scholarly journals Investigation of Growth Hormone Releasing Hormone, Growth Hormone and Prolactin Hormone Gene Polymorphism in Anatolian Water Buffalo

2017 ◽  
Vol 17 (4) ◽  
pp. 1053-1062 ◽  
Author(s):  
Mehmet Akif Konca ◽  
Bilal Akyüz
Keyword(s):  
Growth Hormone ◽  
Water Buffalo ◽  
Pcr Amplification ◽  
Chi Square ◽  
Pcr Rflp ◽  
Gh Gene ◽  
Pcr Products ◽  
Chi Square Test ◽  
Prl Gene ◽  
Prolactin Hormone

AbstractThe purpose of this work was to identify GHRH, GH and PRL gene polymorphisms in Anatolian water buffalo by means of the PCR-RFLP method. A total of 126 buffalo were included in this study. PCR amplification gave a 451 bp band for the GHRH gene, a 221 bp band for the GH gene and a 156 bp band for the PRL gene. The PCR products were digested by HaeIII for the GHRH gene, AluI for the GH gene and RsaI for the PRL gene. The GH/AluI and PRL/RsaI polymorphisms were found to be polymorphic, while the GHRH/HaeIII polymorphism was not found in Anatolian water buffalo. The frequencies of GH-L (0.87) and PRL-A (0.55) alleles were found to be high in the examined Anatolian water buffalo. The chi-square test showed that the Anatolian water buffalo were in Hardy-Weinberg (HW) equilibrium for the GH gene while significant deviation was observed from HW equilibrium for the PRL gene. The present study is the first to examine GHRH/HaeIII, GH/AluI and PRL/RsaI polymorphisms in Anatolian water buffalo.

A minisatellite polymorphism in intron III of the barramundi (Lates calcarifer) growth hormone gene

Genome ◽  
1996 ◽  
Vol 39 (5) ◽  
pp. 934-940 ◽  
Author(s):  
David L. Yowe ◽  
Ronald J. Epping
Keyword(s):  
Growth Hormone ◽  
Variable Number Tandem Repeat ◽  
Pcr Amplification ◽  
Direct Repeat ◽  
Variable Number ◽  
Growth Hormone Gene ◽  
Lates Calcarifer ◽  
Gh Gene ◽  
Pcr Products ◽  
Heteroduplex Formation

This paper describes the detection of a polymorphism within the growth hormone (GH) gene of the fish barramundi (Lates calcarifer). PCR amplification of barramundi genomic DNA generated three different sized products: A, 409 bp; B, 478 bp; and H, 520 bp. Each barramundi isolate displayed one of four different types of profiles, which contained specific combinations of these PCR products. Sequence analysis confirmed that products A and B are different forms of the barramundi GH gene, and studies showed that product H was an artifact due to heteroduplex formation between the two smaller-sized molecules. The polymorphic nature of these PCR products was due to differences in the number of repeat monomers within the 5′ end of the barramundi decaminisatellite, an AT-rich repetitive sequence that was identified within intron III of this gene. The barramundi decaminisatellite consisted of 24 or 28 10-nucleotide imperfect direct repeat monomers in a tandem array. The monomers were grouped into one of three different families and evidence for monomer homogenization by crossover fixation was presented. The barramundi decaminisatellite differed from previously reported AT- or GC-rich minisatellites, although a similar decaminisatellite has been identified in intron III of the tilapia GH gene. Key words : mutation, PCR, somatotropin, teleost fish, variable number tandem repeat, VNTR.

scholarly journals CYP 3A5*3 Gene Polymorphism Among Sudanese Patients With Chronic Myeloid Leukemia 

2020 ◽  
Author(s):  
Elharam Ibrahim Abdallah ◽  
Nazic Ibrahim M.Alamin ◽  
Wala Eldin Osman Elradi ◽  
Salah Eldin G. Elzaki ◽  
NassrEldin Mohamed Ahmed Shrif
Keyword(s):  
Chronic Myeloid Leukemia ◽  
Myeloid Leukemia ◽  
Chronic Phase ◽  
Vital Role ◽  
Chi Square ◽  
Mutant Genotype ◽  
Therapeutic Drugs ◽  
Pcr Rflp ◽  
Chi Square Test ◽  
Healthy Control

