scholarly journals PROFIL GEN GROWTH HORMONE (GH) SAPI HASIL PERSILANGAN MADURA DAN LIMOUSIN DENGAN METODE PCR-RFLP

2020 ◽  
Vol 8 (1) ◽  
pp. 43
Author(s):  
Ghea Aquatica Puteri ◽  
Budi Utomo S ◽  
Roesno Darsono
Keyword(s):  
Growth Hormone ◽  
Dna Extraction ◽  
Fragment Length ◽  
Base Pair ◽  
Gene Profile ◽  
Pcr Rflp ◽  
Gh Gene ◽  
Base Pair Fragment ◽  
Cattle Blood ◽  
Pair Fragment

The purpose of this research was to find out the Growth Hormone (GH) gene profile of the cross breeding between Madura cattle and Limousin cattle (Madrasin). Sampl in the form of cattle blood for this research was obtained from 14 Madrasin cattles in the area of Bangkalan, Madura, East Java. DNA extraction was performed then to provide the result for PCR RFLP, which then indicated that Madrasin cattle’s GH gene profile has 432 base pair fragment length and the RFLP result indicated that Madrasin cattle’s GH genetic was cut off into 180 base pair, 250 base pair, 300 base pair, and 400 base pair. Moreover, there was no V genetic to be found in GH genetic of Madura cattle.

Two systems of glucose repression of the GAL1 promoter in Saccharomyces cerevisiae

1990 ◽  
Vol 10 (9) ◽  
pp. 4757-4769
Author(s):  
J S Flick ◽  
M Johnston
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Base Pair ◽  
Glucose Repression ◽  
Gene Products ◽  
Gal1 Promoter ◽  
Base Pair Fragment ◽  
Common Signal ◽  
Multiple Sequences ◽  
Pair Fragment ◽  
Gal1 Gene

Expression of the GAL1 gene in Saccharomyces cerevisiae is strongly repressed by growth on glucose. We show that two sites within the GAL1 promoter mediate glucose repression. First, glucose inhibits transcription activation by GAL4 protein through UASG. Second, a promoter element, termed URSG, confers glucose repression independently of GAL4. We have localized the URSG sequences responsible for glucose repression to an 87-base-pair fragment located between UASG and the TATA box. Promoters deleted for small (20-base-pair) segments that span this sequence are still subject to glucose repression, suggesting that there are multiple sequences within this region that confer repression. Extended deletions across this region confirm that it contains at least two and possibly three URSG elements. To identify the gene products that confer repression upon UASG and URSG, we have analyzed glucose repression mutants and found that the GAL83, REG1, GRR1, and SSN6 genes are required for repression mediated by both UASG and URSG. In contrast, GAL82 and HXK2 are required only for UASG repression. A mutation designated urr1-1 (URSG repression resistant) was identified that specifically relieves URSG repression without affecting UASG repression. In addition, we observed that the SNF1-encoded protein kinase is essential for derepression of both UASG and URSG. We propose that repression of UASG and URSG is mediated by two independent pathways that respond to a common signal generated by growth on glucose.

scholarly journals Association of growth hormone (GH) gene polymorphism with growth and carcass in Sumba Ongole (SO) cattle

2017 ◽  
Vol 42 (3) ◽  
pp. 153 ◽  
Author(s):  
P. P. Agung ◽  
S. Anwar ◽  
W. P. B. Putra ◽  
M. S. A. Zein ◽  
A. S. Wulandari ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Gene Polymorphism ◽  
Fragment Length Polymorphism ◽  
Growth Parameters ◽  
Rflp Analysis ◽  
Cattle Breeding ◽  
Pcr Rflp ◽  
Gh Gene ◽  
Polymerase Chain ◽  
Dressing Percentage

