Analysis of IL-10 in HIV-1 patients with chronic periodontitis in northern Brazil

2017 ◽  
Vol 16 ◽  
pp. 1-9
Author(s):  
Silvio Augusto Fernandes de Menezes ◽  
Tatiany Oliveira de Alencar Menezes ◽  
Tânia Maria de Souza Rodrigues ◽  
Brenna Magdalena Lima Nogueira ◽  
Ricardo Roberto de Souza Fonseca
Keyword(s):  
Viral Load ◽  
Chronic Periodontitis ◽  
Gingival Crevicular Fluid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Clinical Attachment Level ◽  
Probing Depth ◽  
Northern Brazil ◽  
Crevicular Fluid ◽  
Attachment Level ◽  
Hiv 1

Aim: The objective of this study was to investigate the levels of IL-10 in the gingival crevicular fluid in HIV-1 positive patients with chronic periodontitis and to compare with HIV-1 negative patients with chronic periodontitis, also to correlate clinical periodontal parameters, viral load and count of CD4+ and CD8+ lymphocytes (LTCD4+ and LTCD8+). Methods: 33 patients were selected and splitted into two groups: 16 HIV-1 positive patients and 17 HIV-1 negative patients and all with chronic periodontitis. The clinical periodontal parameters recorded were: Probing Depth (PD) and Clinical Attachment Level (CAL); the sistemical parameters LTCD4+, LTCD8+ and viral load were analized by the gingival crevicular fluid collected from all patients. Enzyme-linked immunosorbent assay (ELISA) was used to determine the concentrations of Interleukin (IL)-10. For the statistical analysis the Student t, Mann-Whitney and Spearman tests were performed. IL-10 levels were significantly lower in both patients groups. Results: There was statistical difference betwen groups for probing depth (p=0.015) and clinical attachment level (p=0.011), no significant correlation was found among the analyzed variables. Conclusion: The IL-10 levels in HIV-1 positive patients had no influence in periodontal and medical parameters.

scholarly journals Non-Surgical Periodontal Therapy with Adjunctive Amoxicillin/Metronidazole or Metronidazole When No Aggregatibacter actinomycetemcomitans Is Detected—A Randomized Clinical Trial

Antibiotics ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 686
Author(s):  
Holger F. R. Jentsch ◽  
Martin Dietrich ◽  
Sigrun Eick
Keyword(s):  
Clinical Trial ◽  
Randomized Clinical Trial ◽  
Gingival Crevicular Fluid ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Periodontal Therapy ◽  
Clinical Attachment Level ◽  
Probing Depth ◽  
Crevicular Fluid ◽  
Systemic Antibiotics ◽  
Attachment Level

Background: The aim was to compare two different systemic antibiotics regimens adjunctive to non-surgical periodontal therapy when Aggregatibacter actinomycetemcomitans was not detected in the subgingival biofilm. Methods: A total of 58 patients with periodontitis and with no A. actinomycetemcomitans in the subgingival biofilm were treated with full-mouth subgingival instrumentation and either metronidazole (MET; n = 29) or amoxicillin/metronidazole (AMX/MET; n = 29). Probing depth (PD), clinical attachment level (CAL) and bleeding on probing (BOP) were recorded at baseline, as well as after three and six months. Subgingival biofilm and gingival crevicular fluid were collected and analyzed for major periodontopathogens and biomarkers. Results: PD, CAL and BOP improved at 3 and 6 months (each p < 0.001 vs. baseline) with no difference between the groups. Sites with initial PD ≥ 6 mm also improved in both groups after 3 and 6 months (p < 0.001) with a higher reduction of PD in the AMX/MET group (p < 0.05). T. forsythia was lower in the AMX/MET group after 3 months (p < 0.05). MMP-8 and IL-1β were without significant changes and differences between the groups. Conclusion: When A. actinomycetemcomitans was not detected in the subgingival biofilm, the adjunctive systemic use of amoxicillin/metronidazole results in better clinical and microbiological outcomes of non-surgical periodontal therapy when the application of systemic antibiotics is scheduled.

