scholarly journals MOLECULAR AND BIOLOGICAL CHARACTERISTICS OF VACCINARY ERA-CB 20M OF RABIES VIRUS

2018 ◽  
Vol 63 (5) ◽  
pp. 224-232
Author(s):  
M. A. Losich ◽  
O. N. Zaykova ◽  
I. V. Nepoklonova ◽  
T. V. Grebennikova ◽  
O. A. Verkhovsky ◽  
...  
Keyword(s):  
Cell Culture ◽  
Phylogenetic Analysis ◽  
Cell Lines ◽  
Rabies Virus ◽  
Molecular Analysis ◽  
Reference Strain ◽  
Biological Characteristics ◽  
Gene Fragment ◽  
Medical Sciences ◽  
Rabies Virus Strain

The molecular and biological characteristics of the vaccine against rabies virus strain ERA-CB 20M obtained by the Russian rabiologist, doctor of medical sciences S.V. Gribencha by adapting and cloning the strain ERA and SAD in a transplantable BHK-21 C13 cell culture are presented. The spectrum of the most sensitive strain of rabies ERA-CB 20M cell lines was determined and the level of glycoprotein was quantitatively determined. Primary nucleotide sequences of fragments of the genome of the strain ERA-CB 20M (genes N and G) were obtained and phylogenetic analysis was carried out. Molecular analysis showed that this strain belongs to the group of vaccine strains SAD1. When compared with the reference strain SAD1, 10% of the nucleotide differences were revealed in the gene fragment N; 15%, in the gene fragment G.

Whole Genome Sequencing and Phylogenetic Analysis of the Rabies Virus Strain Moscow 3253 Adapted to a Vero Cell Line

2020 ◽  
Vol 35 (4) ◽  
pp. 237-242
Author(s):  
Ya. M. Krasnov ◽  
Zh. V. Alkhova ◽  
S. V. Generalov ◽  
I. V. Tuchkov ◽  
E. A. Naryshkina ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Vero Cell ◽  
Whole Genome Sequencing ◽  
Cell Line ◽  
Rabies Virus ◽  
Genome Sequencing ◽  
Virus Strain ◽  
Whole Genome ◽  
Vero Cell Line ◽  
Rabies Virus Strain

scholarly journals Point Mutations in the Glycoprotein Ectodomain of Field Rabies Viruses Mediate Cell Culture Adaptation through Improved Virus Release in a Host Cell Dependent and Independent Manner

Viruses ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1989
Author(s):  
Sabine Nitschel ◽  
Luca M. Zaeck ◽  
Madlin Potratz ◽  
Tobias Nolden ◽  
Verena te Kamp ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Lines ◽  
Rabies Virus ◽  
Point Mutations ◽  
Inverse Correlation ◽  
Virus Release ◽  
Independent Manner ◽  
Adaptive Mutations ◽  
Field Virus

Molecular details of field rabies virus (RABV) adaptation to cell culture replication are insufficiently understood. A better understanding of adaptation may not only reveal requirements for efficient RABV replication in cell lines, but may also provide novel insights into RABV biology and adaptation-related loss of virulence and pathogenicity. Using two recombinant field rabies virus clones (rRABV Dog and rRABV Fox), we performed virus passages in three different cell lines to identify cell culture adaptive mutations. Ten passages were sufficient for the acquisition of adaptive mutations in the glycoprotein G and in the C-terminus of phosphoprotein P. Apart from the insertion of a glycosylation sequon via the mutation D247N in either virus, both acquired additional and cell line-specific mutations after passages on BHK (K425N) and MDCK-II (R346S or R350G) cells. As determined by virus replication kinetics, complementation, and immunofluorescence analysis, the major bottleneck in cell culture replication was the intracellular accumulation of field virus G protein, which was overcome after the acquisition of the adaptive mutations. Our data indicate that limited release of extracellular infectious virus at the plasma membrane is a defined characteristic of highly virulent field rabies viruses and we hypothesize that the observed suboptimal release of infectious virions is due to the inverse correlation of virus release and virulence in vivo.

scholarly journals CELL CULTURE AS THE MOST CERTAIN WAY OF DIAGNOSIS IN RABIES INFECTION

2020 ◽  
Vol 3 (1) ◽  
pp. 21
Author(s):  
Ariyani Noviantari ◽  
Khariri Khariri
Keyword(s):  
Cell Culture ◽  
Cell Lines ◽  
Rabies Virus ◽  
Neuroblastoma Cells ◽  
Cell Types ◽  
Culture Cell ◽  
Virus Antigen ◽  
Hek 293 ◽  
Brain And Spinal Cord ◽  
The Brain

Rabies is a zoonotic disease that causes progressive and fatal inflammation of the brain and spinal cord. This disease is still widespread in the world and is a social and economic burden in many countries. Rabies is estimated to cause 60.000 human deaths annually in over 150 countries, with 95% of cases occurring in Africa and Asia. Several countries in Southeast Asia, including Indonesia, have become one of the endemic areas for rabies. The diagnosis of rabies infection in animals and humans can be made by histopathology, virus cultivation, serology, and virus antigen detection This paper discusses cell culture as a method in the diagnosis of rabies. This paper is a literature review through a literature search in scientific journals and research reports that explains the various studies on the isolation of Rabies virus in cell culture, cell types used, and the technique in diagnosing Rabies infection in cell culture. The diagnosis of rabies can be made by neuroblastoma cells, BHK-21 cell lines, HEK-293 cell lines, and others. It can be concluded that the diagnosis of Rabies virus can be done on several cell lines and needs to be followed by other tests. The nature of rabies disease dictates that laboratory tests be standardized, rapid, sensitive, specific, economical, and reliable.

