scholarly journals Comparison of Hematological and Biochemical Profiles of Late Pregnant and Non-Pregnant Mares

2021 ◽  
Vol 22 (1) ◽  
pp. 1-7
Author(s):  
Murat Onur Yazlik ◽  
Ezgi Dikmeoglu ◽  
Arzu Esen ◽  
Ufuk Kaya ◽  
Özgenur Kafkas ◽  
...  
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Biochemical Parameters ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Clinical Practices ◽  
Blood Cell Count ◽  
Corpuscular Hemoglobin ◽  
Mean Cell Volume ◽  
Pregnancy Status

The maternal body must adapt to fetal growth and development during the state of pregnancy for successful foal and mother. Pregnancy status as in other mammals is related to physiology and hematology. This study aimed to compare the hematology profiles and biochemical parameters of late pregnant and non-pregnant mares. Twenty-two mares were included in the study. Mares were divided into two groups according to the pregnancy status. Hematology profile and biochemical parameters were analyzed from blood samples collected from the jugular vein. All data were analyzed using Stata 12/MP4. White blood cell count, lymphocyte, red blood cell count, hemoglobin concentration, hematocrit, monocyte and percentage of monocyte were significantly lower in non-pregnant mares compared to late pregnant mares (P<0.05). No significant differences were noted in values of neutrophil, eosinophil, mean cell volume, mean corpuscular hemoglobin concentration, mean corpuscular hemoglobin, platelet, mean platelet volume, red cell distribution width in both groups (P>0.05). On the other hand, biochemical parameters analysis showed that calcium, total bilirubin, and triglyceride concentrations were greater in late pregnant mares (P<0.05). However, urea, albumin, cholesterol, and magnesium concentrations were similar among groups (P>0.05). In conclusion, the present study showed hematology profile and biochemical parameters differences in late pregnant and non-pregnant mares. These values could be useful in clinical practices to assess the situation of late pregnant mares.

scholarly journals Hematological parameters in Nile Tilápia, Oreochromis niloticus exposed to sub-letal concentrations of mercury

2007 ◽  
Vol 50 (4) ◽  
pp. 619-626 ◽  
Author(s):  
Nilton Massuo Ishikawa ◽  
Maria José Tavares Ranzani-Paiva ◽  
Julio Vicente Lombardi ◽  
Cláudia Maris Ferreira
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Oreochromis Niloticus ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Control Group ◽  
Mean Corpuscular Hemoglobin ◽  
Blood Cell Count ◽  
Corpuscular Hemoglobin ◽  
Set Up

Mercury toxicity in tilapia, Oreochromis niloticus, (Linnaeus, 1758) was investigated by the hematological parameters after long-term (14 days) exposure to various Hg concentrations (0.02, 0.002, 0.0002mg/L Hg). Test groups were set up with three replicates for each concentration, plus the control group. Blood samples were collected from six individuals for each concentration at 0, 3, 7, 10 and 14 days of exposure. The hematological parameters analyzed were: total red blood cell count (RBC), hemoglobin concentration (Hb), hematocrit (Ht), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), total white blood cell count (WBC) and differential leukocyte counts and total thrombocyte count (Tr). There were no significant differences among the mean hematological values at the different Hg concentrations indicating that Hg at the concentrations studied was not toxic to tilapia.

scholarly journals Hematology values of captive giant anteaters (Myrmecophaga tridactyla) and collared anteaters (Tamandua tetradactyla)

2013 ◽  
Vol 33 (4) ◽  
pp. 557-560 ◽  
Author(s):  
Thaís C. Sanches ◽  
Flávia R. Miranda ◽  
Alice S. Oliveira ◽  
Eliana R. Matushima
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Hemoglobin Concentration ◽  
Relevant Information ◽  
Disease Diagnosis ◽  
Sao Paulo ◽  
Mean Corpuscular Hemoglobin ◽  
São Paulo ◽  
Blood Cell Count ◽  
Corpuscular Hemoglobin

