Objectives: Medicinal plants, either as an extract, pure compound or as a derivative, offer limitless opportunities for the discovery of new drugs. Sudan is a very rich source of medicinal plants which are used in the treatment of a wide range of diseases. Aloe sinkatana, has great potential to be developed as drug by pharmaceutical industries. The present study is undertaken to investigate the antioxidant potential of Aloe sinkatana by DPPH radical scavenging activity. In addition, the study also performed to explore the possibility of using HPLC-MS technique for the determination and analysis of Aloe sinkatana.
Methods: The extracts of Aloe sinkatana were analyzed for antioxidant activity by using DPPH free radical scavenging activity. The results indicated that the extracts showed a high effective free radical scavenging in the DPPH assay, also these extracts exhibited a noticeable antioxidant effect at low concentrations.
Results: During in vitro evaluation the antioxidant potential of methanolic extract was the highest, followed by aqueous extract in DPPH radical scavenging activity. So the methanolic extract of the plant, exhibited a great antioxidant effect at 50 μg/ml which may be attributed to high phenolic content. Therefore, methanolic extract to be a more active radical scavenger than aqueous extract. The HPLC-MS analysis had shown the methanolic extract of Aloe sinkatana to be rich in the major anthraquinones and their glucosides, which revealed 9 compounds, and also UV spectroscopy detected the presence of two flavonoids.
Conclusion: The results indicated that the extracts of Aloe sinkatana is a potential source of natural antioxidants or nutraceuticals with potential application to reduce oxidative stress with consequent health benefits. Due to stronger antioxidant potential and phytochemical composition, Aloe sinkatana could be proved as a valuable prospect in pharmaceutical formulations by taking part in the antioxidant defense system against generation of free radicals.
Peer Review History:
Received 26 March 2019; Revised 15 April; Accepted 4 May, Available online 15 May 2020
UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency.
Received file
Average Peer review marks at initial stage: 4.5/10
Average Peer review marks at publication stage: 7.0/10
Reviewer(s) detail:
Name: Prof Cyprian Ogbonna ONYEJI
Affiliation: Obafemi Awolowo University, Ile-Ife, Nigeria
E-mail: [email protected]
Name: Dr. Gehan Fawzy Abdel Raoof Kandeel
Affiliation: Pharmacognosy Department, National Research Centre, Dokki, 12622, Giza, Egypt
E-mail: [email protected]
Name: Dr. Nyunaï Nyemb
Affiliation: Ministry of Scientific Research and Innovation of Cameroon
E-mail: [email protected]
Comments of reviewer(s):
Similar Articles:
ANTIHYPERGLYCEMIC AND ANTI-OXIDANT POTENTIAL OF ETHANOL EXTRACT OF VITEX THYRSIFLORA LEAVES ON DIABETIC RATS
Induction and Proliferation of Callus in the Flower Stalk of Calanthe discolor Lindl. and its Antioxidant Properties
