scholarly journals Evaluation of the automated hematology analyzer Sysmex XT-2000iV™ compared to the ADVIA® 2120 for its use in dogs, cats, and horses

2011 ◽  
Vol 23 (6) ◽  
pp. 1168-1180 ◽  
Author(s):  
Natali Bauer ◽  
Julia Nakagawa ◽  
Cathrin Dunker ◽  
Klaus Failing ◽  
Andreas Moritz
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Rank Correlation ◽  
Correlation Coefficients ◽  
Reference Method ◽  
Hemoglobin Concentration ◽  
Differential Count ◽  
Mean Corpuscular Hemoglobin ◽  
Blood Cell Count ◽  
Hematology Analyzer

The automated laser-based hematology analyzer Sysmex XT-2000 iV™ providing a complete blood cell count (CBC) and 5-part differential has been introduced in large veterinary laboratories. The aim of the current study was to determine precision, linearity, and accuracy of the Sysmex analyzer. Reference method for the accuracy study was the laser-based hematology analyzer ADVIA® 2120. For evaluation of accuracy, consecutive fresh blood samples from healthy and diseased cats ( n = 216), dogs ( n = 314), and horses ( n = 174) were included. A low intra-assay coefficient of variation (CV) of approximately 1% was seen for the CBC except platelet count (PLT). An intra-assay CV ranging between 2% and 5.5% was evident for the differential count except for feline and equine monocytes (7.7%) and horse eosinophils (15.7%). Linearity was excellent for white blood cell count (WBC), hematocrit value, red blood cell count (RBC), and PLT. For all evaluated species, agreement was excellent for WBC and RBC, with Spearman rank correlation coefficients (rs) ranging from >0.99 to 0.98. Hematocrit value correlated excellently in cats and dogs, whereas for horses, a good correlation was evident. A good correlation between both analyzers was seen in feline and equine PLT (rs = 0.89 and 0.92, respectively), whereas correlation was excellent for dogs (rs = 0.93). Biases were close to 0 except for mean corpuscular hemoglobin concentration (4.11 to −7.25 mmol/l) and canine PLT (57 × 109/l). Overall, the performance of the Sysmex analyzer was excellent and compared favorably with the ADVIA analyzer.

scholarly journals Evaluation of the automated hematology analyzer Sysmex XT-2000iV™ compared to the ADVIA® 2120 for its use in dogs, cats, and horses. Part II

2012 ◽  
Vol 24 (1) ◽  
pp. 74-89 ◽  
Author(s):  
Natali Bauer ◽  
Julia Nakagawa ◽  
Cathrin Dunker ◽  
Klaus Failing ◽  
Andreas Moritz
Keyword(s):  
Rank Correlation ◽  
Correlation Coefficients ◽  
Differential Count ◽  
Mean Corpuscular Hemoglobin ◽  
Cell Counts ◽  
Hematology Analyzer ◽  
Automated Hematology Analyzer ◽  
The Impact ◽  
And Storage ◽  
Edta Blood

The automated laser-based hematology analyzer Sysmex XT-2000 iV™ provides a 5-part differential count and specific cytograms that are of great interest for large veterinary laboratories. The aim of the study was to validate the Sysmex XT-2000 iV compared to the laser-based hematology analyzer ADVIA® 2120 and manual differential in dogs, cats, and horses as well as the impact of anticoagulant (heparin, ethylenediamine tetra-acetic acid [EDTA], and citrate) and storage at 22°C and 4°C. Consecutive fresh K3–EDTA blood samples from 216 cats, 314 dogs, and 174 horses were included. The impact of anticoagulant and sample storage was assessed in specimens obtained from an additional 9 cats, 10 dogs, and 10 horses. Agreement between both analyzers was excellent to good except for monocytes and canine reticulocytes. Spearman rank correlation coefficients ( rs) between Sysmex XT-2000 iV and manual differential were good to fair and ranged from 0.91 (cat lymphocytes) to 0.44 (cat monocytes). Hematocrit value (Hct), mean corpuscular hemoglobin (MCH), MCH concentration (MCHC; all: P < 0.001), and mean corpuscular volume (MCV; P < 0.01) were higher in canine citrated blood compared to heparin and EDTA. In cats, lymphocytes and monocytes were lower in heparinized blood compared to EDTA ( P < 0.05), whereas in horses no significant effect was seen. Regarding storage time and temperature, white and red blood cell counts, hemoglobin, and MCH were stable. Hct, MCV, and MCHC were influenced by erythrocyte swelling. Differential count remained stable for 24 hr (22°C) and nearly 72 hr (4°C) except for monocytes. The overall performance of the Sysmex XT-2000 iV was excellent and compared favorably with that of the ADVIA 2120. A special strength was the excellent detection of feline eosinophils.

