Effect of different concentrations of Aloe vera leave\'s extract on the healing process of rat\'s second degree burn

Abstract Introduction Burn is one of the most severe traumas that causes coagulative destruction of the skin. The use of various products that accelerate wound healing in patients with burn may affect the patient’s survival and reduce the complications that may be seen. In the present study we aimed effects of subcutaneous ozone injection on second degree burn wound. Methods A total of 72 Sprague-Dawley male rats included in the study were divided randomly into three groups (control group (CG), silver sulfadiazine group (SG), ozone group (OG)) and each group was divided randomly two subgroups (as sacrificed on d7 and on d14).A deep second degree scald burns were created on the lower back. In CG subcutaneous 0.9% serum saline was injected daily into the burn area. In SG, burns were dressed with silver sulfadiazine daily and in OG subcutaneous ozone was injected daily into the burn area. Tissue hydroxyproline level measurement and histopathological evaluation were done. Results When the groups were compared in terms of weight change, no significant difference was found on the 7th and 14th days. In the evaluation made in terms of tissue hydroxyproline, tissue hydroxyproline level in OG was found to be significantly higher on both the 7th and 14th days (p < 0.001). In histopathological evaluations, it was determined that wound healing in OG was significantly higher than in the other groups. Conclusions According to the results, subcutaneous ozone therapy is more effective than silver sulphadiazine in the healing process of second-degree burn wounds and it can be safely used in the treatment of burn wounds.

Download Full-text

Effect of green tea on the second degree burn wounds in rats

Indian Journal of Plastic Surgery ◽

10.4103/0970-0358.146593 ◽

2014 ◽

Vol 47 (03) ◽

pp. 370-374 ◽

Cited By ~ 8

Author(s):

Mohammad Javad Fatemi ◽

Babak Nikoomaram ◽

Amir Asadollah Khajeh Rahimi ◽

Donya Talayi ◽

Shahrzad Taghavi ◽

...

Keyword(s):

Green Tea ◽

Healing Process ◽

Digital Camera ◽

First Aid ◽

Control Group ◽

Burn Wounds ◽

Beneficial Effects ◽

Pathologic Evaluation ◽

Degree Burn ◽

Second Degree

ABSTRACT Background: Various studies indicate that the green tea has anti-inflammatory and anti-oxidative properties. Moreover, a few studies have been carried out that demonstrate beneficial effects of green tea on burned patients. Materials and Methods: In this study, green tea, Vaseline, and silver sulfadiazine dressings were used as first-aid treatment to deep dermal contact burns in rats, compared with a control of nothing. After creating second-degree burn on the dorsum of rats, the treatments were applied for 15 min in four groups. Wound dressing changes were daily. Macroscopic study was performed on days 1, 3, 7, and 14 by using a digital camera and software processing of photos. Microscopic examination was done by pathologic evaluation of skin specimens on day 14. Results: We observed that green tea usage significantly decreased burn size in comparison to the control group (P = 0.004). Conclusion: Green tea is effective on healing process of second degree burn wounds.

Download Full-text

ALOE VERA VS. SILVER SULFADIAZINE FOR TREATING SECOND-DEGREE BURN WOUNDS: EVIDENCE-BASED CASE REPORT

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2019.v11s6.33580 ◽

2019 ◽

pp. 146-148

Author(s):

CLARA MENNA ◽

NABILLA CALISTA ◽

LEORCA AURINO ◽

ADISTI DWIJAYANTI

Keyword(s):