scholarly journals Use of Cell Culture-PCR Assay Based on Combination of A549 and BGMK Cell Lines and Molecular Identification as a Tool To Monitor Infectious Adenoviruses and Enteroviruses in River Water

2004 ◽  
Vol 70 (11) ◽  
pp. 6695-6705 ◽  
Author(s):  
Cheonghoon Lee ◽  
Seung-Hoon Lee ◽  
Euiri Han ◽  
Sang-Jong Kim
Keyword(s):  
Cell Culture ◽  
Phylogenetic Analysis ◽  
River Water ◽  
Cell Lines ◽  
Molecular Identification ◽  
A549 Cells ◽  
Enteric Viruses ◽  
Pcr Assay ◽  
Group 2 ◽  
Group 1

ABSTRACT Viral contamination in environmental samples can be underestimated because a single cell line might reproduce only some enteric viruses and some enteric viruses do not exhibit apparent cytopathic effects in cell culture. To overcome this problem, we evaluated a cell culture-PCR assay based on a combination of A549 and Buffalo green monkey kidney (BGMK) cell lines as a tool to monitor infectious adenoviruses and enteroviruses in river water. Water samples were collected 10 times at each of four rivers located in Gyeonggi Province, South Korea, and then cultured on group 1 cells (BGMK cells alone) and group 2 cells (BGMK and A549 cells). Reverse transcription and multiplex PCR were performed, followed by phylogenetic analysis of the amplicons. Thirty (75.0%) of the 40 samples were positive for viruses based on cell culture, and the frequency of positive samples grown on group 2 cells (65.0%) was higher than the frequency of positive samples grown on group 1 cells (50.0%). The number of samples positive for adenoviruses was higher with A549 cells (13 samples) than with BGMK cells (one sample); the numbers of samples positive for enteroviruses were similar with both types of cells. By using phylogenetic analysis, adenoviral amplicons were grouped into subgenera A, C, D, and F, and enteroviral amplicons were grouped into coxsackieviruses B3 and B4 and echoviruses 6, 7, and 30, indicating that A549 and BGMK cells were suitable for recovering a wide range of adenoviral and enteroviral types. The cell culture-PCR assay with a combination of A549 and BGMK cells and molecular identification could be a useful tool for monitoring infectious adenoviruses and enteroviruses in aquatic environments.

Comparative study of rabies virus persistence in human and hamster cell lines.

1981 ◽  
Vol 37 (1) ◽  
pp. 1-6 ◽  
Author(s):  
O G Andzhaparidze ◽  
N N Bogomolova ◽  
Y S Boriskin ◽  
M S Bektemirova ◽  
I D Drynov
Keyword(s):  
Comparative Study ◽  
Cell Lines ◽  
Rabies Virus ◽  
Virus Persistence

scholarly journals PC12 and THP-1 Cell Lines as Neuronal and Microglia Model in Neurobiological Research

2021 ◽  
Vol 11 (9) ◽  
pp. 3729
Author(s):  
Katarzyna Balon ◽  
Benita Wiatrak
Keyword(s):  
Cell Culture ◽  
Dna Damage ◽  
Cell Lines ◽  
Inflammatory Factor ◽  
Research Model ◽  
Differentiated Cells ◽  
Undifferentiated Cells ◽  
Primary Cell Lines ◽  
Neurobiological Research ◽  
The Impact

Models based on cell cultures have become a useful tool in modern scientific research. Since primary cell lines are difficult to obtain and handle, neoplasm-derived lines like PC12 and THP-1 offer a cheap and flexible solution for neurobiological studies but require prior differentiation to serve as a neuronal or microglia model. PC12 cells constitute a suitable research model only after differentiation by incubation with nerve growth factor (NGF) and THP-1 cells after administering a differentiation factor such as phorbol 12-myristate-13-acetate (PMA). Still, quite often, studies are performed on these cancer cells without differentiation. The study aimed to assess the impact of PC12 or THP-1 cell differentiation on sensitivity to harmful factors such as Aβ25-35 (0.001–5 µM) (considered as one of the major detrimental factors in the pathophysiology of Alzheimer’s disease) or lipopolysaccharide (1–100 µM) (LPS; a pro-inflammatory factor of bacterial origin). Results showed that in most of the tests performed, the response of PC12 and THP-1 cells induced to differentiation varied significantly from the effect in undifferentiated cells. In general, differentiated cells showed greater sensitivity to harmful factors in terms of metabolic activity and DNA damage, while in the case of the free radicals, the results were heterogeneous. Obtained data emphasize the importance of proper differentiation of cell lines of neoplastic origin in neurobiological research and standardization of cell culture handling protocols to ensure reliable results.

Sign in / Sign up

Export Citation Format

Share Document