Hematological results are reported for 13 giant anteaters (Myrmecophaga tridactyla) and 13 collared anteaters (Tamandua tetradactyla). Animals were captive-reared adults held at the Fundação Parque Zoológico de São Paulo (São Paulo, SP, Brazil) and Parque Zoológico Municipal Quinzinho de Barros (Sorocaba, SP, Brazil), and were considered healthy on physical examination. Examined parameters included red blood cell count, white blood cell count, hematocrit, hemoglobin concentration, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, total plasmatic protein and differential leukocyte counts. Also, a survey for hemoparasites was done and none was observed in thin blood smears. The results were generally similar to those previously reported in the exiguous literature for these species, providing further reference data for the interpretation of laboratory results besides health monitoring, assisting early disease diagnosis and providing relevant information for conservation programs for these species.

scholarly journals Development of Novel Continuous and Interval Exercise Programs by Applying the FITT-VP Principle in Dogs

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
H. S. Lee ◽  
S. H. Lee ◽  
J. W. Kim ◽  
Y. S. Lee ◽  
B. C. Lee ◽  
...  
Keyword(s):  
Exercise Training ◽  
Blood Cell ◽  
Cell Count ◽  
Mean Corpuscular Hemoglobin ◽  
Reference Ranges ◽  
Blood Cell Count ◽  
Corpuscular Hemoglobin ◽  
Exercise Programs ◽  
Interval Exercise ◽  
Essential Components

Although proper exercise training induces positive physiological effects, improper exercise can lead to injury, fatigue, and poor performance. The frequency, intensity, time/duration, type, volume, and progression (FITT-VP) are the essential components of exercise training to maintain or improve physical fitness and health. The purpose of this study was to develop specific exercise programs by applying the FITT-VP principle and to examine the effects on heart rate (HR) and hematological and biochemical parameters in dogs. The healthy male Beagles (n = 4) included in this study performed continuous and interval exercises, comprising 12 protocols. The HR monitoring elicited an affirmative response to activities but varied depending on the protocols. The hematologic parameters (e.g., red blood cell count, white blood cell count, hemoglobin, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration) were within the reference ranges both before and after exercise. The creatine kinase level significantly increased, and the cholesterol level decreased after exercises. In conclusion, the continuous and interval exercise program elicits an appropriate HR reaction, has no adverse effects on the serum parameters, and provides valuable insight for healthcare in dogs.

scholarly journals Hematological changes in Eleginops maclovinus during an experimental Caligus rogercresseyi infestation

2013 ◽  
Vol 22 (3) ◽  
pp. 402-406 ◽  
Author(s):  
Patricio Peña-Rehbein ◽  
Karin Ruiz ◽  
Alexander Ortloff ◽  
María Isabel Pizarro ◽  
Carolina Navarrete
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
White Blood Cell Count ◽  
White Blood Cell ◽  
Hemoglobin Level ◽  
Mean Corpuscular Hemoglobin ◽  
Reference Ranges ◽  
Blood Cell Count ◽  
Corpuscular Hemoglobin ◽  
Eleginops Maclovinus

Eleginops maclovinus has been an important fishery resource in Chile since 1957. Caligus rogercresseyi is one of the most prevalent ectoparasite species found on E. maclovinus. Hematocrit, hemoglobin level, red blood cell count (RBC), white blood cell count (WBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) and differential white blood cell count were determined before and after an experimental infestation withC. rogercresseyi. We found significant differences in the hemoglobin level, WBC, MCV, MCH, MCHC, hematocrit level and RBC between infested and uninfested fish. Furthermore correlations between number of C. rogercresseyi with hematocrit, MCHC, neutrophil, eosinophil and lymphocyte counts were found. Hematological reference ranges of E. maclovinus in captivity conditions were also established.

scholarly journals PSIV-27 Peripheral complete blood cell count results in pigs are impacted by gender, sow parity, and farrowing season

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 291-292
Author(s):  
Elle Rottman ◽  
Alisun N Watson ◽  
Catherine Buck ◽  
Tsungcheng Tsai ◽  
Jeffery J Chewning ◽  
...  
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Whole Blood ◽  
Blood Cells ◽  
White Blood Cells ◽  
Mean Corpuscular Hemoglobin ◽  
Complete Blood Cell Count ◽  
Blood Cell Count ◽  
Corpuscular Hemoglobin ◽  
Cell Counts