Clinical Trials ◽

Risk Reduction ◽

Randomized Clinical Trials ◽

Aloe Vera ◽

Silver Sulfadiazine ◽

Number Needed To Treat ◽

Burn Wounds ◽

The Third ◽

Degree Burn ◽

Second Degree

Objective: Current treatment for second-degree burn wounds, including silver sulfadiazine (SSD) application, may cause side effects such as delayed and incomplete wound healing, leaving a scar. Traditional plants empirically used for burn wounds, such as Aloe vera, seem to be a promising option with good safety profiles. Methods: We therefore compared Aloe vera to SSD for second-degree burn wounds based on a review of clinical trials obtained by an organized search through the Cochrane, PubMed, and DynaMed databases. Three randomized clinical trials studies were selected, two of which were critically appraised. The third study, with a different outcome assessment, was analyzed separately. Results: Two studies showed that Aloe vera was more effective in treating second-degree burn wounds than SSD 1% with an absolute risk reduction of 0.2, relative risk reduction of 0.83–1.0, and number needed to treat five patients. The Aloe vera group showed faster healing, earlier epithelialization, and earlier pain alleviation. The third study combined Aloe vera with other herbs, which showed better healing ability and restored skin hydration than those with SSD. Conclusion: Each of the three articles stated that Aloe vera was more effective than SSD for treating second-degree burn wounds. Nevertheless, each of the studies lacked methodology and comprehensive data reporting.

Download Full-text

Effect of mesenchymal stem cells versus aloe vera on healing of deep second-degree burn

Stem Cell Investigation ◽

10.21037/sci-2020-030 ◽

2021 ◽

Vol 8 ◽

pp. 12-12

Author(s):

Nahla Imbarak ◽

H. Ismail Abdel-Aziz ◽

Lamiaa M. Farghaly ◽

Somaya Hosny

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Aloe Vera ◽

Degree Burn ◽

Second Degree

Download Full-text

NaHS-Hydrogel and Encapsulated Adipose-Derived Stem Cell Evaluation on an Ex Vivo Second-Degree Burn Model

European Journal of Burn Care ◽

10.3390/ebj2010002 ◽

2021 ◽

Vol 2 (1) ◽

pp. 9-30

Author(s):

Lucille Capin ◽

Olivia Gross-Amat ◽

Marie Calteau ◽

Marie-Rose Rovere ◽

Damien Salmon ◽

...

Keyword(s):

Wound Healing ◽

Ex Vivo ◽

Healing Process ◽

Intradermal Injection ◽

Proliferating Cells ◽

Burn Treatment ◽

Degree Burn ◽

Collagen Iii ◽

Procollagen Iii ◽

Second Degree

Second-degree burns result in the loss of the epidermal barrier and could lead to delayed complications during the healing process. Currently, therapeutic options to treat severe burns are limited. Thus, this work aims to evaluate the effect of NaHS, a hydrogen sulfide (H2S) donor, in poloxamer hydrogel in topical application and the potentiating effect of injected encapsulated adipose-derived stem cells (ASCs) compared to monolayer ASCs using our previous second-degree burn model on human skin explants. Indeed, our model allows testing treatments in conditions similar to a clinical application. The observed benefits of NaHS may include an antioxidant role, which might be beneficial in the case of burns. Concerning ASCs, their interest in wound healing is more than well documented. In order to evaluate the efficiency of our treatments, we analyzed the kinetics of wound closure, keratinocyte proliferation, and dermal remodeling. The effect of NaHS led to a delay in re-epithelialization, with a decrease in the number of proliferating cells and a decrease in the synthesis of procollagen III. On the contrary, intradermal injection of ASCs, encapsulated or not, improves wound healing by accelerating re-epithelialization and collagen I synthesis; however, only encapsulated ASCs accelerate keratinocyte migration and increase the rate of procollagen III and collagen III. In conclusion, NaHS treatment did not improve burn healing. However, the injection of ASCs stimulated wound healing, which is encouraging for their therapeutical use in burn treatment.

Download Full-text

Burn Ointment Promoted Cutaneous Wound for Burns and Scalds by Modulating the PI3K/AKT/mTOR Signaling Pathway

10.21203/rs.3.rs-42754/v1 ◽

2020 ◽

Author(s):

Da li Gan ◽

Yan Yao ◽

Qi yuan Su ◽

Su qin Yang ◽

Li Wu ◽

...