scholarly journals Hematological parameters in Nile Tilápia, Oreochromis niloticus exposed to sub-letal concentrations of mercury

2007 ◽  
Vol 50 (4) ◽  
pp. 619-626 ◽  
Author(s):  
Nilton Massuo Ishikawa ◽  
Maria José Tavares Ranzani-Paiva ◽  
Julio Vicente Lombardi ◽  
Cláudia Maris Ferreira
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Oreochromis Niloticus ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Control Group ◽  
Mean Corpuscular Hemoglobin ◽  
Blood Cell Count ◽  
Corpuscular Hemoglobin ◽  
Set Up

Mercury toxicity in tilapia, Oreochromis niloticus, (Linnaeus, 1758) was investigated by the hematological parameters after long-term (14 days) exposure to various Hg concentrations (0.02, 0.002, 0.0002mg/L Hg). Test groups were set up with three replicates for each concentration, plus the control group. Blood samples were collected from six individuals for each concentration at 0, 3, 7, 10 and 14 days of exposure. The hematological parameters analyzed were: total red blood cell count (RBC), hemoglobin concentration (Hb), hematocrit (Ht), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), total white blood cell count (WBC) and differential leukocyte counts and total thrombocyte count (Tr). There were no significant differences among the mean hematological values at the different Hg concentrations indicating that Hg at the concentrations studied was not toxic to tilapia.

scholarly journals Comparison of Hematological and Biochemical Profiles of Late Pregnant and Non-Pregnant Mares

2021 ◽  
Vol 22 (1) ◽  
pp. 1-7
Author(s):  
Murat Onur Yazlik ◽  
Ezgi Dikmeoglu ◽  
Arzu Esen ◽  
Ufuk Kaya ◽  
Özgenur Kafkas ◽  
...  
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Biochemical Parameters ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Clinical Practices ◽  
Blood Cell Count ◽  
Corpuscular Hemoglobin ◽  
Mean Cell Volume ◽  
Pregnancy Status

The maternal body must adapt to fetal growth and development during the state of pregnancy for successful foal and mother. Pregnancy status as in other mammals is related to physiology and hematology. This study aimed to compare the hematology profiles and biochemical parameters of late pregnant and non-pregnant mares. Twenty-two mares were included in the study. Mares were divided into two groups according to the pregnancy status. Hematology profile and biochemical parameters were analyzed from blood samples collected from the jugular vein. All data were analyzed using Stata 12/MP4. White blood cell count, lymphocyte, red blood cell count, hemoglobin concentration, hematocrit, monocyte and percentage of monocyte were significantly lower in non-pregnant mares compared to late pregnant mares (P<0.05). No significant differences were noted in values of neutrophil, eosinophil, mean cell volume, mean corpuscular hemoglobin concentration, mean corpuscular hemoglobin, platelet, mean platelet volume, red cell distribution width in both groups (P>0.05). On the other hand, biochemical parameters analysis showed that calcium, total bilirubin, and triglyceride concentrations were greater in late pregnant mares (P<0.05). However, urea, albumin, cholesterol, and magnesium concentrations were similar among groups (P>0.05). In conclusion, the present study showed hematology profile and biochemical parameters differences in late pregnant and non-pregnant mares. These values could be useful in clinical practices to assess the situation of late pregnant mares.