Abstract Complete blood cell counts have been used as a diagnostic tool across many animal species including swine. To investigate the factors that cause variation in complete blood cell count results, a total of 2,284 whole blood samples were collected from 2012 to 2019 in preweaning piglets (n = 518), nursery pigs (n = 1,704), and grower pigs (n = 60). Whole blood was collected into K2EDTA blood collection tubes and assayed using an automatic hematologic analyzer within 6 hours of collection. Data were analyzed by Mixed procedure of SAS with gender, parity group, and farrowing season as fixed effects. Body weight and age of pigs served as covariances. Farrowing season was grouped into summer (born during May to October) or winter (or November to April). Pigs that were born from first, second, and third parity, and four and above parity sows were assorted into parity group 1, 2 to 3, and 4+, respectively. Barrows had a greater concentration of total white blood cells (P &lt; 0.01), lymphocytes (P &lt; 0.01), and neutrophils (P &lt; 0.01) compared to gilts. Barrows had lower mean corpuscular volume (P = 0.03), mean corpuscular hemoglobin (P &lt; 0.01), and mean corpuscular hemoglobin concentration (P = 0.02) compared to gilts. Pigs that were farrowed in the winter season had a greater concentration of white blood cells (P = 0.01), neutrophils (P = 0.01), and the percentage of neutrophils (P = 0.03), but were lower in the percentage of lymphocytes (P = 0.03) compared to pigs farrowed during summer. Pigs born to parity four and above sows obtained a greater lymphocyte count (P = 0.01), percentage of neutrophils (P = 0.02), and percentage of lymphocytes (P = 0.01). We concluded that peripheral complete blood cells count results were affected by gender, farrowing season, and sow parity.

scholarly journals Complete Blood Count Peculiarities in Pregnant SARS-CoV-2-Infected Patients at Term: A Cohort Study

Diagnostics ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 80
Author(s):  
Roxana Covali ◽  
Demetra Socolov ◽  
Razvan Socolov ◽  
Ioana Pavaleanu ◽  
Alexandru Carauleanu ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Blood Count ◽  
Complete Blood Count ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Mean Corpuscular Hemoglobin Concentration ◽  
Blood Cell Count ◽  
Corpuscular Hemoglobin ◽  
Distribution Width

Background: During viral outbreaks, pregnancy poses an increased risk of infection for women. Methods: In a prospective study, all patients admitted for delivery at term to Elena Doamna Obstetrics and Gynecology University Hospital in Iasi, Romania, between 1 April 2020 and 31 December 2020 were included. There were 457 patients, divided into two groups: group 1, SARS-CoV-2-positive patients (n = 46) and group 2, SARS-CoV-2-negative patients (n = 411). Among other tests, complete blood count was determined upon admittance, and the following values were studied: white blood cell count, lymphocytes, neutrophils, red blood cell count, hemoglobin, mean corpuscular hemoglobin concentration, mean corpuscular hemoglobin, mean corpuscular volume, red blood cell distribution width, hematocrit, platelet count, mean platelet volume, platelet distribution width, plateletcrit, and platelet large cell ratio. Results: in pregnant SARS-CoV-2-infected patients at term, there was a significant decrease in white blood cell, neutrophil, and lymphocyte count, and an increase in mean corpuscular hemoglobin concentration, compared to healthy pregnant women at term, although all still within normal limits. None of the other components of the complete blood count or fetal outcomes studied was significantly influenced by SARS-CoV-2 infection in pregnant patients at term.

The Aachen Minipig: Phenotype, Genotype, Hematological and Biochemical Characterization, and Comparison to the Göttingen Minipig

2017 ◽  
Vol 58 (5-6) ◽  
pp. 193-203 ◽  
Author(s):  
Kerstin Pawlowsky ◽  
Lisa Ernst ◽  
Julia Steitz ◽  
Thaddäus Stopinski ◽  
Babette Kögel ◽  
...  
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Reference Values ◽  
Biomedical Research ◽  
Clinical Chemistry ◽  
Mean Corpuscular Hemoglobin ◽  
Blood Cell Count ◽  
Corpuscular Hemoglobin ◽  
Organ Weights ◽  
Göttingen Minipig