Keyword(s):

Wound Healing ◽

Healing Process ◽

Skin Irritation ◽

Mtor Pathway ◽

Epithelial Tissue ◽

Burn Wounds ◽

Degree Burn ◽

Tnf Α ◽

Tgf Β1 ◽

Second Degree

Abstract Background: Burn injury is common, burn ointment (BO) is a common preparation used to treat burns and scalds in folk as an effective remedy for burn healing. The present study was undertaken to evaluate the healing effect and related underlying mechanisms of BO in a model of deep second-degree thermal burn by animal experiments. Methods: BO is was made up of a combination of extracts from several traditional Chinese medicine and borneol, solid paraffin, rapeseed oil, and and its quality control was assessed. The acute toxicity test and skin irritation test were evaluated by rats. The anti-inflammatory effect was revealed by using inflammation animal models, including the xylene-induced auricle swelling in mice and carrageenan-induced toe swelling in rats. The hot plate test was used to evaluate its analgesic activity. Moreover, the experiments of knife and a deep second-degree burn wound were used to explore the effect of BO in promoting wound healing. On days 7, 14 and 28, the wounds were digitally photographed by a camera and after sacrifice of the SD rats, skin samples were obtained for performing H&E staining, immunohistochemical staining and Western Blotting examination. In addition, The expressed of TNF-α, TGF-β1 and VEGF in serum were detected by ELISA kits. Results: BO had no toxicity or side effects on the skin and liver or kidney function. BO could significantly inhibit auricular swelling in mice, paw welling in rats and markedly prolonged the latencies of licking paws in mice; it also could accelerate the process of wound healing and repair scar by promoting the formation of new epithelial tissue. In addition, BO significantly reduced the content of TNF-α and markedly increased the content of VEGF and TGF-β1 in the serum. Moreover, BO promoted the expression of collagen I. Furthermore, it increased the ratio of p-PI3K/PI3K, p-AKT/AKT and p-mTOR/mTOR in the PI3K / AKT / mTOR pathway.Conclusions: BO could effectively reduce inflammation and pain, and effectively accelerate the healing process of deep second-degree burn wounds. And the mechanism of BO promoting wound healing may be related to activate PI3K / AKT / mTOR pathway. therefore, it may be recommended as a promising topical medication for treating burn wounds in the future clinical trials.

Download Full-text

Effect of Subcutaneous Topical Ozone Therapy on Second-Degree Burn Wounds in Rats: An Experimental Study

Journal of Burn Care & Research ◽

10.1093/jbcr/irab110 ◽

2021 ◽

Author(s):

Emre Karakaya ◽

Aydincan Akdur ◽

Ebru Ayvazoğlu Soy ◽

Coşkun Araz ◽

Alev Ok Atilgan ◽

...

Keyword(s):

Wound Healing ◽

Healing Process ◽

Silver Sulfadiazine ◽

Male Rats ◽

Control Group ◽

Ozone Therapy ◽

Burn Wounds ◽

Degree Burn ◽

Significant Difference ◽

Second Degree

Abstract Burns are one of the most severe traumas, causing coagulative destruction of the skin. The use of various products that accelerate wound healing in patients with burns may affect rates of patient survival and reduce complications. We studied the effects of subcutaneous ozone injection on second-degree burn wounds in animal model. For this study, 72 Sprague-Dawley male rats were divided randomly into the following three groups: control group , silver sulfadiazine group, and ozone group; each group was then divided randomly into two subgroups (day 7 or day 14 examination and sacrifice). Superficial partial thickness burns were created on the lower back. In the control group, subcutaneous 0.9% serum saline was injected daily into the burn area. In the silver sulfadiazine group, burns were dressed daily with silver sulfadiazine. In the ozone group, subcutaneous ozone was injected daily into the burn area. We performed tissue hydroxyproline level measurements and histopathological evaluations. When groups were compared in terms of weight change, no significant difference was found between day 7 and day 14. With regard to tissue hydroxyproline levels the ozone group had significantly higher levels on both day 7 and day 14 (P < .001). In histopathological evaluations, we determined that wound healing in the ozone group was significantly higher than in the other groups. We found that subcutaneous ozone therapy was more effective than silver sulfadiazine in the healing process of second-degree burn wounds and could be safely used in the treatment of burn wounds.

