cm), mean seedling length (14.00 cm), seedling dry weight (611 mg), seedling vigour index-I (1056), higher speed of emergence (0.3814). The aged seeds have recorded 29 % increase in germination percentage in contrast to 8 percent increases in fresh seeds. Kabuli chickpea seeds could be subjected to hydropriming, an affordable, alternative, and eco-friendly technique for improving seed and seedling vigour of kabuli chickpea.

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