Abstract Introduction: Interaction of environmental and genetic elements plays a vital role in the pathogenesis of CML and other types of cancer. CYP 3A5 is enzyme responsible of metabolizing of approximately 50 % of therapeutic drugs and xenobiotics. So polymorphism variation may genetically predispose individuals to CML.Objective: The purpose of this study was to determine CYP3A5*3 Polymorphism among Sudanese patients with Chronic Myeloid Leukemia (CML).Materials and Methods: This case control study was conducted among 50 patients with chronic myeloid leukemia among both genders at different ages and 42 apparently healthy control subjects. The CYP3A5*3 genotype was determined using (PCR-RFLP) method.Results: Paerson`s chi-square test was used to assess the possible link between CYP3A5*3 genotype and CML. The percentage of CYP3A5*3*3 genotype in CML patients was significantly higher than in control (OR = 11.71, 95% CI: 4.30 –31.83; p =0.000). The frequency of mutant genotype was higher among CML patients with advance phase compared with chronic phase, 100% versus 80% respectively.Conclusion: CYP3A5*3/*3 genotype associated with CML development and progression.

scholarly journals Association of growth hormone (GH) gene polymorphism with growth and carcass in Sumba Ongole (SO) cattle

2017 ◽  
Vol 42 (3) ◽  
pp. 153 ◽  
Author(s):  
P. P. Agung ◽  
S. Anwar ◽  
W. P. B. Putra ◽  
M. S. A. Zein ◽  
A. S. Wulandari ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Gene Polymorphism ◽  
Fragment Length Polymorphism ◽  
Growth Parameters ◽  
Rflp Analysis ◽  
Cattle Breeding ◽  
Pcr Rflp ◽  
Gh Gene ◽  
Polymerase Chain ◽  
Dressing Percentage

A study was conducted to identify the polymorphism in the intron 3 of the Growth Hormone (GH) gene and also to evaluate the association of the GH gene polymorphism with growth parameters and dressing percentage in the Sumba Ongole (SO) cattle. A total of 267 individual DNA samples were used in the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis. The SO cattle growth parameters data (n=44) including birth weight (BW), weaning weight at 205 days of age (WW205), yearling weight at 365 days of age (YW365) and also dressing percentage (DP) (n=122) were investigated in this study. There were three genotypes (AA, AB, and BB) of the GH gene based on the PCR-RFLP analysis with allele frequency was 0.87 and 0.13 for A allele and B allele respectively. The highest genotype frequency in the SO cattle is AA (0.76) and the lowest is BB (0.02). The Heterozygosity Observed (Ho) value in the SO cattle population is 0.23 and Polymorphism Information Content (PIC) value is 0.20. Therefore, the genetic diversity in the SO cattle based on the GH gene polymorphism is quite low. There is no association (P>0.05) in BW, WW205, YW365, and DP with genotypes of the GH gene. As the result, the GH gene in this study cannot be used as a genetic marker in the SO cattle breeding program.

Sequence analysis and PCR-RFLP of growth hormone (GH) gene in Vembur and Kilakarsal breeds of sheep

2017 ◽  
Vol 23 (2) ◽  
pp. 148
Author(s):  
M. Seevagan ◽  
V. Jeichitra ◽  
R. Rajendran ◽  
K.G. Tirumurugaan
Keyword(s):  
Growth Hormone ◽  
Sequence Analysis ◽  
Pcr Rflp ◽  
Gh Gene

scholarly journals Analysis of Prolactin and Kappa-Casein Genes Polymorphism in Four Cattle Breeds in Turkey

2014 ◽  
Vol 14 (4) ◽  
pp. 799-806
Author(s):  
Bilal Akyüz ◽  
Mehmet Ulaş Çınar
Keyword(s):  
Restriction Enzymes ◽  
Chi Square ◽  
Brown Swiss ◽  
Weinberg Equilibrium ◽  
Cattle Breeds ◽  
Genotype Frequencies ◽  
Pcr Rflp ◽  
Chi Square Test ◽  
Hardy Weinberg Equilibrium ◽  
Casein Genes

Abstract The objective of this study was to identify allele and genotype frequencies of CSN3 and PRL genes in four cattle breeds in Turkey. For this purpose, a total of 390 cattle of East Anatolian Red (EAR), Zavot, Brown Swiss (BS) and Simmental (SIM) breeds were genotyped by PCR-RFLP method. A 443 bp fragment of CSN3 and a 156 bp fragment of PRL were amplified and digested with HindIII and RsaI restriction enzymes, respectively. For CSN3 and PRL genes, two types of alleles (A and B) and three types of genotypes (AA, BB, and AB) were observed. The highest frequencies for CSN3-A and CSN3-B alleles were estimated for the EAR breed (0.743) and for the BS breed (0.556), respectively. The highest frequency for PRL-A and PRL-B alleles was estimated for the SIM breed (0.801) and for the BS breed (0.315), respectively. The Chi-square test among the investigated cattle breeds showed that only the Zavot breed was in Hardy-Weinberg equilibrium (HWE) for both loci.

scholarly journals Study on genetic variability in MHC-DRB1 second exon in Makuie sheep breed population