Download Full-text

Effect of Honey, Aloe vera, and MEBO on Collagen Density in Healing Process of Second Degree Burns in Rats

AL-Kauniyah Jurnal Biologi ◽

10.15408/kauniyah.v14i1.16734 ◽

2021 ◽

Vol 14 (1) ◽

pp. 152-161

Author(s):

Ovelia Yolanda ◽

Sekar Dorojati Yuliana ◽

Yudhi Nugraha

Keyword(s):

Wound Healing ◽

Topical Application ◽

Healing Process ◽

Aloe Vera ◽

Collagen Formation ◽

Bonferroni Test ◽

Collagen Density ◽

Post Hoc ◽

One Way Anova ◽

Second Degree

AbstrakBerbagai penelitian ilmiah telah membuktikan bahwa kolagen merupakan biomaterial ideal dalam aktivitas penyembuhan luka. Senyawa bioaktif pada madu dan Aloe vera dapat membantu meningkatkan pembentukan kolagen. Penelitian ini dilakukan untuk membandingkan pengaruh aplikasi topikal madu, gel Aloe vera, dan Moist Exposed Burn Ointment (MEBO) terhadap kepadatan kolagen pada proses penyembuhan luka bakar derajat dua pada tikus. Luka bakar dibuat pada 28 ekor tikus dan dibagi menjadi empat kelompok yang dipilih secara acak serta dilakukan aplikasi topikal harian dengan NaCl, madu, gel Aloe vera, dan MEBO secara berurutan. Biopsi kulit dilakukan pada hari ke-7, kemudian dilakukan pembuatan sediaan histopatologi kulit dan dihitung kepadatan kolagennya. Uji One Way ANOVA menunjukkan kepadatan kolagen berbeda secara signifikan antar masing-masing kelompok (p= 0,009). Uji Post Hoc Bonferroni menunjukkan terdapat perbedaan yang signifikan antara kelompok NaCl dengan madu (p= 0,024) dan NaCl dengan MEBO (p= 0,024). Penelitian ini menunjukkan bahwa aplikasi madu dan MEBO secara topikal pada luka bakar derajat dua dapat meningkatkan pembentukan kolagen, sehingga dapat mempercepat proses penyembuhan luka. Madu, Aloe vera, dan MEBO dapat dijadikan sebagai terapi alternatif dalam penyembuhan luka bakar. AbstractVarious scientific studies have proven that collagen is an ideal biomaterial in wound healing activities. The bioactive compounds in honey and Aloe vera can help increase collagen formation. This study was conducted to compare the effect of topical application of honey, Aloe vera gel, and MEBO (Moist Exposed Burn Ointment) on collagen density in the healing process of second-degree burns in rats. Burns were made on 28 rats which were further randomly divided into four groups to receive daily topical application of NaCl, Aloe vera, honey, and MEBO respectively. Skin biopsy was carried out on the seventh day, then histopathological preparation of the skin was made and collagen density was calculated. Result of One Way ANOVA test showed that collagen density differed significantly between groups (p= 0.009). The Post Hoc Bonferroni test resulted in significant difference between NaCl with honey group (p= 0.024) and NaCl with MEBO (p= 0.024). This study found that topical application of honey and MEBO to second-degree burns could increase collagen formation, thus accelerating wound healing process. Honey, Aloe vera, and MEBO can be used as alternative therapies for healing burns.

Download Full-text