scholarly journals Effect of Periodontal Surgery on Osteoprotegerin Levels in Gingival Crevicular Fluid, Saliva, and Gingival Tissues of Chronic Periodontitis Patients

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Sandy H. S. Hassan ◽  
Mahmoud I. El-Refai ◽  
Noha A. Ghallab ◽  
Rehab Fawzy Kasem ◽  
Olfat G. Shaker
Keyword(s):  
Chronic Periodontitis ◽  
Gingival Crevicular Fluid ◽  
Prognostic Biomarker ◽  
Bone Destruction ◽  
Gingival Index ◽  
Pocket Depth ◽  
Clinical Attachment Level ◽  
Crevicular Fluid ◽  
Open Flap Debridement ◽  
Attachment Level

Objectives. This study was undertaken to investigate the OPG profiles in gingival crevicular fluid (GCF), saliva, and gingival tissues of chronic periodontitis (CP) patients in response to open flap debridement (OFD).Subjects and Methods. The study included 30 subjects divided into 2 groups: 20 CP patients and 10 periodontally healthy subjects. Plaque index, gingival index, pocket depth, and clinical attachment level measurements were recorded for all subjects. GCF, salivary, and gingival samples were collected from all 30 subjects at baseline and 3 and 6 month after OFD from the 20 CP patients. GCF and salivary OPG levels were assessed by ELISA assay, while OPG expression in gingival tissues was examined by immunohistochemistry.Results. GCF, salivary and gingival OPG profiles were significantly higher in control subjects compared to CP patients at baseline (P<0.001). Within CP group, OPG levels in GCF, saliva, and gingival samples showed a significant increase at 3 and 6 months after OFD (P<0.001) compared to baseline. Although OPG values increased significantly in gingival samples and insignificantly in saliva after 3 months compared to 6 months, yet GCF levels were significantly decreased.Conclusions. OPG might be considered as a diagnostic and prognostic biomarker of periodontal bone destruction. This trial is registered withNCT02160613.

scholarly journals Gingival Crevicular Fluid Levels of Prolidase and Alkaline Phosphatase in Periodontitis

2017 ◽  
Vol 21 (3) ◽  
pp. 158-161
Author(s):  
Berrak Guven ◽  
Cigdem Turer
Keyword(s):  
Alkaline Phosphatase ◽  
Gingival Crevicular Fluid ◽  
Clinical Parameters ◽  
Clinical Attachment Level ◽  
Probing Depth ◽  
Healthy Control ◽  
Periodontal Destruction ◽  
Crevicular Fluid ◽  
Fluid Levels ◽  
Attachment Level

SummaryBackground/Aim: The purpose of this study was to investigate gingival crevicular fluid (GCF) alkaline phosphatase (ALP) and prolidase levels in subjects with different periodontal status. Material and Methods: Fifteen periodontitis, fifteen gingivitis and fifteen healthy subject were included. GCF samples were collected from participants. Probing depth, clinical attachment level, gingival index was recorded. ALP and prolidase levels were determined in GCF by spectrophotometrically. Results: Higher values of ALP were found in periodontitis compared with gingivitis and healthy control (p<0.001). The values of prolidase were lower in periodontitis than healthy control (p<0.05). A statistically significant positive correlation was found between clinical parameters and ALP levels (p<0.001). There is no significant correlation between clinical parameters and prolidase levels (p>0.05). Additionally, no significant correlation was detected between ALP and prolidase (r= -0.309, p>0.05). Conclusion: Our preliminary data suggest that low prolidase level in periodontitis was not associated with ALP and clinical parameters, which represent periodontal destruction and inflammation.

scholarly journals Inpatient Evaluation of Periodontal, Esthetic and Inflammatory Parameters around Dental Implants and Natural Teeth

2013 ◽  
Vol 14 (1) ◽  
pp. 90-95 ◽  
Author(s):  
Güliz N Güncü ◽  
Gülüzar Büyüktepe ◽  
Sezen B AÕkin ◽  
Kamer Klllnç ◽  
Celal Tümer ◽  
...  
Keyword(s):  
Dental Implants ◽  
Gingival Crevicular Fluid ◽  
Gingival Index ◽  
Clinical Attachment Level ◽  
Inflammatory Parameters ◽  
Probing Depth ◽  
Natural Teeth ◽  
Fixed Prosthesis ◽  
Crevicular Fluid ◽  
Attachment Level