Genetika ◽  
2014 ◽  
Vol 46 (1) ◽  
pp. 269-275 ◽  
Author(s):  
Fereshteh Ashrafi ◽  
Ali Hashemi ◽  
Karim Mardani ◽  
Reza Darvishzadeh
Keyword(s):  
Major Histocompatibility Complex Gene ◽  
Enzymatic Digestion ◽  
Sheep Breed ◽  
Chi Square ◽  
Exon 2 ◽  
Sheep Population ◽  
Histocompatibility Complex ◽  
Pcr Products ◽  
Chi Square Test ◽  
Hardy Weinberg Equilibrium

In the present study polymorphism of the exon 2 of MHC (Major Histocompatibility Complex) gene in Makuie sheep breed was studied. Genomic DNA from blood samples of 90 sheep was extracted and a 279 bp MHC exon 2 fragment was amplified using polymerase chain reaction (PCR). PCR products were subjected to enzymatic digestion using RsaI endonuclease. Digested PCR products were electrophoresed on 2% agarose gel. The results showed the existence of 10 alleles: A, B, E, F, I, M, O, P, Q and V for the exon 2 of the MHC gene, with the frequencies of 0.4756, 0.0976, 0.0183, 0.0366, 0.0549, 0.0122, 0.1098, 0.0915, 0.0854 and 0.0183, respectively. Eighteen genotypes: AA, AB, AE, FF, AM, BO, EO, IO, OM, AP, BP, OP, PP, AQ, OQ, PQ, QQ and AV with the frequencies of 0.317, 0.1585, 0.0121, 0.0365, 0.0121, 0.0243, 0.0243, 0.1097, 0.0121, 0.0487, 0.0121, 0.0365, 0.0365, 0.0487, 0.0121, 0.0121, 0.0487 and 0.0365, respectively were identified in the population under study. Effective number of alleles and heterozygosity for the examined region were 3.7231 and 0.7314, respectively. Chi-square test showed that the examined sheep population was not in Hardy-Weinberg equilibrium in the examined region. <br><br><font color="red"><b> This article has been corrected. Link to the correction <u><a href="http://dx.doi.org/10.2298/GENRSR1602800A">10.2298/GENRSR1602800A</a><u></b></font>

scholarly journals Genetic Polymorphism of Growth Hormone Gene Exon-4 in Surti and Mehsani Goats by PCR- RFLP

2018 ◽  
Vol 14 (1) ◽  
Author(s):  
Jyotishree Bayan ◽  
Vishnu Kharadi ◽  
Umed Ramani ◽  
Mamta Janmeda ◽  
Kuldeep Tyagi ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Fragment Length Polymorphism ◽  
Allelic Frequency ◽  
Growth Hormone Gene ◽  
Chain Reaction ◽  
Pcr Rflp ◽  
Gh Gene ◽  
Polymerase Chain ◽  
Exon 4 ◽  
Gene Exon

The present investigation was planned to study growth hormone (GH) gene exon-4 polymorphism using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) in Surti and Mehsani goats. GH gene exon-4 region was found to be monomorphic on restriction digestion with HaeIII, which revealed only one genotype CC in both Surti and Mehsani goat breeds. The allelic frequency of C was 1.00 in both the breeds of goats with absence of D allele.

scholarly journals Association of MTR A2756G Gene Polymorphism with Risk of Head and Neck Cancer

2020 ◽  
Vol 1 (3) ◽  
pp. 6
Author(s):  
Muhammad Tahir ◽  
Aamer Ali Khattak ◽  
Erum Monis ◽  
Sana Gul
Keyword(s):  
Head And Neck ◽  
Age Groups ◽  
P Value ◽  
Chi Square ◽  
Normal Individuals ◽  
Increased Risk ◽  
Significant Difference ◽  
Pcr Rflp ◽  
Chi Square Test ◽  
History Of

Objective: To perform genotyping for MTR A2756G polymorphism and identification of risk factors associated with head and neck squamous cell carcinoma (HNSCC). Study Design: Cross section, comparative study. Place and Duration of Study: The study was carried out at the Department of Biochemistry of Quaid-i- Azam University, Islamabad from October 2014 to August 2015. Materials and Methods: In this study, 292 diagnosed patients HNSCC and 324 normal individuals without any history of cancer were enrolled. Blood samples of patients and controls were collected in ethylenediamine tetra acetic acid (EDTA) and DNA was extracted using conventional method. All samples were genotyped for the MTR A2756G polymorphism using PCR-RFLP. Frequency of polymorphism was compared between HNSCC patients andcontrols. MultipleLogisticRegression(MLR)andchi-squaretestwasperformedtoexaminetheassociation of MTR A2756G polymorphism with risk factor. Results: Chi-square test of independence showed statistically significant difference among the variables of age, smoking and MTR A2756G genotype (p-value<0.05). Multivariate analysis showed that smoking (adjusted OR, 3.7; 95% CI, 2.3 – 6.0), age groups 41 – 50 years (adjusted OR, 3.6; 95% CI, .9 – 6.7) and > 60 years (adjusted OR, 3.5; 95% CI, 1.7 – 7.3), MTR 2756 AG genotype (adjusted OR, 2.1; 95% CI, 1.3 – 3.5) is associated with increased risk of HNSCC. Conclusion: The results suggest that the genetic polymorphism MTR A2756G is associated with the occurrence of HNSCC in the Pakistani population while the individuals between 40 to 50 years of age and those who are smokers are at a greater risk of developing HNSCC.