scholarly journals Blood parameter analysis and morphological alterations as biomarkers on the health of Hoplias malabaricus and Geophagus brasiliensis

2006 ◽  
Vol 49 (3) ◽  
pp. 441-448 ◽  
Author(s):  
Silvia Romão ◽  
Lucélia Donatti ◽  
Matheus O. Freitas ◽  
Josiane Teixeira ◽  
Josiana Kusma
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Hemoglobin Concentration ◽  
Parameter Analysis ◽  
Mean Corpuscular Hemoglobin ◽  
Blood Parameter ◽  
Blood Cell Count ◽  
Hoplias Malabaricus ◽  
Morphological Alterations ◽  
Geophagus Brasiliensis

This study aimed to assess the influence of the environment on fish health. Samples of Hoplias malabaricus and Geophagus brasiliensis, were collected from three different environments: area I was urban and areas II and III were rural. Analyses of red blood cell count, microhematocrit, hemoglobin concentration, white blood cell count and differential white cell count in blood smear were carried out. Mean corpuscular volume and mean corpuscular hemoglobin concentration were calculated. To analyze morphological alterations, gills, liver, kidney and gonads were submitted to routine histological processing. Individuals collected from area III had slightly lower blood indices than collected from area I . Severe kidney changes, degeneration of and crystallization within kidney tubules were observed. In area I, crystallization was observed in 92% of the specimens of G. brasiliensis. These results suggested that such alterations were related with poor water circulation in the place.

scholarly journals Hematology values of captive giant anteaters (Myrmecophaga tridactyla) and collared anteaters (Tamandua tetradactyla)

2013 ◽  
Vol 33 (4) ◽  
pp. 557-560 ◽  
Author(s):  
Thaís C. Sanches ◽  
Flávia R. Miranda ◽  
Alice S. Oliveira ◽  
Eliana R. Matushima
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Hemoglobin Concentration ◽  
Relevant Information ◽  
Disease Diagnosis ◽  
Sao Paulo ◽  
Mean Corpuscular Hemoglobin ◽  
São Paulo ◽  
Blood Cell Count ◽  
Corpuscular Hemoglobin

Hematological results are reported for 13 giant anteaters (Myrmecophaga tridactyla) and 13 collared anteaters (Tamandua tetradactyla). Animals were captive-reared adults held at the Fundação Parque Zoológico de São Paulo (São Paulo, SP, Brazil) and Parque Zoológico Municipal Quinzinho de Barros (Sorocaba, SP, Brazil), and were considered healthy on physical examination. Examined parameters included red blood cell count, white blood cell count, hematocrit, hemoglobin concentration, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, total plasmatic protein and differential leukocyte counts. Also, a survey for hemoparasites was done and none was observed in thin blood smears. The results were generally similar to those previously reported in the exiguous literature for these species, providing further reference data for the interpretation of laboratory results besides health monitoring, assisting early disease diagnosis and providing relevant information for conservation programs for these species.

Performance Evaluation of Samsung LABGEOHC10 Hematology Analyzer

2014 ◽  
Vol 138 (8) ◽  
pp. 1077-1082 ◽  
Author(s):  
Il Joong Park ◽  
Sunhyun Ahn ◽  
Young In Kim ◽  
Seon Joo Kang ◽  
Sung Ran Cho
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
University Hospital ◽  
Mean Corpuscular Hemoglobin ◽  
Monocyte Count ◽  
Complete Blood Cell Count ◽  
Blood Cell Count ◽  
Cell Parameters ◽  
Wide Range ◽  
Hematology Analyzer