Background: The pig is one of the most frequently used large animal models for biomedical research, especially in the field of translational research and surgical models. While standard livestock breeds are used in short-term and acute studies, minipig breeds are the preferred breeds in long-term and chronic studies due to their limited growth and body weight. Objective: In consideration of the 3R principle (refinement, reduction, replacement) and the increasing demand, the aim of this study was to generate a new, robust, non-specific-pathogen-free minipig breed, the Aachen minipig. Methods: Phenotype, genotype, and hematological as well as clinical chemistry parameters were characterized, and reference values of the Aachen minipig were generated and compared to the values in the commonly used Göttingen minipig. Organ weights of the heart, kidney, liver, lung, spleen, and brain were determined using a laboratory balance. Blood samples were collected for hematology and clinical chemistry. Assessment of genetic diversity was performed by microsatellite markers. Nasal swabs were collected from 11 individual minipigs representing 6 races for DNA extraction. DNA was quantified and the identity and origin of the Aachen minipigs at the genomic level was determined by microsatellites. Results: The Aachen minipig established here is based on the Mini-LEWE breed and consists of the Vietnamese potbelly pig, the Schwäbisch Hällisch Landpig, the German Landrace, and the Minnesota minipig. Relative organ weights (lung, heart, kidneys, brain), hematology (hemoglobin, hematocrit, platelet count, mean corpuscular hemoglobin concentration, segmented neutrophils, lymphocytes, eosinophils, basophils), and clinical chemistry parameters (sodium, calcium, chloride, alkaline phosphatase, aspartate aminotransferase, alanine aminotransferase, gamma-glutamyl transferase, lactate dehydrogenase, triglycerides, blood urea nitrogen, creatinine, total bilirubin, total protein, creatine kinase) of the Aachen minipigs and the Göttingen minipigs were not significantly different. Significant differences where only seen in relative organ weights (liver, spleen), hematology (red blood cell count, mean corpuscular volume, mean corpuscular hemoglobin, white blood cell count, banded neutrophils, monocytes), and clinical chemistry parameters (inorganic phosphorus, potassium, glucose, cholesterol, albumin, amylase). Conclusion: The Aachen minipig is a suitable model for research due to its similarity to other minipig breeds, especially the Göttingen minipig. The reference values established in this study may be used for the comparison of scientific data and encourage the use of the Aachen minipig as an animal model for biomedical research.

scholarly journals Evaluation of the automated hematology analyzer Sysmex XT-2000iV™ compared to the ADVIA® 2120 for its use in dogs, cats, and horses

2011 ◽  
Vol 23 (6) ◽  
pp. 1168-1180 ◽  
Author(s):  
Natali Bauer ◽  
Julia Nakagawa ◽  
Cathrin Dunker ◽  
Klaus Failing ◽  
Andreas Moritz
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Rank Correlation ◽  
Correlation Coefficients ◽  
Reference Method ◽  
Hemoglobin Concentration ◽  
Differential Count ◽  
Mean Corpuscular Hemoglobin ◽  
Blood Cell Count ◽  
Hematology Analyzer

The automated laser-based hematology analyzer Sysmex XT-2000 iV™ providing a complete blood cell count (CBC) and 5-part differential has been introduced in large veterinary laboratories. The aim of the current study was to determine precision, linearity, and accuracy of the Sysmex analyzer. Reference method for the accuracy study was the laser-based hematology analyzer ADVIA® 2120. For evaluation of accuracy, consecutive fresh blood samples from healthy and diseased cats ( n = 216), dogs ( n = 314), and horses ( n = 174) were included. A low intra-assay coefficient of variation (CV) of approximately 1% was seen for the CBC except platelet count (PLT). An intra-assay CV ranging between 2% and 5.5% was evident for the differential count except for feline and equine monocytes (7.7%) and horse eosinophils (15.7%). Linearity was excellent for white blood cell count (WBC), hematocrit value, red blood cell count (RBC), and PLT. For all evaluated species, agreement was excellent for WBC and RBC, with Spearman rank correlation coefficients (rs) ranging from >0.99 to 0.98. Hematocrit value correlated excellently in cats and dogs, whereas for horses, a good correlation was evident. A good correlation between both analyzers was seen in feline and equine PLT (rs = 0.89 and 0.92, respectively), whereas correlation was excellent for dogs (rs = 0.93). Biases were close to 0 except for mean corpuscular hemoglobin concentration (4.11 to −7.25 mmol/l) and canine PLT (57 × 109/l). Overall, the performance of the Sysmex analyzer was excellent and compared favorably with the ADVIA analyzer.