A study was conducted to identify the polymorphism in the intron 3 of the Growth Hormone (GH) gene and also to evaluate the association of the GH gene polymorphism with growth parameters and dressing percentage in the Sumba Ongole (SO) cattle. A total of 267 individual DNA samples were used in the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis. The SO cattle growth parameters data (n=44) including birth weight (BW), weaning weight at 205 days of age (WW205), yearling weight at 365 days of age (YW365) and also dressing percentage (DP) (n=122) were investigated in this study. There were three genotypes (AA, AB, and BB) of the GH gene based on the PCR-RFLP analysis with allele frequency was 0.87 and 0.13 for A allele and B allele respectively. The highest genotype frequency in the SO cattle is AA (0.76) and the lowest is BB (0.02). The Heterozygosity Observed (Ho) value in the SO cattle population is 0.23 and Polymorphism Information Content (PIC) value is 0.20. Therefore, the genetic diversity in the SO cattle based on the GH gene polymorphism is quite low. There is no association (P>0.05) in BW, WW205, YW365, and DP with genotypes of the GH gene. As the result, the GH gene in this study cannot be used as a genetic marker in the SO cattle breeding program.

Sequence analysis and PCR-RFLP of growth hormone (GH) gene in Vembur and Kilakarsal breeds of sheep

2017 ◽  
Vol 23 (2) ◽  
pp. 148
Author(s):  
M. Seevagan ◽  
V. Jeichitra ◽  
R. Rajendran ◽  
K.G. Tirumurugaan
Keyword(s):  
Growth Hormone ◽  
Sequence Analysis ◽  
Pcr Rflp ◽  
Gh Gene

scholarly journals Characterization of the human p53 gene promoter.

1989 ◽  
Vol 9 (5) ◽  
pp. 2163-2172 ◽  
Author(s):  
S P Tuck ◽  
L Crawford
Keyword(s):  
Base Pair ◽  
Accurate Determination ◽  
Gene Promoter ◽  
Simian Virus 40 ◽  
P53 Gene ◽  
Simian Virus ◽  
Stem Loop ◽  
Base Pair Fragment ◽  
Exon 1 ◽  
Pair Fragment

Transcriptional deregulation of the p53 gene may play an important part in the genesis of some tumors. We report here an accurate determination of the transcriptional start sites of the human p53 gene and show that the majority of p53 mRNA molecules do not contain a postulated stem-loop structure at their 5' ends. Recombinant plasmids of the human p53 promoter-leader region fused to the bacterial chloramphenicol acetyltransferase gene (cat) were constructed. After transfection into rodent or human cells, a 350-base-pair fragment spanning the promoter region conferred 4% of the CAT activity mediated by the simian virus 40 early promoter/enhancer. We monitored the efficiency with which 15 3' and 5' promoter deletion constructs initiated transcription. Our results show that an 85-base-pair fragment, previously thought to have resided in exon 1, is all that is required for full promoter activity.

scholarly journals Genetic Polymorphism of Growth Hormone Gene Exon-4 in Surti and Mehsani Goats by PCR- RFLP

2018 ◽  
Vol 14 (1) ◽  
Author(s):  
Jyotishree Bayan ◽  
Vishnu Kharadi ◽  
Umed Ramani ◽  
Mamta Janmeda ◽  
Kuldeep Tyagi ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Fragment Length Polymorphism ◽  
Allelic Frequency ◽  
Growth Hormone Gene ◽  
Chain Reaction ◽  
Pcr Rflp ◽  
Gh Gene ◽  
Polymerase Chain ◽  
Exon 4 ◽  
Gene Exon

The present investigation was planned to study growth hormone (GH) gene exon-4 polymorphism using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) in Surti and Mehsani goats. GH gene exon-4 region was found to be monomorphic on restriction digestion with HaeIII, which revealed only one genotype CC in both Surti and Mehsani goat breeds. The allelic frequency of C was 1.00 in both the breeds of goats with absence of D allele.

scholarly journals Polymorphism of Growth Hormone (GH) Gene in Lakor Goat from Lakor Island of Southwest Maluku Regency

2020 ◽  
Vol 44 (4) ◽  
Author(s):  
Rony Marsyal Kunda ◽  
Slamet Diah Volkandari ◽  
Maman Rumanta ◽  
Pieter Kakisina
Keyword(s):  
Growth Hormone ◽  
Polymorphic Information Content ◽  
Growth Trait ◽  
Hair Follicles ◽  
Nonrandom Mating ◽  
Economic Trait ◽  
Goat Population ◽  
Pcr Rflp ◽  
Gh Gene ◽  
Dna Fragment