Context.—The Samsung LABGEOHC10 Hematology Analyzer (LABGEOHC10) is a recently developed automated hematology analyzer that uses impedance technologies. The analyzer provides 18 parameters including 3-part differential at a maximum rate of 80 samples per hour. Objective.—To evaluate the performance of the LABGEOHC10. Design.—We evaluated precision, linearity, carryover, and relationship for complete blood cell count parameters between the LABGEOHC10 and the LH780 (Beckman Coulter Inc) in a university hospital in Korea according to the Clinical and Laboratory Standards Institute guidelines. Sample stability and differences due to the anticoagulant used (K2EDTA versus K3EDTA) were also evaluated. Results.—The LABGEOHC10 showed linearity over a wide range and minimal carryover (&lt;1%) for white blood cell, hemoglobin, red blood cell, and platelet parameters. Correlation between the LABGEOHC10 and the LH780 was good for all complete blood cell count parameters (R &gt; 0.92) except for mean corpuscular hemoglobin concentration. The bias estimated was acceptable for all parameters investigated except for monocyte count. Most parameters were stable until 24 hours both at room temperature and at 4°C. The difference by anticoagulant type was statistically insignificant for all parameters except for a few red cell parameters. Conclusions.—The accurate results achievable and simplicity of operation make the unit recommendable for small to medium-sized laboratories.

scholarly journals Development of Novel Continuous and Interval Exercise Programs by Applying the FITT-VP Principle in Dogs

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
H. S. Lee ◽  
S. H. Lee ◽  
J. W. Kim ◽  
Y. S. Lee ◽  
B. C. Lee ◽  
...  
Keyword(s):  
Exercise Training ◽  
Blood Cell ◽  
Cell Count ◽  
Mean Corpuscular Hemoglobin ◽  
Reference Ranges ◽  
Blood Cell Count ◽  
Corpuscular Hemoglobin ◽  
Exercise Programs ◽  
Interval Exercise ◽  
Essential Components

Although proper exercise training induces positive physiological effects, improper exercise can lead to injury, fatigue, and poor performance. The frequency, intensity, time/duration, type, volume, and progression (FITT-VP) are the essential components of exercise training to maintain or improve physical fitness and health. The purpose of this study was to develop specific exercise programs by applying the FITT-VP principle and to examine the effects on heart rate (HR) and hematological and biochemical parameters in dogs. The healthy male Beagles (n = 4) included in this study performed continuous and interval exercises, comprising 12 protocols. The HR monitoring elicited an affirmative response to activities but varied depending on the protocols. The hematologic parameters (e.g., red blood cell count, white blood cell count, hemoglobin, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration) were within the reference ranges both before and after exercise. The creatine kinase level significantly increased, and the cholesterol level decreased after exercises. In conclusion, the continuous and interval exercise program elicits an appropriate HR reaction, has no adverse effects on the serum parameters, and provides valuable insight for healthcare in dogs.

scholarly journals Vitamin-C Treated Haematology Assessment of Swiss Albino Mice (Mus musculus)

2020 ◽  
Vol 11 (2) ◽  
pp. 9044-9050
Keyword(s):  
Vitamin C ◽  
Blood Cell ◽  
Cell Count ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
The Body ◽  
Blood Cell Count ◽  
Swiss Albino Mice ◽  
Positive Effects ◽  
Albino Mice

Vitamin C is an essential dietary supplement that plays a vital role in battling health conditions. The antioxidant has positive effects on the functioning of the body. The purpose of this study is to investigate the outcome of varying concentrations of Vitamin C on the hematological parameters of Swiss Albino mice. Four albino mice were separated and accommodated in similar caging conditions. Three of them were orally administered with 250mg/kg, 500mg/kg, and 1000mg/kg Vitamin C powder, along with their diet. The fourth one was kept as control, in order to provide a standard value for future comparison. After 30 days of administration of the Vitamin C, the red blood cell count, the white blood cell count, the hemoglobin concentration, the platelet cell count, and the packed cell volume of the blood samples were tested.

Sign in / Sign up

Export Citation Format

Share Document