scholarly journals Blood parameter analysis and morphological alterations as biomarkers on the health of Hoplias malabaricus and Geophagus brasiliensis

2006 ◽  
Vol 49 (3) ◽  
pp. 441-448 ◽  
Author(s):  
Silvia Romão ◽  
Lucélia Donatti ◽  
Matheus O. Freitas ◽  
Josiane Teixeira ◽  
Josiana Kusma
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Hemoglobin Concentration ◽  
Parameter Analysis ◽  
Mean Corpuscular Hemoglobin ◽  
Blood Parameter ◽  
Blood Cell Count ◽  
Hoplias Malabaricus ◽  
Morphological Alterations ◽  
Geophagus Brasiliensis

This study aimed to assess the influence of the environment on fish health. Samples of Hoplias malabaricus and Geophagus brasiliensis, were collected from three different environments: area I was urban and areas II and III were rural. Analyses of red blood cell count, microhematocrit, hemoglobin concentration, white blood cell count and differential white cell count in blood smear were carried out. Mean corpuscular volume and mean corpuscular hemoglobin concentration were calculated. To analyze morphological alterations, gills, liver, kidney and gonads were submitted to routine histological processing. Individuals collected from area III had slightly lower blood indices than collected from area I . Severe kidney changes, degeneration of and crystallization within kidney tubules were observed. In area I, crystallization was observed in 92% of the specimens of G. brasiliensis. These results suggested that such alterations were related with poor water circulation in the place.

scholarly journals Complex Biological Profile of Hematologic Markers across Pediatric, Adult, and Geriatric Ages: Establishment of Robust Pediatric and Adult Reference Intervals on the Basis of the Canadian Health Measures Survey

2015 ◽  
Vol 61 (8) ◽  
pp. 1075-1086 ◽  
Author(s):  
Khosrow Adeli ◽  
Joshua E Raizman ◽  
Yunqi Chen ◽  
Victoria Higgins ◽  
Michelle Nieuwesteeg ◽  
...  
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Reference Intervals ◽  
Mean Corpuscular Hemoglobin ◽  
Blood Cell Count ◽  
Corpuscular Hemoglobin ◽  
Health Measures ◽  
Canadian Health ◽  
Canadian Health Measures Survey ◽  
Age And Sex

AbstractBACKGROUNDIn a collaboration between the Canadian Laboratory Initiative on Pediatric Reference Intervals (CALIPER) and the Canadian Health Measures Survey (CHMS), we determined reference value distributions using an a priori approach and created a comprehensive database of age- and sex-stratified reference intervals for clinically relevant hematologic parameters in a large household population of children and adults.METHODSThe CHMS collected data and blood samples from 11 999 respondents aged 3–79 years. Hematology markers were measured with either the Beckman Coulter HmX or Siemens Sysmex CA-500 Series analyzers. After applying exclusion criteria and removing outliers, we determined statistically relevant age and sex partitions and calculated reference intervals, including 90% CIs, according to CSLI C28-A3 guidelines.RESULTSHematology marker values showed dynamic changes from childhood into adulthood as well as between sexes, necessitating distinct partitions throughout life. Most age partitions were necessary during childhood, reflecting the hematologic changes that occur during growth and development. Hemoglobin, red blood cell count, hematocrit, and indices (mean corpuscular volume, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration) increased with age, but females had lower hemoglobin and hematocrit starting at puberty. Platelet count gradually decreased with age and required multiple sex partitions during adolescence and adulthood. White blood cell count remained relatively constant over life, whereas fibrinogen increased slightly, requiring distinct age and sex partitions.CONCLUSIONSThe robust dataset generated in this study has allowed observation of dynamic biological profiles of several hematology markers and the establishment of comprehensive age- and sex-specific reference intervals that may contribute to accurate monitoring of pediatric, adult, and geriatric patients.

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