scholarly journals Preliminary safety evaluation of n-butanol from the collagen extraction process and of collagen extract from Oreochromis niloticus (tilapia) skin oriented for dermocosmetics

2021 ◽  
Vol 18 (1) ◽  
pp. 123
Author(s):  
Linda Sánchez-Tuest ◽  
Nataly Reátegui-Pinedo ◽  
David Salirrosas ◽  
Ana Lucía Morocho-Jácome ◽  
Fernanda Daud Sarruf ◽  
...  
Keyword(s):  
Oreochromis Niloticus ◽  
Blood Cells ◽  
Human Peripheral Blood ◽  
White Blood Cells ◽  
Treatment Phase ◽  
Chemical Compounds ◽  
Extraction Process ◽  
Residual Water ◽  
Allium Test ◽  
Root Cells

Obtaining new cosmetic ingredients, mainly from sustainable sources, as novel excipients or even active compounds is noteworthy for the cosmetic industry to enhance new innovative dermocosmetics. Thus, it is essential to establish the safety of these new ingredients to avoid adverse events, mainly those associated with clastogenic effects from the chemical compounds used for collagen extraction. In this study, we evaluated solutions of chemical compounds used in the collagen extraction process from tilapia skin (Oreochromis niloticus). The cytotoxic and genotoxic effects of the solutions used in the collagen extraction process were 10.0, 1.0, 0.5, and 0.1% n-butanol. Solutions were evaluated by the Allium test and the comet assay in peripheral white blood cells. The residual water from the final skin wash in the pre-treatment phase and the 0.5% lyophilized collagen extract were also investigated. The absence of cytotoxic and genotoxic activity was demonstrated in the collagen extract, despite the fact that n-butanol showed DNA damage, both in the root cells of Allium cepa and in the white blood cells of human peripheral blood. Therefore, we note the necessity to carry out genotoxicity tests to guarantee the absence of contaminants in the collagen extract for cosmetic purposes

scholarly journals IMMUNOLOGICAL UNRESPONSIVENESS TO SENSITIZATION WITH SIMPLE CHEMICAL COMPOUNDS

1963 ◽  
Vol 118 (6) ◽  
pp. 1021-1035 ◽  
Author(s):  
Jack R. Battisto ◽  
Merrill W. Chase
Keyword(s):  
Specific Antibody ◽  
Blood Cells ◽  
Guinea Pigs ◽  
White Blood Cells ◽  
Chemical Compounds ◽  
Lymphoid Cells ◽  
Delayed Type Hypersensitivity ◽  
Simple Chemical ◽  
Picryl Chloride

Guinea pigs fed picryl chloride to induce specific immunologic unresponsiveness cleared small amounts of venously infused antipicryl antibody at a rate equal to that of normal guinea pigs. Catabolism of passively administered picryl-specific antibody did not alter the unresponsive state of picryl chloride-fed guinea pigs or the responsive state of normal guinea pigs. Lymphoid cells of picryl chloride immunized guinea pigs produced equal amounts of picryl-specific antibody in picryl chloride-fed and normal animals. Allergen-fed guinea pigs remained unresponsive to attempted sensitization with the allergen in excess of 10 months after the final feeding, though some became feebly sensitive between 9 and 11 months. Second attempts to make unresponsive animals hypersensitive were unsuccessful. White blood cells of guinea pigs unresponsive to picryl chloride were unable to transfer delayed-type hypersensitivity for picryl chloride to normal recipients yet readily transferred tuberculin hypersensitivity.

scholarly journals Effect of dietary fluted pumpkin (Telfairia occidentalis) extract on growth performance, body composition and haematological parameters of Nile tilapia (Oreochromis niloticus Linnaeus)

2014 ◽  
Vol 2 (3) ◽  
pp. 203 ◽  
Author(s):  
Adekunle Ayokanmi Dada ◽  
Anike Dolapo Abiodun
Keyword(s):  
Growth Rate ◽  
Body Composition ◽  
Growth Performance ◽  
Oreochromis Niloticus ◽  
Blood Cells ◽  
Nile Tilapia ◽  
White Blood Cells ◽  
Feed Utilization ◽  
Fluted Pumpkin ◽  
Nile Tilapia Oreochromis Niloticus

