Characterization of rhizobia from root nodule and rhizosphere of Vicia faba in Algeria

2018 ◽  
Author(s):  
Benselama Amela ◽  
Tellah Sihem ◽  
Ourem Faiza ◽  
Ounane Sidi Mohamed
Keyword(s):  
Nitrogen Fixation ◽  
Vicia Faba ◽  
Congo Red ◽  
Total Carbon ◽  
Leguminous Plant ◽  
Available Phosphorus ◽  
Good Growth ◽  
Biochemical Tests ◽  
Poor Growth

Nitrogen fixation resulting from mutual symbiosis of rhizobia and cultivated legume plants is therefore critical to food security as it directly affects agricultural production. Biological Nitrogen Fixation (BNF) can be an important factor in sustainable agriculture. A collection of 20 isolates from fresh Nodules of the legume plant Vicia faba was isolated from five soil of Algeria. The soil from each region has undergone physical-chemical analysis : (granulometry, total carbon, organic matter, pH, and electrical conductivity, determination of available phosphorus and determination of total nitrogen). A media viz., Glucose-Peptone Agar (GPA), Congo red, Yeast Mannitol Agar (YMA) containing 2% NaCl were employed to make presumptive decisions on the recognition and classification of the isolated bacterial strains. All the isolates were found with poor absorption of dye Congo red and little or no growth on the media of GPA and without altering the pH. Almost all of the isolates exhibit good growth on 2% NaCl, poor growth on GPA, thus confirming the rhizobia. After biochemical tests like catalase test and citrate utilization test isolates were confirmed. The presence of rhizobia on root nodules of leguminous plant.

scholarly journals Characterization of Rhizobia from Root Nodule and Rhizosphere of Lablab purpureus and Vigna sinensis in Bangladesh

2017 ◽  
Vol 5 (1) ◽  
pp. 14 ◽  
Author(s):  
Safirun Pervin ◽  
Bushra Jannat ◽  
Sohana Al Sanjee
Keyword(s):  
Nitrogen Fixation ◽  
Congo Red ◽  
Root Nodules ◽  
Leguminous Plant ◽  
Lablab Purpureus ◽  
Bacterial Strains ◽  
Vigna Sinensis ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Poor Growth

Nitrogen fixation resulting from mutual symbiosis of rhizobia and cultivated legume plants is therefore critical to food security as it directly affects agricultural production. Biological Nitrogen Fixation (BNF) can be an important factor in sustainable agriculture.The isolation and identification of different slow growing and fast growing rhizobial strains from the nodules of two leguminous plant species. Symbiotic nitrogen fixing Rhizobium spp. was isolated from (Lablab purpureus and Vigna sinensis). Nodules samples were collected from plants growing in different Districts of Bangladesh and the Glucose-Peptone Agar (GPA), Congo red, Yeast Mannitol Agar (YMA) containing 2% NaCl were employed to make presumptive decisions on the recognition and classification of the isolated bacterial strains. All the isolates were found with poor absorption of dye Congo red and little or no growth on the media of GPA and without altering the pH. Almost all of the isolates exhibit growth on 2% NaCl, poor growth on GPA, thus confirming the rhizobia. After biochemical tests like catalase test and citrate utilization test isolates were confirmed as Rhizobia. The presence of rhizobia on root nodules of leguminous Plant. Not only the leguminous Plant but also the rhizosphere contains rhizobia which help in soil fertilization.

scholarly journals Phosphorus deficiency enhances water deficit impact on some morphological and physiological traits in four faba bean (Vicia faba L.) varieties

2020 ◽  
Author(s):  
Khawla Oukaltouma ◽  
Ahmed El Moukhtari ◽  
Yahya Lahrizi ◽  
Mohammed Mouradi ◽  
Mohamed Farissi ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Water Deficit ◽  
Vicia Faba ◽  
Faba Bean ◽  
Phosphorus Deficiency ◽  
Leaf Water ◽  
Nutrient Concentrations ◽  
Available Phosphorus ◽  
High Concentration ◽  
Tolerant Variety

Moroccan soils, generally present low available phosphorus (P) levels which occur in almost all arid and semi-arid regions. Faba bean is one of the most significant crops in Morocco and is influenced by these constraints that affect its nutrient uptake and nitrogen fixation capacity and hence plants development. Therefore, we evaluated the response of four Vicia faba varieties –Aguadulce (Ag), Alfia (Al), Luz de Otono (LO) and Reina Mora (RM)-, grown under Biological Nitrogen Fixation (BNF) to water deficit and two phosphorus levels. The trial was conducted under greenhouse conditions and water stress was induced by keeping pots at 40% substrate field capacity (FC) versus 80% FC for the controls while phosphorus deficiency treatment consisted in the application of 25 µmol P plant− 1 week−1 versus 125 µmol P plant− 1 week−1 for sufficient P treatment. The results revealed a significant effect of water deficit and phosphorus deficiency either alone or combined on plants dry weights, leaf water parameters and nutrient concentrations. However sufficient phosphorus supply mitigated the adverse effects of water deficit on faba bean. We noticed significant differences between the studied varieties. Ag showed high performance concerning dry weights (1.25 g and 1.88 g plant-1 respectively for shoot and root) and high concentration of nitrogen N (4.7%) and P (0.27 mg g-1 DW) and was then qualified as the most tolerant variety to water deficit combined with P limitation. While RM was the least tolerant variety, as it showed the lowest dry weights (0.51 g and 1.4 g plant-1 respectively for shoot and root) and concentration (2.74% and 0.19 mg g-1 DW respectively for N and P). The tolerance was related to the ability to ensure efficient osmoregulation by glycine betaine accumulation, to keep leaf water balance and cell membrane stability that contribute together with adequate symbiotic nitrogen fixation to plant growth performance under combined stresses.

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