scholarly journals The Association Between TP53 rs1625895 Polymorphism and the Risk of Sarcopenic Obesity in Iranian Older Adults: A Case-Control Study

2020 ◽  
Author(s):  
Nima Montazeri-Najababady ◽  
Mohammad Hossein Dabbaghmanesh ◽  
Nasrin Nasimi ◽  
Zahra Sohrabi ◽  
Nazanin Chatrabnous
Keyword(s):  
Case Control Study ◽  
Handgrip Strength ◽  
Sarcopenic Obesity ◽  
P Value ◽  
Anthropometric Parameters ◽  
Chi Square ◽  
Pcr Rflp ◽  
Chi Square Test ◽  
Adjusted Model ◽  
Control Study

Abstract Background: Aging and obesity are the two major global health concerns. Sarcopenia, an age-linked disease, wherein a progressive loss of muscle volume, muscle strength, and physical activity occurs. In this study we evaluated the association of TP53 rs1625895 polymorphism with the susceptibility to sarcopenic obesity in Iranian old-age subjects. Total of 206 old individuals (45 sarcopenic and 161 non-sarcopenic) were recruited in this research and genotyped by PCR–RFLP. BMI, Skeletal Muscle Mass Index (SMI), body composition, Handgrip Strength (HGS), Gait Speed (GS), and biochemical parameters were measured. Chi-square test was done for genotypes and alleles frequency. Linear regression was applied to find the correlation between TP53 rs1625895 polymorphism, and biochemical and anthropometric parameters. The correlation between TP53 rs1625895 and the risk of sarcopenia and sarcopenic obesity was investigated by logistic regression.Results: G allele was significantly higher in sarcopenic obesity group [P =0.037, OR (CI 95%)=1.9 (1.03-3.5)] compared to A allele. BMI (P= 0.049) and LDL (P=0.04) were significantly differed between genotypes when GG was compared to AA/AG genotype. The results revealed when GG genotype compared to AA/AG genotype in adjusted model for age, the risk of sarcopenic obesity [P value= 0.011, OR (CI 95%); 2.72 (1.25-5.91)] increased. Similarly, GG/AG genotype increased the risk of sarcopenic obesity [P value= 0.028, OR (CI 95%); 2.43 (1.10-5.36)] in adjusted model for age compared to AA genotype.Conclusion: We concluded that TP53 rs1625895 polymorphism may increase the risk of sarcopenic obesity in Iranian population.

scholarly journals Polymorphism of Growth Hormone (GH) Gene in Lakor Goat from Lakor Island of Southwest Maluku Regency

2020 ◽  
Vol 44 (4) ◽  
Author(s):  
Rony Marsyal Kunda ◽  
Slamet Diah Volkandari ◽  
Maman Rumanta ◽  
Pieter Kakisina
Keyword(s):  
Growth Hormone ◽  
Polymorphic Information Content ◽  
Growth Trait ◽  
Hair Follicles ◽  
Nonrandom Mating ◽  
Economic Trait ◽  
Goat Population ◽  
Pcr Rflp ◽  
Gh Gene ◽  
Dna Fragment

Lakor goat survive in Lakor island in Southwest Maluku with high temperature and limited water. Growth trait in goat is interest to explore cause related with economic trait that encoded by growth hormone (GH) gene. The aim of this study was identify of polymorphism GH gene of Lakor goat in Lakor island. A total of 63 samples were collected from three locations (village) i.e Ketti Letpey (18), Werwawan-Yamluli (26), and Letoda (19). DNA was extracted from hair follicles. A 422 bp specific DNA fragment was successfully amplified and genotyped by PCR-RFLP method using HaeIII enzyme. Results showed that polymorphism was found with two variant of genotypes (AA and AB) and two alleles (A and B). AB genotype was dominant in all of populations (93.7%) with A and B alleles were 0.53 and 0.47, respectively. Heterozygosity observed and expected value reached 0.502 and 0.498, respectively while Polymorphic Information Content was in moderate values (0.374). All of populations were in disequilibrium genetic. It maybe caused limited buck and nonrandom mating in population that effect of low genetic variation. Inbreeding study are needed to explore it. The introgression of bucks from other families in several locations within Lakor island can be an alternative solution to increase the genetic diversity of the lakor goat population.

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