The effect of dietary fluted pumpkin extract on growth, body composition and haematological profile was investigated in Nile tilapia Oreochromis niloticus. Fingerlings of about 5.23-5.44 g were fed diets supplemented with four concentrations ((2.5, 5.0, 7.5 and 10.0 gkg−1) of fluted pumpkin extract powder for eight weeks. Fish fed supplemented diets showed significantly improved growth performance and feed utilization over the control (0 gkg−1 fluted pumpkin extract powder) treatment. The highest specific growth rate (0.79±0.10% per day) and best food conversion ratio (0.98±0.14) were obtained in fish fed 2.5 gkg−1 fluted pumpkin extract powder diet. No differences occurred in fish carcass moisture, protein or crude lipid content among the treatments (p>0.05). Similarly no differences occurred in white blood cells among the treatments (p>0.05) but there were greater improvement in the white blood cells of fish fed on dietary fluted pumpkin extract powder compared to the fish fed the control diet. The results suggest that dietary supplementation with fluted pumpkin extract powder improved growth rate, feed utilization, white blood cells and survival of Nile tilapia O. niloticus fingerlings.

scholarly journals Sex-Related Differences in Hematological Parameters and Organosomatic Indices of Oreochromis niloticus Exposed to Aflatoxin B1 Diet

Scientifica ◽  
2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Esther Marijani ◽  
Johnson Nasimolo ◽  
Emmanuel Kigadye ◽  
Gbemenou Joselin Benoit Gnonlonfin ◽  
Sheila Okoth
Keyword(s):  
Body Weight ◽  
Oreochromis Niloticus ◽  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Control Group ◽  
Feeding Experiment ◽  
Initial Body Weight ◽  
Male And Female ◽  
Males And Females

A 24-week feeding experiment was conducted to assess whether males and females of Oreochromis niloticus exhibit differences in their hematological responses and organosomatic indices to dietary AFB1 contamination. Triplicate groups of O. niloticus (initial body weight: 24.1 ± 0.6 g) were fed with four diets (Diets 1 to 4) containing 0, 20, 200, and 2,000 μg AFB1 kg−1. A significant decrease (P<0.05) in hemoglobin (Hb), red blood cells (RBC), and hematocrit (Hct) was observed in AFB1 exposure groups, with the lowest levels recorded in the 2000 μg AFB1 kg−1 treatment. A significant increase in mean white blood cells (WBC), neutrophils, and lymphocytes was observed in AFB1 exposure groups. No sex-related differences in RBC, WBC, lymphocytes, monocytes, and neutrophils levels were observed. However, hemoglobin and hematocrit values for female O. niloticus were significantly lower than those for male O. niloticus. Organosomatic indices showed that the relative liver, kidney, and spleen weights were significantly higher (P<0.05) in the AFB1 supplemented group than in the control group. However, the effect of aflatoxin on organosomatic indices does not depend on sex but rather depends on the dose of aflatoxin in the diet. These results provide useful information for monitoring changes in the health status of male and female O. niloticus.

Inactivation of Human White Blood Cells in Red Blood Cell Products Using the MIRASOL® System for Whole Blood.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 2897-2897
Author(s):  
Loren D. Fast ◽  
Susanne Marschner ◽  
Gilbert DiLeone ◽  
Suzann Doane ◽  
Christy Fitzpatrick ◽  
...  
Keyword(s):  
Immune Responses ◽  
T Cell Activation ◽  
Whole Blood ◽  
Blood Cells ◽  
Cell Activation ◽  
Mononuclear Cells ◽  
Protein Quality ◽  
Human Peripheral Blood ◽  
White Blood Cells ◽  
Blood Products

Abstract Transfusion of blood products containing white blood cells (WBC) can result in the induction of immune responses that can negatively impact the recipient. An approach that would mitigate these consequences would be beneficial. Previous studies had shown that exposure of platelet concentrates to light in the presence of riboflavin was able to inhibit immune responses mediated by WBC. To make this protocol more widely applicable the effect of treating whole blood units with riboflavin and varying amounts of light was tested. Human peripheral blood mononuclear cells were purified by Ficoll-Hypaque discontinuous centrifugation from aliquots of nonleukoreduced whole blood units that were untreated or exposed to Mirasol treatment using varying light dosages. Viability and phenotype of treated cells was unchanged compared to untreated controls. The results showed that exposure of whole blood units to 33J/mL red blood cells (RBC) UV light in the presence of riboflavin completely inhibited proliferation of WBC in response to polyclonal stimulators such as phytohemagglutinin, and anti-CD3/CD28 or to allogeneic stimulator cells in a mixed lymphocyte culture (MLC). Additional assays showed that treated WBC were unable to induce proliferation of normal responder cells in an MLC. Treated cells did not produce inflammatory or TH1/TH2 cytokines when stimulated with lipopolysaccharide for 24 hours or anti-CD3/CD28 for 72 hours. In addition, treatment was found to inhibit T cell activation as evidenced by the lack of CD69 expression in treated compared to untreated control cells when incubated with phorbol 12-myristate 13-acetate. These treatment conditions did not induce crossmatch incompatibility. Methemoglobin levels and hemolysis in RBC units stayed below 1% during storage for 42 days in AS-3. Platelet and plasma units separated from whole blood after treatment showed acceptable cell and protein quality over 5 days in storage or as fresh frozen plasma, respectively. In summary, Mirasol treatment was able to functionally inactivate WBC in whole blood products without adversely affecting the quality of the RBC, platelets and plasma. This technique offers potential means to achieve inactivation of WBC in whole blood units that can subsequently be separated into RBC, platelet and plasma components.