ABSTRACT Aim The use of endosseous dental implants (DI) has become a successful treatment alternative. However, providing periimplant tissue health and achieving a natural esthetic look are important topics in this treatment. The aim of the present study was to evaluate periodontal and esthetic parameters around DI and natural teeth (NT) and also to analyze myeloperoxidase (MPO) levels in gingival crevicular fluid (GCF) and peri-implant sulcus fluid (PISF). Materials and methods Twenty DI supported fixed prosthesis and contralateral 20 NT were enrolled to the present study. Clinical periodontal parameters (probing depth, clinical attachment level, gingival bleeding time index and gingival index) were recorded and GCF/PISF samples were obtained from mesial (mesiobuccal and mesiolingual) and distal (distobuccal and distolingual) sites of DI and NT. MPO levels were spectrophotometrically determined. Additionally clinical photographs were obtained and esthetical evaluations were performed by using Jemt papilla index. The parameters belong to DI and NT were compared and correlations were evaluated using statistical analysis. Results A total of 40 samples were evaluated. No statistically significant differences were detected between groups in all periodontal parameters and MPO levels from mesial and distal sites. Jemt papilla index scores were slightly higher in NT however, this difference was not statistically significant (p > 0.05). Total PES score were similiar in DI and NT groups. Significant correlations were detected between MPO and gingival index values as expected. Clinical significance Dental implants are satisfactory treatments, they provide patient esthetic natural looking, phonetic and masticatory functions. How to cite this article Güncü GN, Büyüktepe G, AÕkin SB, KlllnH K, Tümer C, Tözüm TF. Inpatient Evaluation of Periodontal Esthetic and Inflammatory Parameters around Dental Implants and Natural Teeth. J Contemp Dent Pract 2013;14(1):90-95.

scholarly journals Salivary Levels of IL-6 and IL-17 Could Be an Indicator of Disease Severity in Patients with Calculus Associated Chronic Periodontitis

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Husniah Batool ◽  
Ahmed Nadeem ◽  
Muhammad Kashif ◽  
Faheem Shahzad ◽  
Romeeza Tahir ◽  
...  
Keyword(s):  
Chronic Periodontitis ◽  
Alveolar Bone ◽  
Gingival Crevicular Fluid ◽  
Severe Disease ◽  
Cross Sectional Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Controls ◽  
Cross Sectional ◽  
Significant Difference ◽  
Crevicular Fluid

Background/Purpose. Chronic periodontitis is an inflammatory disease of gums that causes loss of supporting structures of teeth, that is, gingiva, periodontal ligament, cementum, and alveolar bone. Levels of various cytokines in the serum, gingival tissues, and gingival crevicular fluid in patients with chronic periodontitis have been studied, but limited data are available on the level of cytokines in saliva. Therefore, a study was designed to determine levels of salivary IL-6 and IL-17 in patients with calculus associated chronic periodontitis. Materials and Methods. It was a comparative, cross-sectional study that is comprised of 41 healthy controls and 41 calculus associated chronic periodontitis patients (CP patients). According to the degree of attachment loss, CP patients were subcategorized as mild (CAL 1-2 mm), moderate (CAL 3-4 mm), and severe (CAL > 5 mm) forms of periodontitis. Salivary levels of IL-6 and IL-17 were determined using enzyme-linked immunosorbent assay (ELISA) technique. Data was analyzed using SPSS 20.0. Results. Between healthy controls and CP patients (moderate and severe disease), a statistically significant difference was observed in the concentrations of IL-6 and IL-17. In CP patients, the highest mean ± SD of salivary IL-6 and IL-17 was observed in severe CP, followed by moderate and mild CP. Regarding level of IL-6, a statistically significant difference was observed between mild and severe disease and between moderate and severe subcategories of CP patients. Similarly, statistically significant difference was observed in the level of IL-17 between mild and moderate, mild and severe disease, and moderate and severe disease. Conclusion. The levels of salivary IL-6 and IL-17 were increased significantly in calculus associated CP patients as compared to healthy controls and these levels increased with the progression of CP. Clinical Significance. Salivary levels of IL-6 and IL-17 may help in the subcategorization of CP.