Lakor goat survive in Lakor island in Southwest Maluku with high temperature and limited water. Growth trait in goat is interest to explore cause related with economic trait that encoded by growth hormone (GH) gene. The aim of this study was identify of polymorphism GH gene of Lakor goat in Lakor island. A total of 63 samples were collected from three locations (village) i.e Ketti Letpey (18), Werwawan-Yamluli (26), and Letoda (19). DNA was extracted from hair follicles. A 422 bp specific DNA fragment was successfully amplified and genotyped by PCR-RFLP method using HaeIII enzyme. Results showed that polymorphism was found with two variant of genotypes (AA and AB) and two alleles (A and B). AB genotype was dominant in all of populations (93.7%) with A and B alleles were 0.53 and 0.47, respectively. Heterozygosity observed and expected value reached 0.502 and 0.498, respectively while Polymorphic Information Content was in moderate values (0.374). All of populations were in disequilibrium genetic. It maybe caused limited buck and nonrandom mating in population that effect of low genetic variation. Inbreeding study are needed to explore it. The introgression of bucks from other families in several locations within Lakor island can be an alternative solution to increase the genetic diversity of the lakor goat population.

scholarly journals Unusual sequence element found at the end of an amplicon.

1987 ◽  
Vol 7 (7) ◽  
pp. 2636-2640 ◽  
Author(s):  
N Baran ◽  
A Lapidot ◽  
H Manor
Keyword(s):  
Cell Line ◽  
Base Pair ◽  
Specific Cell ◽  
Base Pairs ◽  
Sequence Element ◽  
Chromosomal Site ◽  
Base Pair Fragment ◽  
Amplification Process ◽  
Pair Fragment

In a polyomavirus-transformed rat cell line, designated LPT, the polyomavirus DNA is integrated into a single chromosomal site. Treatment of LPT cells with carcinogens induces amplification of the integrated virus DNA and flanking cellular sequences. We show that the amplification is arrested within a specific cell DNA segment that maps 1.3 to 1.85 kilobases beyond one virus-cell DNA junction, defined as the left junction. We also present the sequence of an 897-base-pair fragment spanning the arrest site. This fragment contains an unusual sequence element, which consists of two contiguous components, a potential cruciform with stems of 6 base pairs and a d(G-A)27 X d(T-C)27 tract, and maps 1,497 to 1,564 nucleotides beyond the left junction. The possibility that this unusual sequence plays a role in the arrest of the amplification process is discussed.

scholarly journals Investigation of Growth Hormone Releasing Hormone, Growth Hormone and Prolactin Hormone Gene Polymorphism in Anatolian Water Buffalo

2017 ◽  
Vol 17 (4) ◽  
pp. 1053-1062 ◽  
Author(s):  
Mehmet Akif Konca ◽  
Bilal Akyüz
Keyword(s):  
Growth Hormone ◽  
Water Buffalo ◽  
Pcr Amplification ◽  
Chi Square ◽  
Pcr Rflp ◽  
Gh Gene ◽  
Pcr Products ◽  
Chi Square Test ◽  
Prl Gene ◽  
Prolactin Hormone

AbstractThe purpose of this work was to identify GHRH, GH and PRL gene polymorphisms in Anatolian water buffalo by means of the PCR-RFLP method. A total of 126 buffalo were included in this study. PCR amplification gave a 451 bp band for the GHRH gene, a 221 bp band for the GH gene and a 156 bp band for the PRL gene. The PCR products were digested by HaeIII for the GHRH gene, AluI for the GH gene and RsaI for the PRL gene. The GH/AluI and PRL/RsaI polymorphisms were found to be polymorphic, while the GHRH/HaeIII polymorphism was not found in Anatolian water buffalo. The frequencies of GH-L (0.87) and PRL-A (0.55) alleles were found to be high in the examined Anatolian water buffalo. The chi-square test showed that the Anatolian water buffalo were in Hardy-Weinberg (HW) equilibrium for the GH gene while significant deviation was observed from HW equilibrium for the PRL gene. The present study is the first to examine GHRH/HaeIII, GH/AluI and PRL/RsaI polymorphisms in Anatolian water buffalo.

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