EVALUACIÓN DE LA GENOTOXICIDAD DE UNA SOPA INSTANTÁNEA COMERCIAL UTILIZANDO LA PRUEBA DE MICRONUCLEOS EN VICIA FABA Y RATÓN CD-1

2014 ◽  
Vol 7 ◽  
Author(s):  
Saúl Flores-Maya ◽  
Raul Ríos Torres ◽  
Sandra Gómez-Arroyo ◽  
Arturo Rosas Cipriano ◽  
Norberto Alarcón Herrera ◽  
...  
Keyword(s):  
Vicia Faba ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Statistical Significance ◽  
Chemical Compounds ◽  
Food Product ◽  
Peripheral Blood Cells ◽  
Market Demand ◽  
Root Cells ◽  
Mutagenic Effects

Due to the high market demand that instant soup has among the Mexican population, the question arose whether it may cause any mutagenic effects after its consumption. The aim of this study was to analyze the cyto-genotoxic effects caused after the exposure of this food product compounds to meristemal root cells of Vicia faba and to peripheral blood cells of CD-1 mice. To determine this effect, it was performed both analysis of micronuclei as well as toxicity degree by using these two biological models. The interpretation of our statistical significance tests (ANOVA and Dunnett’s multiple comparison p&lt;0.05), allowed us to conclude that nor the soup chemical compounds neither the packaging induce clastogenic damage but they cause a slight delay on cell division to V. faba; nevertheless the compounds can cause clastogenic damage and cytotoxicity to peripheral blood cells of CD-1 mice.

Ultrastructural changes in murine lymphoma cells exposed to ultraviolet-A radiation

1991 ◽  
Vol 49 ◽  
pp. 104-105
Author(s):  
Delma P. Thomas ◽  
Dianne E. Godar
Keyword(s):  
Medical Devices ◽  
Blood Cells ◽  
White Blood Cells ◽  
Consumer Products ◽  
Ultrastructural Changes ◽  
Ultraviolet A ◽  
Uv Spectrum ◽  
Lymphoma Cells ◽  
Murine Lymphoma ◽  
Transmission Electron

Ultraviolet radiation (UVR) from all three waveband regions of the UV spectrum, UVA (320-400 nm), UVB (290-320 nm), and UVC (200-290 nm), can be emitted by some medical devices and consumer products. Sunlamps can expose the blood to a considerable amount of UVR, particularly UVA and/or UVB. The percent transmission of each waveband through the epidermis to the dermis, which contains blood, increases in the order of increasing wavelength: UVC (10%) < UVB (20%) < UVA (30%). To investigate the effects of UVR on white blood cells, we chose transmission electron microscopy to examine the ultrastructure changes in L5178Y-R murine lymphoma cells.

The Effect of Red Blood Cells on the ADP-induced Aggregation of Human Platelets in Flow Through Tubes

1990 ◽  
Vol 63 (01) ◽  
pp. 112-121 ◽  
Author(s):  
David N Bell ◽  
Samira Spain ◽  
Harry L Goldsmith
Keyword(s):  
Platelet Aggregation ◽  
Shear Rate ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Platelet Rich Plasma ◽  
Mean Transit Time ◽  
White Blood Cells ◽  
Aggregate Size ◽  
Human Platelets ◽  
Induced Aggregation