scholarly journals Gingival Crevicular Fluid Calprotectin, Osteocalcin and Cross-Linked N-Terminal Telopeptid Levels in Health and Different Periodontal Diseases

2011 ◽  
Vol 31 (6) ◽  
pp. 343-352 ◽  
Author(s):  
Sema Becerik ◽  
Beral Afacan ◽  
Veli Özgen Öztürk ◽  
Harika Atmaca ◽  
Gülnur Emingil
Keyword(s):  
Healthy Subjects ◽  
Gingival Crevicular Fluid ◽  
Periodontal Diseases ◽  
Inflammatory Marker ◽  
Aggressive Periodontitis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Probing Depth ◽  
Crevicular Fluid ◽  
First Time

Aim:The aim of the present study was to investigate gingival crevicular fluid (GCF) calprotectin, osteocalcin and cross-linked N-terminal telopeptide (NTx) levels in health along with different periodontal diseases.Material and methods:Twenty chronic periodontitis (CP), 20 generalized aggressive periodontitis (G-AgP), 20 gingivitis and 20 healthy subjects were included. Probing depth, clinical attachment level, plaque index and papillary bleeding index was recorded. GCF calprotectin, osteocalcin and NTx levels were analyzed by enzyme-linked immunosorbent assay (ELISA).Results:CP, G-AgP and gingivitis groups had higher GCF calprotectin total amount compared to healthy subjects (p< 0.008). CP and G-AgP groups had similar, but higher levels compared to gingivitis groups (p< 0.008). CP and G-AgP groups had lower GCF osteocalcin total amount compared to gingivitis and healthy groups (p< 0.008). CP group had higher GCF NTx but lower osteocalcin total amount and osteocalcin/NTx ratio than the G-AgP group (p< 0.008)Conclusions:Our results suggest that elevated GCF calprotectin levels play a role as a reliable inflammatory marker in the pathogenesis of periodontal disease. Fluctuating GCF levels of osteocalcin and NTx might point out to the abnormal bone turnover in periodontitis. Our data document for the first time the role of NTx in the pathogenesis of different periodontal diseases.

scholarly journals Arginine and glutamate levels in the gingival crevicular fluid from patients with chronic periodontitis

2008 ◽  
Vol 19 (4) ◽  
pp. 318-322 ◽  
Author(s):  
Narda Téllez ◽  
Natalia Aguilera ◽  
Belkis Quiñónez ◽  
Elizabeth Silva ◽  
Luis Enrique González ◽  
...  
Keyword(s):  
Chronic Periodontitis ◽  
Gingival Crevicular Fluid ◽  
Healthy Controls ◽  
Clinical Attachment Loss ◽  
Measure Concentration ◽  
Periodontal Tissues ◽  
Glutamate Concentration ◽  
Probing Depth ◽  
Crevicular Fluid ◽  
Post Hoc

The objectives of this study were to determine arginine and glutamate levels in the gingival crevicular fluid (GCF) of adult chronic periodontitis patients versus periodontally healthy controls, and to compare two kinds of microdialysis probes: normal and U-shaped probes. The analysis of GCF components was developed to improve the diagnosis of periodontal disease (PD). Proteolysis in the periodontal tissues increases the concentration of amino acids (aa) in the GCF and the levels of these aa may reveal PD features and stages. GCF samples were collected by microdialysis in situ from 5 periodontally affected sites (probing depth >5 mm, clinical attachment loss >3 mm) in 14 adult chronic periodontitis patients and from 14 adult periodontally healthy controls. Capillary zone electrophoresis coupled to laser induced fluorescence detection was used to measure concentration of arginine and glutamate in the GCF. Data were analyzed statistically by ANOVA and Tukey's post-hoc tests (?=0.05). Arginine concentration was increased (p<0.001) and glutamate concentration was decreased (p<0.001) in chronic periodontitis patients as compared to controls. There were no significant differences (p=0.069) between the normal and U-shaped probes. In conclusion, the increase of arginine and decrease of glutamate concentration in GCF were associated to the presence of periodontitis, and might be used as markers to recognize periodontally susceptible subjects as well as to evaluate the treatment course.