SummaryThe effect of red blood cells, rbc, and shear rate on the ADPinduced aggregation of platelets in whole blood, WB, flowing through polyethylene tubing was studied using a previously described technique (1). Effluent WB was collected into 0.5% glutaraldehyde and the red blood cells removed by centrifugation through Percoll. At 23°C the rate of single platelet aggregtion was upt to 9× greater in WB than previously found in platelet-rich plasma (2) at mean tube shear rates Ḡ = 41.9,335, and 1,920 s−1, and at both 0.2 and 1.0 µM ADP. At 0.2 pM ADP, the rate of aggregation was greatest at Ḡ = 41.9 s−1 over the first 1.7 s mean transit time through the flow tube, t, but decreased steadily with time. At Ḡ ≥335 s−1 the rate of aggregation increased between t = 1.7 and 8.6 s; however, aggregate size decreased with increasing shear rate. At 1.0 µM ADP, the initial rate of single platelet aggregation was still highest at Ḡ = 41.9 s1 where large aggregates up to several millimeters in diameter containing rbc formed by t = 43 s. At this ADP concentration, aggregate size was still limited at Ḡ ≥335 s−1 but the rate of single platelet aggregation was markedly greater than at 0.2 pM ADP. By t = 43 s, no single platelets remained and rbc were not incorporated into aggregates. Although aggregate size increased slowly, large aggregates eventually formed. White blood cells were not significantly incorporated into aggregates at any shear rate or ADP concentration. Since the present technique did not induce platelet thromboxane A2 formation or cause cell lysis, these experiments provide evidence for a purely mechanical effect of rbc in augmenting platelet aggregation in WB.

White blood cells levels and PCOS: direct and indirect relationship with insulin resistance, but not with hyperandogenemia

2013 ◽  
Author(s):  
Olga Papalou ◽  
Sarantis Livadas ◽  
Athanasios Karachalios ◽  
Nektarios Benetatos ◽  
George Boutzios ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Blood Cells ◽  
White Blood Cells ◽  
Indirect Relationship

Endogenous Heparinoids Detected by anti-Xa Activity are Present in Blood during Acute Variceal Bleeding in Cirrhosis. A Prospective Study

2014 ◽  
Vol 23 (2) ◽  
pp. 187-194 ◽  
Author(s):  
Christos Triantos ◽  
Emmanuel Louvros ◽  
Maria Kalafateli ◽  
Anne Riddell ◽  
Ulrich Thalheimer ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Variceal Bleeding ◽  
Blood Cells ◽  
Venous Pressure ◽  
White Blood Cells ◽  
International Normalized Ratio ◽  
Ulcer Bleeding ◽  
Factor X ◽  
Packed Red Blood Cells ◽  
Bacterial Peritonitis

Background & Aims: Endogenous heparinoids have been detected by thromboelastography and quantified by clotting based anti-Xa activity assays in patients with cirrhosis, but their presence in variceal bleeding has not been established yet.Methods: Clotting based anti-Xa activity was measured in A) 30 cirrhotics with variceal bleeding, B) 15 noncirrhotics with peptic ulcer bleeding, C) 10 cirrhotics without infection or bleeding, and D) 10 cirrhotics with hepatocellular carcinoma (HCC).Results: Anti-Xa activity was not detected in ulcer bleeders or in cirrhotics without infection or bleedingbut was present in seven (23%) variceal bleeders (median levels: 0.03 u/mL (0.01-0.07)) and was quantifiable for 3 days in six of seven patients. Four of seven variceal bleeders with anti-Xa activity present had HCC (p=0.023). Age, creatinine, platelet count and total infections the second day from admission were significantly correlated with the presence of measureable anti-Xa levels (p=0.014, 0.032, 0.004 and 0.019, respectively). In the HCC group, anti-Xa activity was present in three patients (30%) [median levels: 0.05 u/mL (0.01-0.06)].Conclusions: In this study, variceal bleeders and 30% of the patients with HCC had endogenous heparinoids that were detected by a clotting based anti-Xa activity assay, whereas there was no anti Xa activity present in patients with cirrhosis without infection, or bleeding or HCC, nor in those with ulcer bleeding. Thus, the anti-Xa activity is likely to be a response to bacterial infection and/or presence of HCC in cirrhosis.List of abbreviations: AFP, alpha-fetoprotein; aPTT, activated partial thromboplastin time; CP, Child-Pugh; FXa, activated factor X; GAGS, glycosaminoglycans; Hb, hemoglobin; HCC, hepatocellular carcinoma; HVPG, hepatic venous pressure gradient; INR, International normalized ratio; LMWHs, low molecular weight heparins; MELD, Model for End-stage Liver Disease; PPP, platelet-poor plasma; PRBC, packed red blood cells; PT, prothrombin time; SBP, sponataneous bacterial peritonitis; TEG, thromboelastography; WBC, white blood cells.

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