scholarly journals Transmission of Aggregatibacter actinomycetemcomitans between Brazilian women with severe chronic periodontitis and their children

2007 ◽  
Vol 18 (3) ◽  
pp. 220-224 ◽  
Author(s):  
Rodrigo Otávio Citó César Rêgo ◽  
Denise Madalena Palomari Spolidorio ◽  
Sérgio Luiz de Souza Salvador ◽  
Joni Augusto Cirelli
Keyword(s):  
Chronic Periodontitis ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Selective Medium ◽  
Child Transmission ◽  
Clinical Attachment Level ◽  
Gram Staining ◽  
Probing Depth ◽  
Polymerase Chain ◽  
Attachment Level ◽  
Transport Fluid

This study evaluated the transmission of Aggregatibacter actinomycetemcomitans (Aa) in women with severe chronic periodontitis and their children. Thirty women (mean age = 36.1±6.0 years) who were mothers of at least one child aged 7 to 16 years were enrolled. In order to investigate mother-child transmission of Aa, the children were also evaluated when their mothers were colonized by the bacterium. Subgingival plaque samples of each woman were collected from 3 sites (mean probing depth of 7.3±1.2 mm and mean clinical attachment level of 7.9±1.5 mm) and pooled in reduced transport fluid (RTF). These samples were processed, inoculated onto TSBV-agar selective medium and incubated at 37°C in microaerophilic atmosphere for 5 days. Aa was identified on the basis of colony morphology, Gram staining, catalase and oxidase reactions. Aa was found in 8 out of 30 women. Therefore, 8 children from these women (mean age= 12 ± 3.7 years) were evaluated, but Aa was found only in 2 of them. Aa strains of the two mother-child pairs were evaluated by arbitrarily-primed polymerase chain reaction (AP-PCR), although it was not found similarity between the amplitypes of each pair. No Aa transmission was found between Brazilian women with severe chronic periodontitis and their children.

scholarly journals Macrophage Migration Inhibitory Factor Levels in Gingival Crevicular Fluid, Saliva, and Serum of Chronic Periodontitis Patients

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Yveth Marlene Ortiz-García ◽  
Trinidad García-Iglesias ◽  
Gabriela Morales-Velazquez ◽  
Blanca Patricia Lazalde-Ramos ◽  
Guillermo Moisés Zúñiga-González ◽  
...  
Keyword(s):  
Migration Inhibitory Factor ◽  
Chronic Periodontitis ◽  
Macrophage Migration Inhibitory Factor ◽  
Gingival Crevicular Fluid ◽  
Clinical Signs ◽  
Inhibitory Factor ◽  
Enzyme Linked Immunosorbent Assay ◽  
Macrophage Migration ◽  
Cross Sectional ◽  
Crevicular Fluid

Chronic periodontitis (CP) is an infection that affects the teeth supporting structure. Macrophage migration inhibitory factor (MIF) is an important effector cytokine of the innate immune system. Due to its functional characteristics, MIF may be involved in the immunopathology of CP. The aim of the present study was to evaluate MIF levels in gingival crevicular fluid (GCF), saliva, and serum of CP patients. A cross-sectional study was conducted on 60 subjects divided into two groups: subjects with CP (n= 30) and periodontally healthy subjects without CP (n=30). MIF was quantified in GCF, saliva, and serum of all participants by enzyme-linked immunosorbent assay. MIF concentrations were higher in GCF, saliva, and serum in the group with CP compared with the group without CP and a higher MIF concentration was observed in GCF (p=0.001) and saliva (p=0.009) in the group with CP. MIF intragroup comparisons between fluids demonstrated significant high levels of MIF in saliva compared with GCF and serum in both study groups (p<0.05). A positive correlation was found between clinical signs and MIF concentration in GCF (p<0.05). There is an association between the MIF and the clinical signs of the disease. Therefore, MIF could have an important role in the pathology and progression of CP.

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